FastQCFastQC Report
Mon 6 Feb 2017
SRR2077445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006492
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41150.022.9919531
GGTATCA20650.019.711921
GCGATAC1751.9281288E-1017.59986714
ATCAACG53950.017.086633
TATCAAC54750.017.0384482
TCAACGC54000.016.949434
CAACGCA54100.016.7944185
AACGCAG56550.016.0657396
CGCGCTA850.002981137715.52955316
GTACATG79000.015.0677161
CGTACAC2950.014.9156383
TACATGG78800.014.8257482
TGCGATA1951.5175829E-814.66728813
GAGTACT25750.014.35400612
ACGCAGA64350.013.9825687
AGAGTAC60050.013.92232111
ACATGGG81300.013.9093373
GTATAGG4950.013.779511
CCGTACA2256.724804E-913.6896962
GTATAAT7050.013.4201251
Produced by FastQC (version 0.11.5)