##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077461_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 252612 Sequences flagged as poor quality 0 Sequence length 50 %GC 30 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.129332731620035 31.0 30.0 33.0 23.0 34.0 2 28.87899228856903 31.0 28.0 34.0 20.0 34.0 3 29.146002565198803 31.0 28.0 34.0 23.0 34.0 4 32.44970943581461 35.0 32.0 37.0 23.0 37.0 5 32.48794594081041 35.0 32.0 37.0 25.0 37.0 6 32.01717258087502 36.0 32.0 37.0 17.0 37.0 7 31.824588697290707 36.0 32.0 37.0 16.0 37.0 8 31.6318781372223 37.0 32.0 37.0 12.0 37.0 9 32.68415593875192 38.0 32.0 39.0 10.0 39.0 10 32.979783224866594 38.0 32.0 39.0 15.0 39.0 11 32.74376118315836 38.0 32.0 39.0 12.0 39.0 12 32.94156651307143 38.0 32.0 39.0 15.0 39.0 13 32.618106028217184 37.0 32.0 39.0 11.0 39.0 14 33.431745126914 38.0 32.0 41.0 12.0 41.0 15 33.42830902728295 38.0 32.0 40.0 12.0 41.0 16 33.459241841242694 38.0 32.0 40.0 13.0 41.0 17 33.74262901208177 38.0 32.0 40.0 16.0 41.0 18 33.829343024084366 38.0 32.0 41.0 17.0 41.0 19 33.90683340458886 39.0 32.0 41.0 18.0 41.0 20 34.04455449464 39.0 33.0 41.0 18.0 41.0 21 33.92856238025114 39.0 33.0 41.0 18.0 41.0 22 33.92279860022485 39.0 33.0 41.0 18.0 41.0 23 33.67815068167783 38.0 32.0 41.0 18.0 41.0 24 33.61515684132187 38.0 32.0 41.0 17.0 41.0 25 33.48007220559593 38.0 32.0 41.0 16.0 41.0 26 33.15066188462939 37.0 32.0 41.0 12.0 41.0 27 32.8120081389641 37.0 32.0 40.0 9.0 41.0 28 32.69964213893243 37.0 32.0 40.0 8.0 41.0 29 32.37304641109686 36.0 31.0 40.0 2.0 41.0 30 31.897538517568446 36.0 31.0 39.0 2.0 41.0 31 31.349627096099947 35.0 30.0 39.0 2.0 41.0 32 30.759370101182842 35.0 30.0 38.0 2.0 41.0 33 30.451391857868984 35.0 30.0 38.0 2.0 41.0 34 30.184773486611878 35.0 30.0 38.0 2.0 41.0 35 29.962028723892768 35.0 29.0 38.0 2.0 41.0 36 29.60949598593891 35.0 27.0 38.0 2.0 41.0 37 29.42418412426963 35.0 26.0 38.0 2.0 40.0 38 29.275802416353933 35.0 25.0 38.0 2.0 40.0 39 28.99239941095435 35.0 25.0 37.0 2.0 40.0 40 28.859543489620446 35.0 25.0 37.0 2.0 40.0 41 28.704618149573257 35.0 25.0 37.0 2.0 40.0 42 28.4579394486406 35.0 25.0 37.0 2.0 40.0 43 28.30406710686745 35.0 24.0 38.0 2.0 40.0 44 27.98778363656517 35.0 22.0 38.0 2.0 40.0 45 27.658088293509415 35.0 22.0 38.0 2.0 41.0 46 27.03749228065175 35.0 20.0 38.0 2.0 40.0 47 26.59895016863807 34.0 19.0 38.0 2.0 40.0 48 26.142879198137855 33.0 13.0 37.0 2.0 40.0 49 25.939143825313128 33.0 2.0 37.0 2.0 40.0 50 25.853360093740598 33.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9085247950281072 1101 2 1.0177020011321964 1101 3 0.9531315617114622 1101 4 0.9262378598164176 1101 5 0.521160734165143 1101 6 0.34951378078416795 1101 7 0.7612720006073879 1101 8 0.4286150956626784 1101 9 0.859900469819145 1101 10 0.879274127766557 1101 11 0.8627723956079265 1101 12 0.5570085972599799 1101 13 1.0418660508424225 1101 14 0.9140129828425856 1101 15 1.1498690005945065 1101 16 1.2286900181463594 1101 17 0.7865489122627665 1101 18 1.38165535489874 1101 19 1.1810034878028048 1101 20 1.4166191507815995 1101 21 1.0879777604103609 1101 22 1.1817907227135294 1101 23 1.3400059933880613 1101 24 1.2812945350992706 1101 25 1.435200095614455 1101 26 1.56983064229442 1101 27 1.541771141890429 1101 28 1.4312436506333484 1101 29 1.7410122528297123 1101 30 1.8052517074754277 1101 31 1.6561587475942403 1101 32 2.019755565078306 1101 33 2.085382987631945 1101 34 2.053094183238997 1101 35 1.8581239821008815 1101 36 1.4160407391161982 1101 37 1.5060912327156437 1101 38 1.1690045204992785 1101 39 1.2608757751268698 1101 40 1.308343506694282 1101 41 1.1657233100097955 1101 42 1.4862130849131496 1101 43 1.326021365647879 1101 44 1.6560990007504692 1101 45 1.9433450157080685 1101 46 2.2063263361941914 1101 47 2.0073929778019846 1101 48 2.1062272493316527 1101 49 2.181930952214369 1101 50 2.0644781520726134 1102 1 0.7820066093617335 1102 2 0.7227669615698247 1102 3 0.31901023710464216 