##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2377283
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.582498171231613	31.0	31.0	34.0	27.0	34.0
2	30.518957566263673	31.0	31.0	34.0	27.0	34.0
3	30.697516450502526	31.0	31.0	34.0	27.0	34.0
4	34.194180919983026	37.0	35.0	37.0	30.0	37.0
5	33.84027480110698	37.0	35.0	37.0	28.0	37.0
6	33.79151493532743	37.0	35.0	37.0	29.0	37.0
7	33.60918788381526	37.0	35.0	37.0	28.0	37.0
8	33.567019997198486	37.0	35.0	37.0	28.0	37.0
9	35.02263298059171	39.0	35.0	39.0	28.0	39.0
10	34.876577168136905	39.0	35.0	39.0	27.0	39.0
11	34.848793770030746	39.0	35.0	39.0	27.0	39.0
12	34.773869581366625	39.0	35.0	39.0	27.0	39.0
13	34.750202226659596	39.0	35.0	39.0	27.0	39.0
14	35.9071313764495	40.0	36.0	41.0	27.0	41.0
15	35.908544754663204	40.0	36.0	41.0	27.0	41.0
16	35.84681798506951	40.0	36.0	41.0	27.0	41.0
17	35.81720687019593	40.0	36.0	41.0	27.0	41.0
18	35.80832277856696	40.0	36.0	41.0	27.0	41.0
19	35.8449843792262	40.0	36.0	41.0	27.0	41.0
20	35.83789813833692	40.0	36.0	41.0	27.0	41.0
21	35.77721331452755	40.0	36.0	41.0	27.0	41.0
22	35.78018687720393	40.0	36.0	41.0	27.0	41.0
23	35.71606746020562	40.0	36.0	41.0	27.0	41.0
24	35.70062377933128	40.0	36.0	41.0	27.0	41.0
25	35.68521248837433	40.0	36.0	41.0	27.0	41.0
26	35.601974607146055	40.0	36.0	41.0	26.0	41.0
27	35.54449932969697	40.0	35.0	41.0	26.0	41.0
28	35.478762520070184	40.0	35.0	41.0	26.0	41.0
29	35.39079360766051	40.0	35.0	41.0	25.0	41.0
30	35.267017431243985	39.0	35.0	41.0	25.0	41.0
31	35.1831481569506	39.0	35.0	41.0	25.0	41.0
32	35.09917119669808	39.0	35.0	41.0	24.0	41.0
33	35.00451734185623	39.0	35.0	41.0	24.0	41.0
34	34.97749784102271	39.0	35.0	41.0	24.0	41.0
35	34.91255311210319	39.0	35.0	41.0	24.0	41.0
36	34.80383025495913	39.0	34.0	41.0	24.0	41.0
37	34.71700929169981	39.0	34.0	41.0	23.0	41.0
38	34.6268096814725	39.0	34.0	41.0	23.0	41.0
39	34.53898084493937	39.0	34.0	41.0	22.0	41.0
40	34.44474259059607	39.0	34.0	41.0	21.0	41.0
41	34.38346549401144	39.0	34.0	41.0	20.0	41.0
42	34.27304910690061	39.0	34.0	41.0	19.0	41.0
43	34.367656269783616	39.0	34.0	41.0	19.0	41.0
44	34.30779928178513	39.0	34.0	41.0	18.0	41.0
45	34.23807094064947	39.0	34.0	41.0	16.0	41.0
46	34.09170763430353	39.0	34.0	41.0	15.0	41.0
47	33.943865749260816	39.0	34.0	41.0	9.0	41.0
48	33.79610336674262	39.0	33.0	41.0	2.0	41.0
49	33.642110341932366	39.0	33.0	41.0	2.0	41.0
50	33.49213829401043	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6854617678963315
1101	2	0.8460156393278133
1101	3	0.7185104322137299
1101	4	0.7102697821216708
1101	5	0.8563104575050602
1101	6	0.8064856106675506
1101	7	0.8147843287852155
1101	8	0.8440101140203922
1101	9	0.9265728385657965
1101	10	0.9790357594231907
1101	11	1.083909289999319
1101	12	1.055647714268261
1101	13	0.9972866787305392
1101	14	1.1271173304556612
1101	15	1.0933217980201206
1101	16	1.1396995586007321
1101	17	1.185735849195396
1101	18	1.1813284800913593
1101	19	1.1567801308774577
1101	20	1.1862360900104747
1101	21	1.2713197541069832
1101	22	1.3090734494534004
1101	23	1.275148345039696
1101	24	1.2518257910240607
1101	25	1.1846727616591224
1101	26	1.123992618530707
1101	27	1.2270989470105604
1101	28	1.0986297592443748
1101	29	1.1972454231865655
1101	30	1.3114310086005219
1101	31	1.2395181513767213
1101	32	1.2637392574411948
1101	33	1.2445915026283103
1101	34	1.2131724982672552
1101	35	1.194696142958584
1101	36	1.364575822874727
1101	37	1.3084459179085073
1101	38	1.322184032309984
1101	39	1.2404260585154248
1101	40	1.3183147496364995
1101	41	1.2545766528404414
1101	42	1.3259736155878912
1101	43	1.4365274263376264
1101	44	1.4615264910415249
1101	45	1.461070184067161
1101	46	1.4388314959361708
1101	47	1.5130986453570259
1101	48	1.4847670745968031
1101	49	1.4733013360774478
1101	50	1.5930148035508722
1102	1	0.6381501934676024
1102	2	0.7690938912096499
1102	3	0.6692315387976997
1102	4	0.6896225549345161
1102	5	0.6903642294259171
1102	6	0.6490037183591397
1102	7	0.7077291725585297
1102	8	0.7306492481212175
1102	9	0.6396509639197561
