##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077453_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3091741
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.59181477361784	31.0	30.0	34.0	16.0	34.0
2	28.573712675156166	31.0	29.0	34.0	16.0	34.0
3	28.66084675268724	31.0	30.0	34.0	16.0	34.0
4	32.00590023549838	35.0	33.0	37.0	19.0	37.0
5	32.05940536416213	35.0	33.0	37.0	19.0	37.0
6	32.1341380147949	36.0	33.0	37.0	19.0	37.0
7	31.917014394155267	35.0	33.0	37.0	17.0	37.0
8	31.84652271972329	35.0	32.0	37.0	17.0	37.0
9	33.04306085147495	38.0	33.0	39.0	16.0	39.0
10	32.92203001480396	38.0	33.0	39.0	15.0	39.0
11	32.99015409117387	38.0	33.0	39.0	15.0	39.0
12	32.90929673604613	38.0	33.0	39.0	15.0	39.0
13	32.90991159996908	38.0	33.0	39.0	13.0	39.0
14	33.70535112740686	39.0	33.0	41.0	10.0	41.0
15	33.80080446583333	39.0	33.0	41.0	11.0	41.0
16	33.69378676933158	39.0	33.0	41.0	10.0	41.0
17	33.499127514238744	38.0	32.0	41.0	10.0	41.0
18	33.30450998321011	38.0	32.0	41.0	10.0	41.0
19	33.655064573649604	39.0	33.0	41.0	10.0	41.0
20	33.70209568007152	39.0	33.0	41.0	10.0	41.0
21	33.695632331427504	39.0	33.0	41.0	10.0	41.0
22	33.59081468984627	39.0	33.0	41.0	10.0	41.0
23	33.31719183463298	39.0	32.0	41.0	10.0	41.0
24	33.469786440714145	39.0	32.0	41.0	10.0	41.0
25	33.362669447408436	39.0	32.0	41.0	9.0	41.0
26	33.403440973872	39.0	32.0	41.0	8.0	41.0
27	33.38476605899395	39.0	33.0	41.0	8.0	41.0
28	33.25708751153476	39.0	32.0	41.0	8.0	41.0
29	33.21995632881279	38.0	32.0	41.0	8.0	41.0
30	32.98975463986149	38.0	32.0	40.0	2.0	41.0
31	32.91633484176068	38.0	32.0	40.0	2.0	41.0
32	32.933764827002	38.0	32.0	40.0	2.0	41.0
33	32.92115898453331	38.0	32.0	40.0	2.0	41.0
34	32.89816029221076	38.0	32.0	40.0	2.0	41.0
35	32.82553357477227	38.0	32.0	40.0	2.0	41.0
36	32.69171835545086	38.0	32.0	40.0	2.0	41.0
37	32.58750037600174	38.0	31.0	40.0	2.0	41.0
38	32.477102706856755	38.0	31.0	40.0	2.0	41.0
39	32.409645892071815	38.0	31.0	40.0	2.0	41.0
40	32.36407448101248	38.0	31.0	40.0	2.0	41.0
41	32.242862516620896	38.0	31.0	40.0	2.0	41.0
42	32.056997659247656	38.0	31.0	40.0	2.0	41.0
43	31.939428626136536	38.0	31.0	40.0	2.0	41.0
44	31.86866752422017	38.0	31.0	40.0	2.0	41.0
45	31.77845168790012	38.0	31.0	40.0	2.0	41.0
46	31.61527792916677	38.0	30.0	40.0	2.0	41.0
47	31.424901697781284	38.0	30.0	40.0	2.0	41.0
48	31.230077810528112	38.0	30.0	40.0	2.0	41.0
49	31.086423474670095	38.0	30.0	40.0	2.0	41.0
50	30.87736391890524	37.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8383604926711641
1101	2	0.8332381060516063
1101	3	0.8728495779026844
1101	4	0.9121353808858004
1101	5	0.715137434782882
1101	6	0.8522325561578796
1101	7	0.8854719349535465
1101	8	0.9138679450140934
1101	9	1.1451149150917388
1101	10	1.0811988398510195
1101	11	1.150368064640297
1101	12	1.1376765150697352
1101	13	1.0425222314898193
1101	14	1.2860295131543893
1101	15	1.3864095135924046
1101	16	1.1878966779943525
1101	17	1.3723803667628047
1101	18	1.5524563999992864
1101	19	1.2210879644439956
1101	20	1.2329996195052786
1101	21	1.2571868679390406
1101	22	1.190003359307788
1101	23	1.2432156874361908
1101	24	1.1753644738519426
1101	25	1.2938963237691965
1101	26	1.2309881584011464
1101	27	1.332269767232141
1101	28	1.3430627396574124
1101	29	1.3272040209570477
1101	30	1.460265249960436
1101	31	1.4388326301042653
1101	32	1.4545482324630186
1101	33	1.4708208925442747
1101	34	1.399870388377181
1101	35	1.3429395771288384
1101	36	1.3560691262801043
1101	37	1.352324529646019
1101	38	1.3331935438984246
1101	39	1.2606404704792311
1101	40	1.2413346977386652
1101	41	1.3700967562172792
1101	42	1.3765203432558621
1101	43	1.3761271324804376
1101	44	1.1307596033566512
1101	45	1.2513580037473453
1101	46	1.2326548172127083
1101	47	1.2496194799657125
1101	48	1.2239157960380744
1101	49	1.2537721474536703
1101	50	1.3127875234380397
1102	1	0.4760982335263293
1102	2	0.4517499060045935
1102	3	0.4363930040012889
1102	4	0.3879910975519465
1102	5	0.4108696358318724
1102	6	0.5360655416463516
1102	7	0.5053462692097277
