##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077456_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3613974 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.418665989295995 31.0 31.0 34.0 26.0 34.0 2 30.36797553053785 31.0 30.0 34.0 26.0 34.0 3 30.538637798722405 31.0 31.0 34.0 26.0 34.0 4 33.98833859900486 37.0 35.0 37.0 30.0 37.0 5 33.604834733177384 37.0 35.0 37.0 28.0 37.0 6 33.541808269788326 37.0 35.0 37.0 28.0 37.0 7 33.349115682625275 37.0 35.0 37.0 27.0 37.0 8 33.317153914222956 37.0 35.0 37.0 27.0 37.0 9 34.759480560734524 39.0 35.0 39.0 27.0 39.0 10 34.609982528927986 39.0 35.0 39.0 27.0 39.0 11 34.558482711829136 39.0 35.0 39.0 27.0 39.0 12 34.47966449122213 39.0 35.0 39.0 26.0 39.0 13 34.441114407574595 39.0 35.0 39.0 26.0 39.0 14 35.57909713794289 40.0 36.0 41.0 26.0 41.0 15 35.580920061959496 40.0 36.0 41.0 26.0 41.0 16 35.522834419948786 40.0 36.0 41.0 26.0 41.0 17 35.49579991444321 40.0 36.0 41.0 25.0 41.0 18 35.48600432653915 40.0 36.0 41.0 25.0 41.0 19 35.52413243703469 40.0 36.0 41.0 25.0 41.0 20 35.522078188719675 40.0 36.0 41.0 25.0 41.0 21 35.464127578117605 40.0 36.0 41.0 25.0 41.0 22 35.45709238638684 40.0 35.0 41.0 25.0 41.0 23 35.39429088311095 40.0 35.0 41.0 25.0 41.0 24 35.37767178181138 40.0 35.0 41.0 25.0 41.0 25 35.35188825376165 40.0 35.0 41.0 25.0 41.0 26 35.267281391620415 40.0 35.0 41.0 25.0 41.0 27 35.20626241362002 40.0 35.0 41.0 24.0 41.0 28 35.13807708633211 40.0 35.0 41.0 24.0 41.0 29 35.048967424779484 39.0 35.0 41.0 24.0 41.0 30 34.91705391350353 39.0 35.0 41.0 24.0 41.0 31 34.82670683297666 39.0 34.0 41.0 23.0 41.0 32 34.73897155873285 39.0 34.0 41.0 23.0 41.0 33 34.63861361481848 39.0 34.0 41.0 22.0 41.0 34 34.603313969607974 39.0 34.0 41.0 22.0 41.0 35 34.532187005219185 39.0 34.0 41.0 21.0 41.0 36 34.41664881927762 39.0 34.0 41.0 19.0 41.0 37 34.32117829292629 39.0 34.0 41.0 18.0 41.0 38 34.232152472596646 39.0 34.0 41.0 18.0 41.0 39 34.13506239945279 39.0 34.0 41.0 16.0 41.0 40 34.037991695568365 39.0 33.0 41.0 16.0 41.0 41 33.969706201538806 39.0 33.0 41.0 15.0 41.0 42 33.8446181405843 39.0 33.0 41.0 15.0 41.0 43 33.94006487041689 39.0 33.0 41.0 14.0 41.0 44 33.867766342535944 39.0 33.0 41.0 9.0 41.0 45 33.78372533947394 39.0 33.0 41.0 8.0 41.0 46 33.61630714554117 39.0 33.0 41.0 2.0 41.0 47 33.459978959450176 39.0 33.0 41.0 2.0 41.0 48 33.289021448411084 39.0 33.0 41.0 2.0 41.0 49 33.125677163145056 39.0 33.0 41.0 2.0 41.0 50 32.97612406730098 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6323105253414347 1101 2 0.7941965631853982 1101 3 0.6721892560194469 1101 4 0.6641002365392836 1101 5 0.6986805111583934 1101 6 0.7277605574303436 1101 7 0.7193941912945974 1101 8 0.7940866052921862 1101 9 0.8618396452959942 1101 10 0.8984666173098717 1101 11 0.9821209260825654 1101 12 0.9730966369549918 1101 13 0.9903544221289735 1101 14 1.0738480769247474 1101 15 1.0933295488892725 1101 16 1.0616965675159804 1101 17 1.0943164169537525 1101 18 1.094130136353499 1101 19 1.1035399618878756 1101 20 1.1272656148061841 1101 21 1.1304519366305428 1101 22 1.1561349424716454 1101 23 1.2116308597638579 1101 24 1.15176556175021 1101 25 1.182385101011974 1101 26 1.1002489702704352 1101 27 1.1559020068449897 1101 28 1.149393590199864 1101 29 1.2560242197713478 1101 30 1.297452735607763 1101 31 1.3036683735210204 1101 32 1.3277457875770295 