1102	10	0.7222879030288354
1102	11	0.842107405825054
1102	12	0.8569886521034533
1102	13	0.7927726340231658
1102	14	0.9378825490499807
1102	15	0.9586202261272518
1102	16	0.9800076713935866
1102	17	0.9701711481076458
1102	18	0.961156876476359
1102	19	1.0201435917344739
1102	20	0.9244564398895889
1102	21	1.0567766708742994
1102	22	0.9602920227145191
1102	23	0.9424983884872447
1102	24	0.9176073434573979
1102	25	0.957316875199929
1102	26	0.8790939836452125
1102	27	0.9180401499633177
1102	28	0.9607361240281733
1102	29	1.003518371989827
1102	30	0.9574021603973719
1102	31	1.1401695274658152
1102	32	0.987166680788981
1102	33	1.0733039042462593
1102	34	1.0642790451148016
1102	35	0.9316203019251716
1102	36	1.0477607157802424
1102	37	1.051340449805764
1102	38	1.0432654622385797
1102	39	1.054533582809725
1102	40	1.0771872514402787
1102	41	1.0502741445504
1102	42	1.0846180641623846
1102	43	1.0074926286727361
1102	44	0.9333201327204961
1102	45	0.8609428076256052
1102	46	0.9191054598414823
1102	47	1.0540147662428154
1102	48	1.1751053381479935
1102	49	1.2110206507388526
1102	50	1.112218287113194
1103	1	0.4762184248531476
1103	2	0.6500829689416179
1103	3	0.5615377142651354
1103	4	0.5341368136263327
1103	5	0.5574266483166141
1103	6	0.5717836022295657
1103	7	0.5674269270379284
1103	8	0.6161338944350305
1103	9	0.6914989474154254
1103	10	0.7260481106927159
1103	11	0.7001325112784258
1103	12	0.6776348161035273
1103	13	0.6253954493740252
1103	14	0.8041322956069195
1103	15	0.8119500919784102
1103	16	0.7667994070739823
1103	17	0.6963951051405459
1103	18	0.7075309109109256
1103	19	0.705789584265645
1103	20	0.8095094809356311
1103	21	0.8205567538893135
1103	22	0.845520819381882
1103	23	0.8901684119333595
1103	24	0.9097881360528319
1103	25	0.8607490508274012
1103	26	0.8145765542828798
1103	27	0.865936993672122
1103	28	0.9207708600172069
1103	29	0.9862291930009661
1103	30	0.9882540780513906
1103	31	0.9311915813744349
1103	32	0.9599184678258084
1103	33	0.8379561472700345
1103	34	0.9015892034738542
1103	35	1.0467959612832516
1103	36	1.0682845652991375
1103	37	0.9987324379365106
1103	38	0.8949232055033107
1103	39	0.918337190154368
1103	40	0.9550256633615177
1103	41	0.9520499155738449
1103	42	0.9033165520473574
1103	43	0.9363637195578747
1103	44	0.8169581041170773
1103	45	0.7424942732635387
1103	46	1.011226975337756
1103	47	0.8242454641092039
1103	48	0.8719226798506128
1103	49	0.7949403876514154
1103	50	0.9403295902828432
1104	1	0.6013080618360114
1104	2	0.7071561377138487
1104	3	0.5527872850490425
1104	4	0.6094909429380593
1104	5	0.6857152469684777
1104	6	0.6586558978706378
1104	7	0.7350569371129723
1104	8	0.62868217938572
1104	9	0.6896380687736325
1104	10	0.6810342031986778
1104	11	0.7598405610216687
1104	12	0.8355938511840932
1104	13	0.893151382672805
1104	14	0.8520340016999697
1104	15	0.8318429560711849
1104	16	0.8832958700953384
1104	17	0.9591780827725103
1104	18	0.953810195052867
1104	19	0.8307854185635364
1104	20	0.9298978999267078
1104	21	0.8721467428740084
1104	22	0.958758438766445
1104	23	1.017577675502686
1104	24	0.9898418468305152
1104	25	1.0224861545575479
1104	26	0.9017522023024753
1104	27	0.9061087187905343
1104	28	0.9049623924540384
1104	29	0.9449985612841374
1104	30	0.987901629898019
1104	31	0.9210559074322688
1104	32	0.9582274903200059
1104	33	0.9063162859046017
1104	34	0.8541426003361252
1104	35	0.9426316874157123
1104	36	1.0019053667921298
1104	37	1.0912700884448157
1104	38	1.0557817924138462
1104	39	1.0199893968755518
1104	40	0.99471282805969
1104	41	0.9167226044571919
1104	42	1.028758265245905
1104	43	0.9485216187109842
1104	44	0.9889893272445818
1104	45	0.8178883486829847
1104	46	0.8515425213214556
1104	47	0.9403141987890891
1104	48	0.9766249987779574
1104	49	0.9988686149844881
1104	50	0.9721440987091654
1105	1	0.37768347714240846
1105	2	0.6308411407682861
1105	3	0.4178050988191089
1105	4	0.4009001835133077
1105	5	0.5538583384443783
1105	6	0.5832332095731374
1105	7	0.49562823215681107
1105	8	0.44739082053722967
