##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077457_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3406596
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.17614827235164	31.0	30.0	34.0	23.0	34.0
2	29.164165636312614	31.0	30.0	34.0	22.0	34.0
3	29.228300626196944	31.0	30.0	34.0	22.0	34.0
4	32.576935744655366	37.0	33.0	37.0	23.0	37.0
5	32.63244482175168	37.0	35.0	37.0	25.0	37.0
6	32.668841565010936	37.0	35.0	37.0	24.0	37.0
7	32.475296747838605	37.0	33.0	37.0	22.0	37.0
8	32.40349516056497	37.0	33.0	37.0	22.0	37.0
9	33.6049284975383	38.0	34.0	39.0	18.0	39.0
10	33.51897876942262	38.0	34.0	39.0	17.0	39.0
11	33.60522498118356	38.0	34.0	39.0	18.0	39.0
12	33.538514106163454	38.0	34.0	39.0	17.0	39.0
13	33.55442911340235	38.0	34.0	39.0	17.0	39.0
14	34.42223938500486	39.0	34.0	41.0	17.0	41.0
15	34.52985238049948	39.0	34.0	41.0	17.0	41.0
16	34.43961919758022	39.0	34.0	41.0	17.0	41.0
17	34.272460837739494	39.0	34.0	41.0	17.0	41.0
18	34.102406038168304	39.0	33.0	41.0	16.0	41.0
19	34.44766417855243	39.0	34.0	41.0	18.0	41.0
20	34.484316308714035	39.0	34.0	41.0	18.0	41.0
21	34.48891855682329	39.0	34.0	41.0	18.0	41.0
22	34.39726137176231	39.0	34.0	41.0	18.0	41.0
23	34.1548352079319	39.0	34.0	41.0	16.0	41.0
24	34.30126994806546	39.0	34.0	41.0	17.0	41.0
25	34.20408143495736	39.0	34.0	41.0	17.0	41.0
26	34.24867639133023	39.0	34.0	41.0	17.0	41.0
27	34.23424615070293	39.0	34.0	41.0	16.0	41.0
28	34.122608903433225	39.0	34.0	41.0	16.0	41.0
29	34.08132810582764	39.0	34.0	41.0	16.0	41.0
30	33.87256780669031	39.0	33.0	41.0	10.0	41.0
31	33.781011895745785	39.0	33.0	41.0	10.0	41.0
32	33.7955880298104	39.0	33.0	41.0	10.0	41.0
33	33.7801104093353	39.0	33.0	41.0	10.0	41.0
34	33.766380281078234	39.0	33.0	41.0	9.0	41.0
35	33.70392761571962	39.0	33.0	41.0	9.0	41.0
36	33.57209219995561	39.0	33.0	41.0	8.0	41.0
37	33.4769323982063	38.0	33.0	41.0	8.0	41.0
38	33.37893075668497	38.0	33.0	41.0	8.0	41.0
39	33.31003852526099	38.0	33.0	41.0	2.0	41.0
40	33.271926286533535	38.0	33.0	41.0	2.0	41.0
41	33.14740432971799	38.0	33.0	40.0	2.0	41.0
42	32.965631381003206	38.0	32.0	40.0	2.0	41.0
43	32.86457096761694	38.0	32.0	40.0	2.0	41.0
44	32.78733668447917	38.0	32.0	40.0	2.0	41.0
45	32.69665995028468	38.0	32.0	40.0	2.0	41.0
46	32.534429383466666	38.0	32.0	40.0	2.0	41.0
47	32.3466422199756	38.0	32.0	40.0	2.0	41.0
48	32.156912941834015	38.0	31.0	40.0	2.0	41.0
49	32.023931807587395	38.0	31.0	40.0	2.0	41.0
50	31.826019287288542	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7782266741462998
1101	2	0.7860998360666223
1101	3	0.863660629902629
1101	4	0.8602759974624306
1101	5	0.7517034540895011
1101	6	0.7916593445365976
1101	7	0.9150194115791805
1101	8	0.8795108925370911
1101	9	1.159458695695676
1101	10	1.1864689359488736
1101	11	1.0983402543395755
1101	12	1.0776663256698598
1101	13	1.052672397122791
1101	14	1.2960407345025402
1101	15	1.2573549955634604
1101	16	1.0557719767626494
1101	17	1.3241055179248349
1101	18	1.5584937754784747
1101	19	1.11802931344468
1101	20	1.2585191769525537
1101	21	1.297614800093669
1101	22	1.202370333711805
1101	23	1.2440269092560499
1101	24	1.1662869737298394
1101	25	1.193611550243368
1101	26	1.20374221831959
1101	27	1.2451422285203648
1101	28	1.3232788836942078
1101	29	1.262783458750988
1101	30	1.5148528352476873
1101	31	1.5494235001300893
1101	32	1.4632883825671357
1101	33	1.368390589241578
1101	34	1.3121119217586994
1101	35	1.2403503845069395
1101	36	1.3140354311671985
1101	37	1.3295753550589566
1101	38	1.3696494246265374
1101	39	1.344782923930211
1101	40	1.4436898301862726
1101	41	1.4799772781597653
1101	42	1.4550333017003538
1101	43	1.3882034088483053
1101	44	1.3697045901563456
1101	45	1.4045154121532661
1101	46	1.368499687755623
1101	47	1.378773381518421
1101	48	1.3544989248474337
1101	49	1.4176829346833628
1101	50	1.5149619134228907
1102	1	0.543171854846392
1102	2	0.5547457165975906
1102	3	0.49855480561895504
1102	4	0.5767467602238199
1102	5	0.4892503844655778
1102	6	0.6000471465678885
1102	7	0.6099907764690187
1102	8	0.5646732559448253
1102	9	0.768387250567649
1102	10	0.842357885922894
1102	11	0.6233667736784341
1102	12	0.4951232020101699
