FastQCFastQC Report
Mon 6 Feb 2017
SRR2077458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2994239
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39770.13282172865960268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.026.327871
GTATCAA59300.025.822731
TATCAAC80750.018.6892952
ATCAACG82500.018.186193
TCAACGC82850.018.1368264
CAACGCA83400.018.0163155
AACGCAG86450.017.251726
TGCGATA1854.2200554E-1016.65043813
GGACCGT2252.5465852E-1115.6424656
GACCGTA850.002982832315.5282277
GTACATG103100.015.3005861
ACGCAGA98100.015.0691877
TACATGG104800.014.9251812
GTATAGG5250.014.6675091
AGAGTAC96100.014.42378711
CGCAGAG102300.014.4075028
GAGTACT48250.014.13623412
TACCGAC1100.001019203513.9989327
CGATACT2052.9282091E-813.95248715
GTATCGA950.006239611713.8957659
Produced by FastQC (version 0.11.5)