1105	9	0.5478489657252084
1105	10	0.554856665286735
1105	11	0.5924160018481359
1105	12	0.7006933798374533
1105	13	0.7343454717593048
1105	14	0.7141677344753177
1105	15	0.7592348501484381
1105	16	0.8177471256031836
1105	17	0.7976163616291316
1105	18	0.8166745944555558
1105	19	0.8144480286114018
1105	20	0.837494754596456
1105	21	0.8320411228491054
1105	22	0.7173384084000887
1105	23	0.808267571840986
1105	24	0.7802373610186208
1105	25	0.7289190674949921
1105	26	0.7285763157462739
1105	27	0.7826648677535601
1105	28	0.8084551040749162
1105	29	0.9245503987256001
1105	30	0.8965698657586003
1105	31	0.9384608796153131
1105	32	0.9276779677859537
1105	33	0.8818345217689156
1105	34	0.8814291142443835
1105	35	0.9431982538717776
1105	36	0.9870202223865832
1105	37	0.9914766997226465
1105	38	1.0029772921808018
1105	39	0.8973008684607819
1105	40	1.0412818942313962
1105	41	0.92730121808561
1105	42	1.0251716746210064
1105	43	0.9816934664513539
1105	44	1.0107628798836785
1105	45	1.0152816364571038
1105	46	0.9371415804308114
1105	47	1.0033363369673935
1105	48	0.9328198621818871
1105	49	1.014107733068606
1105	50	1.051024510951443
1106	1	0.37694553643881434
1106	2	0.4713059225097389
1106	3	0.37100576549368824
1106	4	0.36258244211744994
1106	5	0.394413686635005
1106	6	0.27441568646001
1106	7	0.3496225862619582
1106	8	0.3980835439626773
1106	9	0.3921527097685953
1106	10	0.4401004011439653
1106	11	0.4342889599813802
1106	12	0.38767660701899587
1106	13	0.5092544326939503
1106	14	0.5015517841409007
1106	15	0.515534603992343
1106	16	0.5326191037722694
1106	17	0.6078545909583255
1106	18	0.5706312711592716
1106	19	0.5630724957091786
1106	20	0.543041466001192
1106	21	0.5749594640058859
1106	22	0.628479972113368
1106	23	0.6491645226093326
1106	24	0.46720761873292815
1106	25	0.43001197592220564
1106	26	0.4641176679033592
1106	27	0.41265585946936767
1106	28	0.43100711713407236
1106	29	0.4643736974282575
1106	30	0.662229618160481
1106	31	0.5625828576744922
1106	32	0.6927288146921668
1106	33	0.6903159378412909
1106	34	0.5628822924892631
1106	35	0.5911666857488811
1106	36	0.6312637793091369
1106	37	0.6716718396456116
1106	38	0.6375423292229243
1106	39	0.6427832073437258
1106	40	0.6413518853901721
1106	41	0.5309859536381722
1106	42	0.6519398636393419
1106	43	0.6376526288963476
1106	44	0.5479553551306822
1106	45	0.504097998912826
1106	46	0.671892234729448
1106	47	0.5084838965313168
1106	48	0.45573647114541416
1106	49	0.5689501144738145
1106	50	0.5949006582546943
1107	1	0.41465678807994166
1107	2	0.5893547899968077
1107	3	0.4288820002346867
1107	4	0.5646218463905086
1107	5	0.4628726482170151
1107	6	0.35077161848130345
1107	7	0.4007241568030864
1107	8	0.39939472770245743
1107	9	0.44736618002200146
1107	10	0.41491437077315396
1107	11	0.5054639757211703
1107	12	0.46088737411090364
1107	13	0.5169431866396437
1107	14	0.5776470695092328
1107	15	0.4676718850588273
1107	16	0.5677204508405893
1107	17	0.5645532958904127
1107	18	0.5987649938509989
1107	19	0.5143975390607594
1107	20	0.6078621183269206
1107	21	0.5179369383763515
1107	22	0.45823967437882374
1107	23	0.6355273092293245
1107	24	0.5522085104753955
1107	25	0.5690537502963195
1107	26	0.5203844686537096
1107	27	0.6035057222699578
1107	28	0.5921026496117605
1107	29	0.6479134207947084
1107	30	0.7352626813925411
1107	31	0.7870054275688361
1107	32	0.735398799070559
1107	33	0.7489711042533287
1107	34	0.7261008166662322
1107	35	0.680722769903582
1107	36	0.7301315886954214
1107	37	0.8111964442169182
1107	38	0.7341236249784444
1107	39	0.7521516602887388
1107	40	0.683443418993825
1107	41	0.7938299459799296
1107	42	0.742644469113138
1107	43	0.6823656704687338
1107	44	0.7162396211406801
1107	45	0.6142058460316377
1107	46	0.730069083376037
1107	47	0.7377661405421421
1107	48	0.8211321362026496
1107	49	0.7897345899895782
1107	50	0.9574848634543045
1108	1	0.26621478560677403
1108	2	0.41704429093842776
1108	3	0.290729106172563
1108	4	0.3277614102958424
1108	5	0.26099380516117776
1108	6	0.29629091317419665
1108	7	0.2960521495650923