1102	13	0.6799185611853531
1102	14	1.0958520630290778
1102	15	0.982774391744826
1102	16	1.154547793314336
1102	17	1.0798167704690442
1102	18	1.1874896307250609
1102	19	0.9650507649641042
1102	20	0.9564503417317738
1102	21	0.823895354711496
1102	22	1.065605706732768
1102	23	1.2482750193114214
1102	24	1.1119708534828945
1102	25	1.029863771461244
1102	26	0.8634431285116335
1102	27	1.0055279372756303
1102	28	1.0147071676349668
1102	29	0.8440370562841721
1102	30	1.0292216886622754
1102	31	1.0596596837407333
1102	32	1.0482421479484643
1102	33	0.8976769222557763
1102	34	0.8009984226362405
1102	35	0.8118821497274595
1102	36	1.0293869022742257
1102	37	0.9770105675427345
1102	38	1.0454274607621485
1102	39	1.019966507368892
1102	40	0.9412848122820137
1102	41	0.9552181941367408
1102	42	0.988804097095084
1102	43	1.0237608910257379
1102	44	0.9813461049330741
1102	45	0.9715668188599906
1102	46	0.9748548901894836
1102	47	0.9938186737676915
1102	48	0.9567263973169418
1102	49	0.9223739583564381
1102	50	1.0049797135648824
1103	1	0.49331158854025503
1103	2	0.42796684458911116
1103	3	0.5240229766249662
1103	4	0.5264401649399062
1103	5	0.5212911867340679
1103	6	0.45806649187336035
1103	7	0.537498702282484
1103	8	0.6437987021804119
1103	9	0.7428405573393988
1103	10	0.7044067436300168
1103	11	0.6700266804110697
1103	12	0.5927837777132865
1103	13	0.6334536525498038
1103	14	0.7897508490052942
1103	15	0.7060809826954539
1103	16	0.8240968456459967
1103	17	0.9043530747067194
1103	18	0.6666704086045954
1103	19	0.590515678530636
1103	20	0.7818799763322346
1103	21	0.7739526064516511
1103	22	0.7385728291570572
1103	23	0.9673289333714976
1103	24	0.8320108153917687
1103	25	0.5585141470455071
1103	26	0.6708716744100798
1103	27	0.7075794367960526
1103	28	0.8597547314811536
1103	29	0.8660341864501078
1103	30	0.9706084732900919
1103	31	0.868071587859049
1103	32	0.8568818486943783
1103	33	0.9393624350028986
1103	34	0.8036492760779126
1103	35	0.7718347704197015
1103	36	0.8975699070170933
1103	37	0.8838678213150502
1103	38	0.7932409045137163
1103	39	0.9304443005223817
1103	40	0.8588119734250057
1103	41	1.0368057715177414
1103	42	0.9119070831360574
1103	43	1.043834264102827
1103	44	0.9538347124867812
1103	45	0.781270118422043
1103	46	0.8535219190595171
1103	47	0.9287182975633215
1103	48	0.9362313501938999
1103	49	0.894592781186546
1103	50	0.9937175648472341
1104	1	0.5994078262887435
1104	2	0.5788901460340838
1104	3	0.5919440547299573
1104	4	0.5895461578364234
1104	5	0.5528606488360026
1104	6	0.5997201070341376
1104	7	0.5997371862629777
1104	8	0.7216288033315976
1104	9	0.9658656727053199
1104	10	0.8594598070410413
1104	11	0.8515157358487642
1104	12	0.7247346855685564
1104	13	0.7734345186534526
1104	14	1.0021293240466633
1104	15	0.9372544329914021
1104	16	0.9814113144009369
1104	17	1.1584360067401676
1104	18	1.0991749438103682
1104	19	0.9774512054850959
1104	20	1.022200042235923
1104	21	0.8432002514964552
1104	22	0.9203857702367131
1104	23	1.2021427233307804
1104	24	1.0457140971249004
1104	25	1.0857168004434286
1104	26	0.8972339267120049
1104	27	0.9427346415341731
1104	28	1.0259419505840697
1104	29	1.1049284514299273
1104	30	1.376311911685299
1104	31	1.3185386476771157
1104	32	1.215995041592187
1104	33	1.1598499327677487
1104	34	0.9756448736975258
1104	35	1.107273287082137
1104	36	1.1026265569335294
1104	37	1.0378558613096516
1104	38	1.1121551659114317
1104	39	0.9672512340221147
1104	40	1.0775118659471943
1104	41	1.1807325270094537
1104	42	1.2877570812511294
1104	43	1.1938720874328865
1104	44	1.1547158339877583
1104	45	1.1224067047231188
1104	46	1.1989490366489264
1104	47	1.2450634035823285
1104	48	1.2711815447086465
1104	49	1.1545314717468216
1104	50	1.222425934430671
1105	1	0.6102288595812411
1105	2	0.6712833011923323
1105	3	0.6744919153494244
1105	4	0.5772975771108975
1105	5	0.6317658359211435
1105	6	0.4688594924193765
1105	7	0.6017899668143016
1105	8	0.5807175726762992
1105	9	0.8205998231779219
1105	10	0.8760845127173269
1105	11	0.7947538445622087
1105	12	0.8144767277582687
1105	13	0.874088687223022
1105	14	1.078687524528064
1105	15	0.9337539385326181
1105	16	0.9650441023801761
1105	17	0.9742168196829724
