##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3739157
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.38843942631989	31.0	31.0	34.0	26.0	34.0
2	30.341649735488506	31.0	30.0	34.0	26.0	34.0
3	30.50463620543347	31.0	31.0	34.0	26.0	34.0
4	33.9675006960125	37.0	35.0	37.0	30.0	37.0
5	33.561190129218964	37.0	35.0	37.0	28.0	37.0
6	33.493767980322836	37.0	35.0	37.0	28.0	37.0
7	33.30689671495473	37.0	35.0	37.0	27.0	37.0
8	33.269022670083125	37.0	35.0	37.0	26.0	37.0
9	34.714700933927084	39.0	35.0	39.0	27.0	39.0
10	34.57147907937538	39.0	35.0	39.0	27.0	39.0
11	34.514689540984776	39.0	35.0	39.0	26.0	39.0
12	34.434848015207706	39.0	35.0	39.0	26.0	39.0
13	34.3984555877167	39.0	35.0	39.0	26.0	39.0
14	35.542343635209754	40.0	36.0	41.0	26.0	41.0
15	35.542587005573715	40.0	36.0	41.0	26.0	41.0
16	35.48724431736886	40.0	36.0	41.0	25.0	41.0
17	35.45056091520094	40.0	36.0	41.0	25.0	41.0
18	35.444318866525265	40.0	36.0	41.0	25.0	41.0
19	35.47730945772002	40.0	36.0	41.0	25.0	41.0
20	35.47245034107955	40.0	36.0	41.0	25.0	41.0
21	35.410536920487694	40.0	36.0	41.0	25.0	41.0
22	35.408098402928786	40.0	36.0	41.0	25.0	41.0
23	35.33449357702819	40.0	35.0	41.0	25.0	41.0
24	35.31821530895868	40.0	35.0	41.0	25.0	41.0
25	35.293043325006145	40.0	35.0	41.0	25.0	41.0
26	35.21016421615888	40.0	35.0	41.0	24.0	41.0
27	35.15318586515624	40.0	35.0	41.0	24.0	41.0
28	35.08897887946401	40.0	35.0	41.0	24.0	41.0
29	35.007035008158255	40.0	35.0	41.0	24.0	41.0
30	34.88047867473872	39.0	35.0	41.0	23.0	41.0
31	34.802865458711686	39.0	35.0	41.0	23.0	41.0
32	34.72009894208775	39.0	34.0	41.0	22.0	41.0
33	34.618843766121614	39.0	34.0	41.0	21.0	41.0
34	34.59155873904198	39.0	34.0	41.0	21.0	41.0
35	34.52097010101475	39.0	34.0	41.0	20.0	41.0
36	34.41109346304528	39.0	34.0	41.0	18.0	41.0
37	34.32174899315541	39.0	34.0	41.0	18.0	41.0
38	34.23814779641508	39.0	34.0	41.0	17.0	41.0
39	34.145082969236114	39.0	34.0	41.0	16.0	41.0
40	34.05197909582294	39.0	34.0	41.0	15.0	41.0
41	33.98747739129435	39.0	33.0	41.0	15.0	41.0
42	33.86665470318577	39.0	33.0	41.0	14.0	41.0
43	33.969402729010845	39.0	34.0	41.0	13.0	41.0
44	33.907401320672015	39.0	34.0	41.0	9.0	41.0
45	33.83771555995108	39.0	33.0	41.0	8.0	41.0
46	33.68683021333418	39.0	33.0	41.0	2.0	41.0
47	33.53842537234997	39.0	33.0	41.0	2.0	41.0
48	33.38165554428445	39.0	33.0	41.0	2.0	41.0
49	33.224408068449655	39.0	33.0	41.0	2.0	41.0
50	33.075196093664964	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7757048804059998
1101	2	0.9281818658853282
1101	3	0.8166988898667675
1101	4	0.8100812246850211
1101	5	0.7796839686625177
1101	6	0.8762108588160018
1101	7	0.8751929587545177
1101	8	0.9192430023954969
1101	9	0.9071362091669712
1101	10	0.9869706317150246
1101	11	1.0076137360621615
1101	12	0.9358612059958631
1101	13	1.0399503153337761
1101	14	1.118860339611608
1101	15	1.0971618522152298
1101	16	1.044203334846543
1101	17	1.1541318029913157
1101	18	1.1658633639055154
1101	19	1.1165264118698914
1101	20	1.085808282697208
1101	21	1.098483186862154
1101	22	1.1108094287264123
1101	23	1.1933599401227752
1101	24	1.0978474524054462
1101	25	1.0970953677172162
1101	26	1.1986920123104028
1101	27	1.2079825584954307
1101	28	1.2506846945992152
1101	29	1.3036692342669127
1101	30	1.266785031126581
1101	31	1.2633624656505305
1101	32	1.216339519384917
1101	33	1.315440000818576
1101	34	1.24756638544428
1101	35	1.2668630130669882
1101	36	1.2526948842016168
1101	37	1.2409907807523055
1101	38	1.2433297428882213
1101	39	1.218074281265224
1101	40	1.249265880159598
1101	41	1.1991697104600263
1101	42	1.250875478144323
1101	43	1.308790407903203
1101	44	1.2682630682191345
1101	45	1.2762222724340333
1101	46	1.3078799498396947
1101	47	1.3071105256061912
1101	48	1.369996015628459
1101	49	1.2917988434535843
1101	50	1.3605164649918962
1102	1	0.6658310843963662
1102	2	0.7660075882795496
1102	3	0.6752568964027148
1102	4	0.6908130788301818
1102	5	0.7081314660825413
1102	6	0.7065127164011429
1102	7	0.8094894843823113
1102	8	0.8100109762517036
1102	9	0.9036887117469519
