FastQCFastQC Report
Mon 6 Feb 2017
SRR2077455_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077455_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2966212
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.18488226734973764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32770.11047760578138044No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30640.10329672997075057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33350.023.878411
GTATCAA71000.023.7645591
TATCAAC91350.018.1812522
ATCAACG93700.017.4200633
TCAACGC93950.017.3745884
CAACGCA94750.017.0421435
GGTATCG800.00199130816.49746944
AACGCAG98050.016.4007036
GTACATG117050.015.6554771
TACATGG117000.015.323472
CGCGATC1057.1586686E-414.66788123
GCGATAC900.004359569414.66738714
ACCGTCG1809.071482E-814.6659028
ATACCGT2753.6379788E-1214.3990066
ACATGGG121200.014.3568533
GAGTACT54850.014.23905112
ACGCAGA113800.014.1117427
AGTACTT57650.014.04381713
AGAGTAC107900.014.027781511
CGCAGAG115250.013.9341978
Produced by FastQC (version 0.11.5)