FastQCFastQC Report
Mon 6 Feb 2017
SRR2077446_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077446_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131028
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32810.10478986454289134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.024.9393221
GTATCAA47150.024.8687971
TATCAAC64250.017.8385662
TCAACGC63650.017.800494
ATCAACG64350.017.673823
CAACGCA64300.017.6544825
AACGCAG66200.017.146966
ACGCAGA73450.015.3952787
CGCAGAG75650.014.7733148
TTATACT7850.014.2930094
GAGTACT38200.014.28342212
GTACATG80200.014.1541771
GCGATAC1901.7545608E-713.89541814
GTACTTT40350.013.685925514
AGTACTT41500.013.67777913
AGAGTAC74750.013.62698811
GTATAAC3101.8189894E-1213.4834541
TACATGG81000.013.4707582
TGCGATA1851.9684794E-613.08172313
GTCTTAT5550.013.0806771
Produced by FastQC (version 0.11.5)