##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077449_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2921200
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.13062337395591	31.0	30.0	34.0	23.0	34.0
2	29.115378611529508	31.0	30.0	34.0	22.0	34.0
3	29.240035259482404	31.0	30.0	34.0	22.0	34.0
4	32.60817027249076	37.0	33.0	37.0	23.0	37.0
5	32.67127653019307	37.0	33.0	37.0	25.0	37.0
6	32.69581370669588	37.0	35.0	37.0	25.0	37.0
7	32.543455086950566	37.0	33.0	37.0	23.0	37.0
8	32.444087703683415	37.0	33.0	37.0	22.0	37.0
9	33.65942078597836	38.0	34.0	39.0	18.0	39.0
10	33.5896943037108	38.0	34.0	39.0	18.0	39.0
11	33.62170991373408	38.0	34.0	39.0	18.0	39.0
12	33.55600712036149	38.0	34.0	39.0	18.0	39.0
13	33.563960358756674	38.0	34.0	39.0	18.0	39.0
14	34.4315842804327	39.0	34.0	41.0	17.0	41.0
15	34.53296419279748	39.0	34.0	41.0	17.0	41.0
16	34.43413528686841	39.0	34.0	41.0	17.0	41.0
17	34.26500890045187	39.0	33.0	41.0	17.0	41.0
18	34.08250308092565	39.0	33.0	41.0	17.0	41.0
19	34.42338593728605	39.0	34.0	41.0	18.0	41.0
20	34.46371901958099	39.0	34.0	41.0	18.0	41.0
21	34.47417602355196	39.0	34.0	41.0	18.0	41.0
22	34.37763179515267	39.0	34.0	41.0	18.0	41.0
23	34.120795221142	39.0	33.0	41.0	17.0	41.0
24	34.272948788169245	39.0	34.0	41.0	18.0	41.0
25	34.172348007668084	39.0	34.0	41.0	17.0	41.0
26	34.21312508558127	39.0	34.0	41.0	17.0	41.0
27	34.198417088867586	39.0	34.0	41.0	17.0	41.0
28	34.083905586745175	39.0	33.0	41.0	16.0	41.0
29	34.04992400383404	39.0	33.0	41.0	16.0	41.0
30	33.8334400246474	39.0	33.0	41.0	13.0	41.0
31	33.744620019170206	39.0	33.0	41.0	10.0	41.0
32	33.76645419690538	39.0	33.0	41.0	14.0	41.0
33	33.75019717924141	39.0	33.0	41.0	10.0	41.0
34	33.73103484869232	39.0	33.0	41.0	9.0	41.0
35	33.66577057373682	39.0	33.0	41.0	9.0	41.0
36	33.541153635492265	38.0	33.0	41.0	9.0	41.0
37	33.43753491715733	38.0	33.0	41.0	8.0	41.0
38	33.33375701766398	38.0	33.0	41.0	8.0	41.0
39	33.26970936601397	38.0	33.0	40.0	7.0	41.0
40	33.23620498425305	38.0	33.0	40.0	2.0	41.0
41	33.114496439819256	38.0	33.0	40.0	2.0	41.0
42	32.933965493632755	38.0	32.0	40.0	2.0	41.0
43	32.82688758044639	38.0	32.0	40.0	2.0	41.0
44	32.74739319457757	38.0	32.0	40.0	2.0	41.0
45	32.65726961522662	38.0	32.0	40.0	2.0	41.0
46	32.50064836368615	38.0	32.0	40.0	2.0	41.0
47	32.31212310009585	38.0	32.0	40.0	2.0	41.0
48	32.11411132411338	38.0	31.0	40.0	2.0	41.0
49	31.978552649596057	38.0	31.0	40.0	2.0	41.0
50	31.771754415993428	38.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8532080185661215
1101	2	0.9307638972804924
1101	3	0.9822072525921932
1101	4	0.8689048930063805
1101	5	0.8189685849247113
1101	6	0.9262424057163727
1101	7	1.0001061523197734
1101	8	0.9686348509761729
1101	9	1.278734774538954
1101	10	1.269292471613909
1101	11	1.2709116113822176
1101	12	1.2935928837206276
1101	13	1.2249094005583672
1101	14	1.5439265173713181
1101	15	1.4266160687654619
1101	16	1.3263122683061184
1101	17	1.4771313424535109
1101	18	1.609996093104911
1101	19	1.2654471088981083
1101	20	1.3233529175781342
1101	21	1.4301111284962005
1101	22	1.283028225094128
1101	23	1.3273722145647469
1101	24	1.1490163426802553
1101	25	1.2795582978775357
1101	26	1.2062267570697216
1101	27	1.228101081754069
1101	28	1.3453169885051324
1101	29	1.3135883053032416
1101	30	1.4302991414912825
1101	31	1.4504063840705612
1101	32	1.3821893657641624
1101	33	1.313428868249261
1101	34	1.2439640783258241
1101	35	1.2609726338656202
1101	36	1.388372741536294
1101	37	1.3018888644584123
1101	38	1.4135556601964794
1101	39	1.3543784239738912
1101	40	1.2558224714284592
1101	41	1.3087207188103775
1101	42	1.3800062255584393
1101	43	1.3824951153828025
1101	44	1.1318861140228194
1101	45	1.255673846741331
1101	46	1.2496012117740278
1101	47	1.2545537516271992
1101	48	1.248220693065619
1101	49	1.2937922168854783
1101	50	1.3452665969300206
1102	1	0.6009598711667756
1102	2	0.5639954458497627
1102	3	0.57399521896129
1102	4	0.5544173030938637
1102	5	0.4813558644947591
1102	6	0.5572414858656032
1102	7	0.6444207517799185