1102 4 0.7932317707929428 1102 5 0.6897241153640579 1102 6 0.49442928337281344 1102 7 0.6426093064587057 1102 8 0.2129350461690116 1102 9 0.49633366964982173 1102 10 0.9480198540535554 1102 11 0.6123735157277537 1102 12 0.8251372807935695 1102 13 1.0966754025405123 1102 14 0.5503077957760567 1102 15 0.9335865610777176 1102 16 0.6919116651132455 1102 17 0.8914950227769154 1102 18 0.7603307480803352 1102 19 1.094959399365436 1102 20 0.9566373852904064 1102 21 0.85387597277726 1102 22 0.7113810974978065 1102 23 0.9194889148168599 1102 24 1.0744631066208754 1102 25 0.8784858889783962 1102 26 1.1682318666114355 1102 27 1.0191652352120428 1102 28 1.3053766974243999 1102 29 1.1758157434356775 1102 30 1.3660930998104206 1102 31 1.023120096947892 1102 32 0.982057346199074 1102 33 1.2273598037564604 1102 34 1.2338193305803387 1102 35 1.3579041911465026 1102 36 1.1570130291751362 1102 37 1.0446523346009613 1102 38 1.266778933576024 1102 39 1.1085932053635936 1102 40 1.051025281953958 1102 41 1.0368444066852511 1102 42 1.0454589577274511 1102 43 0.9237713575074729 1102 44 1.30265886529412 1102 45 1.1641698016153974 1102 46 1.2301044612959444 1102 47 1.5883900146943013 1102 48 1.5380106494163144 1102 49 1.6381518502639274 1102 50 1.5890784581518709 1103 1 1.0480936513108432 1103 2 1.16227477176729 1103 3 1.0247278910981592 1103 4 0.7885898609786182 1103 5 1.0649328785038534 1103 6 1.2314859687053605 1103 7 1.0464063395660865 1103 8 1.1408012412765842 1103 9 1.418280815734903 1103 10 1.2370217206416285 1103 11 1.0654632101967607 1103 12 1.1522322803023641 1103 13 1.4470781925984753 1103 14 1.5965549713335818 1103 15 1.4834686713046565 1103 16 1.5998594006471833 1103 17 1.6726130107914514 1103 18 1.6019163655280195 1103 19 1.44317631654787 1103 20 1.5498685474870868 1103 21 1.7455647437763773 1103 22 1.6892522046792564 1103 23 1.9436071975558349 1103 24 1.8043972848309124 1103 25 1.713563008538273 1103 26 1.764410273134672 1103 27 1.9732619048259536 1103 28 2.0781932805858077 1103 29 2.12028474824951 1103 30 2.0937677240367663 1103 31 1.9521160690473138 1103 32 1.867483223004264 1103 33 2.056788038357112 1103 34 1.9999800536376142 1103 35 2.0164413755457566 1103 36 1.817588724233591 1103 37 1.5510134702724407 1103 38 1.2425378489901107 1103 39 1.373132944041263 1103 40 1.3971791281078048 1103 41 1.3698830155859305 1103 42 1.3034410777785368 1103 43 1.9562401658383521 1103 44 1.6452760182561015 1103 45 1.7414612840792927 1103 46 1.88299276073597 1103 47 1.8429336830310739 1103 48 1.4381481874848845 1103 49 1.4563068753155015 1103 50 1.941470638130255 1104 1 0.35598945920665415 1104 2 0.3171384266309438 1104 3 0.4594043257745959 1104 4 0.6681177405064957 1104 5 0.7902076037785832 1104 6 0.753870991090384 1104 7 0.8286611218208684 1104 8 0.7468248473001893 1104 9 0.3773772748313604 1104 10 0.22420120782111042 1104 11 0.78259792733148 1104 12 0.9365220645921468 1104 13 1.0647013102215936 1104 14 0.8368887116673207 1104 15 0.6163543041902884 1104 16 1.1916379924257754 1104 17 1.0574318956103355 1104 18 1.1808744444861006 1104 19 1.1564445298160848 1104 20 0.7509267485452895 1104 21 0.8401923384039733 1104 22 0.6566921721192216 1104 23 0.47677773072636853 1104 24 0.8947513751850025 1104 25 0.5983818933571641 1104 26 0.8523223610467596 1104 27 0.9527083842724338 1104 28 0.9206841230766543 1104 29 0.9776311055958686 1104 30 1.0066696369386818 1104 31 0.7817863987176423 1104 32 0.5954034509244899 1104 33 0.8207278022968758 1104 34 0.6233011769587371 1104 35 0.8644267235311034 1104 36 1.0475439541888214 1104 37 0.8100692293281995 1104 38 1.0575561640084281 1104 39 1.0094780803863976 1104 40 1.0536707845994613 1104 41 0.8833140290169439 1104 42 0.9092611835986446 1104 43 0.9845266941248809 1104 44 0.8941567671368489 1104 45 0.4744690170870278 1104 46 0.5413159190591266 1104 47 0.29327963337702556 1104 48 0.12658937592607344 1104 49 0.8940358130444395 1104 50 0.9671116637712807 1105 