##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2544004
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.632349634670387	31.0	31.0	34.0	27.0	34.0
2	30.58850968787785	31.0	31.0	34.0	27.0	34.0
3	30.769743679648304	31.0	31.0	34.0	27.0	34.0
4	34.235919047297095	37.0	35.0	37.0	30.0	37.0
5	33.86680602703455	37.0	35.0	37.0	29.0	37.0
6	33.81663943924617	37.0	35.0	37.0	29.0	37.0
7	33.647237582959775	37.0	35.0	37.0	28.0	37.0
8	33.6133665670337	37.0	35.0	37.0	28.0	37.0
9	35.09791376114188	39.0	35.0	39.0	28.0	39.0
10	34.9628974639977	39.0	35.0	39.0	27.0	39.0
11	34.90853434192714	39.0	35.0	39.0	27.0	39.0
12	34.8363367353196	39.0	35.0	39.0	27.0	39.0
13	34.80512137559532	39.0	35.0	39.0	27.0	39.0
14	35.97543439397108	40.0	36.0	41.0	27.0	41.0
15	35.97421309085992	40.0	36.0	41.0	27.0	41.0
16	35.91848872879131	40.0	36.0	41.0	27.0	41.0
17	35.8822317103275	40.0	36.0	41.0	27.0	41.0
18	35.87816607206592	40.0	36.0	41.0	27.0	41.0
19	35.91655909346054	40.0	36.0	41.0	27.0	41.0
20	35.90846987661969	40.0	36.0	41.0	27.0	41.0
21	35.84901085061187	40.0	36.0	41.0	27.0	41.0
22	35.85118380317012	40.0	36.0	41.0	27.0	41.0
23	35.78043116284408	40.0	36.0	41.0	27.0	41.0
24	35.764658781983044	40.0	36.0	41.0	27.0	41.0
25	35.74338129971494	40.0	36.0	41.0	27.0	41.0
26	35.6571943283108	40.0	36.0	41.0	26.0	41.0
27	35.60262169399105	40.0	36.0	41.0	26.0	41.0
28	35.53827902786316	40.0	35.0	41.0	26.0	41.0
29	35.4636085477853	40.0	35.0	41.0	25.0	41.0
30	35.339433428563794	40.0	35.0	41.0	25.0	41.0
31	35.26077396104723	40.0	35.0	41.0	25.0	41.0
32	35.185221799965724	39.0	35.0	41.0	24.0	41.0
33	35.09136660162484	39.0	35.0	41.0	24.0	41.0
34	35.0614602021066	39.0	35.0	41.0	24.0	41.0
35	34.99760613583941	39.0	35.0	41.0	24.0	41.0
36	34.8898162109808	39.0	35.0	41.0	24.0	41.0
37	34.8034767240932	39.0	35.0	41.0	23.0	41.0
38	34.723110891335075	39.0	35.0	41.0	23.0	41.0
39	34.63823405938041	39.0	34.0	41.0	22.0	41.0
40	34.544365103199524	39.0	34.0	41.0	21.0	41.0
41	34.490420219465065	39.0	34.0	41.0	21.0	41.0
42	34.385011187089326	39.0	34.0	41.0	19.0	41.0
43	34.48845442066915	39.0	34.0	41.0	20.0	41.0
44	34.42515302648895	39.0	34.0	41.0	18.0	41.0
45	34.358438508744484	39.0	34.0	41.0	18.0	41.0
46	34.21110894479725	39.0	34.0	41.0	15.0	41.0
47	34.0658646763134	39.0	34.0	41.0	9.0	41.0
48	33.91595689314954	39.0	34.0	41.0	2.0	41.0
49	33.76285768418603	39.0	34.0	41.0	2.0	41.0
50	33.62207370743128	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6254439148527524
1101	2	0.8709995099344106
1101	3	0.7205953460556422
1101	4	0.66696977249741
1101	5	0.7575593104426659
1101	6	0.7434766418708776
1101	7	0.8671187830214393
1101	8	0.7962324403284455
1101	9	0.9260935222520175
1101	10	0.8905936018166329
1101	11	0.9616880179163303
1101	12	1.0287326465523208
1101	13	1.035762595925931
1101	14	1.0735573175297262
1101	15	1.0734320099097303
1101	16	1.1134990292478761
1101	17	1.1887229128558374
1101	18	1.1315405059948134
1101	19	1.135794956068807
1101	20	1.0898751595520366
1101	21	1.1712396871620072
1101	22	1.2038908226250555
1101	23	1.1352636943216652
1101	24	1.1686815281116694
1101	25	1.1350161343074987
1101	26	1.1326354606860463
1101	27	1.0689865265658582
1101	28	1.191105689494762
1101	29	1.2520877976502547
1101	30	1.1991931251328012
1101	31	1.2367599070386603
1101	32	1.1791496131975023
1101	33	1.2681447957133614
1101	34	1.2514872961124937
1101	35	1.2583051350410912
1101	36	1.2260479490010496
1101	37	1.2864343234021547
1101	38	1.3506172792835116
1101	39	1.364600302816136
1101	40	1.38801802075794
1101	41	1.4373857642046701
1101	42	1.4167326011758377
1101	43	1.336795940899016
1101	44	1.4070626058007747
1101	45	1.3805465111762985
1101	46	1.399477422124228
1101	47	1.464014737097088
1101	48	1.5251002566738165
1101	49	1.497346233155639
1101	50	1.529975876000755
1102	1	0.6023375117272671
1102	2	0.6878416418278803
1102	3	0.6060963164452069
1102	4	0.6411720110795187
1102	5	0.5615674349705699
1102	6	0.6416003385310773
1102	7	0.669275830724132
1102	8	0.6588727673334631
1102	9	0.7691663141188201