1102	8	0.5928615063971243
1102	9	0.7079775802344841
1102	10	0.7186887858394542
1102	11	0.4867424612790856
1102	12	0.4513679913698425
1102	13	0.5817326355574366
1102	14	0.8764094125747377
1102	15	0.7800557639546994
1102	16	1.041781927951881
1102	17	1.041737986050002
1102	18	1.0893397499390574
1102	19	0.8965154009472869
1102	20	0.7708086327205592
1102	21	0.7769348653804826
1102	22	0.9101595529935125
1102	23	1.171833314116732
1102	24	0.8617765989465056
1102	25	0.8234777278539696
1102	26	0.7488712343202835
1102	27	0.8643155250129482
1102	28	0.8177502047660639
1102	29	0.7145990431920595
1102	30	0.9732627174390487
1102	31	0.9586083959977003
1102	32	0.9193386066834037
1102	33	0.7561363373379848
1102	34	0.6405727296626367
1102	35	0.7609828712308797
1102	36	0.7946031274961314
1102	37	0.7895551009375623
1102	38	0.8946112371461226
1102	39	0.9840147674215629
1102	40	0.9268502778650145
1102	41	0.9306179527058802
1102	42	0.776994981306629
1102	43	0.806918363666103
1102	44	0.7818172342718839
1102	45	0.7637683323773352
1102	46	0.7499397143951931
1102	47	0.8614954876568071
1102	48	0.8386774086986399
1102	49	0.8610759059714965
1102	50	0.7941644375569687
1103	1	0.407131291961484
1103	2	0.3844938767777748
1103	3	0.49563796338614097
1103	4	0.29177456170376814
1103	5	0.31776839525738154
1103	6	0.4630539337474602
1103	7	0.546413600240836
1103	8	0.5897809946017212
1103	9	0.591415970542073
1103	10	0.5838115008638525
1103	11	0.5611464952929737
1103	12	0.5868398564273392
1103	13	0.6635422894944369
1103	14	0.8748985052368354
1103	15	0.6412772944929586
1103	16	0.8776180412460164
1103	17	0.9963096764292132
1103	18	0.63222506120227
1103	19	0.6755511811896184
1103	20	0.8540596270388932
1103	21	0.8998031544790166
1103	22	0.8327706617494925
1103	23	0.9879689348985536
1103	24	0.8010979080661897
1103	25	0.6895526405963537
1103	26	0.6664289558720213
1103	27	0.886588907934474
1103	28	0.8604067059612035
1103	29	0.8752595814446451
1103	30	1.0569582880891844
1103	31	0.9806176418810182
1103	32	0.878147359510919
1103	33	0.8576638755264412
1103	34	0.8160705953701495
1103	35	0.7925608345930115
1103	36	0.8640244282460401
1103	37	0.8445282143958366
1103	38	0.7594122049964014
1103	39	0.8144148752503426
1103	40	0.7869978685113068
1103	41	0.9349680614585978
1103	42	0.8866868540382313
1103	43	0.8615577731296007
1103	44	0.8769804829183201
1103	45	0.7515886800599425
1103	46	0.7520952030650214
1103	47	0.8190411654291871
1103	48	0.6861866399076781
1103	49	0.6511152221717929
1103	50	0.8988420544973934
1104	1	0.6920705325996046
1104	2	0.7059760759507618
1104	3	0.6936882438464913
1104	4	0.6743826841632767
1104	5	0.6911552572681074
1104	6	0.7452485681755192
1104	7	0.6979531957321932
1104	8	0.7019230381331703
1104	9	0.9528811769017622
1104	10	1.0285633864328005
1104	11	0.9719877391548835
1104	12	0.9698714367119905
1104	13	0.9133184154750467
1104	14	1.2663227536919877
1104	15	1.0529495267664117
1104	16	1.0921913049467804
1104	17	1.3002777858688006
1104	18	1.353304390502963
1104	19	1.162810423827338
1104	20	1.0992209413076495
1104	21	0.9826470188650163
1104	22	1.0278938540078926
1104	23	1.27576403750286
1104	24	1.0463751763915852
1104	25	1.1187943555662514
1104	26	1.0797199875868628
1104	27	1.1240763470280584
1104	28	1.09267856165647
1104	29	1.1584749216589216
1104	30	1.3769427160419667
1104	31	1.41742122454189
1104	32	1.221436344646058
1104	33	1.1778196035158928
1104	34	1.128739064680758
1104	35	1.0829080070523958
1104	36	1.1404896731317962
1104	37	1.1367569622442488
1104	38	1.1498256140964074
1104	39	0.987576754442756
1104	40	1.157096577925742
1104	41	1.2643210885642233
1104	42	1.2876749630154976
1104	43	1.256702793692181
1104	44	1.257785068760306
1104	45	1.1638869608766136
1104	46	1.2534151905938522
1104	47	1.1258771644842618
1104	48	1.1669267086870398
1104	49	1.0633739902811605
1104	50	1.093851688832423
1105	1	0.6531416455686738
1105	2	0.722531252807098
1105	3	0.5478037092547723
1105	4	0.6545194473114648
1105	5	0.5822715661954234
1105	6	0.4825431556694184
