##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077449_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2921200 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.50802033410927 31.0 31.0 34.0 27.0 34.0 2 30.466440846227577 31.0 31.0 34.0 26.0 34.0 3 30.63435677118992 31.0 31.0 34.0 27.0 34.0 4 34.10003080925647 37.0 35.0 37.0 30.0 37.0 5 33.7437823497193 37.0 35.0 37.0 28.0 37.0 6 33.69229357798165 37.0 35.0 37.0 28.0 37.0 7 33.51175646994386 37.0 35.0 37.0 28.0 37.0 8 33.477800219088046 37.0 35.0 37.0 27.0 37.0 9 34.92202656442558 39.0 35.0 39.0 27.0 39.0 10 34.787183349308506 39.0 35.0 39.0 27.0 39.0 11 34.72961317266876 39.0 35.0 39.0 27.0 39.0 12 34.667401752704365 39.0 35.0 39.0 27.0 39.0 13 34.633775503217855 39.0 35.0 39.0 27.0 39.0 14 35.777134739148295 40.0 36.0 41.0 27.0 41.0 15 35.78212481172121 40.0 36.0 41.0 27.0 41.0 16 35.724862727646176 40.0 36.0 41.0 27.0 41.0 17 35.70244796658907 40.0 36.0 41.0 27.0 41.0 18 35.70109886348076 40.0 36.0 41.0 27.0 41.0 19 35.740808229494725 40.0 36.0 41.0 27.0 41.0 20 35.74026119402985 40.0 36.0 41.0 27.0 41.0 21 35.680867794057235 40.0 36.0 41.0 27.0 41.0 22 35.68146686293304 40.0 36.0 41.0 27.0 41.0 23 35.614402985074626 40.0 36.0 41.0 26.0 41.0 24 35.59928693687526 40.0 36.0 41.0 26.0 41.0 25 35.57724770642202 40.0 36.0 41.0 26.0 41.0 26 35.49361974531015 40.0 35.0 41.0 26.0 41.0 27 35.43241647268246 40.0 35.0 41.0 25.0 41.0 28 35.37274373545119 40.0 35.0 41.0 25.0 41.0 29 35.29341126934137 40.0 35.0 41.0 25.0 41.0 30 35.16075790770916 39.0 35.0 41.0 24.0 41.0 31 35.06992708475969 39.0 35.0 41.0 24.0 41.0 32 34.9803276050938 39.0 35.0 41.0 24.0 41.0 33 34.879607353142546 39.0 35.0 41.0 24.0 41.0 34 34.83817814596741 39.0 35.0 41.0 24.0 41.0 35 34.76800869505683 39.0 34.0 41.0 23.0 41.0 36 34.65697487333972 39.0 34.0 41.0 23.0 41.0 37 34.562006709571406 39.0 34.0 41.0 22.0 41.0 38 34.47892783787485 39.0 34.0 41.0 21.0 41.0 39 34.382126865671644 39.0 34.0 41.0 19.0 41.0 40 34.28506606873887 39.0 34.0 41.0 19.0 41.0 41 34.22149596056415 39.0 34.0 41.0 18.0 41.0 42 34.09738566342599 39.0 33.0 41.0 16.0 41.0 43 34.183305490894156 39.0 34.0 41.0 16.0 41.0 44 34.108293509516635 39.0 34.0 41.0 15.0 41.0 45 34.02709708339039 39.0 34.0 41.0 14.0 41.0 46 33.86281014651513 39.0 33.0 41.0 8.0 41.0 47 33.697491441873204 39.0 33.0 41.0 2.0 41.0 48 33.53149767218951 39.0 33.0 41.0 2.0 41.0 49 33.37484937696837 39.0 33.0 41.0 2.0 41.0 50 33.222591400794194 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7698399699125424 1101 2 0.9128911328685803 1101 3 0.8485856470774529 1101 4 0.8228935250733258 1101 5 0.8583171515193371 1101 6 0.8253843782543626 1101 7 0.8933533018700572 1101 8 0.8349094985040608 1101 9 0.9171910955382216 1101 10 0.887271240644047 1101 11 1.056929035775191 1101 12 1.0465821948820064 1101 13 1.0376648170509668 1101 14 1.1173520381399626 1101 15 1.1095038902251062 1101 16 1.2159167267282385 1101 17 1.2554926832050484 1101 18 1.2388405069073016 1101 19 1.312786009307004 1101 20 1.1546162993320124 1101 21 1.2382699197648819 1101 22 1.2756017793258962 1101 23 1.3347278384031753 1101 24 1.327210153988652 1101 25 1.3687292646207894 