##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077445_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3006492 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.633615190062038 31.0 31.0 34.0 27.0 34.0 2 30.56032645355451 31.0 31.0 34.0 27.0 34.0 3 30.744559440038422 31.0 31.0 34.0 27.0 34.0 4 34.21793372475297 37.0 35.0 37.0 30.0 37.0 5 33.863816368046216 37.0 35.0 37.0 28.0 37.0 6 33.81531632214554 37.0 35.0 37.0 29.0 37.0 7 33.64005226024217 37.0 35.0 37.0 28.0 37.0 8 33.61300246267078 37.0 35.0 37.0 28.0 37.0 9 35.09157350160918 39.0 35.0 39.0 28.0 39.0 10 34.94066107609799 39.0 35.0 39.0 27.0 39.0 11 34.90884559147339 39.0 35.0 39.0 27.0 39.0 12 34.83028559530509 39.0 35.0 39.0 27.0 39.0 13 34.80157206471861 39.0 35.0 39.0 27.0 39.0 14 35.96984059827866 40.0 36.0 41.0 27.0 41.0 15 35.966035831793334 40.0 36.0 41.0 27.0 41.0 16 35.90605163758959 40.0 36.0 41.0 27.0 41.0 17 35.87006418111207 40.0 36.0 41.0 27.0 41.0 18 35.859904167381785 40.0 36.0 41.0 27.0 41.0 19 35.88739234962208 40.0 36.0 41.0 27.0 41.0 20 35.88230702094002 40.0 36.0 41.0 27.0 41.0 21 35.82455599416197 40.0 36.0 41.0 27.0 41.0 22 35.81964595282476 40.0 36.0 41.0 27.0 41.0 23 35.75749877265597 40.0 36.0 41.0 27.0 41.0 24 35.73738064162486 40.0 36.0 41.0 27.0 41.0 25 35.71671735697284 40.0 36.0 41.0 27.0 41.0 26 35.634927683160306 40.0 36.0 41.0 26.0 41.0 27 35.577805961233224 40.0 36.0 41.0 26.0 41.0 28 35.51018130099797 40.0 35.0 41.0 26.0 41.0 29 35.43724546747505 40.0 35.0 41.0 25.0 41.0 30 35.31531166555574 40.0 35.0 41.0 25.0 41.0 31 35.24513153535749 39.0 35.0 41.0 25.0 41.0 32 35.16621830359103 39.0 35.0 41.0 24.0 41.0 33 35.07531402045973 39.0 35.0 41.0 24.0 41.0 34 35.05079873819721 39.0 35.0 41.0 24.0 41.0 35 34.985052014108135 39.0 35.0 41.0 24.0 41.0 36 34.8780778395552 39.0 35.0 41.0 24.0 41.0 37 34.791684461492 39.0 35.0 41.0 23.0 41.0 38 34.71522924391616 39.0 34.0 41.0 23.0 41.0 39 34.62954965454756 39.0 34.0 41.0 22.0 41.0 40 34.53544762467354 39.0 34.0 41.0 21.0 41.0 41 34.48077693205237 39.0 34.0 41.0 21.0 41.0 42 34.371672035049485 39.0 34.0 41.0 20.0 41.0 43 34.48083281113005 39.0 34.0 41.0 21.0 41.0 44 34.42147958484506 39.0 34.0 41.0 19.0 41.0 45 34.36732377801105 39.0 34.0 41.0 18.0 41.0 46 34.219326377718616 39.0 34.0 41.0 15.0 41.0 47 34.08404080237034 39.0 34.0 41.0 13.0 41.0 48 33.93233409568361 39.0 34.0 41.0 7.0 41.0 49 33.7822751565612 39.0 34.0 41.0 2.0 41.0 50 33.63767241023758 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.763902645488681 1101 2 0.87004646903317 1101 3 0.8039296896503281 1101 4 0.7921499466056545 1101 5 0.847700107696447 1101 6 0.9368145554131999 1101 7 0.9007563027781913 1101 8 0.8067380340297348 1101 9 0.8814521726223816 1101 10 0.9766159898814735 1101 11 1.0932026456058566 1101 12 1.057445385000122 1101 13 1.0870190899657501 1101 14 1.127010454729927 1101 15 1.1425013621881988 1101 16 1.1804299762944481 1101 17 1.3182681671641419 1101 18 1.180287808584815 1101 19 1.1717245016305995 1101 20 1.1572918687974365 1101 21 1.1333480272113405 1101 22 1.2525130425214854 1101 23 1.2947802187371948 1101 24 1.2851289075297387 1101 25 1.284524857556299 1101 26 1.2192163846223636 1101 27 1.2759469655972282 1101 28 1.344098479158447 1101 29 1.390846904496442 1101 30 1.460247138112834 1101 31 1.415194778030667 1101 32 1.436751077489312 1101 33 1.387522868614731 1101 34 1.2679280955550496 1101 35 1.2589949521054962 1101 36 1.27797647743553 1101 