1105	7	0.5803590920343957
1105	8	0.644273644514751
1105	9	0.834903435688048
1105	10	0.9699726582988362
1105	11	0.8136908665723723
1105	12	0.9003155189870853
1105	13	0.8937886066726932
1105	14	1.1054912328584123
1105	15	0.9859892524962746
1105	16	0.875884646353299
1105	17	0.9403549448014701
1105	18	1.1795212448166623
1105	19	0.929756118756103
1105	20	0.8305893240718945
1105	21	0.9174756892885867
1105	22	0.967075685173171
1105	23	1.207468935268949
1105	24	1.0184445800072623
1105	25	1.076716777105588
1105	26	1.0074774914846074
1105	27	0.8609240702705065
1105	28	0.9575520561163202
1105	29	0.848298876111869
1105	30	1.0342450692666816
1105	31	1.1011324553953443
1105	32	0.9761824409530249
1105	33	0.9825190979995426
1105	34	0.8509845733509991
1105	35	0.9671489663730597
1105	36	0.893140175077697
1105	37	0.8124824704384324
1105	38	0.8023251529131628
1105	39	0.849533078901537
1105	40	0.8737770181028353
1105	41	1.0698959014130835
1105	42	1.010444137824777
1105	43	0.804981799143075
1105	44	0.8982382408084391
1105	45	0.8405526878204235
1105	46	0.8793957628776319
1105	47	1.0409908089625581
1105	48	1.0655346419315102
1105	49	1.0301377409803756
1105	50	0.9386042045126537
1106	1	0.5035486541987808
1106	2	0.3798194902278027
1106	3	0.40415184123341064
1106	4	0.4590566838511876
1106	5	0.5435530397907158
1106	6	0.4820010118062683
1106	7	0.4572329364751049
1106	8	0.4147350133345036
1106	9	-0.9202618962291496
1106	10	-0.48192863520308293
1106	11	0.09350416043074006
1106	12	0.4671283542441742
1106	13	0.5894905872071305
1106	14	0.6866496665575639
1106	15	0.6878935797941352
1106	16	0.7315132253268999
1106	17	0.862010696443889
1106	18	-1.207144742038217
1106	19	0.01853581010947636
1106	20	0.20106774554167828
1106	21	0.3854236351461111
1106	22	0.5650131386247779
1106	23	-0.9051745949755485
1106	24	0.19780350179436823
1106	25	0.5375055984640937
1106	26	0.5763489157711987
1106	27	0.5666153446749007
1106	28	0.6964495855378061
1106	29	0.792854625146127
1106	30	-0.3351749649436684
1106	31	0.5016052023082977
1106	32	0.5883534687994683
1106	33	0.7336764232311666
1106	34	0.7468861142657701
1106	35	0.7404428591516492
1106	36	0.8129629706038344
1106	37	0.6814026075261594
1106	38	0.7690523807880041
1106	39	0.7572552397032268
1106	40	0.7400204415577178
1106	41	0.9065590446502725
1106	42	1.0148139359967487
1106	43	0.8735186455456976
1106	44	0.8787026325568021
1106	45	0.8353737264617429
1106	46	0.8837350419680199
1106	47	0.8218805570144134
1106	48	0.9396573315374148
1106	49	0.9551458824208261
1106	50	0.9662962863595759
1107	1	0.17509952688751795
1107	2	0.22652119836382667
1107	3	0.19234631809813507
1107	4	0.1719182262965333
1107	5	0.25571368170960085
1107	6	0.16424137337916989
1107	7	0.2711440245489207
1107	8	0.08053600293633068
1107	9	0.368090332162339
1107	10	0.421037591475546
1107	11	0.25561933096111744
1107	12	0.30681896739697123
1107	13	0.2211421950055339
1107	14	0.49112571940450067
1107	15	0.2950708029635436
1107	16	0.1595823084019301
1107	17	0.5395461231773311
1107	18	0.5187690941490075
1107	19	0.4218608903880181
1107	20	0.39311226668620947
1107	21	0.2743106269854465
1107	22	0.35813659484512783
1107	23	0.5063184396365017
1107	24	0.39602243014574157
1107	25	0.4416057420499939
1107	26	0.3451317738180393
1107	27	0.3955605800852666
1107	28	0.3001066109540318
1107	29	0.34548521188665404
1107	30	0.5374994116287866
1107	31	0.6445310645150926
1107	32	0.5126262889494768
1107	33	0.3222377657660189
1107	34	0.28368236927654067
1107	35	0.3767251056223131
1107	36	0.32407831747089233
1107	37	0.31534903763311917
1107	38	0.41415933125447424
1107	39	0.4169564739621947
1107	40	0.5087611939969321
1107	41	0.5069014205963498
1107	42	0.5397017292209512
1107	43	0.4627896476404878
1107	44	0.4673404054396002
1107	45	0.35107902918061384
1107	46	0.5279914769453562
1107	47	0.5241268178185905
1107	48	0.5539640967214225
1107	49	0.5668879403561888
1107	50	0.6787220557739744
1108	1	-0.07211945052242896
1108	2	0.0028088844464058127
1108	3	0.014885819325474614
1108	4	-0.008789518725798473