1101 26 1.3973222902226539 1101 27 1.3381384154833569 1101 28 1.3844826633078071 1101 29 1.4467906786503661 1101 30 1.49402551452512 1101 31 1.5226658888067064 1101 32 1.5054788047547518 1101 33 1.5247730532277401 1101 34 1.516511047071475 1101 35 1.504421966781642 1101 36 1.4878651009761796 1101 37 1.4446209177580869 1101 38 1.4347061202915938 1101 39 1.4811301469021245 1101 40 1.552276823105359 1101 41 1.5490743487911445 1101 42 1.4716049765053043 1101 43 1.536403886774572 1101 44 1.5510277725604311 1101 45 1.5528673303146618 1101 46 1.5832847441387088 1101 47 1.6541651127290606 1101 48 1.6490712749533074 1101 49 1.6485618191470053 1101 50 1.7983834492828592 1102 1 0.7251748056220393 1102 2 0.8259550766564132 1102 3 0.7229144841419668 1102 4 0.7767540431067985 1102 5 0.8117069104725019 1102 6 0.7560228081055129 1102 7 0.7602783872133259 1102 8 0.8705136936221649 1102 9 0.9558809969193902 1102 10 0.9450390954162202 1102 11 0.9433049926021155 1102 12 0.871094329216902 1102 13 0.9071752322838549 1102 14 1.0101195107451346 1102 15 0.9731261277824999 1102 16 0.9720034571780829 1102 17 0.9874068182036879 1102 18 1.043124445714227 1102 19 1.0053634947707621 1102 20 1.0238350102076268 1102 21 1.085580563030149 1102 22 0.9889088758943245 1102 23 1.0437413107902174 1102 24 1.0831761809789384 1102 25 1.0934171957764818 1102 26 1.1210914617692893 1102 27 1.0462650837618312 1102 28 1.133529467100459 1102 29 1.102649319570979 1102 30 1.158780358940028 1102 31 1.1737567690550037 1102 32 1.1632695323908493 1102 33 1.1981205498624092 1102 34 1.1742778730324162 1102 35 1.173669079082572 1102 36 1.1759297942893667 1102 37 1.2310370034191962 1102 38 1.2336489699833635 1102 39 1.178060179372551 1102 40 1.3387318788890141 1102 41 1.1828659759615974 1102 42 1.196138741856224 1102 43 1.1714381932126443 1102 44 1.2389386637899875 1102 45 1.121574280345044 1102 46 1.2084326942089376 1102 47 1.218223706542588 1102 48 1.2577331485027443 1102 49 1.2914803009770353 1102 50 1.2973374652137437 1103 1 0.4391432266194961 1103 2 0.6547988329428023 1103 3 0.49163424109174514 1103 4 0.49781792572120764 1103 5 0.5259686051219603 1103 6 0.5320509464987211 1103 7 0.5224883138470773 1103 8 0.5132817042909537 1103 9 0.6112054251082881 1103 10 0.665393940759536 1103 11 0.6702610650129728 1103 12 0.6367266977937476 1103 13 0.6984437281034985 1103 14 0.7391778611080682 1103 15 0.7270582363824047 1103 16 0.7543199058097301 1103 17 0.8066133570000531 1103 18 0.730102836031179 1103 19 0.7574316897749895 1103 20 0.6959816822488563 1103 21 0.850590711072023 1103 22 0.8234086458503356 1103 23 0.8073103315457786 1103 24 0.8246872878470342 1103 25 0.8570057070419708 1103 26 0.8037299821719088 1103 27 0.7998872270980968 1103 28 0.9330268256783683 1103 29 0.8819841781851423 1103 30 0.8942904243126222 1103 31 0.8598080115902746 1103 32 0.9336047625460324 1103 33 0.7949732447103912 1103 34 0.8209976227559466 1103 35 0.8528979306961233 1103 36 0.7955511705239431 1103 37 0.8980874923383837 1103 38 0.8460646080082554 1103 39 0.8390006224300137 1103 40 0.8669899377186994 1103 41 0.8793018944981839 1103 42 0.825313521299222 1103 43 