FastQCFastQC Report
Mon 6 Feb 2017
SRR2077452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377283
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.025.2165991
GTATCAA41700.024.5380061
ACGTCGT550.004484368520.00038323
TATCAAC57700.017.5419142
ATCAACG59850.017.0955773
TCAACGC60200.017.0327344
CAACGCA59750.017.0140695
ACCGTAT1702.4701876E-916.8238538
AACGCAG62900.016.2649066
GTACGTA850.0029794415.53068911
GTACATG62150.014.4811991
CGCTACG3400.014.23556728
ACGCAGA72900.013.9728327
TACATGG62450.013.95268252
CGCGCAA950.00624051513.89529521
TATCTCG3350.013.78927836
TATACTG6550.013.7735735
CGCTATC3400.013.58735233
TAACGTC1302.3366089E-413.53530344
GAGTACT43950.013.5164712
Produced by FastQC (version 0.11.5)