1102	8	0.5861688704642489
1102	9	0.79706832064916
1102	10	0.8118016268647992
1102	11	0.7040058084555838
1102	12	0.6262517741331664
1102	13	0.7370775615213887
1102	14	1.0535374588931177
1102	15	0.9801621862423033
1102	16	1.129796851959462
1102	17	1.1675428165462023
1102	18	1.1618570385626938
1102	19	0.9847305075791368
1102	20	0.8743499213992365
1102	21	0.8637403593719881
1102	22	1.0089951556716628
1102	23	1.140223604912201
1102	24	1.067449953673119
1102	25	1.0528579236136721
1102	26	0.918278392170329
1102	27	0.9777096268731285
1102	28	0.8863481888685669
1102	29	0.8000201296978418
1102	30	1.0319615653508123
1102	31	1.0419446298944095
1102	32	0.9668881472411783
1102	33	0.8874534733611128
1102	34	0.8156896020881703
1102	35	0.8830132622448161
1102	36	0.8967530065882059
1102	37	0.9363817979541267
1102	38	0.9964030490917537
1102	39	1.0300515536015595
1102	40	0.9861495871566817
1102	41	0.9337330008940015
1102	42	0.9341930118498709
1102	43	1.0056685792442224
1102	44	0.9287772647029797
1102	45	0.9570484476677947
1102	46	1.0451290316871678
1102	47	1.0097244763566522
1102	48	0.9108510942401651
1102	49	0.8510934953704918
1102	50	0.9237031914754574
1103	1	0.35063358323718674
1103	2	0.33331615170748563
1103	3	0.456741634348635
1103	4	0.4655651063480022
1103	5	0.3496737556276912
1103	6	0.40248164635137584
1103	7	0.5091834314125734
1103	8	0.4877378259800338
1103	9	0.6501250916192305
1103	10	0.6543574403317649
1103	11	0.6801379233759093
1103	12	0.630726907168615
1103	13	0.5602151784141967
1103	14	0.8250096015347452
1103	15	0.6459209581549388
1103	16	0.800295023795428
1103	17	0.9507555741892446
1103	18	0.744306144795587
1103	19	0.6230291865652973
1103	20	0.7238378934900425
1103	21	0.7000024174711115
1103	22	0.7233134898447489
1103	23	0.9376861135423553
1103	24	0.7159795219216818
1103	25	0.5283617876454301
1103	26	0.6324467053949334
1103	27	0.6129069229948882
1103	28	0.6496719243555802
1103	29	0.72089461340925
1103	30	0.8482845773480605
1103	31	0.8576292895247093
1103	32	0.690498263965921
1103	33	0.6657954864173163
1103	34	0.6024413376213857
1103	35	0.5642000704366765
1103	36	0.6585990764439273
1103	37	0.5689868053663787
1103	38	0.490840340513401
1103	39	0.6401935193207535
1103	40	0.5661377493435751
1103	41	0.6209649713962548
1103	42	0.5939021941038973
1103	43	0.6693860382985051
1103	44	0.5669457694740103
1103	45	0.48695057935453434
1103	46	0.4766151820514182
1103	47	0.38034873601624497
1103	48	0.3571697771050921
1103	49	0.4438130316653073
1103	50	0.5518735735439044
1104	1	0.38326668343339776
1104	2	0.41832930630346254
1104	3	0.4661248087525536
1104	4	0.5019643595192562
1104	5	0.45391127914005835
1104	6	0.4609209364942686
1104	7	0.5454883468555494
1104	8	0.543406877300356
1104	9	0.7508364318889562
1104	10	0.8198032999194496
1104	11	0.663208525970326
1104	12	0.7019398637050713
1104	13	0.5813900221107957
1104	14	0.9388281203986111
1104	15	0.7990935190574504
1104	16	0.8105329536857013
1104	17	1.0051842185829258
1104	18	1.12904273775424
1104	19	0.9239546108514674
1104	20	0.9164891037466703
1104	21	0.7856382607181942
1104	22	0.7640982544905057
1104	23	0.9437687597543984
1104	24	0.8123874116175145
1104	25	0.9528255487365769
1104	26	0.80311270192243
1104	27	0.8004247293653606
1104	28	0.8937792458977256
1104	29	1.057065368474909
1104	30	1.0886800224163622
1104	31	1.092233252981714
1104	32	0.9405629285520547
1104	33	0.9094800278136859
1104	34	0.7726500595966712
1104	35	0.8425827539662976
1104	36	0.8970210338931395
1104	37	0.8710653070804568
1104	38	0.8410523071498517
1104	39	0.877458671560376
1104	40	0.7908529020901724
1104	41	0.9539644777385519
1104	42	0.9725678212330848
1104	43	1.0112252084764108
1104	44	1.0193951804944419
1104	45	0.8770613411918333
1104	46	0.9572330037107619
1104	47	0.9432688694370341
1104	48	0.842441709334615
1104	49	0.7948130513479938
1104	50	0.9203020660429111
1105	1	0.35867802349277156
1105	2	0.5494364190358318
1105	3	0.4553250782323275
1105	4	0.4657091972288967
1105	5	0.31519355372460467
1105	6	0.3846217372654621