0.7795220017946676 1103 44 0.8502112826412613 1103 45 0.822511743339156 1103 46 0.8860829671854873 1103 47 0.8840735189833211 1103 48 0.8116463161945617 1103 49 0.8250045632777727 1103 50 0.8767661021874247 1104 1 0.4648092022714181 1104 2 0.6621300872011737 1104 3 0.5234676078591392 1104 4 0.5035040850742334 1104 5 0.4718250117670735 1104 6 0.5837676545752402 1104 7 0.572290383551227 1104 8 0.4791381069597662 1104 9 0.5564794031443938 1104 10 0.5004741954256744 1104 11 0.6134377930783543 1104 12 0.6226538249305875 1104 13 0.5156501145987136 1104 14 0.7278719466196009 1104 15 0.6705303538677683 1104 16 0.6876949041446494 1104 17 0.7390611814766075 1104 18 0.6935177405174642 1104 19 0.672419976587932 1104 20 0.8004839776677031 1104 21 0.6969205161676868 1104 22 0.7255219700156417 1104 23 0.7765307838062157 1104 24 0.7413144068921014 1104 25 0.7770501084009922 1104 26 0.7103389116305507 1104 27 0.731985435357231 1104 28 0.7211013936384063 1104 29 0.6879532413747143 1104 30 0.7359803341122344 1104 31 0.720669306102721 1104 32 0.7864418330384879 1104 33 0.7801566545510568 1104 34 0.7173220002014915 1104 35 0.6763747441923229 1104 36 0.8114994157924897 1104 37 0.8885527711834129 1104 38 0.7984491556275373 1104 39 0.8146751073648986 1104 40 0.7871186366473424 1104 41 0.8306784499523658 1104 42 0.7920265681971017 1104 43 0.82472935691667 1104 44 0.7902789413816365 1104 45 0.8161166096151788 1104 46 0.7110653571448537 1104 47 0.8937346864927349 1104 48 0.8134563778649948 1104 49 0.8869678496980455 1104 50 0.8155234705206809 1105 1 0.37749939912310637 1105 2 0.5090688627113771 1105 3 0.44932506839569086 1105 4 0.40130716765992247 1105 5 0.41957633451839627 1105 6 0.46415040348801284 1105 7 0.49616097848807783 1105 8 0.493469282264023 1105 9 0.5207171916575462 1105 10 0.48325378068972924 1105 11 0.6040224327313197 1105 12 0.578128043603563 1105 13 0.6494164230544612 1105 14 0.6448480768462517 1105 15 0.6247585024540925 1105 16 0.7372063351260607 1105 17 0.6343159822697118 1105 18 0.6274349466913947 1105 19 0.6134575832570093 1105 20 0.6597629549591062 1105 21 0.7563402582996446 1105 22 0.6053460413510976 1105 23 0.62622049866156 1105 24 0.6678154420887878 1105 25 0.5180428017746408 1105 26 0.5290165437139791 1105 27 0.6991357415344481 1105 28 0.6916968782090507 1105 29 0.7027294417099199 1105 30 0.7154283935292369 1105 31 0.6727449028470573 1105 32 0.7501442222067141 1105 33 0.7147366056941564 1105 34 0.6589018527544326 1105 35 0.690052759681933 1105 36 0.7328052972433028 1105 37 0.7369238426393707 1105 38 0.6975270637634168 1105 39 0.6600610878440492 1105 40 0.7290202765303491 1105 41 0.656681878699068 1105 42 0.5959033524051449 1105 43 0.6972714494380625 1105 44 0.6881655517423653 1105 45 0.7482194829706401 1105 46 0.795227121689642 1105 47 0.7333011088748904 1105 48 0.68296545363701 1105 49 0.7311584244786857 1105 50 0.7728763794781486 1106 1 0.34105803057761364 1106 2 0.46864431979225785 1106 3 0.43437807409222273 1106 4 0.43553342986883337 1106 5 0.40547855995423987 1106 6 0.356082863786348 1106 7 0.32425084141163296 1106 8 0.25238441576339454 1106 9 0.413930798302097 1106 10 0.3146715635174928 1106 11 