FastQCFastQC Report
Mon 6 Feb 2017
SRR2077449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921200
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43730.14969875393673832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71100.021.0757161
GGTATCA37450.018.4494191
TATACCG1053.7733902E-516.7639275
GCGATAC1259.440073E-615.84163914
TATCAAC95500.015.5044732
ATCAACG97050.015.3483173
TGCGATA1452.2856875E-615.17398513
TCAACGC98200.015.1461714
CAACGCA99800.014.9036015
TAGCGCG900.00436045214.66692929
CCGTCTT3450.014.66215944
AACGCAG101850.014.5589266
GTATAGA5700.014.2834031
GTACATG70750.013.3735541
GTACGGG1000.00875938713.2011411
TACATGG70100.013.1504722
TATACTC3350.013.1359135
AGAGTAC106500.012.87059611
GGACCGA2401.6623744E-812.8335636
GAGTACT72950.012.57682312
Produced by FastQC (version 0.11.5)