1102	10	0.6877291274714139
1102	11	0.7893758885888786
1102	12	0.7874447209452384
1102	13	0.7985571733095256
1102	14	0.9004225436106168
1102	15	0.8554992107424155
1102	16	0.9055285348732411
1102	17	0.9430540307373931
1102	18	0.9673784924997122
1102	19	0.9892524703535912
1102	20	0.9190494429636189
1102	21	1.0331147617031036
1102	22	1.047918174238582
1102	23	1.0178157646667714
1102	24	0.9346694641996791
1102	25	1.0552506902955443
1102	26	0.9814648853552654
1102	27	0.9453077200908098
1102	28	1.0170021162204108
1102	29	0.9312029903005978
1102	30	0.9803072118643072
1102	31	1.023340124102333
1102	32	1.0122843871166154
1102	33	0.9437378175823596
1102	34	1.060083104463267
1102	35	0.9736673596004408
1102	36	0.9020706167230372
1102	37	0.857534278948556
1102	38	0.9685089498353534
1102	39	0.9193517421369748
1102	40	0.8106446864126582
1102	41	0.8541159308514565
1102	42	0.8260401008234624
1102	43	0.8158368996511314
1102	44	0.8941167054708004
1102	45	0.7597227823504937
1102	46	0.9800877013868075
1102	47	0.9989344495581918
1102	48	1.03827370161099
1102	49	0.9904067008942548
1102	50	0.989225441403569
1103	1	0.39089910019527707
1103	2	0.6310400272351586
1103	3	0.5282162010253835
1103	4	0.46246557679904754
1103	5	0.3873899261329612
1103	6	0.5271175060700557
1103	7	0.4580555533810653
1103	8	0.4742618631222868
1103	9	0.5542864360341326
1103	10	0.5483128291500705
1103	11	0.5524509667934794
1103	12	0.5650628397876858
1103	13	0.692243237811148
1103	14	0.6736740528301013
1103	15	0.7087904780585177
1103	16	0.7761024397857241
1103	17	0.6732294624464359
1103	18	0.709272141375763
1103	19	0.6907748771103286
1103	20	0.6337751781225833
1103	21	0.6495162256861562
1103	22	0.6095636026820372
1103	23	0.7792849583875636
1103	24	0.7223383870372118
1103	25	0.5882820667144273
1103	26	0.6737345765876057
1103	27	0.7459566978544743
1103	28	0.6996217250992416
1103	29	0.7627375850196216
1103	30	0.820535339111629
1103	31	0.7917750505552092
1103	32	0.8317582169027062
1103	33	0.8765286468099163
1103	34	0.9100865841889529
1103	35	0.7953973364557996
1103	36	0.727840715707849
1103	37	0.8700393780850462
1103	38	0.7455167069469297
1103	39	0.8053464351091719
1103	40	0.7499469924893276
1103	41	0.6153614942012027
1103	42	0.6241607634346167
1103	43	0.6550888478514025
1103	44	0.737148813524314
1103	45	0.6539816632350224
1103	46	0.6755003522418406
1103	47	0.7239327632295911
1103	48	0.7563695243341328
1103	49	0.8181884025336501
1103	50	0.7956479086539971
1104	1	0.5363838150326217
1104	2	0.7066839368187097
1104	3	0.5628456796567534
1104	4	0.5300240086809609
1104	5	0.5733087476376326
1104	6	0.6052267722884679
1104	7	0.6890036331614908
1104	8	0.6138909750252779
1104	9	0.6694397703985047
1104	10	0.7140142163945313
1104	11	0.6717489898054652
1104	12	0.7142404643837352
1104	13	0.7713761575598284
1104	14	0.8744079389963701
1104	15	0.7769779235643739
1104	16	0.8764993029179138
1104	17	0.9053969227447141
1104	18	0.879203544815141
1104	19	0.875598292374498
1104	20	0.8878167568789053
1104	21	0.8771901378759352
1104	22	0.897211422095701
1104	23	0.8254838689514798
1104	24	0.7996965353574055
1104	25	0.735935292514732
1104	26	0.7224955298708409
1104	27	0.8474339379432649
1104	28	0.919941884861224
1104	29	0.8623322168364496
1104	30	0.9584639256337937
1104	31	0.8259619617942704
1104	32	0.825160153491737
1104	33	0.8578343646767337
1104	34	0.8283994480300834
1104	35	0.8531933827013916
1104	36	0.8541720972889664
1104	37	0.8882107519534443
1104	38	0.8800830279562248
1104	39	0.9558323692577773
1104	40	0.9890159415108997
1104	41	0.8879288470366333
1104	42	0.9942221113092771
1104	43	0.8979374886258213
1104	44	0.8924770167235607
1104	45	0.9037639983410912
1104	46	0.8560750512804276
1104	47	0.9070986631834543
1104	48	0.8677175409777149
1104	49	0.8875349729030049
1104	50	0.8498290311946732
1105	1	0.48990979418609015
1105	2	0.604787730996005
1105	3	0.5095023259757241
1105	4	0.48214564846669106
1105	5	0.6238850755615104
1105	6	0.5903432973475091
1105	7	0.4994079336031305
1105	8	0.5647810909916657