37 1.401042344811394 1101 38 1.3720361807539234 1101 39 1.3767095394717046 1101 40 1.4034662883754478 1101 41 1.4157745917253592 1101 42 1.4461963748107678 1101 43 1.412357033085776 1101 44 1.50035812625417 1101 45 1.5396483849475544 1101 46 1.5411221857075006 1101 47 1.5846819261374776 1101 48 1.675278555371996 1101 49 1.6478980154523626 1101 50 1.7202146052365421 1102 1 0.6644169155105821 1102 2 0.7598584570572022 1102 3 0.6370565022880541 1102 4 0.6815504114446682 1102 5 0.6848781196054361 1102 6 0.7128395790235373 1102 7 0.7239693182705906 1102 8 0.7449101584244033 1102 9 0.7413601425351075 1102 10 0.8604558012666814 1102 11 0.9699067092633484 1102 12 1.0194960350379674 1102 13 0.9785291469654638 1102 14 0.9886580210230704 1102 15 1.0501465574004882 1102 16 1.0321578512299823 1102 17 1.0564660117578768 1102 18 1.0113194966738206 1102 19 1.0616664302436547 1102 20 1.111566445318374 1102 21 1.0417771526575876 1102 22 1.0226781474451627 1102 23 0.9995616305349913 1102 24 1.0223574221212672 1102 25 1.1175069940742972 1102 26 0.9961263438317332 1102 27 0.935390096495631 1102 28 0.8837674655548753 1102 29 1.0702451590058502 1102 30 1.0509798288877263 1102 31 1.098910543039139 1102 32 1.100004548711489 1102 33 1.0952891630976183 1102 34 1.0756846110039078 1102 35 1.0019598748444238 1102 36 0.9882992525378356 1102 37 0.96722724892998 1102 38 0.97130476259305 1102 39 0.9927953806360748 1102 40 1.102034128749466 1102 41 1.095235807138728 1102 42 0.9954249697779147 1102 43 1.0102099323014428 1102 44 1.0456461723897732 1102 45 0.8292229969677294 1102 46 0.909200739482138 1102 47 0.9837353692281923 1102 48 1.0668107156677777 1102 49 1.1020543058494852 1102 50 1.135003183860647 1103 1 0.4340739184815696 1103 2 0.5928629102500587 1103 3 0.46087379129356876 1103 4 0.47551409473054207 1103 5 0.5297817564015048 1103 6 0.6454731386738501 1103 7 0.6245409931257555 1103 8 0.6053270950114111 1103 9 0.6218151066822344 1103 10 0.6831630113919474 1103 11 0.74081770190881 1103 12 0.7492617332326432 1103 13 0.6859189242006991 1103 14 0.7636224309585913 1103 15 0.8508327879810054 1103 16 0.8686031284671927 1103 17 0.8524185863708666 1103 18 0.8218839050325286 1103 19 0.8597422935178827 1103 20 0.7207370529315114 1103 21 0.7381741530268258 1103 22 0.7861111925276631 1103 23 0.8697083138983928 1103 24 0.7877231187391232 1103 25 0.7628247029072242 1103 26 0.762189518988464 1103 27 0.7922390650604783 1103 28 0.7876025200273205 1103 29 0.7738290791558597 1103 30 0.8776416950238755 1103 31 0.8236498229858427 1103 32 0.765461832093024 1103 33 0.8443879467150168 1103 34 0.741670811230712 1103 35 0.8283324476966385 1103 36 0.7911445655162197 1103 37 0.9123170814851989 1103 38 0.870155657502167 1103 39 0.8503168614770971 1103 40 0.8325569091501563 1103 41 0.822145575123308 1103 42 0.7585116408158612 1103 43 0.8231312609796646 1103 44 0.8313685186403035 1103 45 0.79753555355245 1103 46 0.8810389528438165 1103 47 0.8741594409102689 1103 48 0.9010942824794697 1103 49 0.9433965446925185 1103 50 0.9216397158102367 1104 1 0.566921110087069 1104 2 0.7475132544338727 1104 3 0.6276422389127383 1104 4 0.5467697871464665 1104 5 0.6781099532642259 1104 6 0.7178840143051772 1104 7 0.8094299177773578 1104 8 0.8132432972642363 1104 9 0.8481318875567183 1104 10 0.8421796360561231 1104 11 0.855731711791762 1104 12 0.9186897946491825 1104 13 0.8851725781419901 1104 14 1.040053939771461 1104 15 0.984692077955863 1104 16 