1102	10	0.9257154717563054
1102	11	0.9884455644075345
1102	12	0.946075774279322
1102	13	0.8904340614638073
1102	14	0.9908310138462184
1102	15	1.0434810816613904
1102	16	1.1587694683174803
1102	17	1.148738571137173
1102	18	0.9827547037747948
1102	19	1.1583081026163669
1102	20	1.1165154120401724
1102	21	1.1296289126963472
1102	22	1.1105062811515936
1102	23	1.059603060177814
1102	24	1.071744511228978
1102	25	1.046203124855161
1102	26	1.098157741584572
1102	27	1.025673691966368
1102	28	0.9915913423460339
1102	29	1.0723525848001074
1102	30	1.145314227893003
1102	31	1.1685073744912629
1102	32	1.1823826910395425
1102	33	1.1930307300927439
1102	34	1.1593418412406322
1102	35	1.1118675280308636
1102	36	1.0987937404107626
1102	37	1.0358146971609727
1102	38	0.9757418085916925
1102	39	0.9694008206529077
1102	40	1.0205109351991553
1102	41	0.9837220840410339
1102	42	0.9934333126816455
1102	43	1.0263288925953233
1102	44	1.0803008219171062
1102	45	0.959429831773555
1102	46	1.043500864872371
1102	47	1.0296745950936312
1102	48	1.0408433238499981
1102	49	1.0331000130442334
1102	50	1.1187229493400253
1103	1	0.4764878374101471
1103	2	0.622465283863864
1103	3	0.5867507761313284
1103	4	0.5656008600622684
1103	5	0.5279213744691944
1103	6	0.5739682050957029
1103	7	0.6843758749904083
1103	8	0.6308602897344215
1103	9	0.7604593215701172
1103	10	0.7535278058620847
1103	11	0.7464864338548125
1103	12	0.779065564202277
1103	13	0.7929112097839592
1103	14	0.9000280037238184
1103	15	0.9276250648603153
1103	16	0.8840892103781286
1103	17	0.866645225872702
1103	18	0.8586027841436206
1103	19	0.8057291859764604
1103	20	0.8336188208914663
1103	21	0.8940299544457986
1103	22	0.8952640001001697
1103	23	0.9592171259940443
1103	24	0.8601912407089571
1103	25	0.9461106678035449
1103	26	0.8274531169968924
1103	27	0.9196890763535279
1103	28	0.9796157336301548
1103	29	0.9558570640101536
1103	30	0.9601999920103736
1103	31	0.9429199169151445
1103	32	0.9593968587366746
1103	33	0.9604670730716478
1103	34	0.8747553476390522
1103	35	0.9201477553476636
1103	36	0.9469011622464407
1103	37	0.9332858136866591
1103	38	0.8541115141489968
1103	39	0.8847793402592856
1103	40	0.9786495001141944
1103	41	0.8880432409376269
1103	42	0.9405545480418951
1103	43	0.9962749865779372
1103	44	0.9801457489051089
1103	45	0.91871541777153
1103	46	1.0075203631711815
1103	47	1.0038953624798737
1103	48	0.977710508609924
1103	49	1.052724735825393
1103	50	1.1091550584071612
1104	1	0.6600723372460067
1104	2	0.7389651410598077
1104	3	0.6444300057297028
1104	4	0.6642860444362881
1104	5	0.7255396581729343
1104	6	0.7467000491397684
1104	7	0.7953236849968448
1104	8	0.6809490028152894
1104	9	0.7459259255441566
1104	10	0.7876951030378976
1104	11	0.779739300159342
1104	12	0.8007002415208717
1104	13	0.7979998509374866
1104	14	0.9385909504107914
1104	15	0.8363049492399455
1104	16	0.982241194950447
1104	17	0.9937814396077798
1104	18	0.9656618303482887
1104	19	0.8330231387943812
1104	20	0.945965803555687
1104	21	0.9615343294869163
1104	22	0.8913529953948256
1104	23	0.9745574659662921
1104	24	0.9659126646634135
1104	25	0.8764854868335519
1104	26	0.8258419768903522
1104	27	0.9826590225244018
1104	28	0.9986541087252618
1104	29	0.996531765095483
1104	30	1.0376449841622062
1104	31	1.0717993261217487
1104	32	1.0095824878479505
1104	33	1.0549700947473184
1104	34	1.0348702632844464
1104	35	1.0859959104275347
1104	36	1.0933379221929016
1104	37	1.0822854821743562
1104	38	1.049976742678325
1104	39	1.05827112494471
1104	40	1.0061946005772953
1104	41	1.0109931218049368
1104	42	1.0137022937501357
1104	43	1.0406991225487374
1104	44	1.0181886205096333
1104	45	1.006616123774748
1104	46	0.9671987041032182
1104	47	1.0277095836950778
1104	48	1.1670445083083152
1104	49	1.128061109287664
1104	50	1.081867215372334
1105	1	0.4640144861631974
1105	2	0.6065754307580882
1105	3	0.468016943684038
1105	4	0.48220523386315506
1105	5	0.51707836791158
1105	6	0.5833604207684431
1105	7	0.5142339474904389
1105	8	0.4770470282611612
1105	9	0.5207169872228761