1101 33 1.400571859251663 1101 34 1.303797208132444 1101 35 1.343123680154683 1101 36 1.3364295635250372 1101 37 1.3608483744101036 1101 38 1.3864880097983274 1101 39 1.4203138177552077 1101 40 1.3756872956569168 1101 41 1.382492711196214 1101 42 1.4008011271987044 1101 43 1.3687461651464758 1101 44 1.362014491364718 1101 45 1.3797369750341986 1101 46 1.2845529188245735 1101 47 1.2609897902361595 1101 48 1.4464799533137906 1101 49 1.337988418711511 1101 50 1.4568104298690088 1102 1 0.6088232983983133 1102 2 0.6860170718913388 1102 3 0.6201999174436708 1102 4 0.6644765813603684 1102 5 0.6616855993691573 1102 6 0.6199549643562392 1102 7 0.5925366273753454 1102 8 0.6655703177647467 1102 9 0.68070272414203 1102 10 0.7819932201394408 1102 11 0.7782247857397664 1102 12 0.8036285640203715 1102 13 0.8331970704163822 1102 14 0.9354927109887754 1102 15 0.9240628887948503 1102 16 0.909041969309051 1102 17 1.010843783266786 1102 18 1.012608739027634 1102 19 1.00408202795618 1102 20 1.0291269745551332 1102 21 1.1364877735804058 1102 22 0.9872322502749356 1102 23 0.9958705748643553 1102 24 1.0123449168981153 1102 25 0.9890995819403585 1102 26 0.9061870151339022 1102 27 0.9231071176508294 1102 28 0.8330165459386905 1102 29 0.985071419744294 1102 30 1.010109722848739 1102 31 0.9839913680456718 1102 32 1.051424209202679 1102 33 1.0250090870223119 1102 34 1.0516066006341873 1102 35 1.0776009674209206 1102 36 0.9919289831768552 1102 37 1.0233798294253873 1102 38 0.9610156538725576 1102 39 1.0258383150686257 1102 40 0.985794348848188 1102 41 1.0270445982093008 1102 42 0.8292567534098652 1102 43 0.8252475399204329 1102 44 0.9658968156070102 1102 45 0.862176855208304 1102 46 1.0592209546284224 1102 47 1.1635449772824131 1102 48 1.1746792620770137 1102 49 1.2010849666292742 1102 50 1.2376660638517194 1103 1 0.46667304233605833 1103 2 0.5862660927463033 1103 3 0.4875925520311064 1103 4 0.4989624934134369 1103 5 0.45165828866313973 1103 6 0.5542869588181816 1103 7 0.6179850334113013 1103 8 0.6204880717101489 1103 9 0.6502871679298323 1103 10 0.6905694700059684 1103 11 0.658056615074635 1103 12 0.6902237926814649 1103 13 0.767406718307285 1103 14 0.7880768699279344 1103 15 0.8049152779584006 1103 16 0.8374585833277948 1103 17 0.8745713771542114 1103 18 0.9092267023704892 1103 19 0.741881868398373 1103 20 0.7967340082946208 1103 21 0.8939979339505442 1103 22 0.876542444770152 1103 23 0.9076917580255781 1103 24 0.840866389271163 1103 25 0.8960898314613814 1103 26 0.8588545002822201 1103 27 0.8646321610964591 1103 28 0.9095289485607196 1103 29 1.0059380903602957 1103 30 1.015065273335594 1103 31 0.9053402948615599 1103 32 0.9543560218297387 1103 33 0.9084192254346348 1103 34 0.9166669172454007 1103 35 0.9316115334254533 1103 36 0.9130511662171159 1103 37 1.0698742223767468 1103 38 0.9737508003283892 1103 39 0.9550547755599723 1103 40 0.8830160281355717 1103 41 0.9347849964511141 1103 42 1.0063909277620624 1103 43 0.9410284384600445 1103 44 0.9297169586982292 1103 45 0.9508834401781598 1103 46 0.9638837459819314 1103 47 0.9531916337162869 1103 48 0.9713495127047977 1103 49 1.02086385597498 1103 50 1.055611918488104 1104 1 0.5579881994767781 1104 2 0.7644404891590568 1104 3 0.6920172653972791 1104 4 0.6478023558332495 1104 5 0.6177873913987355 1104 6 0.7028011756808965 1104 7 0.7443238968022925 1104 8 0.7206590234220158 