FastQCFastQC Report
Mon 6 Feb 2017
SRR2077450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739157
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54450.024.1630271
GGTATCA24600.023.7005711
TATCAAC70850.018.3823412
ATCAACG72200.017.8243793
TCAACGC71850.017.8210224
CAACGCA72700.017.7025015
AACGCAG75000.017.0404686
GTATTAC4650.016.086961
GTACATG96750.015.3724761
GTATTAG5050.015.2484151
TACATGG97600.014.9220032
ACGCAGA86150.014.7851187
TCGATCG1351.9647452E-514.66143941
GAGTACT39800.014.37250712
ACATGGG100700.013.9812583
ATACCGT3150.013.9664536
CGCAGAG89400.013.9527158
CGCACGA3550.013.63496435
AGAGTAC85200.013.58277111
TACACCG3450.013.391015
Produced by FastQC (version 0.11.5)