1 0.18140814826170626 1105 2 0.012949349714595826 1105 3 -0.18089476906995472 1105 4 -0.11626286205591896 1105 5 0.39906675809228176 1105 6 0.09369783091722539 1105 7 0.3441624555040228 1105 8 0.12038731177174711 1105 9 0.1713122960391118 1105 10 0.48024650932096336 1105 11 0.3153278236977357 1105 12 -0.03973400257301307 1105 13 0.07727291370228784 1105 14 0.2587479971629705 1105 15 0.4268182510178704 1105 16 0.29645236996742597 1105 17 0.3649442485772383 1105 18 0.1487130032337518 1105 19 -0.09203594593711983 1105 20 0.22418749453330378 1105 21 0.34950625680880165 1105 22 0.48593700136405715 1105 23 0.8349919071223653 1105 24 0.7522440508594954 1105 25 0.39793587472932046 1105 26 0.4582955124744572 1105 27 0.6787786103426612 1105 28 0.8378645857116567 1105 29 0.1332347611995246 1105 30 0.26159989186893995 1105 31 0.44016673891616875 1105 32 0.7559482932875135 1105 33 0.8489756487265403 1105 34 0.86084982359829 1105 35 0.8144682644817358 1105 36 0.912688910242867 1105 37 0.7021283486600467 1105 38 0.5490293372997819 1105 39 0.5748941458024639 1105 40 0.9752677061963837 1105 41 0.8232982871830217 1105 42 1.0530584637595588 1105 43 0.7226219322201182 1105 44 1.02798421914612 1105 45 0.9469426713788636 1105 46 1.0340134572112127 1105 47 0.9374923230442604 1105 48 1.038675606194122 1105 49 1.089562508571138 1105 50 0.9401756368352565 1106 1 1.0205734961311137 1106 2 1.1877339461626342 1106 3 1.1991459671119813 1106 4 1.2058020813823305 1106 5 1.075851530379957 1106 6 1.560735226146111 1106 7 1.0978550878233442 1106 8 0.9319678170176289 1106 9 1.1194689105400215 1106 10 1.3365928547872272 1106 11 1.0245769884594722 1106 12 1.4808905211946453 1106 13 0.9271351726554542 1106 14 1.1092769813960146 1106 15 1.1669565781968174 1106 16 1.1469740275490494 1106 17 1.14400174813764 1106 18 1.0210607554170892 1106 19 1.2893528941074237 1106 20 1.3930521566281442 1106 21 1.3091217541418985 1106 22 1.5637899994298152 1106 23 1.309590406624153 1106 24 1.3384640848551612 1106 25 1.3218705796118044 1106 26 1.3625947915319543 1106 27 1.1475890552169332 1106 28 1.2373282748271848 1106 29 0.8196467534625853 1106 30 1.1866482477683604 1106 31 1.2156488325800794 1106 32 1.0520622373705102 1106 33 1.3165625428124663 1106 34 1.1060069070048897 1106 35 1.2409746240151733 1106 36 1.4683055212269842 1106 37 1.2818130095826206 1106 38 1.0218840937618907 1106 39 1.0544234817147782 1106 40 1.3320536507482856 1106 41 1.2479290239510874 1106 42 0.9004662944846054 1106 43 1.0391112939222467 1106 44 1.2656388114912325 1106 45 0.8978760867706939 1106 46 0.821049680175868 1106 47 1.096063039883834 1106 48 1.042932390673343 1106 49 1.0236372990289055 1106 50 1.3703819553394467 1107 1 0.05521615843335326 1107 2 -0.04969104019852111 1107 3 -0.24279347642320914 1107 4 0.12705139944015542 1107 5 -0.19290187933090408 1107 6 -0.34173340451400946 1107 7 -0.21057371741396835 1107 8 -0.4933867929114477 1107 9 -0.3255938281397448 1107 10 -0.11312072950082097 1107 11 0.059765204498752666 1107 12 -0.1380811100110293 1107 13 -0.47542892990864516 1107 14 -0.25415727937866706 1107 15 -0.2353347474987615 1107 16 -0.33204943126164466 1107 17 -0.656161917983475 1107 18 -0.5844084207967626 1107 19 -0.45084077746922446 1107 20 -0.1404448428263052 1107 21 -0.5339224357107994 1107 22 -0.49051987509282213 1107 23 -0.4663643124156778 1107 24 -0.36682778639416114 1107 25 -0.1354398404645707 1107 26 0.015977224701622106 1107 27 -0.14639621483216558 1107 28 -0.33405743166490254 1107 29 -0.10079787283310537 1107 30 0.21428054454958811 1107 31 0.13359755052879763 1107 32 -0.24362788810684677 1107 33 -0.1936393120702391 1107 34 -0.32281196915441157 1107 35 -0.0881170290126505 1107 36 -0.04793834129347374 1107 37 -0.5239153046563345 1107 38 -0.548915142462878 1107 39 -0.42487275396443636 1107 40 -0.45410292317424705 1107 41 -0.546356300653386 1107 42 -0.4372996629622037 1107 43 -0.6978908882927009 1107 44 