FastQCFastQC Report
Mon 6 Feb 2017
SRR2077451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559428
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46350.026.1076341
GGTATCA18550.025.6191351
TATCAAC60650.019.6593042
TCAACGC60900.019.4352024
CAACGCA61200.019.411285
GCGATAC2050.019.31767814
ATCAACG61250.019.2865983
GTATACG1051.7934235E-618.8585281
AACGCAG64550.018.4715216
TGCGATA2251.8189894E-1216.62274413
GTACATG88550.015.9266361
ACGCAGA74450.015.8395377
CGCAGAG76500.015.242528
TACATGG90000.015.1791942
GTACTAG4100.014.488871
GTATAGA6950.014.2456521
ACATGGG93550.014.1559873
AGAGTAC76500.014.00566711
GCGTAAC950.006241685713.89517211
TATACTG9050.013.8560085
Produced by FastQC (version 0.11.5)