FastQCFastQC Report
Mon 6 Feb 2017
SRR2077453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091741
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.10353389886151525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.024.4387151
GTATCAA51550.023.0067751
CGTGCCG1751.9099389E-1017.60011110
TATCAAC71650.016.398862
CAACGCA72150.016.1335165
TCAACGC72600.016.1244454
ATCAACG72900.016.1181953
CGCGAAT1004.9453403E-415.40059528
AACGCAG75600.015.3945266
GTGCCGA2601.6370905E-1114.38563611
CGAATCG2950.014.16705937
CGCGATC1302.330948E-413.53942223
GTACATG69000.013.2978881
CGTACAC2653.4924597E-1013.2854663
GACCGTA1000.008764178513.2000827
CGCCAAT3350.013.1299542
ACGCAGA88800.012.8829637
TACATGG69600.012.8668972
TACCGAT3501.8189894E-1212.5715077
ACCGTGC5600.012.57150658
Produced by FastQC (version 0.11.5)