1104 9 0.7434796867026634 1104 10 0.8786679304641183 1104 11 0.9082109549572976 1104 12 0.8192800423543076 1104 13 0.8419205106241563 1104 14 0.959958230465169 1104 15 0.8532852559489044 1104 16 0.9887498893202675 1104 17 1.0715283174167354 1104 18 1.0003768048504966 1104 19 0.9410917193403776 1104 20 0.9645197255669729 1104 21 1.0469382172579955 1104 22 0.9199546801364917 1104 23 1.0184602790992017 1104 24 0.9818262081037119 1104 25 0.9632343324751318 1104 26 0.9297486330790008 1104 27 0.9538764105735922 1104 28 1.003687558452441 1104 29 0.9970511889314722 1104 30 1.0419412845046239 1104 31 1.0603141715526547 1104 32 1.0569414864390794 1104 33 1.087317728117199 1104 34 1.0582562314678725 1104 35 1.0955750827444177 1104 36 1.1057628922365623 1104 37 1.0453943459504842 1104 38 1.0297178262223028 1104 39 1.0672532705111593 1104 40 1.0437179257829214 1104 41 1.0161073545503996 1104 42 1.2146024825717348 1104 43 1.2101806290750545 1104 44 1.1705293373025825 1104 45 1.092742533157292 1104 46 1.034605709993599 1104 47 1.1102828342453535 1104 48 1.1154703392725906 1104 49 1.11827581772652 1104 50 1.0896040069540547 1105 1 0.3832833967025131 1105 2 0.5313088281375151 1105 3 0.3896072309972034 1105 4 0.3714722908628687 1105 5 0.38528009126912366 1105 6 0.42252946994464935 1105 7 0.40443028958124216 1105 8 0.4143074839793286 1105 9 0.37182690627781056 1105 10 0.4747696804220496 1105 11 0.4108828198697765 1105 12 0.4733126838643784 1105 13 0.49092847374489423 1105 14 0.48443033741970254 1105 15 0.5312578505061865 1105 16 0.516755856528782 1105 17 0.552631178778455 1105 18 0.45111111345266863 1105 19 0.5792589233976102 1105 20 0.5326314388331426 1105 21 0.6409670999125865 1105 22 0.6670825470823729 1105 23 0.7126759610591762 1105 24 0.6472550221569833 1105 25 0.635577601553571 1105 26 0.6994158569687912 1105 27 0.736196638254782 1105 28 0.6628006266777717 1105 29 0.74179355006207 1105 30 0.802103842958509 1105 31 0.8414203208101583 1105 32 0.7880024347639107 1105 33 0.8019060470886927 1105 34 0.7708123322501237 1105 35 0.6905706881951588 1105 36 0.6992244856268712 1105 37 0.750961988604189 1105 38 0.7295388785070713 1105 39 0.697310976305701 1105 40 0.6989633563457716 1105 41 0.7508133883345636 1105 42 0.8061591322527519 1105 43 0.7083125172633871 1105 44 0.7763626819744616 1105 45 0.8072157927415518 1105 46 0.8621229683078937 1105 47 0.8576225651882581 1105 48 0.8349472740728743 1105 49 0.8393836709894558 1105 50 0.8721836357014183 1106 1 0.5572987609382452 1106 2 0.6980589962042529 1106 3 0.58520934545286 1106 4 0.5682745280286596 1106 5 0.638559809100137 1106 6 0.6611892604939271 1106 7 0.6499340550868666 1106 8 0.6397383881270216 1106 9 0.7535786016570896 1106 10 0.7752737969453491 1106 11 0.7439567162949743 1106 12 0.6590414374240723 1106 13 0.740768973215971 1106 14 0.765970309570001 1106 15 0.7242492402181568 1106 16 0.7396594334005542 1106 17 0.8964505484622975 1106 18 0.7615822940480328 1106 19 0.691687177698249 1106 20 0.6769869744803785 1106 21 0.7822547180864916 1106 22 0.7209978776801478 1106 23 0.8627887117443009 1106 24 0.7208361058127153 1106 25 0.7549556448349861 1106 26 0.7672970633970166 1106 27 0.7413182799107929 1106 28 0.7985283480255987 1106 29 0.8240772464544577 1106 30 0.8896453870247214 1106 31 0.7819563180750109 1106 32 0.9473554345176538 1106 33 0.9888502506381158 1106 34 