FastQCFastQC Report
Mon 6 Feb 2017
SRR2077446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3131028
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37750.12056743024974545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51700.024.981391
GGTATCA21950.023.3555681
TACGCGT851.434965E-418.11234742
ATACGCG851.434965E-418.11234741
TATCAAC73600.017.2784672
TCAACGC75450.016.8259144
CAACGCA75450.016.6806435
ATCAACG76500.016.6234663
CGCGTAT800.001993408716.4949144
AACGCAG78800.016.109576
GTACCGA2650.015.7740096
CCTACGC1452.286497E-615.173553
GCACCGT3550.014.8736396
CGAATCG2254.292815E-1014.6642537
ACGCGTA1100.001020889613.99590443
ACGCAGA91950.013.662147
TATACCG1453.83519E-513.6568495
TACCGTA1952.4088695E-713.5388267
GTACATG75800.013.4104031
TATTGCG1658.714034E-613.334975
Produced by FastQC (version 0.11.5)