1108	8	0.3102395902033024
1108	9	0.4365164250699891
1108	10	0.3938127325819707
1108	11	0.4455115955791342
1108	12	0.47305759454064855
1108	13	0.421836984996915
1108	14	0.4402696644086106
1108	15	0.5656895914777209
1108	16	0.3922647438317597
1108	17	0.502165396836574
1108	18	0.4714064069610657
1108	19	0.48595708231853507
1108	20	0.4784232470224907
1108	21	0.5310158784303525
1108	22	0.4466478267623657
1108	23	0.4954330338226143
1108	24	0.3498721168784087
1108	25	0.3380982191321138
1108	26	0.4392282760868298
1108	27	0.42224969533736356
1108	28	0.31869347619493027
1108	29	0.4699534169374715
1108	30	0.5684281823795416
1108	31	0.4913173787634477
1108	32	0.4797917664201776
1108	33	0.43122980005347955
1108	34	0.35670246052981724
1108	35	0.47917030297371355
1108	36	0.5004103024203772
1108	37	0.36546358339509766
1108	38	0.43011089326061125
1108	39	0.47932412131930136
1108	40	0.46850130590871686
1108	41	0.44028610961753145
1108	42	0.42173994707016504
1108	43	0.47953804641318953
1108	44	0.5465794834146394
1108	45	0.4994540422061675
1108	46	0.4888908109643566
1108	47	0.350818916618195
1108	48	0.37217302291333
1108	49	0.42435789961224657
1108	50	0.49289004349650867
1109	1	0.19298869231658244
1109	2	0.2850976479837506
1109	3	0.2129930077674338
1109	4	0.15508487727856846
1109	5	0.13641784380038047
1109	6	0.12938720642022616
1109	7	0.15154756921547374
1109	8	0.12097309114292187
1109	9	0.15993493416647198
1109	10	0.17364697023062803
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2211	14	-0.2136806499708328
2211	15	-0.23679967861522755
2211	16	-0.09251498552854542
2211	17	-0.2293018666954083
2211	18	-0.27667068434566033
2211	19	-0.16563873506801485
2211	20	-0.2297244429720422
2211	21	-0.13720042829463353
2211	22	-0.22019881074446346
2211	23	-0.17190266874709437
2211	24	-0.13378432106582494
2211	25	-0.12448099081868236
2211	26	-0.2425008791892722
2211	27	-0.0967566605948349
2211	28	-0.10574802189145771
2211	29	-0.11114103358194427
2211	30	-0.09593410575605077
2211	31	-0.22897902637597412
2211	32	-0.23948775791005517
2211	33	-0.14267807310125136
2211	34	-0.0889975121328348
2211	35	-0.0891258287922767
2211	36	-0.10784707866218213
2211	37	-0.04867590539659261
2211	38	-0.08436443478750277
2211	39	-0.001456838221436385
2211	40	-0.005532182336231983
2211	41	0.1030326924496805
2211	42	-0.13206814478331097
2211	43	-0.19361101044847828
2211	44	-0.051547225169287
2211	45	-0.09277381454615607
2211	46	-0.10735959909578696
2211	47	-0.043393767908867176
2211	48	-0.06384501973292345
2211	49	-0.07615201017451767
2211	50	-0.04368212708850905
2212	1	-0.8163930971574231
2212	2	-2.3113807887875524
2212	3	-1.1376001348633764
2212	4	-0.6613324846475734
2212	5	-0.5531553698680085
2212	6	-0.5684034109493865
2212	7	-0.6102585392809914
2212	8	-0.5245215754086772
2212	9	-0.5368604073782564
2212	10	-0.5703068859144551
2212	11	-0.4330503783089057
2212	12	-0.5024247504706878
2212	13	-1.899570505043016
2212	14	-1.3802930085013685
2212	15	-0.9757877212570847
2212	16	-0.7958250381824215
2212	17	-0.8033052732023549
2212	18	-0.7058311446563934
2212	19	-0.6706992135352579
2212	20	-0.666934773161131
2212	21	-0.6573155232704693
2212	22	-0.6165778840708285
2212	23	-0.6640263059281679
2212	24	-0.5793975528379889
2212	25	-0.7233653857313982
2212	26	-0.7638734421102242
2212	27	-0.8385931655532914
2212	28	-0.757606954461636
2212	29	-0.7323880226275108
2212	30	-0.6809804740219292
2212	31	-0.5748666116467192
2212	32	-0.5689877276492084
2212	33	-0.5973156778253994
2212	34	-0.6301536840490414
2212	35	-0.6944186068246267
2212	36	-0.5756602914132358
2212	37	-0.5106858136629882
2212	38	-0.6517350783060607
2212	39	-0.6093888464178008
2212	40	-0.5774917538426152
2212	41	-0.5361938748461199
2212	42	-0.4264598584983901
2212	43	-0.5078827528681416
2212	44	-0.5300583914223935
2212	45	-0.5111106437279886
2212	46	-0.5357345223629153
2212	47	-0.5898289534943331
2212	48	-0.44676780269347205
2212	49	-0.6027277098485655
2212	50	-0.5713850520156711
2213	1	-0.7473006729852258
2213	2	-1.85855665528306
