FastQCFastQC Report
Mon 6 Feb 2017
SRR2077460_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077460_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560752
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.024.7022251
GTATCAA36400.023.8740841
ATCAACG46850.018.2660873
TCAACGC47050.018.2351994
TATCAAC47300.018.1856842
CAACGCA48550.017.5355245
AACGCAG49800.016.9631846
GTACATG63300.016.3352241
TACATGG63050.015.7015112
TATACCG850.002982330715.5284955
ACGCAGA55850.014.8108057
CGCACGA1809.057476E-814.6675235
ACATGGG67650.014.6660873
GCACCGT3600.014.6660866
CGCAGAG58600.014.0406758
AGAGTAC55150.013.7624611
GTATAAT5500.013.600261
TAGGCCG1952.40796E-713.53898523
CCCTATA2309.16225E-913.3910362
GTCTAGA4450.013.348571
Produced by FastQC (version 0.11.5)