0.8733475660392216 1106 35 0.8297412053621898 1106 36 0.9048574020067761 1106 37 0.8686755943480264 1106 38 0.9221983193843215 1106 39 0.9743484041768156 1106 40 1.0334413563005427 1106 41 0.9805660930168401 1106 42 1.022612156224909 1106 43 1.0204614277908348 1106 44 0.9866379261106175 1106 45 0.9330058573888635 1106 46 0.9038436592590315 1106 47 0.9228815267975605 1106 48 0.9106473295169266 1106 49 1.0364637027901153 1106 50 1.0846612490936707 1107 1 0.3603375866512941 1107 2 0.503430691566269 1107 3 0.37485863198586955 1107 4 0.4291855359110315 1107 5 0.3768241409234818 1107 6 0.41246081280328895 1107 7 0.4909502043187999 1107 8 0.40224565959960756 1107 9 0.4033987683602973 1107 10 0.5389095132448176 1107 11 0.45589107731902345 1107 12 0.544601471585402 1107 13 0.530461441927919 1107 14 0.5660740693478346 1107 15 0.5574556392301346 1107 16 0.6260344465341916 1107 17 0.6397731291428244 1107 18 0.5507570281067586 1107 19 0.5600324540161381 1107 20 0.600608664795331 1107 21 0.6738551618664204 1107 22 0.6507803330220625 1107 23 0.6221528752829215 1107 24 0.5460003641779849 1107 25 0.617432111514951 1107 26 0.5893647177133232 1107 27 0.6159387427424861 1107 28 0.603570420496709 1107 29 0.6631480691623679 1107 30 0.7290286436008415 1107 31 0.7374420705036826 1107 32 0.6639039032724341 1107 33 0.7157421896056704 1107 34 0.7399279521969007 1107 35 0.7229384545251705 1107 36 0.8058382147360206 1107 37 0.7246866665296068 1107 38 0.7004277094397509 1107 39 0.7025959862166715 1107 40 0.65018207121296 1107 41 0.6671548047244258 1107 42 0.7678509420645838 1107 43 0.7376889113159777 1107 44 0.7428700433189874 1107 45 0.7119451895556637 1107 46 0.6976716197811683 1107 47 0.7706249048152571 1107 48 0.8427233745272673 1107 49 0.8281303291448197 1107 50 0.8176744473606234 1108 1 0.3001194039635813 1108 2 0.41781028629934625 1108 3 0.28979354718121186 1108 4 0.2834258070234199 1108 5 0.28940485858424125 1108 6 0.2834286819308147 1108 7 0.2966437526422965 1108 8 0.2910230222649517 1108 9 0.3054414344225478 1108 10 0.32875149875332 1108 11 0.34632931368940945 1108 12 0.25482982327699943 1108 13 0.29497154205731135 1108 14 0.28913247326320857 1108 15 0.3439538095089887 1108 16 0.3410369515814935 1108 17 0.339042232783612 1108 18 0.2843488411110542 1108 19 0.3015026693917022 1108 20 0.24972257506414763 1108 21 0.3293799963037145 1108 22 0.23549926468012927 1108 23 0.41122369543121096 1108 24 0.36457592267506556 1108 25 0.34177305640174893 1108 26 0.31945785917491065 1108 27 0.3494298832442837 1108 28 0.4451407105721543 1108 29 0.44177375149998 1108 30 0.41771505123582386 1108 31 0.5378670064020667 1108 32 0.4909288292244014 1108 33 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-1.9842567766183166 2216 16 -2.0379360703823934 2216 17 -1.9207220391164341 2216 18 -2.088952403168882 2216 19 -1.9862871807137452 2216 20 -2.0197686585644234 2216 21 -2.10822106523608 2216 22 -2.0980718351527514 2216 23 -2.0700340564674917 2216 24 -2.215704317767859 2216 25 -2.242954628671889 2216 26 -2.4366477601610015 2216 27 -2.4185266556542686 2216 28 -2.539137465885915 2216 29 -2.3360410340111954 2216 30 -2.2965639860179863 2216 31 -2.174886118524057 2216 32 -2.260308752624127 2216 33 -2.2777592803046574 2216 34 -2.256709760740385 2216 35 -2.3656696201879086 2216 36 -2.5003671857102 2216 37 -2.365132060462237 2216 38 -2.4849765312742207 