2213	3	-1.045808742686873
2213	4	-0.7172922043703593
2213	5	-0.6149204432322009
2213	6	-0.6262599593157461
2213	7	-0.5490436217942971
2213	8	-0.6025822457121492
2213	9	-0.5483134234617495
2213	10	-0.6135137707222924
2213	11	-0.6382368569687031
2213	12	-0.517515747865005
2213	13	-0.7828110037703979
2213	14	-0.7563230393544345
2213	15	-0.7194941798452561
2213	16	-0.7922601087051504
2213	17	-0.7603180477009204
2213	18	-0.7561578123038046
2213	19	-0.7638352632548404
2213	20	-0.944026927293443
2213	21	-0.8636108783829926
2213	22	-0.946069362700392
2213	23	-0.9177071107276262
2213	24	-0.8146849135608889
2213	25	-0.9299509329551299
2213	26	-0.8976625852978657
2213	27	-0.9476610644616486
2213	28	-0.9418280606981497
2213	29	-0.9481493839509483
2213	30	-0.9843959747141611
2213	31	-0.8546158372972883
2213	32	-0.6633208198275184
2213	33	-0.7579405380193052
2213	34	-0.8169336196828212
2213	35	-0.8317757023240304
2213	36	-0.8674116814132944
2213	37	-0.7509255848948726
2213	38	-0.7917240550362834
2213	39	-0.7929600432385868
2213	40	-0.8307241715257945
2213	41	-0.8775252923741306
2213	42	-0.9544024367343837
2213	43	-0.9813014577649284
2213	44	-1.01652868804306
2213	45	-1.0118759246601527
2213	46	-0.9369452683380146
2213	47	-0.9798647841591688
2213	48	-0.9984759057036072
2213	49	-0.9520111973128706
2213	50	-1.0452239309068148
2214	1	-0.8482258295423897
2214	2	-1.9504774951184949
2214	3	-0.8997312848914412
2214	4	-0.585909840103902
2214	5	-0.5278290624892392
2214	6	-0.4159433997226927
2214	7	-0.4709342648439687
2214	8	-0.3822288014546089
2214	9	-0.545473101632922
2214	10	-0.5616026587104628
2214	11	-0.4886685291410444
2214	12	-0.5287311178262968
2214	13	-0.6018785513455356
2214	14	-0.5762519046443444
2214	15	-0.7296121297064317
2214	16	-0.7508580861284955
2214	17	-0.6989243343069234
2214	18	-0.6816396435665979
2214	19	-0.7053396306423139
2214	20	-0.6458225214000066
2214	21	-0.7335235306335619
2214	22	-0.6430142181003475
2214	23	-0.6769971822676624
2214	24	-0.6070607318127159
2214	25	-0.7128461764547112
2214	26	-0.7171597784154287
2214	27	-0.7613712158509003
2214	28	-0.7026522910854354
2214	29	-0.7627076799605135
2214	30	-0.7243114642466182
2214	31	-0.8048823391313888
2214	32	-0.6872867319123159
2214	33	-0.6912512406519014
2214	34	-0.6586457166367978
2214	35	-0.722061051816361
2214	36	-0.7445138032948222
2214	37	-0.8225345263860717
2214	38	-0.880025333839761
2214	39	-0.8678409787309533
2214	40	-0.8543415032648412
2214	41	-0.8866937661191372
2214	42	-0.8062912558171291
2214	43	-0.7763554674522197
2214	44	-0.6685613978499276
2214	45	-0.7495337484649482
2214	46	-0.6802969088949311
2214	47	-0.61179129855838
2214	48	-0.8588117182808759
2214	49	-0.7992762602397292
2214	50	-0.8710421827358488
2215	1	-0.43180335030081807
2215	2	-0.40228979576542656
2215	3	-0.6467506841860917
2215	4	-0.509544240319876
2215	5	-0.6162727622536934
2215	6	-0.6607387579475699
2215	7	-0.678298026258787
2215	8	-0.7454689878912433
2215	9	-0.8467629597485242
2215	10	-0.9513045057929972
2215	11	-0.9843820006591812
2215	12	-0.9213606428642578
2215	13	-0.9214848232270612
2215	14	-1.0158278370060643
2215	15	-0.9780512299768347
2215	16	-0.9131260937905097
2215	17	-1.0493356462054706
2215	18	-1.1662403374902652
2215	19	-1.0268555496055143
2215	20	-1.1825180563197364
2215	21	-1.0856792220789302
2215	22	-1.0969651406417285
2215	23	-0.9822027525120731
2215	24	-1.0276890341280307
2215	25	-1.0847872629352011
2215	26	-1.0718860442404718
2215	27	-1.0769813558783525
2215	28	-1.1838052214059616
2215	29	-1.1869910087943438
2215	30	-1.095412643116937
2215	31	-1.217655505511658
2215	32	-1.0461341158429391
2215	33	-1.2381219420176066
2215	34	-1.1696175097395525
2215	35	-1.1671543772648434
2215	36	-1.185444015257218
2215	37	-1.164176802878515
2215	38	-1.174268395674389
2215	39	-1.0657207833810176
2215	40	-1.1121040065323413
2215	41	-1.1634146125403149
2215	42	-1.2254794308321664
2215	43	-1.160449788505474
2215	44	-1.0730040520151078
2215	45	-1.0006804080466765
2215	46	-0.9339600059542548
