FastQCFastQC Report
Mon 6 Feb 2017
SRR2077448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3950410
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54110.13697312430861608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.027.8625221
GTATCAA65800.025.0123631
TATCAAC96700.016.9508842
ATCAACG98500.016.641123
TCAACGC98850.016.6046684
CAACGCA99950.016.3567145
GCACCGT6300.016.063956
AACGCAG103300.015.8029686
CGTGCCG3850.015.42979210
GTACATG103000.014.547521
CGCACGA3250.014.21309135
TACATGG103550.014.1084892
CGTCGTA2052.9296643E-813.95232310
CCTATAC5550.013.8751493
TAATACC4650.013.7218634
CTATACT7950.013.5611654
ACATGGG106350.013.5301563
ACGCAGA120600.013.4808017
GTAATAC4250.013.460063
CGCGATC2155.4707925E-813.30337823
Produced by FastQC (version 0.11.5)