2216 39 -2.538877343540147 2216 40 -2.577388043384822 2216 41 -2.585467443086692 2216 42 -2.656188154633611 2216 43 -2.408389786348888 2216 44 -2.4044812594344407 2216 45 -2.3208147560622514 2216 46 -2.281306254450179 2216 47 -2.2864095520893635 2216 48 -2.290310375110682 2216 49 -2.2386944069955774 2216 50 -2.2921473349550396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97997.0 3 15355.0 4 26610.0 5 15557.0 6 11903.0 7 9101.0 8 7858.0 9 7044.0 10 6593.0 11 6604.0 12 6871.0 13 7162.0 14 7666.0 15 8300.0 16 9146.0 17 9794.0 18 10463.0 19 11736.0 20 13479.0 21 16883.0 22 21073.0 23 25683.0 24 29979.0 25 33275.0 26 36443.0 27 41501.0 28 48406.0 29 55860.0 30 64108.0 31 76613.0 32 92755.0 33 114646.0 34 144662.0 35 183896.0 36 239498.0 37 321578.0 38 504874.0 39 1273002.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61926464084698 17.96138725162713 13.996969074709872 26.422379032816018 2 14.884161342619942 19.836998253157418 38.036646338949446 27.242194065273196 3 17.558327886869993 24.51735849202787 29.37315957039994 28.551154050702195 4 12.352050825836836 16.488623125645077 36.15178573899184 35.00754030952625 5 14.018694175806745 37.38029640402716 33.97834062131408 14.62266879885201 6 32.89194538495327 36.447110252236584 16.962482946982796 13.69846141582736 7 28.4964157671371 31.75512129152508 21.373398208146515 18.375064733191305 8 27.051989973261474 34.07292108053413 19.5194815366915 19.355607409512892 9 26.82794984638379 14.954191923385645 18.72761493537048 39.490243294860086 10 14.61404411003539 28.486711687149896 33.38727007916689 23.51197412364782 11 35.27957535128575 22.463714358633954 23.373422805713137 18.883287484367152 12 23.861449860278945 24.34052907152971 29.98559191486358 21.81242915332777 13 28.387623236312837 21.36586953759723 25.84559964325584 24.400907582834094 14 22.66913553043385 20.313011251618704 26.955524465613163 30.062328752334285 15 23.927205690013825 28.217940041160976 23.739476200955256 24.11537806786994 16 23.916553700264448 27.361574518655168 25.02079624496634 23.701075536114047 17 22.71914280944945 27.06511174553803 26.51718724523227 23.698558199780255 18 22.780581199761258 26.47354060569418 27.623875632154128 23.122002562390442 19 23.660419030766075 26.20637933798111 27.640328188443974 22.492873442808843 20 23.918825619642988 25.65892520102028 27.505991990747226 22.9162571885895 21 24.11537589517141 25.482345443910067 27.46498972814251 22.937288932776013 22 23.718351359873147 25.299146624131215 27.487495013646207 23.495007002349436 23 23.47001993134758 25.36208614771343 27.437769903665156 23.730124017273834 24 23.337915593766283 25.520099477312275 27.341340385917494 23.80064454300395 25 23.435161794479573 25.414679840754967 27.51134518425509 23.638813180510365 26 23.342322655583462 25.69195727866585 27.517430076231154 23.448289989519534 27 23.502231051014792 25.758555960915984 27.284927595688547 23.454285392380683 28 23.452078032230702 25.633198023726795 27.424472436480883 23.490251507561617 29 23.602128913478495 25.630061006818732 27.378273556025707 23.389536523677062 30 23.564844379081656 25.77263770128191 27.265306393604487 23.397211526031946 31 23.675956066343364 25.352781283907973 27.20581722358777 23.7654454261609 32 23.42893030720145 25.785247676274707 27.15331231017363 23.63250970635021 33 23.617363348501073 25.57413183119111 