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9705.0 3 1346.0 4 2510.0 5 1273.0 6 1014.0 7 834.0 8 779.0 9 788.0 10 975.0 11 1175.0 12 1362.0 13 1591.0 14 1662.0 15 1585.0 16 1516.0 17 1480.0 18 1481.0 19 1378.0 20 1744.0 21 2620.0 22 4422.0 23 5329.0 24 4492.0 25 3964.0 26 3879.0 27 3997.0 28 4576.0 29 5212.0 30 6017.0 31 6998.0 32 8700.0 33 11123.0 34 15153.0 35 26325.0 36 32568.0 37 23579.0 38 19294.0 39 30166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.939224047480465 18.44619447512937 17.810323597302894 13.804257880087265 2 10.594154648847438 29.426272015074563 37.518952064636935 22.46062127144107 3 20.715249929733858 37.69115359188634 30.28332099552276 11.31027548285704 4 8.029436914111985 21.85678996706359 33.9090606789967 36.204712439827716 5 7.081103986883325 45.31154085297997 30.27092747412902 17.336427686007692 6 20.527359913835774 42.10920207015891 17.342530519796785 20.020907496208537 7 14.8190972483458 43.55327295982006 21.82474786072646 19.80288193110767 8 24.824292915094375 29.071355884554013 21.491500726585915 24.612850473765697 9 24.269518767398303 21.135745370022054 22.72480686151446 31.869929001065184 10 17.989189617280772 33.18629100916704 24.749044686875088 24.075474686677094 11 20.648429022881537 31.06273612178142 24.93683618593526 23.351998669401787 12 29.305749711900585 35.795213787586576 25.803015242538123 9.09602125797471 13 30.97487763539307 26.15276171769812 27.660737197256495 15.21162344965231 14 23.584412498514908 26.932794740802347 38.246802106847255 11.235990653835492 15 24.34728200822902 27.238922703458325 35.78157856178298 12.632216726529673 16 9.514587714399998 30.32668401272044 41.57112476089772 18.587603511981847 17 14.746476251579201 28.167809236399066 37.245793085967975 19.83992142605376 18 12.758921145391897 22.39692661095489 50.05940829339776 14.784743950255455 19 12.461136920816353 26.802355746190926 51.7784933205011 8.958014012491633 20 12.042867666278545 22.052451900609114 50.3073291669637 15.597351266148642 21 12.811982669169659 22.579584788790406 52.70695213427433 11.901480407765606 22 10.326295737442624 22.23119908436006 47.76852368901509 19.67398148918222 23 10.174451991049683 24.59357240450684 52.80964733371616 12.422328270727313 24 15.086015714104676 23.415520846534026 49.86020466354074 11.638258775820555 25 10.91213660862437 24.29103783111632 50.770443178083056 14.02638238217625 26 19.295626277187257 19.674624170323316 51.752023698259066 9.277725854230361 27 10.154133730416461 21.138736198457078 54.36461419044149 14.342515880684969 28 11.935859457918164 23.906569296814357 54.56104361049947 9.596527634768007 29 12.070522263935752 19.622122954585056 54.56842338703291 13.738931394446293 30 9.700068112337837 27.02673805262074 55.49294324499849 7.780250590042927 31 16.395409617943358 21.10617436482291 49.62301210162833 12.8754039156054 32 9.062938059825608 25.128101562561874 52.953265698876194 12.855694678736329 33 12.449118583398008 22.49592156738521 51.97586201434975 13.079097834867035 34 10.646874121471532 22.430935288830465 52.371581410634604 14.550609179063404 35 11.350929709525168 22.480914821502108 53.83326998004372 12.334885488929013 36 13.345238708042464 21.0562786332841 53.263352960043086 12.335129698630354 37 14.274797372474332 22.26863638703342 53.46080290785843 9.995763332633821 38 15.980344797548247 20.317635040427 52.795441766909256 10.9065783951155 39 14.275024053023555 19.05157839306006 53.608349467666 13.06504808625038 40 12.180164772299884 22.134201139391347 54.500754183640744 11.18487990466802 41 10.500290981935366 21.719920979599593 53.78098363770968 13.998804400755366 42 12.882230914187081 24.364598014220338 51.72568053334178 11.027490538250802 43 11.801824983670157 21.589042180479403 51.85724748124543 14.751885354605015 44 12.220902612826604 25.313935075217735 49.99524940617577 12.46991290577989 45 13.909232705995993 19.980047663913982 49.35075731783596 16.75996231225406 46 11.117972835488839 23.769145200685646 