FastQCFastQC Report
Mon 6 Feb 2017
SRR2077459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544004
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.025.1415981
GTATCAA36350.023.6026691
GTACCGA2750.020.0005726
TACCGAT2850.019.2995577
CGAATCG2100.018.85731137
TATCAAC48250.017.599122
TCAACGC48550.017.4917474
CAACGCA49150.017.2782155
ATCAACG49500.017.1543583
AACGCAG51350.016.4951176
GTACTAA3250.016.245341
ATACCGA953.3474012E-416.2109896
CGACTCA2500.015.84076521
GTACATG69050.015.1013431
ACCGTAT1951.5163096E-814.6679538
GTTCGCG900.00436107614.66650916
GAACGCG900.004361646214.6662217
ACCGATC3950.014.4822838
TATACTG5700.014.2811115
TACATGG70500.014.2290752
Produced by FastQC (version 0.11.5)