1105	18	1.1144576919019897
1105	19	1.0149186684897487
1105	20	0.924761792740938
1105	21	0.9364585013404607
1105	22	0.9038283344158842
1105	23	1.2903735833371428
1105	24	1.0322413353700313
1105	25	1.0398339074862761
1105	26	0.9176006176629201
1105	27	0.9236536447904271
1105	28	0.9297740803614545
1105	29	0.9347047614198516
1105	30	1.0721871761394652
1105	31	1.1370094221110136
1105	32	1.0343803347942568
1105	33	1.0715995579863105
1105	34	0.9392502526502042
1105	35	1.0426247836626388
1105	36	1.0745853095780689
1105	37	0.9952540211568746
1105	38	1.0696745418383387
1105	39	1.0731331899804175
1105	40	1.0186784758556726
1105	41	1.0897669192842372
1105	42	1.164653245775959
1105	43	1.1456628088126593
1105	44	1.0778537967857176
1105	45	1.0696689845311056
1105	46	1.034660843319564
1105	47	1.13597693328812
1105	48	1.2781662411423795
1105	49	1.260511870512964
1105	50	1.1846863507405665
1106	1	0.3679002372092164
1106	2	0.36793166436634905
1106	3	0.3750802622843281
1106	4	0.3868940678633024
1106	5	0.4007624282750655
1106	6	0.4009080970396752
1106	7	0.3579497722873626
1106	8	0.4426815783442919
1106	9	-1.0017105551592635
1106	10	-0.4687464392117491
1106	11	0.10830273386650902
1106	12	0.38589010177025784
1106	13	0.49279577949346276
1106	14	0.7441304543045888
1106	15	0.7002338244306969
1106	16	0.7021880511684557
1106	17	0.7579363552841727
1106	18	-0.8823696511757646
1106	19	0.1493563355364742
1106	20	0.3342832596269787
1106	21	0.4558113767915728
1106	22	0.6253091335859509
1106	23	-0.9039329150211088
1106	24	0.2782394203528469
1106	25	0.4786850327372463
1106	26	0.6645922069987549
1106	27	0.7976013485535347
1106	28	0.814529644542354
1106	29	0.6840298676947398
1106	30	-0.26195592501824905
1106	31	0.5617421830321021
1106	32	0.6833274320876939
1106	33	0.8917678140791949
1106	34	0.7615294869858076
1106	35	0.6654508752723771
1106	36	0.7587777131529592
1106	37	0.6835493347873154
1106	38	0.6775563644438165
1106	39	0.7526771461890291
1106	40	0.7555231140215994
1106	41	0.8632567027674298
1106	42	1.0394177571683159
1106	43	0.9709526656430043
1106	44	0.9144560611294352
1106	45	0.8458663697192605
1106	46	1.0057682355427673
1106	47	0.9566091030083044
1106	48	1.0154768977421256
1106	49	1.0922649460277185
1106	50	1.0766636844715514
1107	1	0.35903797072407073
1107	2	0.3906315395238167
1107	3	0.402991607137821
1107	4	0.39161577069288
1107	5	0.39528362043652976
1107	6	0.4479936452452762
1107	7	0.40051994678446334
1107	8	0.29422233858448976
1107	9	0.5547484925351043
1107	10	0.5712181416619231
1107	11	0.5604408741081741
1107	12	0.6094618975256125
1107	13	0.5671460051703505
1107	14	0.8305901233871325
1107	15	0.721290269073485
1107	16	0.6374720255672628
1107	17	0.9216996798239165
1107	18	0.9253153671551857
1107	19	0.7916546527042954
1107	20	0.7589200299901506
1107	21	0.7766739200769095
1107	22	0.7973525968647337
1107	23	0.9974709985946362
1107	24	0.7939678850156326
1107	25	0.8296014390799229
1107	26	0.7520047183673384
1107	27	0.7294023009721471
1107	28	0.7018880668942558
1107	29	0.6051336225740513
1107	30	0.9141522643130813
1107	31	1.0150968318630476
1107	32	0.9776815555631728
1107	33	0.9333474393256296
1107	34	0.6963395116718658
1107	35	0.6450831829386132
1107	36	0.7474602572291502
1107	37	0.7249508758300038
1107	38	0.8176460703012509
1107	39	0.7994099153772112
1107	40	0.8700902889084787
1107	41	0.8670209028996183
1107	42	0.9813806659428792
1107	43	0.9372827470448613
1107	44	0.9083064365043896
1107	45	0.8762693399547672
1107	46	0.85059616750641
1107	47	1.0161163421806165
1107	48	0.896086197098704
1107	49	1.0268196366611377
1107	50	1.0799426369240557
1108	1	0.1128106843428931
1108	2	0.2922297114411663
1108	3	0.22838874317901414
1108	4	0.13518044083207315
1108	5	0.19966211224733854
1108	6	0.23249037285970076
1108	7	0.11717448831789312
1108	8	0.19809329028958444
1108	9	0.27356710777720394
1108	10	0.13655245574231856
1108	11	0.2440555595467373
1108	12	0.16433049445747372
1108	13	0.29208874630003834
1108	14	0.2556405903156289
1108	15	0.3061845703542261
1108	16	0.41641613068691186
1108	17	0.23689335869670458
1108	18	0.6099032608339883
1108	19	0.40880791182107856
1108	20	0.319300847865982
1108	21	0.39207643365553935