51.56744217347759 13.54543979034793 47 13.915126083686316 21.386326748743123 47.0927516725387 17.605795495031867 48 12.818969953683542 22.737421321404536 48.67503265903962 15.768576065872294 49 15.093503079822018 21.23177046221082 48.906227732649285 14.76849872531788 50 11.336753598403876 23.409022532579606 51.25211787246845 14.002105996548067 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12785.0 1 8247.0 2 3709.0 3 6182.0 4 8655.0 5 8039.5 6 7424.0 7 5132.5 8 2841.0 9 3358.0 10 3875.0 11 5791.0 12 7707.0 13 13228.5 14 18750.0 15 20530.5 16 22311.0 17 19294.5 18 16278.0 19 12091.0 20 7904.0 21 6521.5 22 5139.0 23 4487.5 24 3836.0 25 3537.0 26 3238.0 27 3021.5 28 2805.0 29 2661.5 30 2518.0 31 2442.5 32 2367.0 33 2600.0 34 2833.0 35 2789.5 36 2746.0 37 2548.0 38 2350.0 39 2340.0 40 2330.0 41 2339.0 42 2348.0 43 3228.0 44 4108.0 45 7449.0 46 10790.0 47 26185.5 48 41581.0 49 32886.0 50 24191.0 51 18856.5 52 13522.0 53 8405.5 54 3289.0 55 2810.0 56 2331.0 57 2026.5 58 1722.0 59 1478.0 60 1234.0 61 1047.0 62 860.0 63 841.5 64 823.0 65 688.0 66 553.0 67 493.0 68 433.0 69 441.5 70 450.0 71 447.0 72 444.0 73 416.0 74 388.0 75 358.0 76 328.0 77 304.0 78 280.0 79 249.5 80 219.0 81 180.5 82 142.0 83 118.5 84 95.0 85 67.0 86 39.0 87 29.5 88 20.0 89 15.5 90 11.0 91 7.5 92 4.0 93 4.0 94 4.0 95 2.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01781388057574462 2 3.958640127943249E-4 3 3.958640127943249E-4 4 0.0015834560511772996 5 0.04314917739458141 6 0.028106344908397064 7 0.028898072933985715 8 0.02414770478045382 9 0.028898072933985715 10 0.030481528985163018 11 0.03721121720266654 12 0.037607081215460865 13 0.03483603312590059 14 0.04037812930502114 15 0.03602362516428356 16 0.04156572134340412 17 0.04394090542017006 18 0.04829540956090764 19 0.04869127357370196 20 0.04512849745855304 21 0.04512849745855304 22 0.04394090542017006 23 0.04235744936899276 24 0.03958640127943249 25 0.037607081215460865 26 0.041169857330609794 27 0.041169857330609794 28 0.041169857330609794 29 0.03800294522825519 30 0.03483603312590059 31 0.033252577074723295 32 0.030877392997957342 33 0.025335296818836794 34 0.023355976754865167 35 0.023751840767659495 36 0.02573116083163112 37 0.02177252070368787 38 0.022960112742070844 39 0.01781388057574462 40 0.008313144268680824 41 0.007521416243092173 42 0.006333824204709198 43 0.002771048089560274 44 0.004750368153531899 45 0.003958640127943249 46 0.0011875920383829747 47 7.917280255886498E-4 48 7.917280255886498E-4 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 252612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.229854480388898 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7006955351133 24.47548018304752 2 6.322919003814224 3.569901667379222 3 2.3109715054969713 1.9571516792551424 4 1.1148193852367063 1.258847560685953 5 0.6674893426071349 0.9421563504504932 6 0.4837895445366839 0.8194385064842525 7 0.3014920350011218 0.595775339255459 8 0.23838905093111956 0.5383750574002819 9 0.2019295490240072 0.5130397605814451 >10 1.2662665470047119 6.8737827181606574 >50 0.1696769127215616 3.271420201732301 >100 0.15004487323311644 9.540718572356024 >500 0.03225263630244559 6.1612274951308725 >1k 0.030850347767556654 18.15432362674774 >5k 0.005609154139555755 10.357386030750716 >10k+ 0.0028045770697778774 10.97097525058192 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15939 6.309676499928744 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11775 4.661298750653176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8883 3.516460025651988 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6436 2.547780786344275 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5774 2.2857188098744317 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5071 2.0074264088800216 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4678 1.8518518518518516 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4088 1.6182920843032003 