FastQCFastQC Report
Mon 6 Feb 2017
SRR2077460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2560752
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.10973339081644766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38800.022.9670981
GGTATCA18400.022.8401181
CGTGCCG2900.018.96740310
TGCGATA2800.017.28810913
GCGATAC3000.016.86900314
GCACCGT4050.016.8401956
AATACCG800.001987068216.502615
TGTCGCG800.001987327616.50228518
GTGCCGA3500.016.34448211
GGTGCGA3250.016.24776811
TATCAAC55600.015.7488442
TCAACGC56900.015.6603024
ATCAACG57350.015.5757873
CAACGCA56950.015.5318655
GTCGACG850.002979182215.53095611
CGATACT3200.015.12709515
AACGCAG59400.014.9265376
AGCACCG5500.014.802345
CCGATCG900.00435676414.66869814
GCACGGA4050.014.66812522
Produced by FastQC (version 0.11.5)