1105	7	0.3948946149988757
1105	8	0.390333329040466
1105	9	0.6019940446925318
1105	10	0.5492554672598189
1105	11	0.4699909813035035
1105	12	0.5120812310410514
1105	13	0.4871077037083964
1105	14	0.7542563851022379
1105	15	0.636792205617418
1105	16	0.5601673485064254
1105	17	0.6013561817321147
1105	18	0.8596875721775419
1105	19	0.540894865214085
1105	20	0.5451656951561858
1105	21	0.5235198867985744
1105	22	0.5629647415039898
1105	23	0.7159474379889801
1105	24	0.6034050263928918
1105	25	0.6247572038462863
1105	26	0.5388226058517986
1105	27	0.4307842000457427
1105	28	0.5729367382022161
1105	29	0.5582717286357166
1105	30	0.7515537065115794
1105	31	0.8279869747602859
1105	32	0.6110855659484002
1105	33	0.58652193958644
1105	34	0.45661519154317176
1105	35	0.5437135283372427
1105	36	0.49269593831544967
1105	37	0.448396964381061
1105	38	0.5512369446119223
1105	39	0.48944477034771694
1105	40	0.43827257849535783
1105	41	0.6132724843550506
1105	42	0.6037091666601455
1105	43	0.6832102665898319
1105	44	0.5688655322422278
1105	45	0.5557395757706871
1105	46	0.5136553310958192
1105	47	0.525523188233791
1105	48	0.7464272165809902
1105	49	0.6847864320046071
1105	50	0.7210677829577001
1106	1	0.18523070208811276
1106	2	0.12880025503379855
1106	3	0.16117645951754866
1106	4	0.16756516175658476
1106	5	0.19200542654154162
1106	6	0.12956438934838843
1106	7	0.13848878762414074
1106	8	0.22079419740806117
1106	9	-1.1906510562497488
1106	10	-0.6787782528563326
1106	11	-0.09768663847101067
1106	12	0.173335329370353
1106	13	0.266858035591774
1106	14	0.3833263082430207
1106	15	0.3177155109080445
1106	16	0.35719273615004
1106	17	0.41452783908309243
1106	18	-1.2520317367398874
1106	19	-0.15705730914807248
1106	20	-0.0451698991924232
1106	21	0.15786608611223585
1106	22	0.3645720326087556
1106	23	-1.1409891158564704
1106	24	-0.08885443945658977
1106	25	0.15174066132504294
1106	26	0.23521971052800694
1106	27	0.37428339855217274
1106	28	0.4906893425781007
1106	29	0.41848579819541243
1106	30	-0.5595761507498764
1106	31	0.1863659301023617
1106	32	0.2983259413304964
1106	33	0.3893090904393901
1106	34	0.35357286350084394
1106	35	0.3437880347204967
1106	36	0.45094185337626413
1106	37	0.3754904120276521
1106	38	0.37263385800088145
1106	39	0.31599936181554256
1106	40	0.34817360647303275
1106	41	0.510869916515027
1106	42	0.6890028117151772
1106	43	0.5628573033590669
1106	44	0.5812835178344713
1106	45	0.4316121325543065
1106	46	0.42456358207999756
1106	47	0.46457945346504204
1106	48	0.6673315653052967
1106	49	0.6411390815242797
1106	50	0.6034123525040052
1107	1	0.5806462828217214
1107	2	0.6750502185351444
1107	3	0.5759909568769288
1107	4	0.5824925930143152
1107	5	0.5924734570176824
1107	6	0.6072895672027911
1107	7	0.7068374378332933
1107	8	0.5045020461727105
1107	9	0.8501586769757026
1107	10	0.8211673052810795
1107	11	0.750155608225981
1107	12	0.9006598697463346
1107	13	0.8954746753985674
1107	14	1.111806655013197
1107	15	1.0394982841087028
1107	16	0.8120951882002245
1107	17	1.1201052101062743
1107	18	1.1154121236214394
1107	19	0.990833351246792
1107	20	0.8827004726605665
1107	21	0.8694655936865274
1107	22	0.9985325462460608
1107	23	1.059402432279839
1107	24	0.7586956296318235
1107	25	0.9377554133743828
1107	26	0.8706616456442333
1107	27	0.9069351411156319
1107	28	0.9193555263636526
1107	29	0.822730191295804
1107	30	1.0650765939959683
1107	31	1.1370283217955475
1107	32	0.9538537212798772
1107	33	0.8577469386483187
1107	34	0.8197908400531659
1107	35	0.7513218277641798
1107	36	0.7872587200869461
1107	37	0.8885897081452327
1107	38	0.964454674572309
1107	39	0.8684250858781866
1107	40	1.0297662287529405
1107	41	0.8527212977653633
1107	42	0.8708526670863179
1107	43	0.823086863406651
1107	44	0.8180038006307484
1107	45	0.8561528853025351
1107	46	0.9928953722659557
1107	47	0.9301055097360731
1107	48	0.9263917555880852
1107	49	0.9634447038239742
1107	50	1.0577220664204923
1108	1	-0.041885480348774706
1108	2	-0.01105727514664423
1108	3	-0.04672629763421554
1108	4	0.011760339054127655
