FastQCFastQC Report
Mon 6 Feb 2017
SRR2077447_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077447_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3592980
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44590.12410311217986184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.027.5765841
GTATCAA59400.024.7775421
TCAACGC77800.018.3239884
TATCAAC78850.018.3024232
ATCAACG78300.018.2902493
CAACGCA78800.017.896025
AACGCAG81100.017.4148866
TATACCG1451.2511737E-716.689735
GTCCGTA1004.9474253E-415.3998531
GTACATG95950.014.949311
ACGCAGA94100.014.9392847
CGCAGAG96450.014.4840528
TACATGG97000.014.3561652
CGAACTA1251.6724055E-414.0800625
GAGTACT53900.013.91881712
GGACCGT2703.274181E-1113.8513346
AGAGTAC96700.013.81016711
ACATGGG101400.013.4726823
AGTACTT56200.013.34918513
GTATTAC4800.013.291541
Produced by FastQC (version 0.11.5)