FastQCFastQC Report
Mon 6 Feb 2017
SRR2077449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921200
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33060.11317266876626043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58800.020.018061
GGTATCA30800.016.3580041
TATCAAC71350.016.3422762
TCAACGC71300.016.2920254
ATCAACG71550.016.265013
CAACGCA71800.016.117015
GAGTACT34800.015.86973812
AACGCAG73800.015.6772796
GTACATG80400.014.7768961
AGAGTAC71550.014.39143311
AGTACTT38150.014.36084813
TACATGG80800.014.3220442
GTACTTT39250.013.90160714
ACGCAGA85300.013.4356847
ACATGGG84000.013.382893
CGCAGAG87150.013.1507018
CATGGGG55200.012.7140014
CAGAGTA86700.012.46056310
GTCCTAG6400.012.375651
TCGATCG1250.00267328712.31600641
Produced by FastQC (version 0.11.5)