1105	10	0.5743950163625016
1105	11	0.514741134894031
1105	12	0.5734655030338303
1105	13	0.6561372156258045
1105	14	0.6564307401122349
1105	15	0.7092394492114735
1105	16	0.6775210236241875
1105	17	0.6308696523863944
1105	18	0.6521991482192391
1105	19	0.6615058132048972
1105	20	0.6500695989258247
1105	21	0.7628774818141792
1105	22	0.7999286402408003
1105	23	0.7355611291090085
1105	24	0.6390317452715308
1105	25	0.6107009163196366
1105	26	0.5372962571998485
1105	27	0.5835653181950562
1105	28	0.6919094025675108
1105	29	0.6780877782802648
1105	30	0.7115454086818573
1105	31	0.7151276930180757
1105	32	0.7123371205530447
1105	33	0.762488394644194
1105	34	0.6687491138547941
1105	35	0.7793903910394491
1105	36	0.8620177356826346
1105	37	0.8447102738686993
1105	38	0.7467858859837548
1105	39	0.7270047151409003
1105	40	0.8095195827044463
1105	41	0.7494695644441691
1105	42	0.8488325599131983
1105	43	0.8787932760717467
1105	44	0.8833678867422918
1105	45	0.8444755577907301
1105	46	0.8227266331938878
1105	47	0.8294848476754382
1105	48	0.7923325300214543
1105	49	0.8260187016096125
1105	50	0.8457401821383286
1106	1	0.3911310680889777
1106	2	0.5048652170408303
1106	3	0.4027419926885507
1106	4	0.41120207454278557
1106	5	0.45291587570348213
1106	6	0.4265277646111514
1106	7	0.44329850577388896
1106	8	0.5033511241122355
1106	9	0.48243501162124147
1106	10	0.46287657739721055
1106	11	0.5052601070885103
1106	12	0.4602946168469728
1106	13	0.4637029367494421
1106	14	0.46611654795446356
1106	15	0.522938933255908
1106	16	0.5964818901091036
1106	17	0.5667925269173111
1106	18	0.4856472009313251
1106	19	0.5267157377767475
1106	20	0.5521020032387227
1106	21	0.53316510013223
1106	22	0.5677957610284778
1106	23	0.6126074900075338
1106	24	0.5606476174767749
1106	25	0.6101170553898143
1106	26	0.6127853009672037
1106	27	0.5362660113930033
1106	28	0.5553005274307594
1106	29	0.5576357476283462
1106	30	0.6755084406280432
1106	31	0.7074994488942323
1106	32	0.623750728662138
1106	33	0.7487807744736088
1106	34	0.6870142789680855
1106	35	0.6200962437930926
1106	36	0.6481390065205019
1106	37	0.6747650612905005
1106	38	0.6463118627569955
1106	39	0.6907208469511517
1106	40	0.8237626851470381
1106	41	0.7988980954603377
1106	42	0.7691670548350089
1106	43	0.739427943980111
1106	44	0.7550061020016585
1106	45	0.683794075864192
1106	46	0.6257727988253876
1106	47	0.5803784446693285
1106	48	0.6295380335475258
1106	49	0.7121482963303976
1106	50	0.8658814127712233
1107	1	0.34050512914050657
1107	2	0.48882890289004166
1107	3	0.3818798769500269
1107	4	0.3984005212463728
1107	5	0.39197614443490636
1107	6	0.430114245096334
1107	7	0.4416148910289124
1107	8	0.37243400431555074
1107	9	0.49389927863808936
1107	10	0.4805482630642999
1107	11	0.4779308725606768
1107	12	0.552135861258563
1107	13	0.5936507866202092
1107	14	0.46780539983154057
1107	15	0.5079927127493562
1107	16	0.543345855356236
1107	17	0.5117427516734594
1107	18	0.6250376307932797
1107	19	0.6723002161987495
1107	20	0.6376797916865584
1107	21	0.743327945982422
1107	22	0.6129073079374407
1107	23	0.7022063792919155
1107	24	0.6934171417055026
1107	25	0.6452254951391012
1107	26	0.6735474626507809
1107	27	0.690660054568049
1107	28	0.639374365571463
1107	29	0.8112239994964483
1107	30	0.7945609300618202
1107	31	0.7638828444251899
1107	32	0.7004957857490339
1107	33	0.7349940242083477
1107	34	0.6297011384552746
1107	35	0.673210652271031
1107	36	0.7093163495533332
1107	37	0.7337300240757472
1107	38	0.6774931003677125
1107	39	0.615688393675768
1107	40	0.6104005677141444
1107	41	0.6192113843996268
1107	42	0.6054470656814175
1107	43	0.5980266402299605
1107	44	0.652105143624965
1107	45	0.6777697719976601
1107	46	0.7716323499095097
1107	47	0.7260241667302907
1107	48	0.8327474774891712
1107	49	0.8159828197147121
1107	50	0.7717018812949163
1108	1	0.337146007168716
1108	2	0.482207661721084
1108	3	0.31318158005672814
1108	4	0.38807344731510085
1108	5	0.35732355218703304
1108	6	0.3703306726281639
1108	7	0.42240555804280433
1108	8	0.377543260876422