1105	9	0.4761098915582238
1105	10	0.6301737245321704
1105	11	0.6547168074600407
1105	12	0.5902863819180695
1105	13	0.6635337334355711
1105	14	0.6843875972087972
1105	15	0.7644085408485637
1105	16	0.7987351693499676
1105	17	0.7024990616421931
1105	18	0.6895364011957383
1105	19	0.665637899516696
1105	20	0.7043297290547841
1105	21	0.8265621357170758
1105	22	0.8441135551167704
1105	23	0.872224305247002
1105	24	0.797565961655458
1105	25	0.7349896812831815
1105	26	0.6870402556596034
1105	27	0.8062452096618671
1105	28	0.753516012403459
1105	29	0.7337165457316388
1105	30	0.873422791783824
1105	31	0.7794833734362001
1105	32	0.9065815685354437
1105	33	0.7748300682845013
1105	34	0.6642796235234201
1105	35	0.7977283858260478
1105	36	0.8524126509422629
1105	37	0.8420281656116302
1105	38	0.7808276618630217
1105	39	0.758120678902884
1105	40	0.9030051317790466
1105	41	0.8666219510963415
1105	42	0.8674650765404905
1105	43	0.8983992844936424
1105	44	0.825125173333852
1105	45	0.7683122622470009
1105	46	0.859750602802329
1105	47	0.7737533679691637
1105	48	0.717246668032864
1105	49	0.8894990710352388
1105	50	0.9405156093029703
1106	1	0.344681342393244
1106	2	0.5117962107488907
1106	3	0.35169259935692665
1106	4	0.3244657643061615
1106	5	0.367824533576659
1106	6	0.3169436529347962
1106	7	0.25437650186506744
1106	8	0.286203691442914
1106	9	0.31146884380390105
1106	10	0.35764136288700854
1106	11	0.3554781085165999
1106	12	0.3559114220293864
1106	13	0.318546848064301
1106	14	0.3470513510447901
1106	15	0.46743519422202695
1106	16	0.5714139246512531
1106	17	0.5273286822603609
1106	18	0.48612913782753964
1106	19	0.3065059551238605
1106	20	0.3842120655759018
1106	21	0.35567542251880013
1106	22	0.2873915867321273
1106	23	0.39116630674215
1106	24	0.4036937285259583
1106	25	0.400557866340975
1106	26	0.3792759333676585
1106	27	0.32013477223330256
1106	28	0.3180656164785418
1106	29	0.3236758865318805
1106	30	0.49130359802848034
1106	31	0.4003871039681073
1106	32	0.2603277531465551
1106	33	0.4400000788110674
1106	34	0.3994222887688892
1106	35	0.4016590994042559
1106	36	0.42341289860222986
1106	37	0.41832932283085
1106	38	0.34398649242698554
1106	39	0.378794178894843
1106	40	0.5013726162009888
1106	41	0.44529817142966976
1106	42	0.5355496516752964
1106	43	0.55717918153033
1106	44	0.35868046386006824
1106	45	0.34992412463237343
1106	46	0.42577691150554386
1106	47	0.447380500151894
1106	48	0.34493348478083874
1106	49	0.3598336497842851
1106	50	0.38532249870867474
1107	1	0.49077139006877957
1107	2	0.6103766350683095
1107	3	0.456589231797512
1107	4	0.5076768150176107
1107	5	0.46826453403179613
1107	6	0.42750871798886436
1107	7	0.5160303087237068
1107	8	0.469291035026167
1107	9	0.48568711398397113
1107	10	0.487909864247257
1107	11	0.6396577265036996
1107	12	0.5732102034546358
1107	13	0.5929370645372245
1107	14	0.643305181665788
1107	15	0.5932507681953183
1107	16	0.6171396515265499
1107	17	0.6048983439273243
1107	18	0.6708523262590305
1107	19	0.618910776711715
1107	20	0.6233020891715526
1107	21	0.5967118157005373
1107	22	0.755387824976971
1107	23	0.7084712536777431
1107	24	0.6712991051685151
1107	25	0.7249527532541364
1107	26	0.7705890997488467
1107	27	0.7015696267365215
1107	28	0.6129701794060409
1107	29	0.7119072238892912
1107	30	0.780554546736461
1107	31	0.7177050169984227
1107	32	0.6332356803787178
1107	33	0.7311193359226351
1107	34	0.7937650228737141
1107	35	0.6296570258875605
1107	36	0.6342925369910759
1107	37	0.6436240831790485
1107	38	0.6734945191011548
1107	39	0.614878317735787
1107	40	0.6716826672794625
1107	41	0.6494480097371778
1107	42	0.5875550699152043
1107	43	0.6454577789799245
1107	44	0.7670874415242466
1107	45	0.7553692758024795
1107	46	0.7709507590512601
1107	47	0.8151697307253087
1107	48	0.8198759775046085
1107	49	0.8389017421568639
1107	50	0.7585176646310288
1108	1	0.2814176770207695
1108	2	0.47546894710881205
1108	3	0.3478814931184786
1108	4	0.32168652392435604
1108	5	0.4261599296317513
1108	6	0.3301223043112813
1108	7	0.3108121081267683
