FastQCFastQC Report
Mon 6 Feb 2017
SRR2077445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006492
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50000.022.311551
GGTATCA24800.021.9147781
TATCAAC68250.016.3124052
TCAACGC69850.015.7812524
CAACGCA69850.015.7510635
ATCAACG70550.015.6558563
AACGCAG71650.015.4149896
TATGCGA2152.1645974E-1015.34974812
TACCGTA2601.8189894E-1215.231427
ACCGTAT2750.015.2006498
CGCACGA2153.5543053E-914.32476335
GTACCGT3400.014.2363766
CCGTATG2950.014.1700979
GCGAATC2358.221832E-1014.04338715
GTATGCG2358.221832E-1014.04338711
AGCGTAC2959.094947E-1213.42430223
GTACATG76900.013.3051081
ACGGTTG2858.0035534E-1113.12162236
GCACCGT4700.013.107386
CGTACAA2901.05501385E-1012.89710325
Produced by FastQC (version 0.11.5)