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3563 1.4104634775861795 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3538 1.4005668772663216 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3502 1.3863157728057258 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2929 1.1594856934745776 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2246 0.8891105727360538 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.7905404335502668 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1996 0.7901445695374725 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1917 0.7588713125267208 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1549 0.6131933558184093 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1505 0.595775339255459 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1434 0.5676689943470619 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 1432 0.5668772663214732 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1424 0.5637103542191186 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1276 0.5051224803255585 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1199 0.4746409513403956 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1198 0.4742450873276012 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1137 0.45009738254714743 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1115 0.4413883742656723 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1112 0.44020078222728926 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1025 0.405760613114183 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 948 0.37527908412902 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 904 0.3578610675660697 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 869 0.3440058271182683 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 849 0.3360885468623818 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 829 0.3281712666064953 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 790 0.31273257010751665 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 759 0.3004607857108926 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 691 0.2735420328408785 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 672 0.26602061659778636 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 645 0.25533228825233956 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 644 0.2549364242395452 RNA PCR Primer, Index 33 (95% over 22bp) ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 640 0.25335296818836794 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 624 0.24701914398365873 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 622 0.24622741595807007 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.24543568793248144 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 591 0.233955631561446 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 588 0.23276803952306302 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 585 0.23158044748468007 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 0.21851693506246733 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 545 0.21574588697290706 No Hit ACACAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 538 0.21297483888334678 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 532 0.21059965480658085 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 527 0.20862033474260921 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 481 0.19041059015407027 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 479 0.1896188621284816 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 471 0.18645195002612702 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 468 0.18526435798774404 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 418 0.1654711573480278 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 397 0.157158013079347 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 396 0.15676214906655267 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 