FastQCFastQC Report
Mon 6 Feb 2017
SRR2077456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613974
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44540.12324383075251787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57450.026.8462581
GGTATCA22450.026.06881
TATCAAC76400.020.012952
ATCAACG77700.019.5645873
TCAACGC77500.019.5596564
CAACGCA78700.019.2049735
AACGCAG80300.018.6020956
GTATACG1105.6225428E-516.0012191
ATACCGT2353.6379788E-1215.9143426
ACGCAGA93000.015.8964447
GTATTAG5050.015.6843641
CGCAGAG96550.015.1068848
AGAGTAC92350.014.74678911
GAGTACT48350.014.69812112
CGGCACG3800.014.474586522
CAGAGTA98950.014.16417910
AGTACTT49950.013.8749313
GTCTAGG7400.013.6767171
GCAGAGT104500.013.6220899
GTATTAC3750.013.4943611
Produced by FastQC (version 0.11.5)