FastQCFastQC Report
Mon 6 Feb 2017
SRR2077459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544004
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27510.10813662242669429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.027.45561
GTATCAA41650.024.8821091
CGCTACG5000.018.92143828
CGCTATC4900.018.85412633
CGCACGA1404.096364E-918.85412635
TACGCTA4850.018.59825331
CTCGTAT4950.017.77318639
ACCGTCG902.2152963E-417.112418
TATCAAC60700.016.9633962
GACGCTA5450.016.95608326
TCTCGTA5450.016.95008538
ATCTCCG6250.016.89794710
CGAGACG5650.016.74529523
ATCAACG61800.016.5902583
ACGCTAT5450.016.54976532
TCAACGC61750.016.5327554
ACGCTAC5600.016.50190427
CAACGCA63200.016.1885745
TATGCCG5400.015.88291443
TATCTCG5700.015.82171836
Produced by FastQC (version 0.11.5)