1108	8	0.34792048016254995
1108	9	0.3950086139795914
1108	10	0.3278947634797831
1108	11	0.27686930818811106
1108	12	0.4098017460047032
1108	13	0.3343779466543211
1108	14	0.2944614968431054
1108	15	0.3105070113215973
1108	16	0.27862768528656545
1108	17	0.32043786547129116
1108	18	0.3297075348009457
1108	19	0.38044211433550146
1108	20	0.3087902856316731
1108	21	0.36730904150670796
1108	22	0.3453551659379386
1108	23	0.26831769040800424
1108	24	0.330887510509946
1108	25	0.3231465204535624
1108	26	0.38734722419324186
1108	27	0.3518223939135794
1108	28	0.3079527251108445
1108	29	0.4096265711688858
1108	30	0.47213484218364243
1108	31	0.4591106517845418
1108	32	0.31203466583104955
1108	33	0.38340428502457513
1108	34	0.29941172376931036
1108	35	0.2932509405372272
1108	36	0.4225686207680326
1108	37	0.40590365820740715
1108	38	0.25691357298431683
1108	39	0.3609599458459698
1108	40	0.36921191438993617
1108	41	0.40088697058046563
1108	42	0.2876514213390706
1108	43	0.29178566757062896
1108	44	0.3939954240180299
1108	45	0.35281402087011315
1108	46	0.4408962558221745
1108	47	0.39850789231084605
1108	48	0.34873504036504954
1108	49	0.3653157911806062
1108	50	0.3743807422867178
1109	1	0.32860560810413375
1109	2	0.3652088493501431
1109	3	0.3206815890587116
1109	4	0.33504294287686065
1109	5	0.3838215428159728
1109	6	0.3742363024283222
1109	7	0.44285396335803284
1109	8	0.2991089952299575
1109	9	0.31431309941371666
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2211	3	-0.402429132780874
2211	4	-0.37337265250319973
2211	5	-0.4158218566619212
2211	6	-0.5233459801562645
2211	7	-0.4604085238215987
2211	8	-0.5256615020328184
2211	9	-0.6734895576172164
2211	10	-0.6782700290362627
2211	11	-0.7226482605299367
2211	12	-0.5883243348414808
2211	13	-0.5283052677041269
2211	14	-0.7071558032108456
2211	15	-0.635089739508139
2211	16	-0.6100480452635111
2211	17	-0.6765157217607083
2211	18	-0.7504519389764965
2211	19	-0.6766653256740227
2211	20	-0.7791784261555961
2211	21	-0.7281583139555039
2211	22	-0.7911022455814987
2211	23	-0.729629483050978
2211	24	-0.7286226796987805
2211	25	-0.5774893646310773
2211	26	-0.7190853107400983
2211	27	-0.5766606721928937
2211	28	-0.615802362923084
2211	29	-0.5686368125745815
2211	30	-0.6217732918400856
2211	31	-0.6015804913149765
2211	32	-0.6502312789268458
2211	33	-0.49723791042789145
2211	34	-0.48223231517416565
2211	35	-0.5538363348295263
2211	36	-0.7222788149158959
2211	37	-0.5265987006050565
2211	38	-0.5933113995830084
2211	39	-0.6590084290853255
2211	40	-0.6049364100609509
2211	41	-0.6174044474581706
2211	42	-0.798307139063759
2211	43	-0.7120492886601895
2211	44	-0.6260735784274587
2211	45	-0.6435736786346666
2211	46	-0.6718950036997171
2211	47	-0.6430001366158393
2211	48	-0.5312778859146263
2211	49	-0.6424072758399717
2211	50	-0.689827124108227
2212	1	-1.0333689573493317
2212	2	-2.450901122673347
2212	3	-1.389194900140371
2212	4	-0.8649658716845394
2212	5	-0.7656311604188062
2212	6	-0.7429263291460231
2212	7	-0.883983945607639
2212	8	-0.7039614535316758
2212	9	-0.8032719829781243
2212	10	-0.9139121280270501
2212	11	-0.8889919838381459
2212	12	-0.8599213105699448
2212	13	-2.3811130292929548
2212	14	-1.747333468303374
2212	15	-1.3626254433415639
2212	16	-1.111378154227232
2212	17	-0.9406965929758044
2212	18	-0.9593984472270307
2212	19	-0.8497339212037573
2212	20	-0.9623256040809594
2212	21	-0.9342246353429218
2212	22	-1.0012848863412103
2212	23	-0.8539645384280377
2212	24	-0.8624538768935253
2212	25	-0.9332329330821594
2212	26	-1.0317911784993399
2212	27	-0.9944423859191431
2212	28	-1.0456738305038442
2212	29	-0.9192386071544831
2212	30	-0.9208376956558411
2212	31	-0.8478848060332709
2212	32	-0.841751024465978
2212	33	-0.8305941153410075
2212	34	-0.9548424160924469
2212	35	-1.0422781725695387
2212	36	-1.0014239141850396
2212	37	-0.9830670742323377
2212	38	-0.9987691402484202
2212	39	-0.9407456337547444
2212	40	-0.8764154914797118
2212	41	-0.848937258047215
2212	42	-0.950313395120908
2212	43	-0.9965052817527607