27.10813912842281 23.700365691885004 34 23.730118073135433 25.573822485953855 27.18715379342086 23.50890564748985 35 23.65388351637281 25.639632618426756 27.21508582587513 23.491398039325304 36 23.674879192290447 25.649843074521613 27.067956758312622 23.607320974875318 37 23.70943204797984 25.407966178763147 27.188446403352458 23.694155369904557 38 23.682349714541278 25.55523236746836 27.150308567746944 23.612109350243422 39 23.787270089201087 25.80292185931672 26.91933989037285 23.490468161109344 40 23.817164027319297 25.565008139836383 27.147343629495346 23.470484203348967 41 23.638357037762255 25.5501155174098 27.040869835442 23.770657609385953 42 23.861290218141157 25.662211751136837 26.884733107695837 23.591764923026176 43 23.6705257574177 25.385242665038877 27.048270133216523 23.895961444326904 44 23.805411310634288 25.67990042719126 26.717080289708615 23.79760797246584 45 23.805013434277193 25.781886000957417 26.55814095061001 23.854959614155383 46 23.87393706759463 25.560572239557867 26.54553574615634 24.01995494669116 47 23.79002802459789 25.79057147157916 26.484158133110196 23.935242370712746 48 23.769482059322087 25.99963585711653 26.35561596318283 23.875266120378555 49 23.93363086401682 25.755699620002275 26.342561322468455 23.968108193512453 50 24.01114232334099 25.931247869377927 26.188084675901948 23.869525131379138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 908.0 1 656.0 2 404.0 3 687.5 4 971.0 5 1076.5 6 1182.0 7 1140.0 8 1098.0 9 1435.0 10 1772.0 11 2664.5 12 3557.0 13 6669.0 14 9781.0 15 13466.5 16 17152.0 17 19029.5 18 20907.0 19 21834.5 20 22762.0 21 24414.5 22 26067.0 23 29438.0 24 32809.0 25 38585.0 26 44361.0 27 50268.5 28 56176.0 29 63707.5 30 71239.0 31 78625.0 32 86011.0 33 95816.5 34 105622.0 35 116465.5 36 127309.0 37 138015.0 38 148721.0 39 159699.5 40 170678.0 41 182762.5 42 194847.0 43 203579.0 44 212311.0 45 223153.0 46 233995.0 47 243253.5 48 252512.0 49 255426.0 50 258340.0 51 260353.5 52 262367.0 53 256806.0 54 251245.0 55 242882.5 56 234520.0 57 220584.5 58 206649.0 59 189029.0 60 171409.0 61 150467.0 62 129525.0 63 110875.0 64 92225.0 65 77370.0 66 62515.0 67 51557.5 68 40600.0 69 32999.5 70 25399.0 71 20092.0 72 14785.0 73 12214.5 74 9644.0 75 7381.0 76 5118.0 77 4005.0 78 2892.0 79 2277.5 80 1663.0 81 1294.0 82 925.0 83 705.5 84 486.0 85 385.0 86 284.0 87 206.0 88 128.0 89 87.5 90 47.0 91 38.5 92 30.0 93 21.5 94 13.0 95 10.5 96 8.0 97 6.0 98 4.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015384726066097875 2 6.917592655619547E-4 3 2.2136296497982555E-4 4 0.0017709037198386044 5 0.03887687072458186 6 0.027033952098161194 7 0.029524285454184233 8 0.023104759469769292 9 0.02971797804854158 10 0.03043740768472601 11 0.038406474423999734 12 0.039264255913296554 13 0.035999152179844125 14 0.04194828186367694 15 0.03677392255727351 16 0.04269538187048385 17 0.04468764855530228 18 0.04764837821190745 19 0.049280930078633656 20 0.04571145226833397 21 0.04640321153389593 22 0.04468764855530228 23 0.04355316335978067 24 0.04128419296873746 25 0.03951328924889885 26 0.04261237075861641 27 0.04291674483546368 28 0.042003622604921895 29 0.03948561887827638 30 0.03644187810980378 31 0.034698644760587656 32 0.03135052991526779 33 0.022772715022299553 34 0.019507611288847124 35 0.020144029813164124 36 0.021970274274247685 37 0.017238640897803914 38 0.018456137205192954 39 0.01253467789198262 40 0.00733264821495672 41 0.006447196355037418 42 0.0058937889425878555 43 0.00229664076166569 44 0.0037631704046570337 45 0.002324311132288168 46 9.131222305417804E-4 47 1.6602222373486916E-4 48 2.2136296497982555E-4 49 1.3835185311239097E-4 50 1.6602222373486916E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3613974.