FastQCFastQC Report
Mon 6 Feb 2017
SRR2077447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3592980
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55890.15555332899153348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.028.615491
GTATCAA69700.025.9170571
CGAATCG1057.7730874E-820.94821237
TATACCG951.5961048E-518.5295945
TATCAAC101300.017.8096562
ATCAACG102300.017.7648533
TCAACGC102550.017.6145174
CAACGCA105350.017.1895625
AACGCAG108500.016.6888836
GTACATG92900.014.8973931
ACGCAGA125900.014.2421957
TACATGG94750.014.18773652
ACCGTAT1251.6717368E-414.0807268
CGCAGAG129000.013.8487778
ACGACCG1606.338929E-613.7524325
CGTACAC3200.013.7510923
CTAGCGT1302.3306713E-413.5397254
GAGTACT89050.013.24378812
AGAGTAC129500.013.18442511
TAAGGCG3350.013.1366525
Produced by FastQC (version 0.11.5)