1108	9	0.4873901080484231
1108	10	0.423219714031255
1108	11	0.43040565509186024
1108	12	0.4455258180705428
1108	13	0.4182149753319351
1108	14	0.44073339590641325
1108	15	0.423990337119335
1108	16	0.44478746605493313
1108	17	0.36961341490285093
1108	18	0.4120752099283749
1108	19	0.41771637368405123
1108	20	0.38290633083482106
1108	21	0.3984229320009831
1108	22	0.3525229355019732
1108	23	0.41311799024870055
1108	24	0.39988847032067554
1108	25	0.3251044782777157
1108	26	0.3739030477126093
1108	27	0.42336073979915767
1108	28	0.45645772208894186
1108	29	0.4831328098687635
1108	30	0.5807940155055604
1108	31	0.5340631809340479
1108	32	0.5134822727421096
1108	33	0.5272783763143849
1108	34	0.4750831331730154
1108	35	0.34595757145716277
1108	36	0.481209453705965
1108	37	0.5258752864266469
1108	38	0.5220644290403698
1108	39	0.5538352068002865
1108	40	0.5754067713834843
1108	41	0.5250072880807792
1108	42	0.4360784965591762
1108	43	0.44538212402282795
1108	44	0.49982304140237943
1108	45	0.4481507402590239
1108	46	0.49421806022685644
1108	47	0.5024293824781125
1108	48	0.4749879922683107
1108	49	0.4746814920928628
1108	50	0.5090367636469821
1109	1	0.18467212639530572
1109	2	0.30158602300272364
1109	3	0.16304549669131418
1109	4	0.15529862982889142
1109	5	0.1539246422046645
1109	6	0.04386746691378818
1109	7	0.059200789159405076
1109	8	0.06568016817089983
1109	9	0.054075189304484184
1109	10	0.11235992379549486
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2211	1	-0.17548890469349132
2211	2	-0.2059916370315804
2211	3	-0.18785955604906235
2211	4	-0.15670617515452534
2211	5	-0.2322504466678481
2211	6	-0.2198150368091234
2211	7	-0.3098243867086694
2211	8	-0.3326097184032122
2211	9	-0.47615373987376586
2211	10	-0.44074725751172394
2211	11	-0.41130269248097306
2211	12	-0.32939723026296264
2211	13	-0.2788392412364544
2211	14	-0.3714666242266276
2211	15	-0.46808927938743494
2211	16	-0.4210526252576514
2211	17	-0.3900431979991481
2211	18	-0.47852291497883215
2211	19	-0.42828817986173107
2211	20	-0.31018811573588323
2211	21	-0.3768479059639276
2211	22	-0.49743560905970696
2211	23	-0.3180735181738328
2211	24	-0.32736518737348064
2211	25	-0.3523473736831022
2211	26	-0.3702991961025788
2211	27	-0.355982675055472
2211	28	-0.35301359710170743
2211	29	-0.2921991160198232
2211	30	-0.3602061995717989
2211	31	-0.36090341640527157
2211	32	-0.3755902593459055
2211	33	-0.3011039311168844
2211	34	-0.34377153042589015
2211	35	-0.36027072033039786
2211	36	-0.4013471596659386
2211	37	-0.2121107576825665
2211	38	-0.23911862309734033
2211	39	-0.35402248933390723
2211	40	-0.25798903624541936
2211	41	-0.23054160705233073
2211	42	-0.40473279543114415
2211	43	-0.3620563069824527
2211	44	-0.40454683491369536
2211	45	-0.4947809973281565
2211	46	-0.4945515102001252
2211	47	-0.4544053886396284
2211	48	-0.4287529381962045
2211	49	-0.4760829548289536
2211	50	-0.5230285589277557
2212	1	-0.9423044862037138
2212	2	-2.4117627582298766
2212	3	-1.3041402912413602
2212	4	-0.6943412185661444
2212	5	-0.6662019946439628
2212	6	-0.6480497790491029
2212	7	-0.5660572446463448
2212	8	-0.6354275899078132
2212	9	-0.8145608150784085
2212	10	-0.8085469217794028
2212	11	-0.73154576072411
2212	12	-0.722141600936034
2212	13	-2.1253149193908243
2212	14	-1.644918543310851
2212	15	-1.1903066206510928
2212	16	-1.003430096119203
2212	17	-0.7425662382583766
2212	18	-0.8701729126382958
2212	19	-0.7218179559602262
2212	20	-0.782264646682016
2212	21	-0.7170223151311745
2212	22	-0.74756407627725
2212	23	-0.7419123119315785
2212	24	-0.7592547972080865
2212	25	-0.7059709002837593
2212	26	-0.8410431089165087
2212	27	-0.9364480716404415
2212	28	-0.8705821368221507
2212	29	-0.9137790002136015
2212	30	-0.8234896108879042
2212	31	-0.7277779012846253
2212	32	-0.787157449942228
2212	33	-0.7931963997997613
2212	34	-0.7213946429504787
2212	35	-0.8463725353017395
2212	36	-0.7760189612950938
2212	37	-0.8008968301011663
2212	38	-0.8229898710569401
2212	39	-0.7414636936757901
2212	40	-0.6486474812430529
2212	41	-0.7006385842271428