0.350018025473112 1106 12 0.2755757934916545 1106 13 0.3548190444549135 1106 14 0.3204593352485432 1106 15 0.34493882894317096 1106 16 0.39038744206710874 1106 17 0.4806735861291216 1106 18 0.38545562101720776 1106 19 0.3108748228289855 1106 20 0.36105357002412575 1106 21 0.3942853359048826 1106 22 0.4145672344479294 1106 23 0.4729798057426322 1106 24 0.390943218144983 1106 25 0.3768531098618837 1106 26 0.37065203055914964 1106 27 0.3260772114621844 1106 28 0.32703069358624504 1106 29 0.35413339446962766 1106 30 0.4299313144855219 1106 31 0.3595964868967627 1106 32 0.41445633413270855 1106 33 0.47163461515904714 1106 34 0.45240592908569255 1106 35 0.34834898328907826 1106 36 0.43640207137514153 1106 37 0.38560529963608303 1106 38 0.4510845283657119 1106 39 0.4857350884638194 1106 40 0.5761449422558869 1106 41 0.4967201170200255 1106 42 0.5580420586147099 1106 43 0.5528111281519941 1106 44 0.49879299304587477 1106 45 0.5028062642566269 1106 46 0.6231763884856178 1106 47 0.6158750318320401 1106 48 0.518643107843225 1106 49 0.5584997803992167 1106 50 0.6104753228073889 1107 1 0.4687973840381119 1107 2 0.6010141514651757 1107 3 0.41988815572675975 1107 4 0.48809412830706833 1107 5 0.5456417725139246 1107 6 0.5712744982007578 1107 7 0.5570644967117531 1107 8 0.5448180329542183 1107 9 0.562284685479348 1107 10 0.7066419539259243 1107 11 0.7009370851170971 1107 12 0.684987454034399 1107 13 0.672222826939894 1107 14 0.6748786864107998 1107 15 0.6904665429594985 1107 16 0.7808437270410593 1107 17 0.8101782499506243 1107 18 0.7635067907166331 1107 19 0.76336939983549 1107 20 0.7300135138114854 1107 21 0.7942604697254296 1107 22 0.6992707664814972 1107 23 0.8610236003592959 1107 24 0.762402212967352 1107 25 0.8333896655004267 1107 26 0.8010523477913196 1107 27 0.8415117620766281 1107 28 0.7182081919544032 1107 29 0.7741640439196047 1107 30 0.8837696070832166 1107 31 0.9142542331355159 1107 32 0.8071190215827286 1107 33 0.9215789959205338 1107 34 0.83065715850163 1107 35 0.9676583251547726 1107 36 0.9807682334027916 1107 37 0.9845664773134075 1107 38 0.859621728264365 1107 39 1.0257797157207307 1107 40 0.8875594605254591 1107 41 0.9560867076125064 1107 42 0.8968567518894446 1107 43 0.813543569002988 1107 44 0.8623257989775865 1107 45 0.8754012855577074 1107 46 0.8839164587325783 1107 47 0.9009983690240233 1107 48 0.9830886155889473 1107 49 0.9538088321594884 1107 50 1.0449925939702496 1108 1 0.2118163898382015 1108 2 0.35133204976129306 1108 3 0.2936896880367712 1108 4 0.21973062589380987 1108 5 0.16102917253521554 1108 6 0.21382061800814967 1108 7 0.06558699597886175 1108 8 0.1210630184675594 1108 9 0.13742411266936472 1108 10 0.19461017924992063 1108 11 0.14716031117725237 1108 12 0.09160318089386266 1108 13 0.018176524634469615 1108 14 0.08658508888342453 1108 15 0.10236984874632071 1108 16 0.02297400201537414 1108 17 0.03461846926256129 1108 18 0.10832733556166829 1108 19 0.10891011504445203 1108 20 0.04342409809891734 1108 21 0.038343127585299897 1108 22 -0.02247622707148622 1108 23 0.10145077859168339 1108 24 0.047875350584718035 1108 25 0.05814156264749215 1108 26 -0.026243895630187808 1108 27 