1.0511656750551808 1104 17 1.0258032038422513 1104 18 1.0685184790838278 1104 19 0.8387687882828487 1104 20 0.9141552316011357 1104 21 0.919433791239392 1104 22 0.921016031926392 1104 23 0.9897708796085567 1104 24 1.0069863674219164 1104 25 0.9579126297179528 1104 26 0.9754386148156371 1104 27 1.0564783783919154 1104 28 1.0745331216333156 1104 29 0.9588109120717192 1104 30 1.0031901826843637 1104 31 1.014322363576099 1104 32 0.9176997229387638 1104 33 1.1346686510351276 1104 34 1.0511540016168794 1104 35 1.0247470963621481 1104 36 1.1742276737433386 1104 37 1.06407977358527 1104 38 1.0959849504478356 1104 39 1.1555047528888593 1104 40 1.158373086834814 1104 41 1.2220952557626248 1104 42 1.2176668833864923 1104 43 1.208724728070301 1104 44 1.1890755855121213 1104 45 1.1641135749246132 1104 46 1.206159950305313 1104 47 1.281307727293317 1104 48 1.283911414029106 1104 49 1.2786138454963307 1104 50 1.257728426783025 1105 1 0.2732434206653025 1105 2 0.42103540629301506 1105 3 0.3091867353698561 1105 4 0.31960397752715863 1105 5 0.3644633880371728 1105 6 0.40756643908489565 1105 7 0.42117346251570353 1105 8 0.38610215345008214 1105 9 0.40869209922983885 1105 10 0.38416929667489796 1105 11 0.481831470078788 1105 12 0.40154497479595364 1105 13 0.40792529391267607 1105 14 0.46789186279151096 1105 15 0.5291501064669077 1105 16 0.5157726278332646 1105 17 0.5888359539544084 1105 18 0.4689121191191674 1105 19 0.5204798087143558 1105 20 0.4438173517760404 1105 21 0.4722382754112786 1105 22 0.4933210990811361 1105 23 0.508889559666251 1105 24 0.49210555866948624 1105 25 0.4642170069450984 1105 26 0.2725701127509197 1105 27 0.44884609907490614 1105 28 0.4582392124785457 1105 29 0.4825618780001406 1105 30 0.5866201833337783 1105 31 0.5987571680995956 1105 32 0.6159225338038752 1105 33 0.6131340328159212 1105 34 0.5713594070029586 1105 35 0.5517490034936046 1105 36 0.6375080947540752 1105 37 0.6680452486075694 1105 38 0.5759722839015211 1105 39 0.5476472640465602 1105 40 0.5863370199463986 1105 41 0.6263978897012166 1105 42 0.5680860531259597 1105 43 0.624948869935622 1105 44 0.6107005854376482 1105 45 0.5760200093037398 1105 46 0.7111923107692419 1105 47 0.6561725568937149 1105 48 0.5886350944532168 1105 49 0.6536720097288935 1105 50 0.7222066884038085 1106 1 0.42539858529273644 1106 2 0.5300103591945842 1106 3 0.41271538319870515 1106 4 0.4570611693828184 1106 5 0.4503583144987502 1106 6 0.4162112028216214 1106 7 0.5014210451460386 1106 8 0.4484547614472305 1106 9 0.5195561366203307 1106 10 0.43707114061793106 1106 11 0.5326070620699497 1106 12 0.6081514386096529 1106 13 0.5602481850792316 1106 14 0.5777794539606447 1106 15 0.6004085372263432 1106 16 0.6113142097383033 1106 17 0.6320566647924935 1106 18 0.5837219549064372 1106 19 0.5950838352001924 1106 20 0.48846174723306746 1106 21 0.6142453531410155 1106 22 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0.42015793180596717 1107 10 0.5515887999483553 1107 11 0.5637628090503881 1107 12 0.6070708246130607 1107 13 0.6129257598850444 1107 14 0.6939688414266811 1107 15 0.5108903967676923 1107 16 0.6040516753857474 1107 17 0.6269938486912494 1107 18 0.5490806263766927 1107 19 0.511959503968427 1107 20 0.6071427053325706 1107 21 0.5903482914873237 1107 22 0.5416840799063678 1107 23 0.6258987160232792 1107 24 0.7114614683640426 1107 25 0.6144304805361145 1107 26 0.6112825146054846 1107 27 0.6721624826633601 1107 28 0.5735228149109233 1107 29 