FastQCFastQC Report
Mon 6 Feb 2017
SRR2077457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3406596
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55350.1624789085644438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34320.1007457297548638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.028.7742521
GTATCAA64850.025.4450471
TATCAAC93350.017.5585042
AGCGCGA1154.274747E-617.2185523
TCAACGC95250.017.1163654
ATCAACG95500.017.0943473
CAACGCA97850.016.6842925
AACGCAG101400.016.1857836
GTATACG900.00435921614.6676551
ATACCGT2305.966285E-1014.3481616
ACGCAGA117200.013.7601127
GTGGTAT10400.013.3278221
CGCAGAG120850.013.2534918
GTACATG90500.012.9334691
GCACCGT3750.012.9069696
TAGATCG1200.001961485712.8345765
AGAGTAC119600.012.65655811
TACATGG90050.012.558132
CCGCGCT1953.5470966E-612.41218717
ACCGTCG1609.6571406E-512.3756538
Produced by FastQC (version 0.11.5)