0.097354178062524 1108 28 -0.01831796213754444 1108 29 0.008745766091173834 1108 30 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0.2554793049670607 1109 17 0.32231159584701174 1109 18 0.2666115356656604 1109 19 0.33187145923284334 1109 20 0.2601816589438144 1109 21 0.3283382082692725 1109 22 0.3267158684366578 1109 23 0.2936502396244336 1109 24 0.33827573546258805 1109 25 0.31011659751560927 1109 26 0.2621395883570017 1109 27 0.25252016505038455 1109 28 0.215590704834284 1109 29 0.24249327339332893 1109 30 0.18761950399804306 1109 31 0.23206230428635877 1109 32 0.23795443649644454 1109 33 0.2565722125272316 1109 34 0.1836179663678621 1109 35 0.25071097733307113 1109 36 0.34399736582030016 1109 37 0.31626617362559983 1109 38 0.12877979022352548 1109 39 0.2610257529210074 1109 40 0.2497778376900328 1109 41 0.10865770921561335 1109 42 0.07854165585781203 1109 43 0.062197597495213586 1109 44 0.03709256423791629 1109 45 0.08688831474771774 1109 46 0.010890116500334557 1109 47 0.07025210351430644 1109 48 0.12710246043208429 1109 49 0.10351492904307946 1109 50 0.032426349637624696 1110 1 0.16957259945318626 1110 2 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-2.0556820719256024 2216 15 -2.114300136245454 2216 16 -2.231664130896462 2216 17 -2.074828428893028 2216 18 -2.1203820877774433 2216 19 -2.092252195013444 2216 20 -2.1789616218784005 2216 21 -2.132504735424 2216 22 -2.1443086936924374 2216 23 -2.006180750319679 2216 24 -2.1607064570126013 2216 25 -2.195594967459961 2216 26 -2.5447486263241004 2216 27 -2.642696875062903 2216 28 -2.6627690290057444 2216 29 -2.379014821126468 2216 30 -2.4283593923462163 2216 31 -2.2610667567570744 2216 32 -2.452452930241847 2216 33 -2.372975106584171 2216 34 -2.2563116873971936 2216 35 -2.4488148367760516 2216 36 -2.553165152793518 2216 37 -2.539711350538667 2216 38 -2.554538302033997 2216 39 -2.571294604507184 2216 40 -2.6175985509577195 2216 41 -2.7010312880160825 2216 42 -2.6824214770588632 2216 43 -2.5134325503942208 2216 44 -2.3791686490118416 2216 45 -2.2563092532941056 2216 46 -2.054254718814981 2216 47 -2.2395566105157663 2216 48 -2.3179717408985034 2216 49 -2.301710864891348 2216 50 -2.2606795088944835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72573.0 3 11166.0 4 19235.0 5 11601.0 6 8690.0 7 6825.0 8 5825.0 9 5099.0 10 4997.0 11 4918.0 12 5159.0 13 5326.0 14 5933.0 15 6454.0 16 6941.0 17 7470.0 18 8140.0 19 9157.0 20 10637.0 21 13302.0 22 16879.0 23 20739.0 24 23677.0 25 26949.0 26 29376.0 27 33274.0 28 38923.0 29 44971.0 30 51466.0 31 61313.0 32 75064.0 33 93698.0 34 116820.0 35 149055.0 36 192728.0 37 259727.0 38 409004.0 39 1048089.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.89593723187244 18.708276526525328 14.535809345510248 25.85997689609199 2 14.603834480473289 20.605146280028126 38.135745237024246 26.655274002474343 3 17.604703013257264 25.008292850416147 29.89708649656938 27.48991763975721 4 12.143611006794949 16.907879489555622 37.172016003335685 33.77649350031375 5 13.51964165242614 37.96241185998966 34.40157256504125 14.116373922542952 6 31.96620383844955 37.39173757468883 17.178243078977555 13.463815507884059 