2215	47	-0.9881854666284582
2215	48	-0.9364293232014802
2215	49	-0.9020515263890232
2215	50	-0.8499917839939926
2216	1	-1.6000036361481627
2216	2	-1.193235822005569
2216	3	-1.2862136765902612
2216	4	-2.8434396330387983
2216	5	-2.1810220034735224
2216	6	-1.8585354851560645
2216	7	-1.7029081979277834
2216	8	-1.703145916156405
2216	9	-1.8088078923816937
2216	10	-1.8182650492241237
2216	11	-1.9035016550729509
2216	12	-1.9041134491464717
2216	13	-1.9598574204231838
2216	14	-2.18330547507194
2216	15	-2.1381016547331413
2216	16	-2.232432030743503
2216	17	-2.0096622830026973
2216	18	-2.1091511627540314
2216	19	-2.142904753890015
2216	20	-2.307809122787269
2216	21	-2.2076942852704633
2216	22	-2.1077325925856485
2216	23	-2.180585627365538
2216	24	-2.320875268490738
2216	25	-2.289296585091577
2216	26	-2.4793148634339985
2216	27	-2.532915900048195
2216	28	-2.394071637554731
2216	29	-2.2847090476888567
2216	30	-2.3702918003490367
2216	31	-2.1489236720739413
2216	32	-2.088264481502719
2216	33	-2.0777904875685067
2216	34	-2.1732949840052598
2216	35	-2.307821782638136
2216	36	-2.366467284515778
2216	37	-2.4048506317876317
2216	38	-2.5425939404705176
2216	39	-2.714345343770283
2216	40	-2.690712026316831
2216	41	-2.6052093484527
2216	42	-2.5774329886680754
2216	43	-2.411248715774992
2216	44	-2.223828673427967
2216	45	-2.273873387122009
2216	46	-2.296905839488325
2216	47	-2.316299677095614
2216	48	-2.3314446593932843
2216	49	-2.391500868779545
2216	50	-2.4641460452070802
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55491.0
3	8660.0
4	15100.0
5	8907.0
6	6817.0
7	5260.0
8	4553.0
9	4021.0
10	4081.0
11	3783.0
12	4093.0
13	4222.0
14	4633.0
15	4921.0
16	5556.0
17	5803.0
18	6458.0
19	7286.0
20	8296.0
21	10485.0
22	13124.0
23	15826.0
24	18621.0
25	21198.0
26	23432.0
27	26667.0
28	31426.0
29	36460.0
30	41999.0
31	50590.0
32	61199.0
33	75707.0
34	96034.0
35	121324.0
36	157553.0
37	212052.0
38	335413.0
39	860232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.276650169043144	18.558942962874458	14.05855842969101	26.105848438391387
2	14.796877924842203	20.293152004509384	38.397402056565	26.51256801408341
3	17.377761250911757	24.999432122674964	29.866687643073536	27.75611898333974
4	12.212317608175226	16.904457328715637	37.204966618725734	33.6782584443834
5	13.59420960717066	37.81787194647253	34.64636943211227	13.941549014244533
6	31.97352559395953	37.29457825875661	17.407174429392818	13.324721717891052
7	27.838397354170084	32.16509507399319	22.0187917881653	17.977715783671425
8	26.586915736853907	34.422545569906255	20.034896641734683	18.955642051505155
9	26.61263922375524	15.180028360199785	19.13598168789474	39.071350728150236
10	14.167589575450739	28.808176915723	34.17891694830813	22.845316560518132
11	34.95064574811131	22.716758311237502	23.88457959990591	18.448016340745273
12	23.348978992399356	24.744001164797858	30.53335790854822	21.373661934254567
13	28.03097758484583	21.705307293130698	26.25343582889951	24.010279293123965
14	22.137006569811188	20.87640405937911	27.475882918322586	29.510706452487117
15	23.679057947870525	28.83086345100148	23.835258701185687	23.654819899942307
16	23.463141961029216	27.87581329406769	25.45784547612605	23.20319926877704
17	22.420756410628684	27.58696584406769	26.876392162605857	23.115885582697775
18	22.413798908167198	27.061495134223385	27.89766634626553	22.627039611343882
19	23.331087396676043	26.672174191536453	27.886663386841615	22.11007502494589
20	23.53479595276401	26.294072271349812	27.62937566465326	22.541756111232917
21	23.775283811627187	26.03365675963209	27.631401753370056	22.559657675370673
22	23.351076879958153	25.93517636726238	27.545562086351556	23.168184666427916
23	23.167568845966578	25.91336974594356	27.52151153713594	23.397549870953924
24	22.93706040628832	26.167403513467335	27.58866358461871	23.30687249562563
25	23.040413132405956	26.148416436589923	27.667406867838114	23.143763563166008
26	23.09662720489095	26.22155882149957	27.65020359313692	23.031610380472564