0.6505481201659791 1107 30 0.6538902842168 1107 31 0.6345438459495938 1107 32 0.615807205642831 1107 33 0.6326294429880974 1107 34 0.6739368166625681 1107 35 0.5952714051151915 1107 36 0.6960854811150625 1107 37 0.6696537269748006 1107 38 0.7271890542470416 1107 39 0.688221807976106 1107 40 0.60971514487359 1107 41 0.6989783281812194 1107 42 0.7246251016103855 1107 43 0.6405341894761705 1107 44 0.7802012377989627 1107 45 0.6778172064799506 1107 46 0.7723773949937467 1107 47 0.7599451856768269 1107 48 0.6999992868066087 1107 49 0.8584322086990923 1107 50 0.8963187198103455 1108 1 0.2820023777950347 1108 2 0.4663288320009755 1108 3 0.3698836139712327 1108 4 0.39936855792082326 1108 5 0.3343469731429849 1108 6 0.30710698018508964 1108 7 0.2653693359642588 1108 8 0.2504241283227415 1108 9 0.25487399281427514 1108 10 0.33567457350058305 1108 11 0.3106504663267984 1108 12 0.40755480293567103 1108 13 0.4882487272517295 1108 14 0.35582560123955176 1108 15 0.44634296708117205 1108 16 0.40539809759561507 1108 17 0.3682065079531185 1108 18 0.3728876042114493 1108 19 0.4458272083663033 1108 20 0.41128980108445745 1108 21 0.42900945182511663 1108 22 0.35672432791808006 1108 23 0.3830361435541647 1108 24 0.39834980636791784 1108 25 0.3232372503708092 1108 26 0.27457154392593 1108 27 0.43769862172841556 1108 28 0.31620778369200764 1108 29 0.42410351247300326 1108 30 0.5693594378078259 1108 31 0.5114418465017394 1108 32 0.41664090770354534 1108 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-1.9297180651097179 2216 41 -1.9654728982800407 2216 42 -2.0133151708322856 2216 43 -1.7835548471199019 2216 44 -1.7277510679750208 2216 45 -1.7089765475842285 2216 46 -1.6382315452015987 2216 47 -1.6676920915054296 2216 48 -1.69703536851722 2216 49 -1.698631340027518 2216 50 -1.7100912078555908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70396.0 3 11306.0 4 19169.0 5 11636.0 6 8556.0 7 6892.0 8 5742.0 9 5316.0 10 5100.0 11 4874.0 12 5010.0 13 5322.0 14 5808.0 15 6259.0 16 6855.0 17 7360.0 18 8190.0 19 8809.0 20 10332.0 21 12925.0 22 15779.0 23 19343.0 24 22670.0 25 26108.0 26 28396.0 27 32772.0 28 37751.0 29 43954.0 30 51114.0 31 61318.0 32 75078.0 33 92836.0 34 117725.0 35 150772.0 36 197202.0 37 268656.0 38 428531.0 39 1110630.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.91765391570714 17.910003872260642 13.766119449260744 26.406222762771474 2 14.929567064707298 19.782755697116592 38.24472962873157 27.04294760944454 3 17.909083047284533 24.338894654213565 29.20475107101993 28.547271227481975 4 12.29281850651705 16.56407516975328 36.46061663997935 34.68248968375032 5 14.108877781023917 37.258782714943514 34.18103083974262 14.451308664289957 6 32.91608526087237 36.45149828023659 17.130094495492816 13.502321963398217 7 28.64432139212739 31.38964202333854 21.539486296686803 18.426550287847267 8 26.88948618831034 34.21903544239027 19.7613508238041 19.130127545495284 9 26.809032786972516 14.965444319639712 18.678233581227474 39.5472893121603 10 14.515570922743281 28.59414853513747 33.74417491976478 23.14610562235447 11 35.51985098534543 22.380048593471223 23.307663487217095 18.79243693396625 12 23.73672438427563 24.238700545232756 30.17087686444248 21.853698206049135 13 28.558737126085422 21.153397602115394 25.704906403442607 24.58295886835658 14 22.55073196783634 20.30463305110548 26.938645805430824 30.20598917562736 15 24.067470530554825 28.223155578906113 23.461927376746065 24.247446513793 16 