1108	5	0.06061023099470475
1108	6	0.06465188245370967
1108	7	0.10420384352094558
1108	8	0.04588677432776578
1108	9	0.08002824431434163
1108	10	-0.04733608984476945
1108	11	0.12544857671073828
1108	12	0.06563525284144589
1108	13	0.2377393877927787
1108	14	0.012677225880075582
1108	15	0.018207320324272303
1108	16	0.22109172058622306
1108	17	0.06427775095639277
1108	18	0.47261598698538876
1108	19	0.18042064065154761
1108	20	0.09192597622187293
1108	21	0.22837006748890332
1108	22	0.17871134977749392
1108	23	-0.09407751836844369
1108	24	0.06231968967084356
1108	25	0.1864080454473651
1108	26	0.10999986142216045
1108	27	0.0923258790745578
1108	28	0.15877156175575635
1108	29	0.23174951244735809
1108	30	0.31190654351795644
1108	31	0.3870553425436327
1108	32	0.2771704525013874
1108	33	0.30422232755624634
1108	34	0.1307753488517278
1108	35	0.23292842717226847
1108	36	0.2841834018820535
1108	37	0.25940914850628616
1108	38	0.1779112796813962
1108	39	0.3327138747294427
1108	40	0.3814015937836075
1108	41	0.30264108911700305
1108	42	0.2847488671205767
1108	43	0.4361938290231713
1108	44	0.297859417634875
1108	45	0.1887204996233791
1108	46	0.20798987931885193
1108	47	0.23529873140388702
1108	48	0.29264245843637937
1108	49	0.07021700338167491
1108	50	0.17512176573790228
1109	1	-0.13002332776344616
1109	2	-0.09913262645805077
1109	3	-0.13362407662720344
1109	4	-0.07902894805698679
1109	5	-0.17762881387888285
1109	6	-0.15971795879734074
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2210	48	0.07367131833611396
2210	49	0.10486352797074616
2210	50	0.04585195451475954
2211	1	0.207913053697812
2211	2	0.1858849617692293
2211	3	0.14290987084212503
2211	4	0.3335589039240787
2211	5	0.12369893849952973
2211	6	0.16849488575411442
2211	7	0.1777242850287699
2211	8	0.23366811137547572
2211	9	0.3803494415824602
2211	10	0.24210911262135681
2211	11	0.309928610436792
2211	12	0.29174460396947666
2211	13	0.19684637531152305
2211	14	0.40592676869602684
2211	15	0.22075589658354744
2211	16	0.2562119059701615
2211	17	0.5067093010860901
2211	18	0.7399442728598444
2211	19	0.4603808601067314
2211	20	0.4868905733483757
2211	21	0.27257493550042255
2211	22	0.31444983477597077
2211	23	0.5167944947904601
2211	24	0.4499327296715734
2211	25	0.4105465686901155
2211	26	0.3604420502287695
2211	27	0.25986338064136305
2211	28	0.18892943219530878
2211	29	0.3202605411287749
2211	30	0.4603973085411184
2211	31	0.484479228010386
2211	32	0.4367931995087275
2211	33	0.4396498428118747
2211	34	0.30668549393305966
2211	35	0.23881395356642088
2211	36	0.4162693274487381
2211	37	0.29741077038502794
2211	38	0.41161963081891173
2211	39	0.3548206236059315
2211	40	0.2659472733548185
2211	41	0.4067914308334082
2211	42	0.45524333678066853
2211	43	0.3148150629206974
2211	44	0.37777107984184255
2211	45	0.3950919231829637
2211	46	0.14221441715988448
2211	47	0.24241724278886423
2211	48	0.2755515118126972
2211	49	0.2894209379285897
2211	50	0.34478137199441505
2212	1	-0.21717098108286237
2212	2	-0.2701680098638448
2212	3	-0.4458662469744539
2212	4	-0.368350563341739
2212	5	-0.18911607869393166
2212	6	-0.1576964617995209
2212	7	-0.2927333549022286
2212	8	-0.13435828730474242
2212	9	-0.28388159250820166
2212	10	-0.13180343047014276
2212	11	-0.20624824660633578
2212	12	-0.22388929029741433
2212	13	-0.2379642722976527
2212	14	-0.3739979127430928
2212	15	-0.3800327819761051
2212	16	-0.457864767431019
2212	17	-0.18021677470662922
2212	18	-0.014308798954111523
2212	19	-0.19942356286364316
2212	20	-0.24431619388222714
2212	21	-0.20363628948606305
2212	22	-0.10655445181313183
2212	23	0.16453894968551452
2212	24	-0.007261339468414008
2212	25	-0.31510707014831496
2212	26	-0.31545606205353494
2212	27	-0.29513848545617094
2212	28	-0.29653571267441947
2212	29	-0.17102832921704447
2212	30	-0.06728766762235239
2212	31	-0.2963851021261661
2212	32	-0.30083305540519945
2212	33	-0.3245075654055469
2212	34	-0.3564979632114884
2212	35	-0.3664535316294675
2212	36	-0.3820072111240904
2212	37	-1.4797132980975611
