FastQCFastQC Report
Mon 6 Feb 2017
SRR2077457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406596
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48410.14210666600911878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24350.027.6467341
GTATCAA59050.025.8932321
TAGATCG901.0033707E-519.5543485
TCGATCG1252.2982931E-819.35510641
TATCAAC79500.019.2044092
TCAACGC79800.018.938964
ATCAACG80750.018.7973253
CAACGCA81800.018.5832045
AACGCAG83400.018.1726026
TACCGTC2055.456968E-1217.1694187
ACCGTCG1702.4738256E-916.8224898
ATACCGT2800.015.7121596
ACGCAGA96850.015.4908127
GTATTAC4300.015.3487911
GAGTACT51950.015.20392912
CGCAGAG99400.014.9387158
AGTACTT55000.014.64081713
AGAGTAC97550.014.47953611
GTACTTT54600.014.14343814
GTACATG98500.013.9816841
Produced by FastQC (version 0.11.5)