1108	5	0.028402241064419798
1108	6	0.017927734118060812
1108	7	0.07719816657573375
1108	8	0.07967683177259488
1108	9	0.08708331473440722
1108	10	0.02156773459577721
1108	11	0.10841178757581105
1108	12	0.027825923128560248
1108	13	0.1692134979784825
1108	14	0.0914168257381931
1108	15	0.20262468219239338
1108	16	0.2365021818317814
1108	17	-0.08808202034431645
1108	18	0.22220967502349964
1108	19	0.15175892216818454
1108	20	0.024376102091707708
1108	21	0.1417052383962485
1108	22	0.1932715141883321
1108	23	-0.023804231265060594
1108	24	0.09952881220862508
1108	25	0.10433187556155588
1108	26	0.050220911483911834
1108	27	0.02483242699286592
1108	28	0.059950105152388744
1108	29	0.03016236067642808
1108	30	0.17670038126330923
1108	31	0.3556878152573333
1108	32	0.22962976822088166
1108	33	0.25036082874128596
1108	34	0.085110993617171
1108	35	-0.017005848535376344
1108	36	0.08523657449747901
1108	37	0.12006540915377428
1108	38	0.057594338607543705
1108	39	0.09674406662805524
1108	40	0.1712401842907667
1108	41	0.1693060008940961
1108	42	0.18452123680870613
1108	43	0.34012166599728033
1108	44	0.24184599511210791
1108	45	0.08036748428484941
1108	46	0.19903798863487765
1108	47	0.1621873549137547
1108	48	0.2149726916430552
1108	49	-0.007356732887373596
1108	50	0.09556997025609348
1109	1	-0.05386817578838077
1109	2	-0.0996347111303919
1109	3	-0.13976147265847416
1109	4	0.06429899846158804
1109	5	-0.02838625160279662
1109	6	-0.003657294349437734
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2210	45	-0.0823031578853346
2210	46	0.1689569274110454
2210	47	0.14471577662001778
2210	48	0.29606419232529646
2210	49	0.24194754176367184
2210	50	0.12868137732597873
2211	1	-0.1647231462262262
2211	2	-0.15422169644398664
2211	3	-0.2271850897429566
2211	4	-0.08711674610092501
2211	5	-0.24082439667319022
2211	6	-0.309706173147724
2211	7	-0.22641079894219018
2211	8	-0.28065202508442155
2211	9	-0.11465325044313346
2211	10	-0.17485651848007677
2211	11	-0.10608315484815023
2211	12	-0.14461269987768333
2211	13	-0.2103860247930882
2211	14	-0.1512964545591302
2211	15	-0.2898492752876294
2211	16	-0.2396411809532637
2211	17	-0.0643621390249649
2211	18	0.225562721967421
2211	19	-0.03643370920751465
2211	20	-0.08396426536912571
2211	21	-0.15830063804520478
2211	22	-0.19150415153079337
2211	23	0.11374764683569794
2211	24	0.06110649862925044
2211	25	0.017779655977264497
2211	26	-0.01875185676142621
2211	27	-0.06790233255551925
2211	28	-0.11276446687883634
2211	29	-0.09093159983117971
2211	30	0.1586546058605407
2211	31	0.07975535443564041
2211	32	-0.024051786243212803
2211	33	-0.05390433940572592
2211	34	-0.14931839985553808
2211	35	-0.16744211789592356
2211	36	-0.11559410699009476
2211	37	-0.13863400346386356
2211	38	-0.13024949753343407
2211	39	-0.07636813558146827
2211	40	-0.21194297482097113
2211	41	-0.0796651930787533
2211	42	0.0030678276467099863
2211	43	-0.10944673149352013
2211	44	0.01552925022377849
2211	45	0.001963208040827169
2211	46	-0.2966287902348128
2211	47	-0.1622653755237664
2211	48	-0.1756755340101286
2211	49	-0.21565803239334613
2211	50	-0.11378534259310058
2212	1	-0.17312716494888392
2212	2	-0.12630709122737827
2212	3	-0.3575101747452898
2212	4	-0.31550480468542474
2212	5	-0.20857963912393984
2212	6	-0.14181483838739695
2212	7	-0.22675932251643616
2212	8	-0.0885089268725352
2212	9	-0.1856473036692634
2212	10	-0.01772779234639188
2212	11	-0.13285683343674748
2212	12	-0.24919905680668109
2212	13	-0.0992234094418123
2212	14	-0.26420887336877286
2212	15	-0.2912047752987448
2212	16	-0.327734898002376
2212	17	-0.016184177971027225
2212	18	0.10314476555564056
2212	19	-0.15310961319505623
2212	20	-0.2499824666874062
2212	21	-0.009104935731336639
2212	22	-0.07538565026170119
2212	23	0.027038804839619957
2212	24	-0.002422497790973921
2212	25	-0.1012782475630516
2212	26	-0.25636702274306344
2212	27	-0.20327495360645287
2212	28	-0.3330459629343565
2212	29	-0.26414424603076014
2212	30	-0.05277686795129455
2212	31	-0.15970424783171921
2212	32	-0.2088669413063755