2212	42	-0.7433807357520266
2212	43	-0.7436847263413711
2212	44	-0.9293840923048222
2212	45	-0.8994776807837681
2212	46	-0.7692872863493889
2212	47	-0.8699972158306366
2212	48	-0.7953010004808831
2212	49	-0.7663270210000803
2212	50	-0.8340308663887868
2213	1	-0.8666287445714396
2213	2	-2.123705694031237
2213	3	-1.242463393781776
2213	4	-0.8012842463243857
2213	5	-0.8085471262814252
2213	6	-0.678098872236319
2213	7	-0.7067918339692554
2213	8	-0.7898310986052834
2213	9	-0.8042107729432928
2213	10	-0.7867155909840804
2213	11	-0.7007287686008041
2213	12	-0.7099744671755488
2213	13	-0.7924266927486983
2213	14	-0.8771419216747631
2213	15	-0.9692257051943685
2213	16	-0.9412855743209434
2213	17	-0.9752686176781467
2213	18	-1.0087310711238686
2213	19	-0.9886872696647728
2213	20	-0.9777010858473503
2213	21	-0.9659403480649402
2213	22	-1.0325733002601751
2213	23	-1.0186292648671014
2213	24	-0.8979750591767512
2213	25	-0.9590730304296358
2213	26	-0.9815022402116043
2213	27	-1.0047107915176028
2213	28	-1.0238702556625157
2213	29	-1.0204537024972922
2213	30	-1.0536589907542506
2213	31	-1.0954107990252666
2213	32	-0.9188772582569271
2213	33	-0.919530717779125
2213	34	-0.9371621599118072
2213	35	-0.8722311060842571
2213	36	-0.9479243999082101
2213	37	-1.0030012044666066
2213	38	-0.9953696539349508
2213	39	-0.9983622873956506
2213	40	-1.0673161553992543
2213	41	-1.084541130678339
2213	42	-1.1335230873677986
2213	43	-1.1155518260070707
2213	44	-0.9978335553945072
2213	45	-1.0953681865419185
2213	46	-1.0087044046658349
2213	47	-0.9981183857674338
2213	48	-1.1394579539068488
2213	49	-1.0834653911185228
2213	50	-1.189251765689395
2214	1	-0.9939432464769951
2214	2	-1.9342136433259114
2214	3	-1.118743243535711
2214	4	-0.8326673923940575
2214	5	-0.7920450220521573
2214	6	-0.7364829970638809
2214	7	-0.8025707222846492
2214	8	-0.7988278846494978
2214	9	-0.8498396699756157
2214	10	-0.8590093282790505
2214	11	-0.8462718704474455
2214	12	-0.7924213790784691
2214	13	-0.8219921946677076
2214	14	-0.9807769523328744
2214	15	-0.992829257578812
2214	16	-0.9971404444787879
2214	17	-0.9498331362589454
2214	18	-0.9692324218899131
2214	19	-0.9522962840850582
2214	20	-0.9837648330730318
2214	21	-1.05177317827836
2214	22	-0.9615997553502424
2214	23	-0.9615287470901492
2214	24	-0.9550082175703523
2214	25	-0.989383493348619
2214	26	-0.9360352003630794
2214	27	-0.9066583450952237
2214	28	-0.9434787925840737
2214	29	-0.9628705617522471
2214	30	-0.9040873512016674
2214	31	-0.8693056456746007
2214	32	-0.9016918556336009
2214	33	-0.8446046971613725
2214	34	-0.9121041830349981
2214	35	-0.9644744998978965
2214	36	-0.9771001162922843
2214	37	-0.9761934008548465
2214	38	-1.0331406910032186
2214	39	-1.0526315640451926
2214	40	-0.9409877685290979
2214	41	-1.065569201477544
2214	42	-0.9751563105157288
2214	43	-0.9565283738916364
2214	44	-0.9458566161785527
2214	45	-1.1861173643022767
2214	46	-0.9696988272630875
2214	47	-0.9729411487159325
2214	48	-0.9774988292868301
2214	49	-1.0197460767815087
2214	50	-1.1691092202948106
2215	1	-0.6761116577809112
2215	2	-0.5109846422368456
2215	3	-0.7189126984436882
2215	4	-0.6850003141595593
2215	5	-0.8021643606308686
2215	6	-0.7672912832962311
2215	7	-0.887707571606164
2215	8	-0.9345103329108326
2215	9	-1.0890800678325618
2215	10	-1.1050750737257573
2215	11	-1.007870218060937
2215	12	-0.9359240989262929
2215	13	-0.9331797991940718
2215	14	-1.1126086866668459
2215	15	-1.1733695419428187
2215	16	-1.1874253516064712
2215	17	-1.2650112992663551
2215	18	-1.1906334663790688
2215	19	-1.207165155677103
2215	20	-1.2240007181671544
2215	21	-1.1988807173722762
2215	22	-1.2983998335602038
2215	23	-1.2225000874052512
2215	24	-1.1713775938660547
2215	25	-1.2071542118462801
2215	26	-1.2428280007019836
2215	27	-1.2395686043990466
2215	28	-1.3707315833398468
2215	29	-1.4176503608926652
2215	30	-1.3196929734676885
2215	31	-1.2741197421343529
2215	32	-1.270725540227808
2215	33	-1.3087120797549048
2215	34	-1.3290728471534052
2215	35	-1.312203212728555