2212	38	-0.7760073746751601
2212	39	-0.506867600240188
2212	40	-0.5007947187746176
2212	41	-1.272168185711589
2212	42	-0.7367534255444177
2212	43	-0.5734535333063526
2212	44	-0.45810037908062995
2212	45	-0.5930093540628789
2212	46	-0.6214468316985027
2212	47	-0.45479820839521423
2212	48	-0.5021911813758493
2212	49	-0.5552545241570179
2212	50	-0.44267210282068703
2213	1	-0.7557490547527941
2213	2	-0.7390011889004029
2213	3	-0.7527381959966064
2213	4	-0.7937993490826436
2213	5	-0.6822288171298894
2213	6	-0.7273554375231228
2213	7	-0.6867953830839824
2213	8	-0.6991705316454713
2213	9	-0.7693355122992998
2213	10	-0.859414921676283
2213	11	-0.7371333273411196
2213	12	-0.6764684652755832
2213	13	-0.8385442161534229
2213	14	-2.629986583746234
2213	15	-1.5542146591303379
2213	16	-1.3532707940187976
2213	17	-0.8429534712001043
2213	18	-0.9925127177349111
2213	19	-1.1265165800107084
2213	20	-1.230974296184769
2213	21	-1.193094399449265
2213	22	-1.214328303403235
2213	23	-1.7080306564913705
2213	24	-1.2552338552566766
2213	25	-1.1261112970424065
2213	26	-1.2245979570451624
2213	27	-1.2224017590931382
2213	28	-0.9917125518881633
2213	29	-1.435908948493843
2213	30	-1.1228288399112039
2213	31	-0.9733717309299443
2213	32	-1.0421172496817377
2213	33	-1.0314217798545755
2213	34	-0.9614876204372074
2213	35	-1.0744826273917987
2213	36	-1.0701510428931087
2213	37	-1.1278943824785408
2213	38	-0.8962027461936231
2213	39	-0.9679436546400346
2213	40	-1.0803967877160225
2213	41	-1.375790959177511
2213	42	-1.188262655326195
2213	43	-1.2377712080208738
2213	44	-1.3216779739590905
2213	45	-1.2353447899203722
2213	46	-1.277218298292123
2213	47	-1.198468189821618
2213	48	-1.1825410347426661
2213	49	-1.3565579965987489
2213	50	-1.2565691087136024
2214	1	-0.6002781605454359
2214	2	-0.6336448364746978
2214	3	-0.758186219018981
2214	4	-0.7799896744266022
2214	5	-0.643188570976033
2214	6	-0.5951448604504712
2214	7	-0.8888232184593399
2214	8	-0.7188636095494019
2214	9	-0.9763304771808023
2214	10	-1.0881260799402561
2214	11	-0.9129474090495293
2214	12	-1.0435986370474062
2214	13	-0.9576645138319542
2214	14	-1.3199095478552607
2214	15	-1.1361471188764654
2214	16	-0.7981272929471572
2214	17	-1.373558954864393
2214	18	-1.5544529566662888
2214	19	-1.1333655306484118
2214	20	-1.059429559969729
2214	21	-1.0710283588431935
2214	22	-0.9317779910104704
2214	23	-0.7063171005457463
2214	24	-0.9554957274072038
2214	25	-0.9586132672319394
2214	26	-1.161714495144345
2214	27	-0.945764284469611
2214	28	-0.9558326915884621
2214	29	-0.7745281772583823
2214	30	-0.7492028020903945
2214	31	-0.6040878685121669
2214	32	-0.7464650742653021
2214	33	-0.7507396302838956
2214	34	-0.8866552988777272
2214	35	-0.9720697271012178
2214	36	-0.9174637207996135
2214	37	-1.044937095686155
2214	38	-0.9321439824409268
2214	39	-0.8207443796023561
2214	40	-0.9459459453333032
2214	41	-0.8986168685929243
2214	42	-0.6882084759245579
2214	43	-0.7691901391034222
2214	44	-0.7392076102214524
2214	45	-0.8360721640554836
2214	46	-0.827709805092816
2214	47	-0.813261937030255
2214	48	-0.8660331394401801
2214	49	-0.825790169230789
2214	50	-0.8391747156484612
2215	1	-0.5380817968901006
2215	2	-0.6751714510748066
2215	3	-0.5749582146214394
2215	4	-0.4605497506393803
2215	5	-0.583704927712418
2215	6	-0.6233759742178684
2215	7	-0.5370902328341742
2215	8	-0.5919706700972966
2215	9	-0.6732274410028012
2215	10	-1.0047755266729474
2215	11	-0.7779441456173117
2215	12	-0.8041695775101303
2215	13	-0.7360150259344493
2215	14	-0.7586317708790418
2215	15	-0.7925191587901921
2215	16	-0.7028751772184378
2215	17	-0.759026056068457
2215	18	-0.8542485835611444
2215	19	-1.016897641117808
2215	20	-0.9709746106998622
2215	21	-0.9507910359548006
2215	22	-1.317844142130653
2215	23	-1.2500713021077416
2215	24	-1.0561189137609617
2215	25	-0.9901768967424331
2215	26	-0.968258420567075
2215	27	-1.0485227018790155
2215	28	-0.9143858389073856
2215	29	-0.9678393190895846
2215	30	-0.7708468937292992
2215	31	-0.9379790869490492