2212	33	-0.33503533094264526
2212	34	-0.31178520379855
2212	35	-0.3164048535312105
2212	36	-0.3349707161774873
2212	37	-1.578711448728214
2212	38	-0.9746533351250619
2212	39	-0.5056300067589845
2212	40	-0.4509423379700621
2212	41	-1.1553700539883813
2212	42	-0.632805389310839
2212	43	-0.3999366565772249
2212	44	-0.4341756164704762
2212	45	-0.34099908326305695
2212	46	-0.5685954917719123
2212	47	-0.4374664047529251
2212	48	-0.5166639128820094
2212	49	-0.40642520364188783
2212	50	-0.2932827564144098
2213	1	-0.6031329170340172
2213	2	-0.5839890009196402
2213	3	-0.7887008563673774
2213	4	-0.7575467949068795
2213	5	-0.5961666394115426
2213	6	-0.625458784317793
2213	7	-0.6224682670088129
2213	8	-0.5925095020810502
2213	9	-0.6596708872183044
2213	10	-0.8370720546414745
2213	11	-0.8712577537703723
2213	12	-0.7727308370770274
2213	13	-0.7631261992031071
2213	14	-2.588787533132937
2213	15	-1.5006669553390068
2213	16	-1.186259488166307
2213	17	-0.8147001400072682
2213	18	-0.8946205493710195
2213	19	-0.891404119953755
2213	20	-1.1626571862732575
2213	21	-1.113393115196395
2213	22	-0.9656042294966696
2213	23	-1.6620753740502252
2213	24	-1.2002999783776858
2213	25	-0.9900933180260907
2213	26	-1.1439827834563232
2213	27	-1.1094440649092974
2213	28	-0.8777655487842253
2213	29	-1.2536323104541154
2213	30	-1.0146434195762595
2213	31	-0.9754218204517926
2213	32	-1.01464491082033
2213	33	-1.0097146521965996
2213	34	-1.0100336938762098
2213	35	-1.1266205567236227
2213	36	-1.1073837158898812
2213	37	-1.1204255700972112
2213	38	-1.0042823256341222
2213	39	-1.0801453935867045
2213	40	-1.3143101838141789
2213	41	-1.5366786533556507
2213	42	-1.2487559213787165
2213	43	-1.4725193428009788
2213	44	-1.4605374226252081
2213	45	-1.401436241733002
2213	46	-1.4922250574894846
2213	47	-1.3426087688599146
2213	48	-1.4279092393128394
2213	49	-1.5491948326323985
2213	50	-1.4480483589453286
2214	1	-0.7304168632496442
2214	2	-0.6734872622211832
2214	3	-0.8214449575090015
2214	4	-0.8699582734066773
2214	5	-0.7008464122754852
2214	6	-0.6820479404488751
2214	7	-0.8696796228608434
2214	8	-0.7882803884488467
2214	9	-1.0086352028984393
2214	10	-1.2448400792280765
2214	11	-1.0723529793020177
2214	12	-1.024322239373582
2214	13	-0.927525450956658
2214	14	-1.4744071776921075
2214	15	-1.1986841497951417
2214	16	-0.836404027131934
2214	17	-1.484195929338334
2214	18	-1.5005442938358229
2214	19	-1.0932136498752953
2214	20	-1.095123618578107
2214	21	-1.1085797608513133
2214	22	-1.1247567239287974
2214	23	-0.8502082519705425
2214	24	-0.9267302866371523
2214	25	-0.9354381460221077
2214	26	-1.2846722730657305
2214	27	-1.0695657143791308
2214	28	-0.9870598249841436
2214	29	-0.8955966237573563
2214	30	-0.8335101161917962
2214	31	-0.7433311276583012
2214	32	-0.8723824398481526
2214	33	-0.9632628568092017
2214	34	-1.0735258750811525
2214	35	-1.0149979695688316
2214	36	-1.0835891461434741
2214	37	-1.0901455005968117
2214	38	-1.1239342056389745
2214	39	-0.971643383922256
2214	40	-0.9919205965144045
2214	41	-0.9398255193011664
2214	42	-0.840835965684029
2214	43	-0.9667529487641318
2214	44	-0.9922265152932006
2214	45	-0.9867645265420286
2214	46	-0.947074374251109
2214	47	-0.9160993100075245
2214	48	-1.0819738480640915
2214	49	-1.1210142266668655
2214	50	-1.0219352710501397
2215	1	-0.030249127562747447
2215	2	-0.15468539252739077
2215	3	-0.04016216061797806
2215	4	0.12343394466158486
2215	5	0.05836738887990833
2215	6	0.01984055799494655
2215	7	0.029561618483647578
2215	8	0.057754088879910626
2215	9	-0.09187313608440917
2215	10	-0.38796672720267367
2215	11	-0.1167325558047807
2215	12	-0.14470292407293783
2215	13	-0.24711246059221992
2215	14	-0.24101650233574645
2215	15	-0.12989074675983403
2215	16	-0.03947607771097239
2215	17	-0.08556935370688734
2215	18	0.11217627859244317
2215	19	-0.1784413162679499
2215	20	-0.10972890113400524
2215	21	-0.18333204833248118
2215	22	-0.5002983504491993
2215	23	-0.2839344443430605
2215	24	-0.14711366423597383
2215	25	-0.04944044731009001