2215	36	-1.2657862654455982
2215	37	-1.2117125496215309
2215	38	-1.2009583394985412
2215	39	-1.2454125113914642
2215	40	-1.2442222571892572
2215	41	-1.3807348927324767
2215	42	-1.3439477212600224
2215	43	-1.3085318402341954
2215	44	-1.2427607651282884
2215	45	-1.2165346812099074
2215	46	-1.2485018769686533
2215	47	-1.281564641394013
2215	48	-1.2644993607444164
2215	49	-1.1326621052766441
2215	50	-1.206047734957366
2216	1	-1.206327731780842
2216	2	-0.7711118043908947
2216	3	-0.9022630377279839
2216	4	-2.2311153202356024
2216	5	-1.570341554417226
2216	6	-1.4074991072296967
2216	7	-1.3123144974260796
2216	8	-1.2881873683487441
2216	9	-1.3311573005145902
2216	10	-1.3586835620997917
2216	11	-1.572298453072463
2216	12	-1.47478966121529
2216	13	-1.5112702040752382
2216	14	-1.7311161447418826
2216	15	-1.6799098188869053
2216	16	-1.6989289492683497
2216	17	-1.63506677230464
2216	18	-1.6855673624411835
2216	19	-1.7298828636187693
2216	20	-1.7647449085480886
2216	21	-1.7404572898745627
2216	22	-1.810763595959287
2216	23	-1.756114112368742
2216	24	-1.8549910581937965
2216	25	-1.8353046047522739
2216	26	-2.054836768546707
2216	27	-2.0790313558846734
2216	28	-2.006638135078674
2216	29	-2.0129150320449156
2216	30	-1.848881838195254
2216	31	-1.800344862054338
2216	32	-1.8056544927285074
2216	33	-1.7835207223162328
2216	34	-1.7707827014603694
2216	35	-1.8617214023684525
2216	36	-1.920679955203724
2216	37	-1.864088312117289
2216	38	-1.9208372870282702
2216	39	-1.9590266410069646
2216	40	-1.96895100292749
2216	41	-2.068544453988906
2216	42	-2.067561649046649
2216	43	-1.9498510186362168
2216	44	-1.8484175548881439
2216	45	-1.7379820383854607
2216	46	-1.7558772769199642
2216	47	-1.7925264157538194
2216	48	-1.7926493798932412
2216	49	-1.8535209693389234
2216	50	-1.8785694072665216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106831.0
3	17579.0
4	29308.0
5	17196.0
6	12755.0
7	9832.0
8	8094.0
9	7198.0
10	6929.0
11	6948.0
12	7134.0
13	7348.0
14	7995.0
15	8674.0
16	9234.0
17	9991.0
18	10794.0
19	11673.0
20	13697.0
21	16984.0
22	21048.0
23	25240.0
24	29375.0
25	33520.0
26	36477.0
27	41006.0
28	48087.0
29	55404.0
30	63822.0
31	76800.0
32	92932.0
33	114654.0
34	145622.0
35	186424.0
36	243702.0
37	329986.0
38	521015.0
39	1347849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48634486654609	17.874099193440102	13.87853294580797	26.76102299420584
2	14.864854022660815	19.743941257111924	37.968656980665614	27.422547739561644
3	17.605197986702827	24.043630353503747	29.345437720973273	29.005733938820157
4	12.299268589352023	16.309831626851125	36.31603101942833	35.074868764368524
5	14.08570117624082	37.18060707563765	34.16491985162216	14.568771896499369
6	32.97173405011171	36.38839786336126	16.982522106849057	13.657345979677963
7	28.619512496565775	31.51100276155819	21.482290367825215	18.38719437405082
8	26.923137624784964	34.065522822885406	19.667332828473455	19.34400672385618
9	26.763116838090834	14.984660640069169	18.673436958280366	39.578785563559634
10	14.594743537097402	28.39546663848809	33.395861227667254	23.613928596747247
11	35.3591387849876	22.371407267772472	23.31907980894588	18.950374138294055
12	23.68433514269272	24.220936077952008	30.054280781649677	22.040447997705595
13	28.376718593786244	21.20005875008394	25.76105570369411	24.662166952435708
14	22.551961571740225	20.39398383911243	26.753915411382394	30.300139177764944
15	23.955469611958954	28.203363857102957	23.46548231444156	24.37568421649653
16	24.042926506644292	27.260996499399088	24.884740226731065	23.81133676722556
17	22.86387913352772	27.111425518241113	26.336237734599123	23.688457613632046
18	22.966494375059867	26.55695228473226	27.159232655743388	23.317320684464484
19	23.75832166963978	26.34071827303665	27.10531786831474	22.795642189008827
20	24.006673946005357	25.700016508277283	27.09278534771328	23.20052419800408
21	24.174700132976945	25.577593518644864	27.054788106456474	23.192918241921717
22	23.925844767917077	25.25909248401031	27.0562786199036	23.758784128169008