2212	44	-1.1234264180167486
2212	45	-1.1157286497631205
2212	46	-0.9999473457599493
2212	47	-1.0428539426496926
2212	48	-1.0207087049938153
2212	49	-0.9256750349102063
2212	50	-0.959333453300026
2213	1	-0.9364713521185735
2213	2	-2.0852409390064395
2213	3	-1.2150605128308953
2213	4	-0.9074488220923342
2213	5	-0.8858971755300971
2213	6	-0.8065336941952594
2213	7	-0.8197683115786418
2213	8	-0.7568870980002416
2213	9	-0.8885045589036054
2213	10	-0.876285518667288
2213	11	-0.8191775103364236
2213	12	-0.7600180360404067
2213	13	-0.8533973245816782
2213	14	-0.9112958501669652
2213	15	-0.8714727691654787
2213	16	-0.9881519562071333
2213	17	-0.9439172077644997
2213	18	-0.8843552686024196
2213	19	-1.0581026142593686
2213	20	-1.119255433297404
2213	21	-1.0126926715661426
2213	22	-1.0404999517903306
2213	23	-0.9607982441997649
2213	24	-0.9625110024480037
2213	25	-0.9422205086085214
2213	26	-0.956691810804756
2213	27	-1.0245075518619657
2213	28	-0.874357369648223
2213	29	-0.9389422410265027
2213	30	-0.9803918666017282
2213	31	-1.073421043746933
2213	32	-0.9270090192564453
2213	33	-1.013801253671808
2213	34	-1.0945694709958573
2213	35	-0.9571370153690211
2213	36	-1.0720687372764317
2213	37	-0.988337252240207
2213	38	-0.9165431255117227
2213	39	-1.0241699027059568
2213	40	-0.9130231165148857
2213	41	-0.9623575714662778
2213	42	-0.9661823479394869
2213	43	-1.1294579861639136
2213	44	-1.0144245276558905
2213	45	-0.9966907671813274
2213	46	-1.0034163519135433
2213	47	-0.9803984535349883
2213	48	-1.1376568893064487
2213	49	-1.2003481683739636
2213	50	-1.0881843556028414
2214	1	-0.9780541748704543
2214	2	-1.875969749679836
2214	3	-1.0635292889398222
2214	4	-0.6995758506111187
2214	5	-0.6923996506346839
2214	6	-0.7479834613324456
2214	7	-0.8069404714416777
2214	8	-0.8968416035629971
2214	9	-0.9218873042782576
2214	10	-1.0376240331187603
2214	11	-0.9090062841056934
2214	12	-0.914517885970227
2214	13	-0.830644281602126
2214	14	-1.0257726901624125
2214	15	-1.112790075516088
2214	16	-1.1061960919578198
2214	17	-0.9725084887243725
2214	18	-1.1025343188056667
2214	19	-0.9747075262012572
2214	20	-1.0298214766916018
2214	21	-1.169785786207612
2214	22	-1.1277156332440157
2214	23	-1.0988028907987513
2214	24	-1.1017099523780942
2214	25	-1.1049031487233805
2214	26	-1.091929577235085
2214	27	-1.1552753708676633
2214	28	-1.1505236726848977
2214	29	-1.1405357483784542
2214	30	-1.0750218327039605
2214	31	-1.2666991638124898
2214	32	-1.136130448212036
2214	33	-1.1256586193416567
2214	34	-1.1397549074224855
2214	35	-1.226555117327024
2214	36	-1.2519350942689655
2214	37	-1.2026530177381431
2214	38	-1.2554020196419842
2214	39	-1.2829631972085807
2214	40	-1.1848505201967257
2214	41	-1.300316911990258
2214	42	-1.2176879446524609
2214	43	-1.0882126044466034
2214	44	-1.099545484714504
2214	45	-1.1841828206494327
2214	46	-1.193577138910804
2214	47	-1.1777409845633287
2214	48	-1.1160191490485616
2214	49	-1.1386184552967435
2214	50	-1.2818529555435063
2215	1	-0.5061172886634431
2215	2	-0.3436106063367248
2215	3	-0.6111983238799645
2215	4	-0.5842418591665677
2215	5	-0.5795101716085043
2215	6	-0.7267350428849397
2215	7	-0.8659987698997469
2215	8	-0.7142236185883277
2215	9	-0.8270734060815812
2215	10	-0.9690289151917213
2215	11	-0.7971259861945015
2215	12	-0.8924676841340045
2215	13	-0.8884211170140688
2215	14	-0.956023560305951
2215	15	-1.1410499274874084
2215	16	-0.9682506503049382
2215	17	-1.0332074306483747
2215	18	-1.018222856083959
2215	19	-0.9551020685127156
2215	20	-1.0564091700117757
2215	21	-1.025844812295702
2215	22	-1.0690065508457067
2215	23	-1.0616520489261916
2215	24	-1.0453696069160543
2215	25	-1.1111415332920629
2215	26	-1.143234676670133
2215	27	-1.0900220212205909
2215	28	-1.2332338859222034
2215	29	-1.1100668209607107
2215	30	-0.9574247728853535
2215	31	-0.965882549748379
2215	32	-1.0262460347884215
2215	33	-1.1417598665087638
2215	34	-1.0660204970113512
2215	35	-1.1667170497640242
2215	36	-1.1197543824892406
2215	37	-1.097845561616083