391 0.15478282900258103 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 385 0.1524076449258151 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 369 0.1460738207211059 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 360 0.14251104460595695 RNA PCR Primer, Index 33 (95% over 24bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 360 0.14251104460595695 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 359 0.14211518059316264 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 352 0.13934413250360236 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 350 0.1385524044780137 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 350 0.1385524044780137 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 347 0.13736481243963072 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 346 0.13696894842683643 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 344 0.13617722040124777 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 343 0.13578135638845343 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 333 0.13182271626051018 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 332 0.13142685224771586 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 329 0.13023926020933288 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 321 0.1270723481069783 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 314 0.12430130001741801 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 311 0.12311370797903504 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 301 0.1191550678510918 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 286 0.11321710765917692 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 280 0.11084192358241096 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 277 0.109654331544028 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 276 0.10925846753123367 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 273 0.1080708754928507 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 269 0.10648741944167339 No Hit ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT 264 0.10450809937770178 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 3.958640127943249E-4 0.0 14 0.0 0.0 0.0 7.917280255886498E-4 0.0 15 0.0 0.0 0.0 7.917280255886498E-4 0.0 16 0.0 0.0 0.0 7.917280255886498E-4 0.0 17 0.0 0.0 0.0 0.0019793200639716246 0.0 18 0.0 0.0 0.0 0.002771048089560274 0.0 19 0.0 0.0 0.0 0.003562776115148924 0.0 20 0.0 0.0 0.0 0.003562776115148924 0.0 21 0.0 0.0 0.0 0.007125552230297848 0.0 22 0.0 0.0 0.0 0.011084192358241096 0.0 23 0.0 0.0 0.0 0.020584928665304893 0.0 24 0.0 0.0 0.0 0.032856713061928965 0.0 25 0.0 0.0 0.0 0.03602362516428356 0.0 26 0.0 0.0 0.0 0.047107817522524666 0.0 27 0.0 0.0 0.0 0.05225404968885088 0.0 28 0.0 0.0 0.0 0.07046379427738983 0.0 29 0.0 0.0 0.0 0.09342390701946067 0.0 30 0.0 0.0 0.0 0.1270723481069783 0.0 31 0.0 0.0 0.0 0.16151251722008456 0.0 32 0.0 0.0 0.0 0.2082244707298149 0.0 33 0.0 0.0 0.0 0.26166611245704874 0.0 34 0.0 0.0 0.0 0.32025398635060887 0.0 35 0.0 0.0 0.0 0.3962598768071192 0.0 36 0.0 0.0 0.0 0.4667236710845091 0.0 37 0.0 0.0 0.0 0.5419378335154308 0.0 38 0.0 0.0 0.0 0.6341741484965084 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACAAC 50 1.3296813E-9 39.62996 1 TACAAGG 35 7.239867E-6 37.73534 2 GAACAAA 285 0.0 37.08066 1 TACATAG 30 1.2939634E-4 36.687134 2 AGCGTAC 30 1.2984966E-4 36.665348 11 GTACAAA 25 0.0023405787 35.226627 1 CGTACTT 35 3.2113912E-4 31.427443 13 GTTATCA 55 4.9116825E-6 28.021183 1 TTATCAA 55 4.919262E-6 28.01563 2 TCAAGCA 40 6.9937704E-4 27.515354 2 GCAGCGT 40 7.0181105E-4 27.49901 9 GCGTACT 40 7.0181105E-4 27.49901 12 CAGCGTA 40 7.0181105E-4 27.49901 10 GTACATA 65 5.8611295E-7 27.097408 1 ATCGTAT 50 8.329057E-5 26.393826 39 ATATCGT 50 8.329057E-5 26.393826 37 GCGGTAT 45 0.0013890209 24.462938 1 ACGCAGC 45 0.0013954495 24.443565 7 TATCGTA 45 0.0013970605 24.438726 38 TATACAC 400 0.0 24.213509 3 >>END_MODULE