FastQCFastQC Report
Mon 6 Feb 2017
SRR2077451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559428
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.026.4032971
GTATCAA50550.025.463121
TATCAAC72650.017.5956462
TCAACGC73350.017.2479934
ATCAACG73950.017.1075693
CAACGCA75150.016.7182245
AACGCAG78200.016.2331666
GTACATG83500.015.046191
TATACTG8800.014.7520495
TTATACT9000.014.6682914
CGCGATC1351.95656E-514.66787923
TACATGG85000.014.4696352
TAATACG1251.6708882E-414.0815614
CCGCGAT1751.0529438E-613.83010422
ATACCGT2551.891749E-1013.8042876
ACGCAGA91300.013.6633977
TATACAG10700.013.57197955
ACATGGG89000.013.4483023
CTATACA7450.013.2900634
CGCAGAG94500.013.177448
Produced by FastQC (version 0.11.5)