7 27.6639341455564 32.26782131092529 21.901499119977537 18.16674542354077 8 26.559621169191576 34.272923218623795 19.996815684636363 19.170639927548265 9 26.497587287064217 15.200159980057283 19.383713811018076 38.918538921860424 10 14.368464342679548 28.90358816104777 33.878204008730535 22.849743487542142 11 34.615753411522334 22.877027667507733 23.88901217216921 18.618206748800727 12 23.53697597238998 24.85484225021138 30.458419389205638 21.149762388193004 13 27.96161481328777 21.89488420098836 26.183853168407932 23.959647817315936 14 22.150510458308034 20.81743876674944 27.74399379730913 29.2880569776334 15 23.52408569953112 28.747847717724667 24.13100532985224 23.59706125289197 16 23.168652248467193 27.978329892084396 25.68248202805032 23.170535831398094 17 22.189360055343602 27.400700014383855 27.094323855969645 23.3156160743029 18 22.11787583621046 27.095564903767944 28.194244330824432 22.592314929197165 19 23.042363387468153 26.749557933412767 28.303289701483898 21.90478897763518 20 23.354404715752096 26.04345467472352 28.015497706098397 22.58664290342599 21 23.57495536648457 25.95155093118202 28.075534326452594 22.397959375880813 22 23.15972750199661 25.791041484300404 28.02837584778907 23.020855165913915 23 22.910433014574462 25.972929183200794 27.979010750449234 23.137627051775514 24 22.88437045918191 25.939224825952785 28.117036712840676 23.05936800202463 25 22.808536956218 25.971095550021506 28.04999410979883 23.170373383961667 26 22.970419299646714 26.055064157270586 28.08461061118927 22.88990593189343 27 22.75185220953512 26.241649903117303 28.08903071411008 22.917467173237497 28 22.79321087539379 26.215272961891056 28.134196203249083 22.85731995946607 29 23.028700132975306 26.212470775739522 27.80793475271832 22.950894338566847 30 23.045410385827502 26.217394684114844 27.8613624930698 22.875832436987857 31 23.072484532859306 25.86425762690041 27.886192253125873 23.17706558711441 32 22.960728075618178 26.29980728376951 27.815273206417302 22.92419143419501 33 23.075390181218296 25.968562258137634 27.791496579772552 23.164550980871514 34 23.1059287084087 26.108921532273776 27.904547373481204 22.88060238583632 35 23.337899704412784 26.05344674277133 27.776159023387788 22.8324945294281 36 23.268141026299478 26.13023395957666 27.57199743887365 23.029627575250206 37 23.150136797383407 25.89152828578208 27.955010077915105 23.00332483891941 38 23.256532162473267 26.00374841390871 27.629042606880127 23.110676816737893 39 23.179132173400983 26.28195120615161 27.59060687605705 22.94830974439035 40 23.358903439896554 25.97890645942179 27.76104257838698 22.901147522294675 41 23.12852811174792 26.1049372424472 27.55149515423298 23.2150394915719 42 23.277235291680945 26.29628653308077 27.409905522631327 23.01657265260695 43 23.16812831163745 25.895900844123076 27.557547777612694 23.378423066626773 44 23.224889647206552 26.24684791889044 27.275814542947387 23.252447890955626 45 23.242923115369738 26.124410758850168 27.158668049693098 23.473998076086993 46 23.444196256643302 26.210788466969625 26.989345040950987 23.355670235436083 47 23.46844950174415 26.29349682834735 26.965072453349492 