1108	22	0.43081579399279235
1108	23	0.1373694905734908
1108	24	0.3044381884753875
1108	25	0.3835104116551875
1108	26	0.356171399621509
1108	27	0.21045652937604586
1108	28	0.3590464584784101
1108	29	0.39227952258686827
1108	30	0.4364597320180934
1108	31	0.5598645859639433
1108	32	0.43879625325524074
1108	33	0.4495332668537202
1108	34	0.33220915680340823
1108	35	0.4264624442694256
1108	36	0.3814973734406806
1108	37	0.2879287011902747
1108	38	0.3480452614299949
1108	39	0.40744121152477675
1108	40	0.4739762248975765
1108	41	0.35262422688994377
1108	42	0.44521802204298666
1108	43	0.3829452519550145
1108	44	0.3877399815549154
1108	45	0.28444715299151113
1108	46	0.33157792519092766
1108	47	0.3811545065918196
1108	48	0.3871160375300846
1108	49	0.3614454111700667
1108	50	0.3506884481514838
1109	1	-0.24446795973620183
1109	2	-0.25255089448980783
1109	3	-0.30129827720896785
1109	4	-0.28716328117247514
1109	5	-0.4587811773858874
1109	6	-0.3769977331094694
1109	7	-0.4030928304938044
1109	8	-0.36930871781338936
1109	9	-0.19227396069246794
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2210	45	-0.5250572763088073
2210	46	-0.26524012354205695
2210	47	-0.3039443100109551
2210	48	-0.15847103178318278
2210	49	-0.20437520276070842
2210	50	-0.26172624740218
2211	1	-0.054738388490878975
2211	2	-0.11657707726944722
2211	3	-0.11396898902362906
2211	4	-3.127187224976069E-4
2211	5	-0.19520210193915943
2211	6	-0.17813703744546672
2211	7	-0.2011551883823941
2211	8	-0.21822088076216062
2211	9	-0.07053081074138845
2211	10	-0.2069284448301616
2211	11	-0.13561250705918582
2211	12	-0.11266385573761539
2211	13	-0.10866308144203174
2211	14	-0.05478642114219667
2211	15	-0.2979482964858775
2211	16	-0.25768164155514484
2211	17	-0.07535181960009396
2211	18	0.15581605076626204
2211	19	-0.038630239687442725
2211	20	-0.1184113602892225
2211	21	-0.058860450711520684
2211	22	-0.19018131090883372
2211	23	0.005574537988103145
2211	24	0.06407972018234176
2211	25	-0.058334394325811445
2211	26	-0.0587670322503655
2211	27	-0.16705928824502791
2211	28	-0.228134000860166
2211	29	-0.1423603908308806
2211	30	-0.10406308720479274
2211	31	0.061706711889875976
2211	32	-0.05158498878046203
2211	33	-0.05386211406970176
2211	34	-0.16098217508476154
2211	35	-0.12999308371402662
2211	36	-0.0963401012278382
2211	37	0.01509643951467865
2211	38	0.023207792309889896
2211	39	-0.06093580328731463
2211	40	-0.10510707185261481
2211	41	0.004563492201775432
2211	42	0.062074593196683736
2211	43	-0.010766538555472493
2211	44	0.06729955132706067
2211	45	0.054597526946622565
2211	46	-0.09726027430301798
2211	47	-0.11158101417532862
2211	48	-0.04450181957253818
2211	49	0.04264946541747605
2211	50	0.055824015390857795
2212	1	-0.5032967995416691
2212	2	-0.5879982175294636
2212	3	-0.7088874856999219
2212	4	-0.728716353276738
2212	5	-0.6278424492854242
2212	6	-0.5807211471753035
2212	7	-0.7029743362212955
2212	8	-0.534824558082466
2212	9	-0.6887233242844033
2212	10	-0.47151089770468957
2212	11	-0.6280846202303394
2212	12	-0.6909966820371878
2212	13	-0.6779370247654555
2212	14	-0.7306939120679345
2212	15	-0.7546527944228245
2212	16	-0.6872327984429205
2212	17	-0.4319011087114646
2212	18	-0.2766835697618433
2212	19	-0.5595902645094881
2212	20	-0.5934328405804123
2212	21	-0.6535295970568882
2212	22	-0.561477282106388
2212	23	-0.2425805960086933
2212	24	-0.4411212351768441
2212	25	-0.5224106771919921
2212	26	-0.5164974828173854
2212	27	-0.5981042806046801
2212	28	-0.7399494887869338
2212	29	-0.7003488552235595
2212	30	-0.4154905209304971
2212	31	-0.5253297298271136
2212	32	-0.6725774247478142
2212	33	-0.5668010485423878
2212	34	-0.7388890659594693
2212	35	-0.6511949489262179
2212	36	-0.6597626843436899
2212	37	-1.8277724607008423
2212	38	-1.160157458835549
2212	39	-0.779599788882571
2212	40	-0.8340407661457263
2212	41	-1.6780648882906561
2212	42	-1.1372118677095564
2212	43	-0.9340896372828809
2212	44	-0.8299480181668741
2212	45	-0.7003435320274178
2212	46	-0.6912020050784768
2212	47	-0.6702137435323863
2212	48	-0.6983928365974776
2212	49	-0.774301749635125
2212	50	-0.5652853659155284
2213	1	-0.886179348231849
