FastQCFastQC Report
Mon 6 Feb 2017
SRR2077458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2994239
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48390.16161034573392438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29450.027.196261
GTATCAA67600.024.9981351
TAGTCGG1557.2577677E-1018.44720840
TATCAAC96450.017.4519942
ATCAACG97850.017.157613
CGCACGA3100.017.03075235
TCAACGC98800.016.9483764
CAACGCA100050.016.8251615
CGAATCG2550.016.38961237
AACGCAG103250.016.3009786
TAGACGT1105.617093E-516.0028634
CGACTCA2900.015.93202121
TATGCGA1857.572453E-915.46067412
TTAGTCG1857.601557E-915.45628439
TACCGTA2001.2787496E-915.4006967
TATAACG1057.1553E-414.66882
GTATGCG1655.419806E-714.6678211
CGCGCAA1057.1598316E-414.66757521
GTACATG100700.014.661761
TATCGTA1707.594408E-714.23641412
Produced by FastQC (version 0.11.5)