2215	38	-1.1355761873861923
2215	39	-1.2293771862431555
2215	40	-1.138011771349575
2215	41	-1.2213285030899996
2215	42	-1.2595785776905757
2215	43	-1.1423450331087395
2215	44	-1.081115358051271
2215	45	-1.0300881638637094
2215	46	-0.9492802287893269
2215	47	-1.1115410207130978
2215	48	-1.0785316044986004
2215	49	-1.0790081776386913
2215	50	-1.1528724254395541
2216	1	-1.126945095322263
2216	2	-0.7460742329810763
2216	3	-0.7834359865587786
2216	4	-2.2096927763513037
2216	5	-1.422790762360009
2216	6	-1.2217699541716769
2216	7	-1.0258329534491253
2216	8	-1.1088425564074313
2216	9	-1.1739917107518778
2216	10	-1.2067947766124547
2216	11	-1.360892243265667
2216	12	-1.249701655156393
2216	13	-1.3030320229130439
2216	14	-1.344128481181265
2216	15	-1.4719806032605192
2216	16	-1.5290201255492235
2216	17	-1.420037946323589
2216	18	-1.497699894845816
2216	19	-1.4975901692716889
2216	20	-1.5515315892304429
2216	21	-1.5042078140069393
2216	22	-1.6342565511159606
2216	23	-1.4786068053255121
2216	24	-1.6661229858897144
2216	25	-1.7475625075218204
2216	26	-1.9097197873495588
2216	27	-2.043263814446753
2216	28	-1.74881905400337
2216	29	-1.750531375113468
2216	30	-1.8581649418907915
2216	31	-1.6834436999020426
2216	32	-1.68488229474589
2216	33	-1.6470093695134338
2216	34	-1.7003014996284875
2216	35	-1.8232770097233342
2216	36	-1.950976499132615
2216	37	-1.9485420577314656
2216	38	-2.008672876354133
2216	39	-2.0179695354879
2216	40	-2.0109553889999106
2216	41	-2.165356397526999
2216	42	-2.1537213296663964
2216	43	-1.7408851262271199
2216	44	-1.648430415793534
2216	45	-1.6406268970688345
2216	46	-1.6747193088912482
2216	47	-1.6012258948670706
2216	48	-1.685683851003958
2216	49	-1.6211306800952912
2216	50	-1.621590636926605
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61188.0
3	9742.0
4	17063.0
5	10053.0
6	7478.0
7	5760.0
8	4874.0
9	4331.0
10	4127.0
11	4099.0
12	4250.0
13	4538.0
14	4830.0
15	5240.0
16	5647.0
17	6160.0
18	6619.0
19	7483.0
20	8748.0
21	10874.0
22	13379.0
23	16177.0
24	18745.0
25	21497.0
26	23683.0
27	26846.0
28	31841.0
29	36731.0
30	42432.0
31	50830.0
32	62447.0
33	77228.0
34	98187.0
35	125500.0
36	164520.0
37	223883.0
38	357140.0
39	959834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.545290712577234	18.14065981761369	14.127744325785537	26.186305144023535
2	14.736659631783	20.04100646661601	38.28251615867836	26.939817742922628
3	17.50760712375054	24.79574464906995	29.651332136004875	28.045316091174637
4	12.321242415332389	16.64302886694225	36.6435556072687	34.39217311045666
5	13.860710239594873	37.521259331003016	34.26015168692423	14.357878742477883
6	32.44327169311452	36.555437467832235	17.346431240900134	13.654859598153113
7	28.123693828480878	31.711907959020376	21.85426713824824	18.310131074250506
8	26.765107616382256	34.15553124850101	19.905472468591082	19.17388866652565
9	26.677969980422354	15.095861249202287	18.847895590095497	39.37827318027986
10	14.502158118927158	28.643941637008997	33.754301197670486	23.09959904639336
11	35.07326551084521	22.57831366045998	23.547283122251535	18.801137706443267
12	23.552122185673305	24.38845228989998	30.1573132679123	21.902112256514414
13	28.19669797557732	21.491825157704582	26.076209396133613	24.23526747058449
14	22.494646912512167	20.406382413952436	26.995212206415843	30.103758467119558
15	23.765937467558818	28.479424819823645	23.758820069019105	23.99581764359843
16	23.82128764694825	27.45827405067124	25.123107378892307	23.5973309234882
17	22.62793615088463	27.43274331758997	26.4858211391066	23.453499392418802
18	22.78245549180231	26.782274588149235	27.436555909060523	22.998714010987932
19	23.553707144893764	26.50782133341199	27.45703920007079	22.481432321623455
20	23.83255211805101	25.92057756053976	27.361057529141707	22.885812792267522
21	23.99722354949141	25.748275041636926	27.276502616195042	22.977998792676622
22	23.737109976255063	25.59356440666368	27.246664973034278	23.422660644046974
23	23.615208044701877	25.560710230992996	27.186576223676123	23.637505500629008