23	23.63670318391992	25.392485112080127	26.99568224906183	23.975129454938124
24	23.512529670748332	25.554166104822016	26.88070807408216	24.052596150347487
25	23.62496933317959	25.441916573941615	27.025408045957022	23.907706046921774
26	23.42501135741587	25.913609796112315	27.03075679184171	23.630622054630106
27	23.570088537057035	25.802992473333468	26.9230563424446	23.703862647164897
28	23.561814845751165	25.708557916686956	27.002174086367358	23.727453151194517
29	23.7392481532909	25.775350279447686	26.795461416421197	23.689940150840215
30	23.692639927102615	25.792479487578042	26.88535168680878	23.629528898510564
31	23.84048840127237	25.433892201791906	26.734137526050656	23.991481870885067
32	23.58950625127908	25.84949044083322	26.74006530168926	23.820938006198446
33	23.771029432377862	25.62728511004373	26.66167691901094	23.94000853856747
34	23.917090174118876	25.651029859381886	26.74411108641264	23.687768880086605
35	23.85888314634934	25.600200300825733	26.815380043264764	23.725536509560165
36	23.898801586471496	25.802364085418226	26.505934823274064	23.792899504836214
37	23.908371757855697	25.468998512236734	26.723727015501947	23.898902714405626
38	23.887843597547437	25.596328753726805	26.684317437831318	23.83151021089444
39	23.912820194593575	25.832302662127432	26.521318845448533	23.733558297830452
40	23.84955244510977	25.578817776810165	26.80555172938569	23.76607804869438
41	23.63599259780507	25.693126651726406	26.6925139031868	23.97836684728172
42	24.064859648709888	25.68223201237582	26.56158061292828	23.69132772598601
43	23.820593555123303	25.544581623791846	26.61648118793398	24.01834363315087
44	23.907239815373984	25.76697945824278	26.28401360999064	24.04176711639259
45	23.853165862368137	25.76069595803883	26.28791177285511	24.098226406737922
46	24.002300003343027	25.586093003042155	26.16018453515194	24.251422458462876
47	23.8253524938736	25.83971781769245	26.3098522843336	24.02507740410035
48	23.90553012337867	25.956028914677955	26.123901785945908	24.014539175997466
49	23.998542985147434	25.71256798332884	26.130176039914932	24.158712991608795
50	24.123089003163287	25.76546849710148	26.003570866946156	24.107871632789074
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	750.0
1	543.5
2	337.0
3	480.5
4	624.0
5	771.0
6	918.0
7	858.5
8	799.0
9	1046.5
10	1294.0
11	1887.0
12	2480.0
13	4711.5
14	6943.0
15	9454.0
16	11965.0
17	14015.5
18	16066.0
19	17303.0
20	18540.0
21	20244.5
22	21949.0
23	25738.0
24	29527.0
25	35441.0
26	41355.0
27	48908.0
28	56461.0
29	64299.5
30	72138.0
31	80350.0
32	88562.0
33	99854.5
34	111147.0
35	123144.0
36	135141.0
37	145937.0
38	156733.0
39	167837.0
40	178941.0
41	191674.0
42	204407.0
43	213657.5
44	222908.0
45	233504.0
46	244100.0
47	254694.0
48	265288.0
49	268823.0
50	272358.0
51	273766.0
52	275174.0
53	269878.0
54	264582.0
55	255470.5
56	246359.0
57	231017.5
58	215676.0
59	196549.0
60	177422.0
61	156178.5
62	134935.0
63	115791.0
64	96647.0
65	80567.0
66	64487.0
67	52216.5
68	39946.0
69	32536.5
70	25127.0
71	20009.5
72	14892.0
73	12249.0
74	9606.0
75	7496.0
76	5386.0
77	4275.0
78	3164.0
79	2498.0
80	1832.0
81	1441.5
82	1051.0
83	814.0
84	577.0
85	445.0
86	313.0
87	223.0
88	133.0
89	96.0
90	59.0
91	45.5
92	32.0
93	23.5
94	15.0
95	12.0
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015056869770378725
2	4.01159940596236E-4
3	4.5464793267573414E-4
4	0.0021662636792196743
5	0.03749508244772819
6	0.025380052241721865
7	0.028081195841736517
8	0.02209054072883273
9	0.02837537979817376
10	0.02920444367540598
11	0.03658578658237672
12	0.03663927457445622
13	0.034499754891276296
14	0.039875298095265854
15	0.034900914831872534
16	0.04046366600814034
17	0.043084577620035744
18	0.045331073287374665
19	0.04749733696659434
20	0.043806665513108974
21	0.04458224139826169
22	0.04257644169528051
23	0.04180086581012779
24	0.03928693018239138
25	0.03754857043980769
26	0.04041017801606084
27	0.04046366600814034
28	0.039982274079424854
29	0.03816368234872192
30	0.03476719485167379
31	0.03334976306156709
32	0.030006763556598454