2213	2	-0.8463308282592052
2213	3	-0.8136787805648673
2213	4	-0.830791771665961
2213	5	-0.7155430474342452
2213	6	-0.7995405412505399
2213	7	-0.859741924957973
2213	8	-0.9610091314574767
2213	9	-1.0259757255978812
2213	10	-1.120955146846164
2213	11	-1.0899895326930462
2213	12	-1.053869076364279
2213	13	-1.0716318449376772
2213	14	-2.9029155134249756
2213	15	-1.7924196581907097
2213	16	-1.3699190041716207
2213	17	-1.068663916960162
2213	18	-1.1275390228520337
2213	19	-1.168519147550768
2213	20	-1.5081208516528903
2213	21	-1.4332943173268617
2213	22	-1.3687032345631351
2213	23	-1.870437971485309
2213	24	-1.4832416334223808
2213	25	-1.4148966189434304
2213	26	-1.5149997542622131
2213	27	-1.3909774300568785
2213	28	-1.2861458756437543
2213	29	-1.6404042002240828
2213	30	-1.4477470583886856
2213	31	-1.3417603825080704
2213	32	-1.4151683901055208
2213	33	-1.4292642457587732
2213	34	-1.4020334618442547
2213	35	-1.429432688673657
2213	36	-1.3418086163324148
2213	37	-1.5852807468162489
2213	38	-1.456905276581395
2213	39	-1.4405071570844612
2213	40	-1.6879544237292912
2213	41	-1.832027905525699
2213	42	-1.5569118955667527
2213	43	-1.5747421919796238
2213	44	-1.5408668245488677
2213	45	-1.5655394751656289
2213	46	-1.5640547289357656
2213	47	-1.5234514406714297
2213	48	-1.61358832234761
2213	49	-1.7108601106317423
2213	50	-1.6699298444258162
2214	1	-0.9719269761296445
2214	2	-0.9263586441172293
2214	3	-0.9762237615606821
2214	4	-1.1796744585197168
2214	5	-1.0159719374454994
2214	6	-0.8622808925729544
2214	7	-1.117511608437173
2214	8	-0.9468049920309731
2214	9	-1.1304259726149724
2214	10	-1.4191456301847083
2214	11	-1.2620025001574504
2214	12	-1.2309960965870346
2214	13	-1.2497364079049902
2214	14	-1.729279505019953
2214	15	-1.5739364186135703
2214	16	-1.2997824115008854
2214	17	-1.842005656974493
2214	18	-1.8446683748851882
2214	19	-1.4342586258610623
2214	20	-1.3916493065126616
2214	21	-1.453975105061346
2214	22	-1.353544670073724
2214	23	-1.2687069028969091
2214	24	-1.2301696425428403
2214	25	-1.319312849449588
2214	26	-1.5512031494997913
2214	27	-1.339339924151325
2214	28	-1.2889437371234322
2214	29	-1.1178559945017312
2214	30	-1.0665013795637037
2214	31	-0.9710592691826392
2214	32	-1.122057023836021
2214	33	-1.212286005267984
2214	34	-1.3461190120561355
2214	35	-1.3146629711917726
2214	36	-1.4120586549655485
2214	37	-1.4583914897031178
2214	38	-1.369565258540618
2214	39	-1.3261859018931759
2214	40	-1.3861557851637727
2214	41	-1.316607824422377
2214	42	-1.1570937339290275
2214	43	-1.24832895868607
2214	44	-1.2317598885809034
2214	45	-1.2887489502498148
2214	46	-1.2694987321745899
2214	47	-1.271073740464015
2214	48	-1.4054681623746355
2214	49	-1.3589589594886817
2214	50	-1.2956377845512286
2215	1	-0.4365114482488295
2215	2	-0.6125409515040907
2215	3	-0.5080231242055504
2215	4	-0.39486062346993833
2215	5	-0.43207013467684163
2215	6	-0.6588758705840974
2215	7	-0.4931529890955737
2215	8	-0.5753534976991581
2215	9	-0.6744188444274997
2215	10	-0.9437355604649795
2215	11	-0.7054903719045456
2215	12	-0.8824615020197228
2215	13	-0.8876738051472302
2215	14	-0.7932175295234671
2215	15	-0.8149839930030467
2215	16	-0.7853168493196421
2215	17	-0.8496778580847675
2215	18	-0.7472225085476438
2215	19	-0.9201375366641855
2215	20	-1.0740678793744394
2215	21	-0.9076074057857326
2215	22	-1.2850383396482954
2215	23	-1.1276191800475885
2215	24	-0.9943950416308169
2215	25	-0.8088169712424218
2215	26	-0.886730183819715
2215	27	-0.8612385257245307
2215	28	-0.8480165480309978
2215	29	-0.918298655638317
2215	30	-0.6670087829290168
2215	31	-0.7940050285733307
2215	32	-1.1138911114939845
2215	33	-1.071610147937477
2215	34	-0.9697302718899721
2215	35	-0.9752592138727181
2215	36	-1.007721539817311
2215	37	-0.9205945920428391
2215	38	-0.8703019033363688
2215	39	-1.0944302859821562
2215	40	-1.0213796899332834
2215	41	-1.1411064493342877
2215	42	-1.0169006728432848
2215	43	-1.1153448960357828
2215	44	-1.155750756308354
2215	45	-1.105984869649543
2215	46	-1.0235060678469594
2215	47	-1.0350090078484833
2215	48	-0.9674123492061035
2215	49	-1.0666871174314174
2215	50	-0.9688785890585372
2216	1	-0.28874165478274705