27	23.045818018999174	26.534372141055435	27.32004272162686	23.09976711831854
28	23.07263721789482	26.234571797688034	27.51983099990321	23.172959984513934
29	23.242624994161265	26.318323191117887	27.53042349027151	22.90862832444934
30	23.252855643011486	26.28138366923564	27.301356864378786	23.164403823374087
31	23.328680756806026	26.007622258475944	27.43449187678653	23.2292051079315
32	23.068714630813968	26.458896740175526	27.274957406547923	23.19743122246258
33	23.179501765464224	26.178645119812582	27.356364591082134	23.285488523641064
34	23.407948812020155	26.224667705595067	27.402255461947284	22.965128020437493
35	23.42274000959281	26.245592776782033	27.223975294305742	23.107691919319414
36	23.3281006823203	26.25256495969484	27.226082079551723	23.193252278433132
37	23.357889643856364	25.99099648694701	27.41658076866441	23.234533100532218
38	23.3260953802618	26.140356265485483	27.245571083753394	23.28797727049932
39	23.389967416295217	26.384470888174157	27.16437627132192	23.061185424208706
40	23.62213005549215	26.085643262590064	27.38542412938829	22.9068025525295
41	23.260539241669317	26.169905044600263	27.152944997215112	23.416610716515308
42	23.50399305380274	26.246335914599467	27.0443997967295	23.205271234868295
43	23.218727428983193	25.96285828104184	27.25651275713344	23.561901532841524
44	23.30831369187014	26.32194443965356	26.940235125456475	23.429506743019832
45	23.440395318735614	26.230586049845595	26.901453419770395	23.42756521164839
46	23.592052006222307	26.15576627487008	26.77172906218688	23.480452656720736
47	23.443069667190237	26.33255569193959	26.706597556697293	23.51777708417288
48	23.427322214963652	26.46989168654594	26.656618713842406	23.446167384648
49	23.50625379110058	26.21233191911085	26.745883316970083	23.535530972818492
50	23.629870334731994	26.32274347730069	26.627630375114364	23.41975581285295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	404.0
1	283.0
2	162.0
3	269.0
4	376.0
5	440.5
6	505.0
7	488.5
8	472.0
9	631.5
10	791.0
11	1134.5
12	1478.0
13	2758.0
14	4038.0
15	5755.0
16	7472.0
17	8929.0
18	10386.0
19	11277.0
20	12168.0
21	13427.0
22	14686.0
23	17719.5
24	20753.0
25	25199.0
26	29645.0
27	35469.0
28	41293.0
29	47394.5
30	53496.0
31	60133.5
32	66771.0
33	74506.5
34	82242.0
35	91048.0
36	99854.0
37	106842.5
38	113831.0
39	121350.0
40	128869.0
41	135492.5
42	142116.0
43	147595.0
44	153074.0
45	158641.0
46	164208.0
47	167536.5
48	170865.0
49	170714.0
50	170563.0
51	167875.0
52	165187.0
53	160491.0
54	155795.0
55	147760.0
56	139725.0
57	129754.0
58	119783.0
59	108089.5
60	96396.0
61	84022.5
62	71649.0
63	60962.5
64	50276.0
65	42066.5
66	33857.0
67	27285.5
68	20714.0
69	17032.5
70	13351.0
71	10581.0
72	7811.0
73	6670.0
74	5529.0
75	4178.0
76	2827.0
77	2245.5
78	1664.0
79	1332.5
80	1001.0
81	763.5
82	526.0
83	438.0
84	350.0
85	266.0
86	182.0
87	125.5
88	69.0
89	53.0
90	37.0
91	29.0
92	21.0
93	13.0
94	5.0
95	5.5
96	6.0
97	4.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015774310420761852
2	7.57166900196569E-4
3	3.785834500982845E-4
4	0.0021873710450123103
5	0.03924648432685549
6	0.02725800840707648
7	0.02999222221334187
8	0.02313565528378405
9	0.02999222221334187
10	0.031085907735848028
11	0.03752182638751886
12	0.03806866914877194
13	0.035965427759337025
14	0.040340169849361646
15	0.035923362931548325
16	0.04164417951081129
17	0.04349503193351402
18	0.046060986428624606
19	0.04795390367911603
20	0.043915680211401
21	0.04454665262823147
22	0.04290612434447224
23	0.04206482778869828
24	0.03958300294916508
25	0.03794247466540584
26	0.04101320709398082
27	0.040760818127248626
28	0.04008778088262946
29	0.038363122943292825
30	0.03567097396481614
31	0.03457728844230998
32	0.03129623187479152
33	0.024019016667346713
34	0.01998079319963168
35	0.021158608377715233
36	0.02271500700589707
37	0.018214070432506353
38	0.019349820782801206
39	0.013797263514693034
40	0.007992317279852673
41	0.007024826240712612
42	0.006520048307248233
43	0.0026921489784766895
44	0.003533445534250655
45	0.002608019322899293
46	0.00121988000587225