23.272981216559003 48 23.246754224830905 26.475892292914306 26.909997391466877 23.367356090787908 49 23.494691900166917 26.26081919871176 26.898948239008952 23.34554066211237 50 23.490726898720418 26.285648559097986 26.889169520240202 23.334455021941395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 844.0 1 582.0 2 320.0 3 515.0 4 710.0 5 787.0 6 864.0 7 834.5 8 805.0 9 1077.0 10 1349.0 11 2071.5 12 2794.0 13 5266.5 14 7739.0 15 10715.0 16 13691.0 17 15723.0 18 17755.0 19 18757.5 20 19760.0 21 21798.5 22 23837.0 23 27168.0 24 30499.0 25 36150.0 26 41801.0 27 48126.5 28 54452.0 29 60322.5 30 66193.0 31 74276.5 32 82360.0 33 90926.5 34 99493.0 35 108475.5 36 117458.0 37 125920.5 38 134383.0 39 142648.0 40 150913.0 41 159743.0 42 168573.0 43 175145.0 44 181717.0 45 188785.5 46 195854.0 47 202190.5 48 208527.0 49 206803.0 50 205079.0 51 202234.5 52 199390.0 53 193822.5 54 188255.0 55 179995.5 56 171736.0 57 159675.5 58 147615.0 59 133930.0 60 120245.0 61 104541.0 62 88837.0 63 76244.5 64 63652.0 65 53513.0 66 43374.0 67 35442.0 68 27510.0 69 22474.0 70 17438.0 71 13865.5 72 10293.0 73 8536.0 74 6779.0 75 5288.0 76 3797.0 77 2905.5 78 2014.0 79 1593.5 80 1173.0 81 888.5 82 604.0 83 481.5 84 359.0 85 281.0 86 203.0 87 144.0 88 85.0 89 62.5 90 40.0 91 28.0 92 16.0 93 13.5 94 11.0 95 6.5 96 2.0 97 1.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016500068465014377 2 7.531151581541832E-4 3 3.0809256469943857E-4 4 0.002019717924140764 5 0.038682733123373954 6 0.025674380391619882 7 0.028070655894837735 8 0.021737642064904833 9 0.028618376009858962 10 0.029919211283034368 11 0.03751882787895385 12 0.037689990414897984 13 0.034609064767903604 14 0.0402916609612488 15 0.03573873750513488 16 0.04220868136382309 17 0.0431329590579214 18 0.04556346706832808 19 0.04761741749965767 20 0.04395453923045324 21 0.0448103519101739 22 0.04285909900041079 23 0.042071751335067784 24 0.039675475831849924 25 0.03751882787895385 26 0.040668218540325894 27 0.04018896343968232 28 0.0402916609612488 29 0.0379638504724086 30 0.03454059975352595 31 0.0334451595234835 32 0.028892236067369577 33 0.02170340955771601 34 0.0187251814322881 35 0.019615226619197592 36 0.02088182938518417 37 0.015644255785293715 38 0.0170477885800356 39 0.011639052444201013 40 0.007223059016842394 41 0.006743803916198823 42 0.006264548815555252 43 0.0021224154457072436 44 0.003902505819526222 45 0.0023962755032178556 46 8.558126797206626E-4 47 3.4232507188826513E-4 48 6.161851293988771E-4 49 1.3693002875530606E-4 50 3.0809256469943857E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2921200.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.28096753553201 #Duplication Level Percentage of deduplicated Percentage of total 1 72.02502962652004 39.09588294902865 2 14.104206777982181 15.311799804601625 3 5.574082035657399 9.07699698053734 4 2.8207722551046963 6.124569888178696 5 1.6185799904980123 4.392904395894216 6 0.9995027779706176 3.255238670561829 7 0.6462319769164065 2.4554667871595495 8 0.4373201984460332 1.8990530795585234 9 0.3164071957377093 1.5457399847862585 >10 1.3839762786189762 12.575508069291907 >50 0.0556447907787024 