2215	32	-1.0422418742497186
2215	33	-1.0855116534382496
2215	34	-1.0494700395324763
2215	35	-0.9676185066297371
2215	36	-1.0360996509642
2215	37	-0.9134689929951918
2215	38	-0.911211675244477
2215	39	-0.8803762569484945
2215	40	-0.8993955525517023
2215	41	-1.10839470984331
2215	42	-1.005424521658746
2215	43	-1.0285641209366183
2215	44	-0.9802524497883205
2215	45	-1.0123634899610536
2215	46	-0.9816210858735985
2215	47	-0.9802154531532921
2215	48	-1.0174256227780063
2215	49	-1.128175225865828
2215	50	-1.1316812361955293
2216	1	-1.1919842048933802
2216	2	-1.4368567810940789
2216	3	-1.2158593220396234
2216	4	-2.6650041147233914
2216	5	-1.663634852023364
2216	6	-1.515128541540026
2216	7	-1.41267302512734
2216	8	-1.4627688981386875
2216	9	-1.3410937392138713
2216	10	-1.7302854944063526
2216	11	-1.6026400821001232
2216	12	-1.6919894694127464
2216	13	-1.7094116641066037
2216	14	-1.5855761866246638
2216	15	-1.8370062556955347
2216	16	-1.900386199742396
2216	17	-1.7182430717646184
2216	18	-1.4900816610916081
2216	19	-1.791611536640584
2216	20	-1.840644570913966
2216	21	-1.9865217674714444
2216	22	-2.2792471254572817
2216	23	-3.7014443387818545
2216	24	-2.6217087964248407
2216	25	-2.179500760334289
2216	26	-2.2830676351397443
2216	27	-2.254107683571238
2216	28	-2.59975576715701
2216	29	-2.3346318047139576
2216	30	-2.1295673223890184
2216	31	-1.9980554510994502
2216	32	-2.1208367873096883
2216	33	-2.231555275069809
2216	34	-2.5405241251487958
2216	35	-2.657129159156444
2216	36	-2.567521108044705
2216	37	-2.446073919105771
2216	38	-2.4895820163841798
2216	39	-2.5948992458250792
2216	40	-2.6580276965850693
2216	41	-2.7063532350909583
2216	42	-2.952647397633953
2216	43	-2.8526545599018043
2216	44	-2.8069672956669365
2216	45	-2.7041662605392602
2216	46	-2.6976499486430363
2216	47	-2.8130114947794738
2216	48	-2.9051282867209167
2216	49	-3.1618236411996783
2216	50	-3.246372961721722
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	192894.0
3	9485.0
4	18153.0
5	13805.0
6	11340.0
7	9630.0
8	8377.0
9	7739.0
10	7232.0
11	7274.0
12	7243.0
13	7599.0
14	7869.0
15	8647.0
16	9053.0
17	9942.0
18	10882.0
19	12383.0
20	15135.0
21	20029.0
22	24856.0
23	28723.0
24	31034.0
25	33157.0
26	36259.0
27	40752.0
28	46157.0
29	52102.0
30	59987.0
31	71362.0
32	85789.0
33	105748.0
34	131451.0
35	167439.0
36	218298.0
37	295755.0
38	461498.0
39	806663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88381305744585	17.56206465093428	12.710914303431785	24.843207988188087
2	15.898682095622673	19.9583959357872	38.825421526785654	25.31750044180448
3	18.9279594831565	24.857315228318292	28.81397349497262	27.400751793552597
4	12.795958928941372	16.317523824321526	37.255735964367105	33.63078128237
5	14.20645446579071	37.364366581129815	34.153287169116616	14.27589178396286
6	32.506019081759504	36.42846346816271	17.54785246865692	13.517664981420868
7	28.53326214110623	31.71251284291597	21.66881052350557	18.085414492472232
8	26.68707735077519	34.44189670489355	19.90138791573149	18.96963802859977
9	26.975641124805456	15.742797307762885	18.812066716268795	38.469494851162864
10	14.773717373601633	28.686535416658575	33.57513862294946	22.96460858679033
11	35.15030695693541	22.98089650195307	23.4070439901489	18.46175255096262
12	23.719288228767336	24.62518595049347	29.981725387534425	21.67380043320477
13	28.309337103337207	21.8089933753057	25.628517386424104	24.253152134932996
14	22.598226561953812	20.94299182545786	26.694819251215048	29.76396236137328
15	23.968339158926362	28.707786570297827	23.439180226087824	23.884694044687986
16	24.118133544378573	27.75144567608116	24.797394950774994	23.333025828765273
17	23.024158774308333	27.582853576358406	26.176375756539844	23.216611892793416
18	23.088428548308787	27.131639423854992	26.915261369153587	22.864670658682634
19	23.834646673429187	26.80149198369486	26.935814535188946	22.428046807687004
20	23.967955281380224	26.40698204023523	26.816410682047646	22.808651996336895
21	24.191146170696328	26.142741024667416	26.807537314471595	22.85857549016466