24	23.280820686897034	25.871991625451297	27.184368662608637	23.66281902504303
25	23.459394713475533	25.67048919501973	27.27249848013798	23.597617611366758
26	23.303237974342064	25.95904624492266	27.34521024477008	23.392505535965196
27	23.33461008180366	26.111380705158403	27.117233037007093	23.436776176030843
28	23.395465000454198	25.845509889564532	27.237917790205245	23.52110731977602
29	23.54905052713538	25.99510812075549	27.10167951899142	23.354161833117708
30	23.48669099700242	26.035477829207647	27.08791362193395	23.389917551855977
31	23.62477286481126	25.574566993133153	27.16892723770187	23.631732904353722
32	23.355072503658803	26.088105373504533	27.009195566813542	23.547626556023125
33	23.560005425818638	25.82375132450917	26.999188093080313	23.617055156591878
34	23.707560181231727	25.850290389088354	27.068304734765388	23.373844694914535
35	23.472825950203188	25.900153177409006	27.064985594403833	23.562035277983973
36	23.608055295625576	25.967909350402213	26.872006982724052	23.552028371248163
37	23.579513510750807	25.768321977723872	27.079688464456876	23.572476047068445
38	23.628066580460303	25.80832228232729	27.052823481261516	23.510787655950892
39	23.68169136011656	25.99167261938225	26.82189461865338	23.504741401847813
40	23.60686606533887	25.739804191735644	27.20323608438507	23.45009365854042
41	23.385640370056755	25.887855537289106	26.87951141312338	23.846992679530754
42	23.691202641043493	25.877304534910373	26.791787071765043	23.639705752281095
43	23.52388076657137	25.75426963805513	26.92563653938303	23.796213055990464
44	23.628212969965894	25.979359316453266	26.648408668866956	23.74401904471388
45	23.62189929306398	26.026951935897756	26.57779126518906	23.773357505849205
46	23.74649368863955	25.768715526512747	26.49049679557733	23.994293989270375
47	23.579095084699457	25.988926865029217	26.52147508151549	23.910502968755836
48	23.55005326278798	26.18064536417203	26.423334997385993	23.845966375653994
49	23.697680817610063	25.930070754716983	26.525157232704405	23.847091194968552
50	23.760268773690555	25.982164301165213	26.434837435077608	23.822729490066624
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	541.0
1	409.5
2	278.0
3	414.0
4	550.0
5	586.5
6	623.0
7	602.0
8	581.0
9	753.0
10	925.0
11	1342.0
12	1759.0
13	3350.5
14	4942.0
15	6792.5
16	8643.0
17	9877.0
18	11111.0
19	11966.5
20	12822.0
21	14053.5
22	15285.0
23	18215.0
24	21145.0
25	25649.5
26	30154.0
27	35309.5
28	40465.0
29	46150.0
30	51835.0
31	58366.5
32	64898.0
33	73375.5
34	81853.0
35	89917.5
36	97982.0
37	104820.0
38	111658.0
39	119428.5
40	127199.0
41	135207.5
42	143216.0
43	150092.0
44	156968.0
45	164157.0
46	171346.0
47	175875.0
48	180404.0
49	181500.5
50	182597.0
51	183682.0
52	184767.0
53	181014.5
54	177262.0
55	169001.5
56	160741.0
57	150227.0
58	139713.0
59	126723.5
60	113734.0
61	99739.5
62	85745.0
63	73355.0
64	60965.0
65	50926.5
66	40888.0
67	33253.0
68	25618.0
69	20352.0
70	15086.0
71	11791.0
72	8496.0
73	6994.0
74	5492.0
75	4124.0
76	2756.0
77	2122.0
78	1488.0
79	1125.0
80	762.0
81	554.0
82	346.0
83	264.5
84	183.0
85	146.5
86	110.0
87	73.0
88	36.0
89	29.0
90	22.0
91	14.5
92	7.0
93	6.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015408780803803767
2	5.896217144312666E-4
3	2.7515680006792443E-4
4	0.0022012544005433955
5	0.039426038638304024
6	0.02739775566390619
7	0.031014102179084624
8	0.024292414634568184
9	0.031328567093447966
10	0.032114729379356326
11	0.03903295749534985
12	0.03887572503816818
13	0.03765717349501023
14	0.04241345532475578
15	0.03694962743769271
16	0.043317541953550386
17	0.044575401611003755
18	0.04772005075463718
19	0.05027507818383933
20	0.046029801839934215
21	0.04673734789725174
22	0.04504709898254877
23	0.04374993121079998
24	0.04170590926743826
25	0.03970119543837195
26	0.04272792023911912
27	0.04268861212482371
28	0.04209899041039244
29	0.039858427895553626
30	0.03738201669494231
31	0.035966924580307265
32	0.03231126995083341
33	0.023938641605909427
34	0.020440219433617245
35	0.02154084663388894