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.33523773218927 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26929613978652 43.15582988109216 2 13.42709057149234 15.396908599363007 3 4.80389242110674 8.262969420120516 4 2.2152720845902083 5.080526064458484 5 1.198718674297481 3.4364410082430417 6 0.7438506251546609 2.5589311458288058 7 0.478059707036818 1.9186766867195701 8 0.3293890076104379 1.5108477646171485 9 0.25171603859470665 1.298897902244919 >10 1.1922920213503794 11.943969600331773 >50 0.06535789209702343 2.564466034679738 >100 0.024240893719876633 2.2736741654931882 >500 6.78524961376252E-4 0.25159377898329444 >1k 9.693213425341818E-5 0.18028941862483858 >5k 4.846606712670909E-5 0.16597852919946993 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5973 0.16527512372806222 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 2.767037062247819E-5 13 0.0 0.0 0.0 0.0 5.534074124495638E-5 14 0.0 0.0 0.0 0.0 5.534074124495638E-5 15 0.0 0.0 0.0 0.0 5.534074124495638E-5 16 0.0 0.0 0.0 5.534074124495638E-5 5.534074124495638E-5 17 0.0 0.0 0.0 5.534074124495638E-5 5.534074124495638E-5 18 0.0 0.0 0.0 5.534074124495638E-5 5.534074124495638E-5 19 0.0 0.0 0.0 5.534074124495638E-5 5.534074124495638E-5 20 0.0 0.0 0.0 5.534074124495638E-5 8.301111186743457E-5 21 0.0 0.0 0.0 1.1068148248991276E-4 8.301111186743457E-5 22 0.0 0.0 0.0 1.3835185311239097E-4 8.301111186743457E-5 23 0.0 0.0 0.0 1.6602222373486914E-4 8.301111186743457E-5 24 0.0 0.0 0.0 2.2136296497982552E-4 8.301111186743457E-5 25 0.0 0.0 0.0 2.490333356023037E-4 1.1068148248991276E-4 26 0.0 0.0 0.0 3.3204444746973827E-4 1.1068148248991276E-4 27 0.0 0.0 0.0 4.980666712046074E-4 1.1068148248991276E-4 28 0.0 0.0 0.0 7.747703774293893E-4 1.1068148248991276E-4 29 0.0 0.0 0.0 0.0015218703842363006 1.1068148248991276E-4 30 0.0 0.0 0.0 0.002324311132288168 1.1068148248991276E-4 31 0.0 0.0 0.0 0.004952996341423596 1.1068148248991276E-4 32 0.0 0.0 0.0 0.007720033403671415 1.1068148248991276E-4 33 0.0 0.0 0.0 0.010597751948409147 1.1068148248991276E-4 34 0.0 0.0 0.0 0.013945866793729009 1.1068148248991276E-4 35 0.0 0.0 0.0 0.017985740904610824 1.1068148248991276E-4 36 0.0 0.0 0.0 0.02291106687541194 1.1068148248991276E-4 37 0.0 0.0 0.0 0.029939341013521404 1.1068148248991276E-4 38 0.0 0.0 0.0 0.037382670710968034 1.1068148248991276E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2705 0.0 27.577177 1 GTATCAA 6890 0.0 26.731518 1 TATCAAC 9705 0.0 18.68233 2 ATCAACG 10075 0.0 17.996227 3 TCAACGC 10065 0.0 17.992495 4 CAACGCA 10100 0.0 17.930145 5 AACGCAG 10495 0.0 17.19003 6 CGAATCG 335 0.0 17.072205 37 ATACCGT 250 0.0 16.72087 6 GTACCGA 370 0.0 15.460262 6 TACCGAT 360 0.0 14.667022 7 ACGCAGA 12200 0.0 14.624945 7 GTACATG 9070 0.0 14.241251 1 CGCAGAG 12560 0.0 14.153209 8 TATACTG 950 0.0 13.897382 5 CGCACGA 285 5.456968E-12 13.893535 35 TACATGG 9430 0.0 13.790345 2 TACACCG 275 4.3655746E-11 13.60259 5 AGAGTAC 12340 0.0 13.532494 11 GCACCGT 570 0.0 13.509472 6 >>END_MODULE