22	23.935530064627898	25.90667118105975	26.68666637750382	23.471132376808534
23	23.856696363257363	26.04694400459885	26.518157818757143	23.57820181338664
24	23.397705915612573	26.24762401639214	26.666198455355783	23.688471612639507
25	23.746587399159548	26.053851925655312	26.68033972898592	23.51922094619922
26	23.564656382213137	26.372815271225402	26.778124563976874	23.284403782584583
27	23.597757369959503	26.398843724811684	26.562250140002526	23.441148765226284
28	23.658506183092047	26.33998831210248	26.568242266480823	23.43326323832465
29	23.66710846564101	26.445747755015887	26.550024830958474	23.337118948384628
30	23.792668352489827	26.424468777959483	26.538604961913602	23.24425790763709
31	23.92067829483711	26.076829306977356	26.46570816153589	23.53678423664965
32	23.578603762249063	26.41009887298266	26.474419399614657	23.536877965153618
33	23.639407007074144	26.26979507833222	26.534808456862734	23.5559894577309
34	23.866370154503723	26.200772308241028	26.522745419537628	23.410112117717617
35	23.898149700788768	26.28073607970161	26.489468528598092	23.33164569091153
36	23.903579198176732	26.31413161711385	26.313710763493216	23.4685784212162
37	23.88918243049863	26.05794770383551	26.517653290234517	23.535216575431345
38	23.856315569697696	26.19372861549816	26.517656274783363	23.432299540020782
39	23.908111596344504	26.332477001037567	26.371377659371337	23.388033743246595
40	23.977927195345938	26.115170198477234	26.598732917445446	23.308169688731383
41	23.71405667823852	26.289066330438914	26.50346977591037	23.493407215412194
42	23.996947343619325	26.211838558752632	26.439719745948565	23.351494351679477
43	23.81043906618674	26.045569797195768	26.475504680844452	23.668486455773042
44	23.77155456239518	26.310099657948577	26.331099693218285	23.587246086437958
45	23.86131610301175	26.31484426116518	26.174666689964877	23.649172945858194
46	23.935659543074035	26.150331587624397	26.094255339458684	23.819753529842885
47	23.824406986292594	26.348334928421625	26.167342557020362	23.65991552826542
48	23.78915267707653	26.41060307273724	26.201786637689732	23.5984576124965
49	23.902715279901493	26.203187270335476	26.156651634815	23.737445814948035
50	24.033827719379424	26.317667977730785	26.073360266505734	23.575144036384053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1740.0
1	1455.0
2	1170.0
3	1137.0
4	1104.0
5	1125.5
6	1147.0
7	1099.5
8	1052.0
9	1200.0
10	1348.0
11	1722.5
12	2097.0
13	3416.5
14	4736.0
15	6778.0
16	8820.0
17	9887.0
18	10954.0
19	11797.5
20	12641.0
21	14799.0
22	16957.0
23	21236.0
24	25515.0
25	31297.0
26	37079.0
27	43509.5
28	49940.0
29	57561.5
30	65183.0
31	73418.5
32	81654.0
33	90995.5
34	100337.0
35	110218.5
36	120100.0
37	129544.5
38	138989.0
39	147998.0
40	157007.0
41	166232.5
42	175458.0
43	182838.5
44	190219.0
45	198122.5
46	206026.0
47	212368.0
48	218710.0
49	219355.0
50	220000.0
51	219706.0
52	219412.0
53	213372.0
54	207332.0
55	198571.5
56	189811.0
57	178143.0
58	166475.0
59	151798.0
60	137121.0
61	120800.5
62	104480.0
63	89766.0
64	75052.0
65	63361.0
66	51670.0
67	42827.0
68	33984.0
69	28009.5
70	22035.0
71	17675.5
72	13316.0
73	10996.0
74	8676.0
75	6812.0
76	4948.0
77	3957.5
78	2967.0
79	2412.5
80	1858.0
81	1439.5
82	1021.0
83	849.5
84	678.0
85	506.5
86	335.0
87	282.5
88	230.0
89	188.5
90	147.0
91	112.5
92	78.0
93	59.0
94	40.0
95	36.0
96	32.0
97	28.0
98	24.0
99	30.0
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06400924268882807
2	0.0691843204201128
3	0.06672615849775256
4	0.07959916435432333
5	0.08487127479306966
6	0.06417096386793072
7	0.049033861503922874
8	0.06003090168290293
9	0.05886650919336387
10	0.06679084696939362
11	0.05598787220533674
12	0.07144841692754988
13	0.06756710862908633
14	0.0695077627783181
15	0.08082824531550346
16	0.07620301959316773
17	0.08658551929155774
18	0.07248343247380683
19	0.06996058207980552
20	0.08273655522891471
21	0.0816368512110167
22	0.07642942924391144
23	0.07351844802006377
24	0.06365345609480225