2215	26	-0.13340886961507437
2215	27	-0.21181621153857577
2215	28	-0.20404563066543346
2215	29	-0.2322067288854086
2215	30	0.05373266056181336
2215	31	-0.13878628928475223
2215	32	-0.22003439126459767
2215	33	-0.15197280734656715
2215	34	-0.20015221519800264
2215	35	-0.24213016400347698
2215	36	-0.2304695134001875
2215	37	-0.1609723622233119
2215	38	-0.17687482903150453
2215	39	-0.28774130870090175
2215	40	-0.25174354065682536
2215	41	-0.26761678946881773
2215	42	-0.19216545927744022
2215	43	-0.30318098650306524
2215	44	-0.1825523424355282
2215	45	-0.253140207925469
2215	46	-0.1523049891404682
2215	47	-0.16700592710049733
2215	48	-0.2781480594895598
2215	49	-0.3234806943632549
2215	50	-0.2719462023962649
2216	1	-0.43199674346976025
2216	2	-0.7877652576284504
2216	3	-0.5342452339605721
2216	4	-1.830881589765557
2216	5	-0.7615676251291923
2216	6	-0.523355324562516
2216	7	-0.5424460193452205
2216	8	-0.33011113898943734
2216	9	-0.29846253054116545
2216	10	-0.8545728463010391
2216	11	-0.5917236555721033
2216	12	-0.6290106055064584
2216	13	-0.6463715974281641
2216	14	-0.562585469134369
2216	15	-0.6032855215826487
2216	16	-0.690816361801204
2216	17	-0.5458039976222153
2216	18	-0.31125952825198056
2216	19	-0.7240789549231863
2216	20	-0.7647728254064106
2216	21	-0.78772369599033
2216	22	-0.9806077615162607
2216	23	-2.7235804203724605
2216	24	-1.4487768253803424
2216	25	-1.002071979756053
2216	26	-1.1320625827401756
2216	27	-1.0659515065091796
2216	28	-1.3316456549883995
2216	29	-1.043425179834344
2216	30	-1.0121448608026427
2216	31	-0.7303280798400635
2216	32	-0.8498621503237374
2216	33	-1.0073386991602788
2216	34	-1.054995734815158
2216	35	-1.3094083351238979
2216	36	-1.1513873209857337
2216	37	-0.9719696716245068
2216	38	-1.1480408816470842
2216	39	-1.2762189245507471
2216	40	-1.286191572912827
2216	41	-1.4385736021616644
2216	42	-1.4556574064508574
2216	43	-1.5594107595874078
2216	44	-1.5200765549663267
2216	45	-1.4465049740221545
2216	46	-1.3294531934762972
2216	47	-1.4119779582817173
2216	48	-1.340081309563491
2216	49	-1.5762515641745516
2216	50	-1.6762489776577247
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	149465.0
3	7396.0
4	14722.0
5	11227.0
6	9008.0
7	7674.0
8	6793.0
9	6421.0
10	6218.0
11	5845.0
12	6019.0
13	6420.0
14	6576.0
15	7070.0
16	7763.0
17	8195.0
18	8941.0
19	10525.0
20	13486.0
21	18452.0
22	23360.0
23	26456.0
24	28491.0
25	29103.0
26	31444.0
27	35445.0
28	40941.0
29	45826.0
30	52687.0
31	62593.0
32	76446.0
33	94565.0
34	119857.0
35	153903.0
36	202158.0
37	277300.0
38	442535.0
39	859874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.38786031137068	18.02579341291726	13.120728929384967	24.465617346327093
2	15.507515255568016	20.319930174133052	39.003933185853136	25.168621384445792
3	18.60426560414777	25.25742013981185	29.284889249395068	26.85342500664531
4	12.583981656360987	16.501018691367864	37.99288851676002	32.92211113551113
5	13.871787123621266	37.87571065115071	34.23195543925213	14.02054678597589
6	31.83663221181513	37.020548226744346	17.6239031902708	13.518916371169729
7	28.01961635833589	32.32068683968261	21.822462298890883	17.837234503090617
8	26.286015119696742	34.575850752679486	20.06242158339964	19.075712544224135
9	26.69406128063484	16.038488096803892	19.210824126441317	38.056626496119954
10	14.70135160548754	28.944506313053026	33.617251442056215	22.73689063940322
11	34.38776604143497	23.35951604511697	23.871330804000944	18.381387109447115
12	23.540168239487087	25.155660577725463	30.09212886393813	21.212042318849317
13	27.676472391660823	22.407914825602607	25.855167762198977	24.060445020537593
14	22.275477818827063	21.363361542585352	27.205742561030128	29.15541807755746
15	23.623570571353493	29.0134231567869	23.76622651392934	23.59677975793027
16	23.476024294130404	28.251090505536204	25.27335007617181	22.99953512416159
17	22.520849878816826	27.82570590827081	26.49947477012199	23.153969442790373
18	22.486382161376483	27.69224866562138	27.26452126972621	22.55684790327592
