FastQCFastQC Report
Mon 6 Feb 2017
SRR2077454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3134173
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65540.209114174616398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40330.12867828291546127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37860.12079741609668643No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34400.10975782128172248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG500.002579118822.0032711
GTATCAA101550.021.1690771
GGTATCA52200.019.1790961
TATCAAC137900.015.5887262
ATCAACG141150.015.3077353
TCAACGC142100.015.1434594
CAACGCA142650.015.039285
AACGCAG145550.014.7979766
GTAATAC3600.014.6686133
TCGATCG1201.18497344E-414.66229541
TAGTCGG1554.5853103E-614.18954440
GTACATG109650.013.5651731
GAGTACT95400.013.23844212
AGAGTAC148100.013.23698611
TACATGG111700.013.0993212
AGTACTT97250.013.07752313
ATTATAC6250.012.6736823
ACGCAGA169100.012.6460387
GTACTTT99200.012.62082814
ACATGGG115150.012.5348823
Produced by FastQC (version 0.11.5)