47	3.785834500982845E-4
48	6.730372446191724E-4
49	2.1032413894349138E-4
50	3.365186223095862E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2377283.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.40310601887167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03428082113764	45.92706725507838
2	13.21162517153485	15.960463918356261
3	4.6621662162392665	8.448279607113065
4	2.1955529292065656	5.304728654116336
5	1.1386144523830655	3.43879247409569
6	0.6801877516955698	2.465127172704327
7	0.4642568178665458	1.962978765270387
8	0.30150780711945535	1.4569606431163176
9	0.22083901373025996	1.200542612350675
>10	1.0399883964742778	10.748229503058905
>50	0.036532076056085994	1.4949152685027471
>100	0.014028840064108192	1.2925542693053178
>500	1.3990216407186383E-4	0.04533706795926883
>1k	2.7980432813954297E-4	0.2540227889721941
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.2064827788698274E-5
2	0.0	0.0	0.0	0.0	4.2064827788698274E-5
3	0.0	0.0	0.0	0.0	4.2064827788698274E-5
4	0.0	0.0	0.0	0.0	4.2064827788698274E-5
5	4.2064827788698274E-5	0.0	0.0	0.0	4.2064827788698274E-5
6	4.2064827788698274E-5	0.0	0.0	0.0	4.2064827788698274E-5
7	4.2064827788698274E-5	0.0	0.0	0.0	4.2064827788698274E-5
8	4.2064827788698274E-5	0.0	0.0	0.0	4.2064827788698274E-5
9	4.2064827788698274E-5	0.0	0.0	0.0	4.2064827788698274E-5
10	8.412965557739655E-5	0.0	0.0	0.0	4.2064827788698274E-5
11	8.412965557739655E-5	0.0	0.0	0.0	4.2064827788698274E-5
12	1.2619448336609484E-4	0.0	0.0	0.0	4.2064827788698274E-5
13	2.5238896673218967E-4	0.0	0.0	0.0	4.2064827788698274E-5
14	2.5238896673218967E-4	0.0	0.0	0.0	4.2064827788698274E-5
15	2.5238896673218967E-4	0.0	0.0	0.0	8.412965557739655E-5
16	2.5238896673218967E-4	0.0	0.0	0.0	8.412965557739655E-5
17	3.365186223095862E-4	0.0	0.0	0.0	8.412965557739655E-5
18	3.365186223095862E-4	0.0	0.0	0.0	8.412965557739655E-5
19	4.62713105675681E-4	0.0	0.0	0.0	8.412965557739655E-5
20	4.62713105675681E-4	0.0	0.0	4.2064827788698274E-5	8.412965557739655E-5
21	4.62713105675681E-4	0.0	0.0	4.2064827788698274E-5	8.412965557739655E-5
22	4.62713105675681E-4	0.0	0.0	4.2064827788698274E-5	8.412965557739655E-5
23	4.62713105675681E-4	0.0	0.0	1.2619448336609484E-4	8.412965557739655E-5
24	5.468427612530776E-4	0.0	0.0	2.944537945208879E-4	8.412965557739655E-5
25	5.468427612530776E-4	0.0	0.0	3.785834500982845E-4	8.412965557739655E-5
26	5.468427612530776E-4	0.0	0.0	5.047779334643793E-4	8.412965557739655E-5
27	5.468427612530776E-4	0.0	0.0	7.151020724078706E-4	8.412965557739655E-5
28	5.468427612530776E-4	0.0	0.0	0.0010936855225061552	8.412965557739655E-5
29	7.151020724078706E-4	0.0	0.0	0.001977046906068819	8.412965557739655E-5
30	7.151020724078706E-4	0.0	0.0	0.004290612434447224	8.412965557739655E-5
31	7.151020724078706E-4	0.0	0.0	0.008412965557739654	8.412965557739655E-5
32	7.151020724078706E-4	0.0	0.0	0.013250420753439957	8.412965557739655E-5
33	7.151020724078706E-4	0.0	0.0	0.019560144921744696	8.412965557739655E-5
34	7.151020724078706E-4	0.0	0.0	0.026500841506879914	1.682593111547931E-4
35	8.412965557739655E-4	0.0	0.0	0.03373599188653602	1.682593111547931E-4
36	9.674910391400603E-4	0.0	0.0	0.044210134005921886	1.682593111547931E-4
37	9.674910391400603E-4	0.0	0.0	0.05632480440906699	1.682593111547931E-4
38	0.0010516206947174568	0.0	0.0	0.07163640172415316	1.682593111547931E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1850	0.0	25.216599	1
GTATCAA	4170	0.0	24.538006	1
ACGTCGT	55	0.0044843685	20.000383	23
TATCAAC	5770	0.0	17.541914	2
ATCAACG	5985	0.0	17.095577	3
TCAACGC	6020	0.0	17.032734	4
CAACGCA	5975	0.0	17.014069	5
ACCGTAT	170	2.4701876E-9	16.823853	8
AACGCAG	6290	0.0	16.264906	6
GTACGTA	85	0.00297944	15.530689	11
GTACATG	6215	0.0	14.481199	1
CGCTACG	340	0.0	14.235567	28
ACGCAGA	7290	0.0	13.972832	7
TACATGG	6245	0.0	13.9526825	2
CGCGCAA	95	0.006240515	13.895295	21
TATCTCG	335	0.0	13.789278	36
TATACTG	655	0.0	13.773573	5
CGCTATC	340	0.0	13.587352	33
TAACGTC	130	2.3366089E-4	13.535303	44
GAGTACT	4395	0.0	13.51647	12
>>END_MODULE