19	23.311051064465342	27.276915825229548	27.303669620431815	22.108363489873295
20	23.633153764034702	26.64178460180702	27.103818097351134	22.621243536807135
21	23.93836812008994	26.547314910964104	27.10739294092013	22.406924028025827
22	23.563673785819727	26.32812235035853	26.929829700102125	23.178374163719624
23	23.349812117198855	26.603033050861104	26.870546369146453	23.176608462793588
24	23.17365952474986	26.505370395806583	27.12079853916739	23.20017154027617
25	23.269298298203996	26.351681669079042	27.116607713324026	23.26241231939294
26	23.272950662683563	26.533142058538445	27.163944586956422	23.029962691821563
27	23.105638196295196	26.68210420578726	27.075819695298748	23.1364379026188
28	23.189858375403354	26.6744313308655	27.087198960782278	23.04851133294887
29	23.249983553443087	26.78647298104808	26.876997571391765	23.086545894117066
30	23.383719480192017	26.69380908855455	26.898439287901983	23.024032143351455
31	23.56033641081859	26.348914886692587	26.80351484215755	23.287233860331273
32	23.206297576740326	26.818695254308476	26.884440617958454	23.09056655099274
33	23.426066007794645	26.425900193495693	26.838277614247424	23.30975618446224
34	23.44271251697721	26.551241627003225	26.954001825745756	23.052044030273812
35	23.668540730919318	26.570325374555892	26.83118689593976	22.92994699858503
36	23.609628774077674	26.584520735263535	26.737846254420315	23.06800423623848
37	23.521337086144	26.351741705747024	27.052138437320316	23.07478277078866
38	23.535759801570443	26.500178148082163	26.80563359050608	23.15842845984131
39	23.47152220115737	26.76339373637421	26.747637910571985	23.017446151896436
40	23.6589691840367	26.411216566714486	26.924952969723876	23.004861279524942
41	23.409366089242443	26.598426140791037	26.883399124872625	23.10880864509389
42	23.533117150247016	26.770211674229195	26.66710967415315	23.02956150137064
43	23.488711288642865	26.28713089987435	26.805685262638434	23.41847254884435
44	23.45843393092584	26.630571394325443	26.732110751528225	23.178883923220493
45	23.50371237962164	26.48063672105097	26.54032931957972	23.475321579747668
46	23.616436460871864	26.560036383611486	26.43808416175912	23.385442993757525
47	23.637039781556542	26.5942379853015	26.53068282757445	23.238039405567516
48	23.423762202623134	26.768609770866043	26.490959852924657	23.316668173586166
49	23.630384829710653	26.55896636203564	26.434399068125668	23.37624974012803
50	23.65233901425642	26.595610606764232	26.537287650328018	23.21476272865133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2387.0
1	1946.0
2	1505.0
3	1441.0
4	1377.0
5	1331.0
6	1285.0
7	1242.0
8	1199.0
9	1295.5
10	1392.0
11	1789.0
12	2186.0
13	3749.0
14	5312.0
15	7521.5
16	9731.0
17	10849.0
18	11967.0
19	12911.0
20	13855.0
21	16426.0
22	18997.0
23	23557.0
24	28117.0
25	34351.0
26	40585.0
27	47364.5
28	54144.0
29	60984.0
30	67824.0
31	75969.5
32	84115.0
33	92585.0
34	101055.0
35	110124.5
36	119194.0
37	127181.0
38	135168.0
39	143977.0
40	152786.0
41	161311.0
42	169836.0
43	176467.5
44	183099.0
45	189816.5
46	196534.0
47	202839.5
48	209145.0
49	206761.5
50	204378.0
51	200876.5
52	197375.0
53	192506.5
54	187638.0
55	179759.0
56	171880.0
57	159996.0
58	148112.0
59	135193.5
60	122275.0
61	106514.5
62	90754.0
63	77960.5
64	65167.0
65	55013.5
66	44860.0
67	36775.0
68	28690.0
69	23749.0
70	18808.0
71	14982.5
72	11157.0
73	9232.0
74	7307.0
75	5781.5
76	4256.0
77	3367.5
78	2479.0
79	1931.0
80	1383.0
81	1088.5
82	794.0
83	631.5
84	469.0
85	351.0
86	233.0
87	195.0
88	157.0
89	128.0
90	99.0
91	66.0
92	33.0
93	33.0
94	33.0
95	25.5
96	18.0
97	18.5
98	19.0
99	25.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06333013829932904
2	0.06634259893194577
3	0.0632616732849514
4	0.07733123373955908
5	0.08208955223880597
6	0.062440093112419555
7	0.0476516500068465
8	0.05809256469943858
9	0.05641517184718609
10	0.06610297138162399
11	0.05439545392304533
12	0.06942352457894016
13	0.06569218129535807
14	0.06658222648226757
15	0.07835820895522388
16	0.07329179789127756
17	0.08482815281391208