2216	2	-0.5620560593750454
2216	3	-0.4547293240584196
2216	4	-1.7844726285889223
2216	5	-0.6677947575325298
2216	6	-0.5219567225931243
2216	7	-0.488151692800173
2216	8	-0.41138159995601953
2216	9	-0.3416408141619911
2216	10	-0.734906586790153
2216	11	-0.5625649310278362
2216	12	-0.46038242458831746
2216	13	-0.6516719229208192
2216	14	-0.5114380467247415
2216	15	-0.6284753006472599
2216	16	-0.6602742849081125
2216	17	-0.4851601005632631
2216	18	-0.3371405656582951
2216	19	-0.6067597014371202
2216	20	-0.6416080856107058
2216	21	-0.9000874806606589
2216	22	-0.9160296862146993
2216	23	-2.6829273067785735
2216	24	-1.461391666556814
2216	25	-0.9150821741835315
2216	26	-1.041062318349411
2216	27	-1.0217853486556763
2216	28	-1.4985846415010542
2216	29	-1.0810308471257954
2216	30	-0.8858123487433573
2216	31	-0.7346997589864372
2216	32	-0.7071385555998475
2216	33	-0.8005507202737618
2216	34	-1.0745592163878612
2216	35	-1.1051973473853849
2216	36	-1.0314468633901726
2216	37	-0.9790560048914969
2216	38	-1.01767416098334
2216	39	-1.1016538141731402
2216	40	-1.1706469977318505
2216	41	-1.151762542654069
2216	42	-1.365041931120036
2216	43	-1.260868104272589
2216	44	-1.3495956061214223
2216	45	-1.280470113313669
2216	46	-1.4059080744047883
2216	47	-1.41016823100302
2216	48	-1.3829853609907232
2216	49	-1.6867046792830998
2216	50	-1.6501536872571805
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	181115.0
3	8615.0
4	17234.0
5	13059.0
6	10345.0
7	8837.0
8	7978.0
9	7119.0
10	6825.0
11	6778.0
12	6828.0
13	7115.0
14	7498.0
15	7956.0
16	8676.0
17	9485.0
18	10222.0
19	12063.0
20	15359.0
21	20703.0
22	26305.0
23	30105.0
24	31535.0
25	32584.0
26	35089.0
27	40238.0
28	46591.0
29	52158.0
30	60448.0
31	71934.0
32	86891.0
33	108391.0
34	137183.0
35	176818.0
36	233206.0
37	320816.0
38	516216.0
39	1026278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.617504075667895	17.8674343119832	13.198672286927021	24.316389325421888
2	15.710286883090903	20.01883550628061	39.24524023054989	25.025637380078603
3	18.528771313784198	25.387427881882708	29.068818918130567	27.01498188620252
4	12.809858962520938	16.681096418258516	37.37479974863735	33.134244870583196
5	14.074401605316583	37.68313213012936	33.97832940132621	14.26413686322785
6	32.02324669430773	36.840870003275214	17.568203081053532	13.567680221363517
7	28.216563409071888	32.327532405361076	21.64068706560957	17.815217119957474
8	26.54150841976331	34.59214155424161	19.83580506918804	19.03054495680704
9	26.986476273780628	16.066720143194125	18.902053069823875	38.04475051320137
10	14.887005176732405	28.928542997898816	33.19580021684525	22.98865160852353
11	34.50378845397536	23.573444489904652	23.489266414210764	18.433500641909227
12	23.708586704268384	25.048970359272644	29.795746305925206	21.44669663053377
13	27.891073333740664	22.380262211715163	25.756549417907852	23.97211503663632
14	22.629943367566206	21.351035607727205	26.69388376177541	29.32513726293118
15	23.676025365879454	28.946305363000306	23.712132229268303	23.665537041851938
16	23.634216975350895	28.29761769767375	24.938293510786373	23.129871816188984
17	22.654191493612572	27.8540410628871	26.292117665033082	23.19964977846725
18	22.64478517785197	27.448411398624305	27.207671936300247	22.699131487223475
19	23.486381368678323	27.056983220530174	27.349889546907313	22.10674586388419
20	23.75679586335559	26.528935210688292	27.262866418409242	22.451402507546877
21	23.933594569392373	26.441513617943148	27.09924561183941	22.525646200825072
22	23.756610352529613	26.06117438580751	27.06398260134356	23.11823266031932
23	23.53394100629138	26.22032042077259	27.004186809493707	23.241551763442324
24	23.145545367013682	26.35020699208513	27.094432685421964	23.409814955479224
25	23.518462209648206	26.15689022267465	27.100235175220966	23.224412392456177
26	23.13389312254906	26.584473885007476	27.207119169641754	23.07451382280171
27	23.239031108687684	26.58114088691254	26.962831040998786	23.21699696340099
28	23.38186753691488	26.38092694246271	27.018656445599053	23.218549075023354
29	23.32906700891286	26.552110567144954	27.02590176053462	23.092920663407572
