FastQCFastQC Report
Mon 6 Feb 2017
SRR2077456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613974
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59730.16527512372806222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27050.027.5771771
GTATCAA68900.026.7315181
TATCAAC97050.018.682332
ATCAACG100750.017.9962273
TCAACGC100650.017.9924954
CAACGCA101000.017.9301455
AACGCAG104950.017.190036
CGAATCG3350.017.07220537
ATACCGT2500.016.720876
GTACCGA3700.015.4602626
TACCGAT3600.014.6670227
ACGCAGA122000.014.6249457
GTACATG90700.014.2412511
CGCAGAG125600.014.1532098
TATACTG9500.013.8973825
CGCACGA2855.456968E-1213.89353535
TACATGG94300.013.7903452
TACACCG2754.3655746E-1113.602595
AGAGTAC123400.013.53249411
GCACCGT5700.013.5094726
Produced by FastQC (version 0.11.5)