18	0.07021087224428317
19	0.0680542242913871
20	0.08092564699438587
21	0.07945364918526633
22	0.07438723812132
23	0.07164863754621388
24	0.0605573052170341
25	0.07616732849513899
26	0.08684787073805285
27	0.08013829932904286
28	0.09759687799534437
29	0.09092153909352321
30	0.07791318636176914
31	0.073599890455977
32	0.08089141448719704
33	0.07777625633301383
34	0.06315897576338492
35	0.08250034232507189
36	0.0880802409968506
37	0.09769957551691084
38	0.07805011639052443
39	0.05631247432561961
40	0.06175544296864302
41	0.055627824181843075
42	0.06028344515952348
43	0.039675475831849924
44	0.03936738326715049
45	0.04244830891414487
46	0.04121593865534712
47	0.03087772148432151
48	0.045734629604272214
49	0.05110913323291798
50	0.04285909900041079
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2921200.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.8737770017965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4440924988213	42.339167158790744
2	12.833026000591156	14.597253180317555
3	5.211651677986491	8.892189460345264
4	2.617875727033339	5.955539212708401
5	1.451292129566213	4.127023247070557
6	0.8902778223167753	3.038007740165357
7	0.5698606253327377	2.2687088289094905
8	0.39578188048454943	1.800768832962395
9	0.27533163795220406	1.409323516058972
>10	1.2494630977670789	11.914185791061401
>50	0.04547358635822771	1.7480994699231769
>100	0.015145624657134413	1.3600857791307068
>500	3.0320463865128133E-4	0.11377850504145565
>1k	4.244864940446078E-4	0.43586927751435334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3306	0.11317266876626043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.423250718882651E-5
7	0.0	0.0	0.0	0.0	3.423250718882651E-5
8	0.0	0.0	0.0	0.0	3.423250718882651E-5
9	0.0	0.0	0.0	0.0	3.423250718882651E-5
10	0.0	0.0	0.0	0.0	3.423250718882651E-5
11	0.0	0.0	0.0	0.0	3.423250718882651E-5
12	0.0	0.0	0.0	0.0	3.423250718882651E-5
13	0.0	0.0	0.0	0.0	3.423250718882651E-5
14	0.0	0.0	0.0	0.0	6.846501437765302E-5
15	0.0	0.0	0.0	3.423250718882651E-5	6.846501437765302E-5
16	0.0	0.0	0.0	3.423250718882651E-5	6.846501437765302E-5
17	0.0	0.0	0.0	3.423250718882651E-5	1.0269752156647952E-4
18	0.0	0.0	0.0	3.423250718882651E-5	1.3693002875530603E-4
19	0.0	0.0	0.0	3.423250718882651E-5	1.3693002875530603E-4
20	0.0	0.0	0.0	3.423250718882651E-5	1.3693002875530603E-4
21	0.0	0.0	0.0	3.423250718882651E-5	1.3693002875530603E-4
22	0.0	0.0	0.0	6.846501437765302E-5	1.3693002875530603E-4
23	0.0	0.0	0.0	1.3693002875530603E-4	1.3693002875530603E-4
24	0.0	0.0	0.0	1.7116253594413254E-4	1.7116253594413254E-4
25	0.0	0.0	0.0	2.0539504313295905E-4	1.7116253594413254E-4
26	0.0	0.0	0.0	2.7386005751061206E-4	2.0539504313295905E-4
27	0.0	0.0	0.0	4.107900862659181E-4	2.0539504313295905E-4
28	0.0	0.0	0.0	8.215801725318362E-4	2.3962755032178558E-4
29	0.0	0.0	0.0	0.001540462823497193	2.3962755032178558E-4
30	0.0	0.0	0.0	0.002225112967273723	2.3962755032178558E-4
31	0.0	0.0	0.0	0.004176365877036834	2.3962755032178558E-4
32	0.0	0.0	0.0	0.006641106394632343	2.3962755032178558E-4
33	0.0	0.0	0.0	0.009927427084759688	2.3962755032178558E-4
34	0.0	0.0	0.0	0.013111050253320553	2.7386005751061206E-4
35	0.0	0.0	0.0	0.016328905929070246	2.7386005751061206E-4
36	0.0	0.0	0.0	0.021052991921128302	2.7386005751061206E-4
37	0.0	0.0	0.0	0.026735588114473505	3.0809256469943857E-4
38	0.0	0.0	0.0	0.03351362453786115	3.0809256469943857E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5880	0.0	20.01806	1
GGTATCA	3080	0.0	16.358004	1
TATCAAC	7135	0.0	16.342276	2
TCAACGC	7130	0.0	16.292025	4
ATCAACG	7155	0.0	16.26501	3
CAACGCA	7180	0.0	16.11701	5
GAGTACT	3480	0.0	15.869738	12
AACGCAG	7380	0.0	15.677279	6
GTACATG	8040	0.0	14.776896	1
AGAGTAC	7155	0.0	14.391433	11
AGTACTT	3815	0.0	14.360848	13
TACATGG	8080	0.0	14.322044	2
GTACTTT	3925	0.0	13.901607	14
ACGCAGA	8530	0.0	13.435684	7
ACATGGG	8400	0.0	13.38289	3
CGCAGAG	8715	0.0	13.150701	8
CATGGGG	5520	0.0	12.714001	4
CAGAGTA	8670	0.0	12.460563	10
GTCCTAG	640	0.0	12.37565	1
TCGATCG	125	0.002673287	12.316006	41
>>END_MODULE