30	23.50272234970817	26.44905300187052	26.9874709924994	23.060753655921907
31	23.66579239287796	26.206180551638557	26.84126290258898	23.286764152894506
32	23.266156208262796	26.510912414527795	26.90688883809506	23.316042539114353
33	23.405799868972668	26.33731605496097	26.909400182733183	23.347483893333177
34	23.62814429037844	26.335299914668873	26.94780408208825	23.08875171286444
35	23.60201183491061	26.39679826915681	26.847313435335323	23.15387646059726
36	23.52826932835095	26.489756450716754	26.720257244174235	23.261716976758056
37	23.57824319813445	26.174899740948476	26.974974024325455	23.271883036591614
38	23.580048227770455	26.29750212112879	26.887099541460135	23.235350109640617
39	23.651288968050135	26.48535033942473	26.73254647263807	23.130814219887068
40	23.64003966673364	26.2906962945208	26.95478509348589	23.114478945259673
41	23.49747063466025	26.320202995410046	26.88370382619561	23.298622543734098
42	23.681646491180917	26.34550970970036	26.787117415540397	23.185726383578327
43	23.493796695114327	26.176545819174844	26.83694736375327	23.492710121957558
44	23.51463167094246	26.41253888152763	26.646033614314042	23.426795833215866
45	23.61001226982334	26.50331766860435	26.474449223547484	23.412220838024822
46	23.628354942186323	26.29498206106433	26.489480826615345	23.587182170134
47	23.518484803993537	26.52115695198943	26.483423873146382	23.47693437087065
48	23.508259579396984	26.624221442718625	26.48213694150042	23.38538203638397
49	23.683310864987266	26.371641129115158	26.418075710983647	23.526972294913932
50	23.809390956636857	26.533958938976433	26.307153888827056	23.349496215559654
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2848.0
1	2442.5
2	2037.0
3	1981.0
4	1925.0
5	1821.0
6	1717.0
7	1634.5
8	1552.0
9	1707.0
10	1862.0
11	2471.0
12	3080.0
13	5269.0
14	7458.0
15	10866.5
16	14275.0
17	15418.5
18	16562.0
19	17305.0
20	18048.0
21	20517.0
22	22986.0
23	27506.5
24	32027.0
25	38886.0
26	45745.0
27	53102.5
28	60460.0
29	68252.5
30	76045.0
31	84174.0
32	92303.0
33	102912.5
34	113522.0
35	124762.5
36	136003.0
37	145031.5
38	154060.0
39	164347.0
40	174634.0
41	184207.5
42	193781.0
43	199873.0
44	205965.0
45	214519.0
46	223073.0
47	228110.0
48	233147.0
49	234627.5
50	236108.0
51	236455.0
52	236802.0
53	229677.5
54	222553.0
55	213973.5
56	205394.0
57	192110.0
58	178826.0
59	163601.5
60	148377.0
61	130782.5
62	113188.0
63	97381.5
64	81575.0
65	68517.0
66	55459.0
67	46446.0
68	37433.0
69	30133.5
70	22834.0
71	18148.5
72	13463.0
73	10919.5
74	8376.0
75	6485.0
76	4594.0
77	3655.5
78	2717.0
79	2146.0
80	1575.0
81	1243.5
82	912.0
83	751.5
84	591.0
85	441.5
86	292.0
87	217.0
88	142.0
89	116.0
90	90.0
91	78.0
92	66.0
93	51.0
94	36.0
95	28.0
96	20.0
97	22.5
98	25.0
99	29.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06593091754936599
2	0.07006994665642771
3	0.06707575538748944
4	0.08069639017952232
5	0.08530509634837827
6	0.0657841434675553
7	0.049345446304757005
8	0.06182124325866642
9	0.05832802011157179
10	0.06824994804197504
11	0.05603834443532488
12	0.07326962163990094
13	0.0682205932256129
14	0.06936543106373635
15	0.08251638879397498
16	0.07567671658159641
17	0.08912122247545644
18	0.07341639572171164
19	0.07150833265817255
20	0.08427767777570337
21	0.08154767985402436
22	0.07793703744148117
23	0.07550058768342358
24	0.0621735010550121
25	0.0807550998122466
26	0.08962025435361282
27	0.08184122801764576
28	0.10065766530577738
29	0.09387670272612308
30	0.08028542275045236
31	0.0771444573997034
32	0.08389606516299555
33	0.0800212294031931
34	0.0649034989766911
35	0.08621509565560459
36	0.09208605892803257
37	0.100158633427621
38	0.08066703536316018
39	0.059061890520625285
40	0.06551995012029604
41	0.05832802011157179
42	0.06507962787486395
43	0.04094996882518502
44	0.04050964657975293
45	0.04385609564503686
46	0.042065451846946336
47	0.032172878732905225
48	0.046380609852180885
49	0.053014798350024483
50	0.04312222523598337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3406596.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.704044766249034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85310475448188	47.259983562075426
2	12.296726235531183	14.929220397599877
