FastQCFastQC Report
Mon 6 Feb 2017
SRR2077454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134173
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50530.16122275317922782No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36680.11703246757597618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89250.018.8091071
GTACATG118000.015.99768451
TACATGG120100.015.5330292
GGTATCA50950.015.3729661
CAACGCA113100.014.6067275
TCAACGC114150.014.5696524
TATCAAC114000.014.5695252
ATCAACG114350.014.5432413
ACATGGG125250.014.4894463
AACGCAG116050.014.1975126
TATCGCT1100.001018978413.9993747
AGAGTAC106900.013.93358311
GTATTAG5400.013.8527811
GTATTAC4950.013.7787041
TACACCG2401.1314114E-913.74850755
TCGATCG1453.8522085E-513.65042141
ACCGTAT2451.537046E-913.4687868
GTATTAT6750.013.363861
TAGTCGG2155.4977136E-813.29812140
GAGTACT53250.013.26307412
Produced by FastQC (version 0.11.5)