FastQCFastQC Report
Mon 6 Feb 2017
SRR2077450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3739157
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38170.10208183288372219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.026.5417731
GTATCAA59050.024.6310521
TCGATCG809.017601E-519.24370641
CGAATCG3600.017.10780337
TATCAAC84600.016.9832572
ATCAACG85600.016.707743
TCAACGC85800.016.669244
CAACGCA86900.016.3833735
AACGCAG89400.015.9470666
TACCGAT4450.015.8209747
GTACCGA5050.014.8125856
GCCGTAC2855.456968E-1213.8972551
ACCGATC7000.013.82941158
CGATAAC1453.836369E-513.6565510
TGCGATA2754.3655746E-1113.60210113
GTATACG1302.3289764E-413.54091551
GTACATG87500.013.4790141
GAGTACT66150.013.40458412
ACGCAGA106000.013.3874287
CCAGTAC10900.013.3228033
Produced by FastQC (version 0.11.5)