2.032744978674036 >100 0.017358723697688032 1.5656683181436215 >500 5.070697552020277E-4 0.18437798206910452 >1k 3.8030231638085273E-4 0.48404811151457583 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4373 0.14969875393673832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.423250718882651E-5 0.0 9 0.0 0.0 0.0 3.423250718882651E-5 0.0 10 0.0 0.0 0.0 3.423250718882651E-5 0.0 11 0.0 0.0 0.0 3.423250718882651E-5 0.0 12 0.0 0.0 0.0 3.423250718882651E-5 0.0 13 0.0 0.0 0.0 3.423250718882651E-5 0.0 14 0.0 0.0 0.0 3.423250718882651E-5 0.0 15 0.0 3.423250718882651E-5 0.0 3.423250718882651E-5 0.0 16 0.0 3.423250718882651E-5 0.0 3.423250718882651E-5 0.0 17 0.0 3.423250718882651E-5 0.0 3.423250718882651E-5 0.0 18 0.0 3.423250718882651E-5 0.0 3.423250718882651E-5 0.0 19 0.0 3.423250718882651E-5 0.0 3.423250718882651E-5 0.0 20 0.0 3.423250718882651E-5 0.0 3.423250718882651E-5 0.0 21 0.0 3.423250718882651E-5 0.0 3.423250718882651E-5 0.0 22 0.0 3.423250718882651E-5 0.0 6.846501437765302E-5 0.0 23 3.423250718882651E-5 3.423250718882651E-5 0.0 1.7116253594413254E-4 3.423250718882651E-5 24 3.423250718882651E-5 3.423250718882651E-5 0.0 1.7116253594413254E-4 3.423250718882651E-5 25 3.423250718882651E-5 3.423250718882651E-5 0.0 2.0539504313295905E-4 3.423250718882651E-5 26 3.423250718882651E-5 3.423250718882651E-5 0.0 3.0809256469943857E-4 3.423250718882651E-5 27 3.423250718882651E-5 3.423250718882651E-5 0.0 4.107900862659181E-4 3.423250718882651E-5 28 3.423250718882651E-5 3.423250718882651E-5 0.0 8.215801725318362E-4 3.423250718882651E-5 29 3.423250718882651E-5 3.423250718882651E-5 0.0 0.0014719978091195399 3.423250718882651E-5 30 3.423250718882651E-5 3.423250718882651E-5 0.0 0.002225112967273723 3.423250718882651E-5 31 3.423250718882651E-5 3.423250718882651E-5 0.0 0.004279063398603314 3.423250718882651E-5 32 3.423250718882651E-5 3.423250718882651E-5 0.0 0.006949198959331782 3.423250718882651E-5 33 3.423250718882651E-5 3.423250718882651E-5 0.0 0.010372449678214433 3.423250718882651E-5 34 3.423250718882651E-5 3.423250718882651E-5 0.0 0.013590305353964124 3.423250718882651E-5 35 3.423250718882651E-5 3.423250718882651E-5 0.0 0.01715048610160208 3.423250718882651E-5 36 3.423250718882651E-5 3.423250718882651E-5 0.0 0.022011502122415445 3.423250718882651E-5 37 3.423250718882651E-5 3.423250718882651E-5 0.0 0.028002190880460087 3.423250718882651E-5 38 3.423250718882651E-5 3.423250718882651E-5 0.0 0.03491715733260304 3.423250718882651E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7110 0.0 21.075716 1 GGTATCA 3745 0.0 18.449419 1 TATACCG 105 3.7733902E-5 16.763927 5 GCGATAC 125 9.440073E-6 15.841639 14 TATCAAC 9550 0.0 15.504473 2 ATCAACG 9705 0.0 15.348317 3 TGCGATA 145 2.2856875E-6 15.173985 13 TCAACGC 9820 0.0 15.146171 4 CAACGCA 9980 0.0 14.903601 5 TAGCGCG 90 0.004360452 14.666929 29 CCGTCTT 345 0.0 14.662159 44 AACGCAG 10185 0.0 14.558926 6 GTATAGA 570 0.0 14.283403 1 GTACATG 7075 0.0 13.373554 1 GTACGGG 100 0.008759387 13.20114 11 TACATGG 7010 0.0 13.150472 2 TATACTC 335 0.0 13.135913 5 AGAGTAC 10650 0.0 12.870596 11 GGACCGA 240 1.6623744E-8 12.833563 6 GAGTACT 7295 0.0 12.576823 12 >>END_MODULE