FastQCFastQC Report
Mon 6 Feb 2017
SRR2077452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377283
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35150.025.0990871
GGTATCA15150.024.396951
ACGCGTA550.004493737619.9931739
TCAACGC44650.019.4139394
TATCAAC45700.019.1592432
CAACGCA45300.019.0851975
ATCAACG45700.019.0148263
GGACCGT2600.018.614126
AACGCAG47200.018.363166
GTATATA6500.015.9082861
GAGTACT26850.015.89697912
ACGCAGA55800.015.2967747
TTATACT6200.015.2586354
TATACTG5400.015.0733685
GTACTTT28550.014.95039914
ATTATAC5750.014.9213543
AGTACTT29350.014.84274613
CGCAGAG57300.014.7433848
GTACATG62850.014.3521661
AGAGTAC56000.013.94724311
Produced by FastQC (version 0.11.5)