FastQCFastQC Report
Mon 6 Feb 2017
SRR2077455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2966212
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77920.26269194514754846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47400.15979977156049535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44750.1508658180871765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.028.4154361
GTATCAA92650.023.9388731
ACGTGCG550.004483808820.0010137
CGCAATT600.007418184518.33178934
TATCAAC129800.017.0014342
ATCAACG134700.016.4159153
TCAACGC134950.016.4023634
CAACGCA137700.016.107295
AACGCAG141050.015.7543416
GTCTACG1301.3683115E-515.2330811
CCGTTAA2051.8153514E-915.02312536
CGTCCGA1809.0562025E-814.66790311
TCGCGTC2850.014.66765717
TCGCTAA1951.517401E-814.6674099
GTAATAC3200.014.4389613
GTACATG112500.014.297281
TACATGG114000.013.9152082
GCGTATC950.006236382313.8968461
CGCGATC1751.0532876E-613.82950423
ACGCAGA163400.013.4780397
Produced by FastQC (version 0.11.5)