36	0.02295593874852398
37	0.018632046176028025
38	0.0194575165762318
39	0.013639915660509967
40	0.007429233601833959
41	0.006996844344584363
42	0.006328606401562261
43	0.002083330057657142
44	0.0037735789723601066
45	0.0026729517720884086
46	7.861622859083554E-4
47	3.5377302865876E-4
48	5.503136001358489E-4
49	1.572324571816711E-4
50	1.9654057147708886E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2544004.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.51633902237589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86373642071321	47.89892007205511
2	12.416605131720901	15.276481815798304
3	4.20366680047898	7.757825761061134
4	1.9042838850973118	4.685782922819733
5	1.0212056218334717	3.1410415622132017
6	0.5905360618534514	2.1796569951549394
7	0.4088544188759501	1.7605858929658598
8	0.28320356627652765	1.393731727633022
9	0.21407150336088887	1.1852005658200306
>10	1.0318786887287832	11.03674807769012
>50	0.047888410330316634	1.9900916015655246
>100	0.013683659444787787	1.3555916043682028
>500	1.2861042845709636E-4	0.04727206524443682
>1k	2.5722085690227885E-4	0.29106933561042575
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2751	0.10813662242669429	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	3.930811429541777E-5	0.0	0.0	0.0	0.0
6	3.930811429541777E-5	0.0	0.0	0.0	0.0
7	3.930811429541777E-5	0.0	0.0	0.0	0.0
8	7.861622859083554E-5	0.0	0.0	0.0	0.0
9	7.861622859083554E-5	0.0	0.0	0.0	0.0
10	1.1792434288625332E-4	0.0	0.0	0.0	0.0
11	1.1792434288625332E-4	0.0	0.0	0.0	0.0
12	1.1792434288625332E-4	0.0	0.0	0.0	3.930811429541777E-5
13	1.1792434288625332E-4	0.0	0.0	0.0	1.1792434288625332E-4
14	1.1792434288625332E-4	0.0	0.0	0.0	1.1792434288625332E-4
15	1.1792434288625332E-4	0.0	0.0	0.0	1.1792434288625332E-4
16	1.1792434288625332E-4	0.0	0.0	0.0	1.1792434288625332E-4
17	1.1792434288625332E-4	0.0	0.0	0.0	1.1792434288625332E-4
18	1.1792434288625332E-4	0.0	0.0	0.0	1.9654057147708886E-4
19	1.1792434288625332E-4	0.0	0.0	0.0	1.9654057147708886E-4
20	1.1792434288625332E-4	0.0	0.0	0.0	2.7515680006792443E-4
21	1.1792434288625332E-4	0.0	0.0	0.0	2.7515680006792443E-4
22	1.1792434288625332E-4	0.0	0.0	7.861622859083554E-5	2.7515680006792443E-4
23	1.1792434288625332E-4	0.0	0.0	4.323892572495955E-4	2.7515680006792443E-4
24	1.572324571816711E-4	0.0	0.0	7.0754605731752E-4	3.144649143633422E-4
25	1.572324571816711E-4	0.0	0.0	8.254704002037732E-4	3.144649143633422E-4
26	1.572324571816711E-4	0.0	0.0	0.0012578596574533687	3.5377302865876E-4
27	1.572324571816711E-4	0.0	0.0	0.0016116326861121287	3.5377302865876E-4
28	1.572324571816711E-4	0.0	0.0	0.0025550274292021552	3.5377302865876E-4
29	1.572324571816711E-4	0.0	0.0	0.0035377302865876	3.5377302865876E-4
30	1.572324571816711E-4	0.0	0.0	0.005778292801426413	3.5377302865876E-4
31	1.572324571816711E-4	0.0	0.0	0.01147796937426199	3.5377302865876E-4
32	1.572324571816711E-4	0.0	0.0	0.017924500118710506	3.5377302865876E-4
33	1.572324571816711E-4	0.0	0.0	0.025589582406316973	3.5377302865876E-4
34	1.572324571816711E-4	0.0	0.0	0.035102146065808075	3.5377302865876E-4
35	1.9654057147708886E-4	0.0	0.0	0.04685527224013799	3.5377302865876E-4
36	2.3584868577250663E-4	0.0	0.0	0.06116342584367006	3.5377302865876E-4
37	2.7515680006792443E-4	0.0	0.0	0.07767283384774552	3.5377302865876E-4
38	2.7515680006792443E-4	0.0	0.0	0.09697311796679565	3.5377302865876E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1715	0.0	27.4556	1
GTATCAA	4165	0.0	24.882109	1
CGCTACG	500	0.0	18.921438	28
CGCTATC	490	0.0	18.854126	33
CGCACGA	140	4.096364E-9	18.854126	35
TACGCTA	485	0.0	18.598253	31
CTCGTAT	495	0.0	17.773186	39
ACCGTCG	90	2.2152963E-4	17.11241	8
TATCAAC	6070	0.0	16.963396	2
GACGCTA	545	0.0	16.956083	26
TCTCGTA	545	0.0	16.950085	38
ATCTCCG	625	0.0	16.897947	10
CGAGACG	565	0.0	16.745295	23
ATCAACG	6180	0.0	16.590258	3
ACGCTAT	545	0.0	16.549765	32
TCAACGC	6175	0.0	16.532755	4
ACGCTAC	560	0.0	16.501904	27
CAACGCA	6320	0.0	16.188574	5
TATGCCG	540	0.0	15.882914	43
TATCTCG	570	0.0	15.821718	36
>>END_MODULE