FastQCFastQC Report
Mon 6 Feb 2017
SRR2077448_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077448_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3950410
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.11057080151174181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.025.431451
GTATCAA54150.024.8627851
TATCAAC71150.018.7360482
TCAACGC72500.018.2984
ATCAACG72650.018.25863
CAACGCA73400.018.1331235
AACGCAG77300.017.1033336
GTACATG101350.015.3024921
TTATACT10100.015.0297594
GTGCCGA4300.014.83820411
ACGCAGA88950.014.7405487
TACATGG102650.014.7223822
CGTGCCG4250.014.495090510
CGCAGAG90350.014.4390918
GCACCGT5550.014.2690586
TACCGCG3100.014.1939617
TATACTG9300.013.9566885
CGCACGA4000.013.750435
GTGTACG4350.013.6543581
ACATGGG107800.013.6519013
Produced by FastQC (version 0.11.5)