25	0.07940509893940016
26	0.08868789461989215
27	0.08121637614534982
28	0.09949086938394905
29	0.09234279326761201
30	0.07921103352447698
31	0.07591192147078298
32	0.08166919544683723
33	0.0796638528259644
34	0.06604692954552144
35	0.08470955361396702
36	0.08994931981689282
37	0.09784131335710204
38	0.07992260671252863
39	0.058834164957543345
40	0.06358876762316118
41	0.05731398587397844
42	0.0638151772739049
43	0.041174212199534176
44	0.04107717949207259
45	0.0444733242532282
46	0.04224157198161165
47	0.03244126852799119
48	0.04744899394871692
49	0.052915169802386426
50	0.04450566848904872
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3091741.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.95491976041046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.73761613196916	51.79350457738469
2	11.551305271234181	15.006282140420454
3	4.036969581923398	7.866631058071564
4	1.6831315687299064	4.373107039722595
5	0.9081311468304992	2.949379288715231
6	0.5196892604605978	2.0253824528139113
7	0.33149569128993545	1.507259322006169
8	0.2198862686291865	1.1426155948179897
9	0.164815327140627	0.9635009714734706
>10	0.7925839505968961	8.923803853233157
>50	0.040063782426475784	1.7478020289204637
>100	0.014011492545969647	1.4556823619886727
>500	1.5026311165896924E-4	0.06451577044825414
>1k	1.5026311144022956E-4	0.1805335399833481
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.468847164105919E-5	0.0	0.0	0.0	0.0
6	1.2937694328211838E-4	0.0	0.0	0.0	0.0
7	1.61721179102648E-4	0.0	0.0	0.0	0.0
8	1.61721179102648E-4	0.0	0.0	0.0	0.0
9	1.61721179102648E-4	0.0	0.0	0.0	0.0
10	1.61721179102648E-4	0.0	0.0	0.0	0.0
11	1.61721179102648E-4	0.0	0.0	0.0	0.0
12	1.61721179102648E-4	0.0	0.0	0.0	0.0
13	1.61721179102648E-4	0.0	0.0	0.0	0.0
14	1.61721179102648E-4	0.0	0.0	0.0	0.0
15	1.940654149231776E-4	0.0	0.0	0.0	0.0
16	1.940654149231776E-4	0.0	0.0	0.0	0.0
17	1.940654149231776E-4	0.0	0.0	0.0	0.0
18	1.940654149231776E-4	0.0	0.0	0.0	0.0
19	2.264096507437072E-4	0.0	0.0	0.0	0.0
20	2.264096507437072E-4	0.0	0.0	0.0	0.0
21	2.264096507437072E-4	0.0	0.0	3.2344235820529595E-5	0.0
22	2.5875388656423676E-4	0.0	0.0	9.70327074615888E-5	0.0
23	2.5875388656423676E-4	0.0	0.0	1.2937694328211838E-4	6.468847164105919E-5
24	3.5578659402582557E-4	0.0	0.0	1.940654149231776E-4	6.468847164105919E-5
25	3.5578659402582557E-4	0.0	0.0	2.5875388656423676E-4	6.468847164105919E-5
26	3.5578659402582557E-4	0.0	0.0	2.910981223847664E-4	6.468847164105919E-5
27	3.5578659402582557E-4	0.0	0.0	3.5578659402582557E-4	6.468847164105919E-5
28	4.8516353730794395E-4	0.0	0.0	5.498520089490032E-4	6.468847164105919E-5
29	5.821962447695328E-4	0.0	0.0	0.0011967367253595952	9.70327074615888E-5
30	5.821962447695328E-4	0.0	0.0	0.0022317522716165422	9.70327074615888E-5
31	5.821962447695328E-4	0.0	0.0	0.004560537250694673	1.2937694328211838E-4
32	5.821962447695328E-4	0.0	0.0	0.007148076116337041	1.2937694328211838E-4
33	5.821962447695328E-4	0.0	0.0	0.010576565113313178	1.2937694328211838E-4
34	5.821962447695328E-4	0.0	0.0	0.01452256188341779	1.61721179102648E-4
35	7.762616596927104E-4	0.0	0.0	0.01937419725649723	1.61721179102648E-4
36	8.409501313337695E-4	0.0	0.0	0.025260848175833617	1.61721179102648E-4
37	8.409501313337695E-4	0.0	0.0	0.03237658005635013	1.61721179102648E-4
38	9.379828387953583E-4	0.0	0.0	0.04075373713386729	1.61721179102648E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2085	0.0	23.952612	1
GTATCAA	4465	0.0	23.552635	1
ATCAACG	5850	0.0	17.599566	3
TCAACGC	5920	0.0	17.392027	4
CAACGCA	5940	0.0	17.296429	5
TATCAAC	6040	0.0	17.228333	2
AACGCAG	6115	0.0	16.691614	6
CGCGATC	95	3.345335E-4	16.212488	23
GTACATG	7615	0.0	15.254469	1
TACATGG	7655	0.0	14.886876	2
ACGCAGA	6995	0.0	14.403287	7
CGCACGA	170	7.6021024E-7	14.235174	35
TTAGTCG	125	1.676613E-4	14.075779	39
CGCAGAG	7195	0.0	13.942222	8
GTGCCGA	300	0.0	13.933892	11
GTATAGA	570	0.0	13.89507	1
ACATGGG	7970	0.0	13.801416	3
TAATACT	750	0.0	13.786773	4
GTATTAG	470	0.0	13.574793	1
AGAGTAC	6825	0.0	13.410062	11
>>END_MODULE