3	4.229280913462201	7.702033736995562
4	1.9557891125645974	4.748972393698551
5	1.0250894071624328	3.1113536630898
6	0.6219910840095958	2.2654424764755756
7	0.40898063087668457	1.7378744967686925
8	0.2840783276754844	1.3795762816267014
9	0.2060510076467052	1.1257316633084684
>10	1.0430958685233227	11.103829040862957
>50	0.055945838839380646	2.2989685506998803
>100	0.019429090371600243	1.9177975424082425
>500	2.4318270009532334E-4	0.08653586086841046
>1k	1.9454615494979988E-4	0.3326803335217392
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4841	0.14210666600911878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	2.93548163621398E-5
5	0.0	0.0	0.0	0.0	2.93548163621398E-5
6	0.0	0.0	0.0	0.0	5.87096327242796E-5
7	0.0	2.93548163621398E-5	0.0	0.0	5.87096327242796E-5
8	0.0	2.93548163621398E-5	0.0	0.0	5.87096327242796E-5
9	0.0	2.93548163621398E-5	0.0	2.93548163621398E-5	8.806444908641941E-5
10	0.0	2.93548163621398E-5	0.0	2.93548163621398E-5	8.806444908641941E-5
11	0.0	2.93548163621398E-5	0.0	8.806444908641941E-5	8.806444908641941E-5
12	0.0	2.93548163621398E-5	0.0	8.806444908641941E-5	1.4677408181069902E-4
13	0.0	2.93548163621398E-5	2.93548163621398E-5	8.806444908641941E-5	1.4677408181069902E-4
14	0.0	2.93548163621398E-5	2.93548163621398E-5	1.4677408181069902E-4	1.4677408181069902E-4
15	0.0	2.93548163621398E-5	2.93548163621398E-5	1.4677408181069902E-4	1.4677408181069902E-4
16	0.0	2.93548163621398E-5	2.93548163621398E-5	1.4677408181069902E-4	1.4677408181069902E-4
17	0.0	2.93548163621398E-5	2.93548163621398E-5	1.4677408181069902E-4	1.4677408181069902E-4
18	0.0	2.93548163621398E-5	2.93548163621398E-5	1.4677408181069902E-4	1.4677408181069902E-4
19	0.0	2.93548163621398E-5	2.93548163621398E-5	1.4677408181069902E-4	1.4677408181069902E-4
20	0.0	2.93548163621398E-5	2.93548163621398E-5	1.4677408181069902E-4	1.4677408181069902E-4
21	0.0	2.93548163621398E-5	2.93548163621398E-5	1.4677408181069902E-4	1.4677408181069902E-4
22	0.0	2.93548163621398E-5	2.93548163621398E-5	1.7612889817283882E-4	1.7612889817283882E-4
23	0.0	2.93548163621398E-5	2.93548163621398E-5	2.348385308971184E-4	2.0548371453497861E-4
24	0.0	2.93548163621398E-5	2.93548163621398E-5	2.641933472592582E-4	2.0548371453497861E-4
25	0.0	2.93548163621398E-5	2.93548163621398E-5	2.641933472592582E-4	2.0548371453497861E-4
26	0.0	2.93548163621398E-5	2.93548163621398E-5	2.641933472592582E-4	2.0548371453497861E-4
27	0.0	2.93548163621398E-5	2.93548163621398E-5	3.229029799835378E-4	2.0548371453497861E-4
28	0.0	2.93548163621398E-5	2.93548163621398E-5	7.045155926913553E-4	2.348385308971184E-4
29	0.0	2.93548163621398E-5	2.93548163621398E-5	0.0011741926544855921	2.348385308971184E-4
30	0.0	2.93548163621398E-5	2.93548163621398E-5	0.0019374178799012268	2.348385308971184E-4
31	0.0	2.93548163621398E-5	2.93548163621398E-5	0.003992255025251013	2.348385308971184E-4
32	0.0	2.93548163621398E-5	2.93548163621398E-5	0.00587096327242796	2.348385308971184E-4
33	0.0	2.93548163621398E-5	2.93548163621398E-5	0.008336767846847703	2.348385308971184E-4
34	0.0	2.93548163621398E-5	2.93548163621398E-5	0.011037410952164565	2.348385308971184E-4
35	0.0	2.93548163621398E-5	2.93548163621398E-5	0.015000311161053438	2.348385308971184E-4
36	0.0	2.93548163621398E-5	2.93548163621398E-5	0.020078694391703625	2.348385308971184E-4
37	0.0	2.93548163621398E-5	2.93548163621398E-5	0.02518643243871595	2.348385308971184E-4
38	0.0	2.93548163621398E-5	2.93548163621398E-5	0.03102804089478177	2.348385308971184E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2435	0.0	27.646734	1
GTATCAA	5905	0.0	25.893232	1
TAGATCG	90	1.0033707E-5	19.554348	5
TCGATCG	125	2.2982931E-8	19.355106	41
TATCAAC	7950	0.0	19.204409	2
TCAACGC	7980	0.0	18.93896	4
ATCAACG	8075	0.0	18.797325	3
CAACGCA	8180	0.0	18.583204	5
AACGCAG	8340	0.0	18.172602	6
TACCGTC	205	5.456968E-12	17.169418	7
ACCGTCG	170	2.4738256E-9	16.822489	8
ATACCGT	280	0.0	15.712159	6
ACGCAGA	9685	0.0	15.490812	7
GTATTAC	430	0.0	15.348791	1
GAGTACT	5195	0.0	15.203929	12
CGCAGAG	9940	0.0	14.938715	8
AGTACTT	5500	0.0	14.640817	13
AGAGTAC	9755	0.0	14.479536	11
GTACTTT	5460	0.0	14.143438	14
GTACATG	9850	0.0	13.981684	1
>>END_MODULE