33	0.02206379673279298
34	0.019630093093175816
35	0.020860316911004272
36	0.021341708839719756
37	0.017624293390194636
38	0.018694053231784597
39	0.01318479004759629
40	0.007648782867368233
41	0.007113902946573252
42	0.006766230998056513
43	0.002513935627736412
44	0.00371741544952512
45	0.0021662636792196743
46	8.558078732719701E-4
47	3.744159445564869E-4
48	4.8139192871548317E-4
49	1.3371998019874533E-4
50	2.4069596435774158E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3739157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.63045446212284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24294662501524	36.78305711470297
2	14.035871900122403	14.493568899509187
3	5.846844396945501	9.056257001508383
4	2.940094503638587	6.071936615377989
5	1.6573628317704456	4.278519810646948
6	1.0610930340370222	3.287082934435455
7	0.7022997807918896	2.5382039795654157
8	0.4806334863548366	1.9852260264171786
9	0.3455793370002049	1.6058176399835713
>10	1.5748623654473584	13.742728863662684
>50	0.07808789590523008	2.7488710113604746
>100	0.033332454796688664	2.852717380043894
>500	7.826748775529313E-4	0.3024812678384889
>1k	2.0871329711250272E-4	0.25353145494736534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3817	0.10208183288372219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6743996039749067E-5
2	0.0	0.0	0.0	0.0	2.6743996039749067E-5
3	0.0	0.0	0.0	0.0	2.6743996039749067E-5
4	0.0	0.0	0.0	0.0	2.6743996039749067E-5
5	0.0	0.0	0.0	0.0	2.6743996039749067E-5
6	0.0	0.0	0.0	0.0	8.02319881192472E-5
7	0.0	0.0	0.0	0.0	8.02319881192472E-5
8	0.0	0.0	0.0	0.0	8.02319881192472E-5
9	0.0	0.0	0.0	0.0	1.0697598415899627E-4
10	0.0	0.0	0.0	0.0	1.0697598415899627E-4
11	0.0	0.0	0.0	0.0	1.0697598415899627E-4
12	0.0	0.0	0.0	0.0	1.0697598415899627E-4
13	0.0	0.0	0.0	0.0	1.0697598415899627E-4
14	0.0	0.0	0.0	0.0	1.604639762384944E-4
15	0.0	0.0	0.0	0.0	2.1395196831799253E-4
16	0.0	0.0	0.0	0.0	2.406959643577416E-4
17	0.0	0.0	0.0	0.0	2.406959643577416E-4
18	0.0	0.0	0.0	0.0	2.406959643577416E-4
19	0.0	0.0	0.0	2.6743996039749067E-5	2.406959643577416E-4
20	0.0	0.0	0.0	2.6743996039749067E-5	2.406959643577416E-4
21	0.0	0.0	0.0	2.6743996039749067E-5	2.406959643577416E-4
22	0.0	0.0	0.0	2.6743996039749067E-5	2.406959643577416E-4
23	0.0	0.0	0.0	8.02319881192472E-5	3.209279524769888E-4
24	0.0	0.0	0.0	2.1395196831799253E-4	3.476719485167379E-4
25	0.0	0.0	0.0	2.1395196831799253E-4	3.476719485167379E-4
26	0.0	0.0	0.0	2.406959643577416E-4	3.476719485167379E-4
27	0.0	0.0	0.0	2.9418395643723974E-4	3.476719485167379E-4
28	0.0	0.0	0.0	6.418559049539776E-4	3.476719485167379E-4
29	0.0	0.0	0.0	0.0010965038376297117	3.476719485167379E-4
30	0.0	2.6743996039749067E-5	0.0	0.0017383597425836893	3.476719485167379E-4
31	0.0	2.6743996039749067E-5	0.0	0.0038778794257636148	3.476719485167379E-4
32	0.0	2.6743996039749067E-5	0.0	0.005990655112903791	3.476719485167379E-4
33	0.0	2.6743996039749067E-5	0.0	0.008558078732719701	3.476719485167379E-4
34	0.0	2.6743996039749067E-5	0.0	0.01235572617036407	3.476719485167379E-4
35	0.0	2.6743996039749067E-5	0.0	0.01596616563573019	3.476719485167379E-4
36	0.0	2.6743996039749067E-5	0.0	0.020539388958527284	3.476719485167379E-4
37	0.0	2.6743996039749067E-5	0.0	0.02695794800806706	3.476719485167379E-4
38	0.0	2.6743996039749067E-5	0.0	0.033617203021964574	3.476719485167379E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2570	0.0	26.541773	1
GTATCAA	5905	0.0	24.631052	1
TCGATCG	80	9.017601E-5	19.243706	41
CGAATCG	360	0.0	17.107803	37
TATCAAC	8460	0.0	16.983257	2
ATCAACG	8560	0.0	16.70774	3
TCAACGC	8580	0.0	16.66924	4
CAACGCA	8690	0.0	16.383373	5
AACGCAG	8940	0.0	15.947066	6
TACCGAT	445	0.0	15.820974	7
GTACCGA	505	0.0	14.812585	6
GCCGTAC	285	5.456968E-12	13.897255	1
ACCGATC	700	0.0	13.8294115	8
CGATAAC	145	3.836369E-5	13.65655	10
TGCGATA	275	4.3655746E-11	13.602101	13
GTATACG	130	2.3289764E-4	13.5409155	1
GTACATG	8750	0.0	13.479014	1
GAGTACT	6615	0.0	13.404584	12
ACGCAGA	10600	0.0	13.387428	7
CCAGTAC	1090	0.0	13.322803	3
>>END_MODULE