FastQCFastQC Report
Mon 6 Feb 2017
SRR2077453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091741
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.023.9526121
GTATCAA44650.023.5526351
ATCAACG58500.017.5995663
TCAACGC59200.017.3920274
CAACGCA59400.017.2964295
TATCAAC60400.017.2283332
AACGCAG61150.016.6916146
CGCGATC953.345335E-416.21248823
GTACATG76150.015.2544691
TACATGG76550.014.8868762
ACGCAGA69950.014.4032877
CGCACGA1707.6021024E-714.23517435
TTAGTCG1251.676613E-414.07577939
CGCAGAG71950.013.9422228
GTGCCGA3000.013.93389211
GTATAGA5700.013.895071
ACATGGG79700.013.8014163
TAATACT7500.013.7867734
GTATTAG4700.013.5747931
AGAGTAC68250.013.41006211
Produced by FastQC (version 0.11.5)