24.133007411114544 27.295675252627433 24.89361829565662 23.677699040601407 17 22.878497569844914 27.24296027030858 26.268735287795188 23.60980687205132 18 22.90377938831898 26.74722626447432 27.093575076462624 23.255419270744074 19 23.917710345607894 26.356416566416918 26.990627272258354 22.73524581571683 20 23.97027749215508 25.914652610702365 26.947220253366744 23.16784964377581 21 24.336078224403995 25.78608085327001 26.753104224887174 23.12473669743882 22 23.90041811348129 25.56921167390143 26.85602953581584 23.67434067680144 23 23.75150364618602 25.501340179256253 26.721993081979704 24.02516309257802 24 23.467890384484843 25.7715451666873 26.796445441804188 23.964119007023665 25 23.60979862377384 25.635839111974768 26.891445836050735 23.862916428200656 26 23.53794003718186 26.009265849624104 26.966231192365044 23.486562920828995 27 23.582748104557545 26.02408796649846 26.737043858466976 23.65612007047702 28 23.598518325276316 25.923750718742678 26.85062929914603 23.627101656834977 29 23.771329661181063 25.89828976728617 26.68842140709049 23.64195916444228 30 23.731324859028703 25.915161347960787 26.734786296262158 23.618727496748352 31 23.80366142606027 25.505341811734116 26.793971333990584 23.897025428215027 32 23.49579343199121 26.007257269746713 26.70869574764984 23.788253550612236 33 23.824689822711633 25.70549883707429 26.54611230824867 23.923699031965402 34 23.944001216294485 25.798683966703607 26.66599242610495 23.591322390896956 35 23.92333434580986 25.715584142954146 26.619527179006404 23.74155433222959 36 23.863949018309693 25.94576791087852 26.447799826002193 23.74248324480959 37 23.85806745654846 25.588976474873874 26.70150884832839 23.851447220249273 38 23.846332469696183 25.7871207614911 26.68918244567669 23.677364323136025 39 24.009712345450257 25.869282528589643 26.37422735524211 23.74677777071799 40 23.933293394171255 25.593607161639508 26.890809413356685 23.582290030832546 41 23.735264502982275 25.751689557227397 26.591237420836052 23.921808518954276 42 24.06602055167257 25.76759419294835 26.510406895441402 23.655978359937677 43 23.826330783776637 25.594788459971298 26.533683918168045 24.045196838084024 44 23.918217297546498 25.89692441716004 26.286665223059003 23.89819306223446 45 23.88610020566146 25.789940583362743 26.217894709181788 24.10606450179401 46 23.932448083425744 25.80737864956038 26.18386819306972 24.076305073944155 47 23.954789594195997 25.826582350898526 26.090346305774602 24.128281749130874 48 23.812376391417697 26.138396376343522 26.104170145280204 23.945057086958577 49 24.054475665895 25.757364093557918 26.213977807934864 23.974182432612224 50 24.114245382301405 25.717617569539886 26.202204840484338 23.965932207674367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 378.0 1 277.0 2 176.0 3 320.0 4 464.0 5 514.0 6 564.0 7 552.0 8 540.0 9 733.0 10 926.0 11 1341.0 12 1756.0 13 3135.0 14 4514.0 15 6367.5 16 8221.0 17 9503.5 18 10786.0 19 11932.5 20 13079.0 21 14567.0 22 16055.0 23 19304.0 24 22553.0 25 27347.0 26 32141.0 27 38261.5 28 44382.0 29 50901.0 30 57420.0 31 64675.5 32 71931.0 33 81458.5 34 90986.0 35 100902.5 36 110819.0 37 119970.0 38 129121.0 39 139039.5 40 148958.0 41 159257.5 42 169557.0 43 176638.5 44 183720.0 45 192759.5 46 201799.0 47 209620.0 48 217441.0 49 218901.5 50 220362.0 51 221789.5 52 223217.0 53 218853.0 54 214489.0 55 204935.5 56 195382.0 57 183356.5 58 171331.0 59 155086.0 60 138841.0 61 121864.5 62 104888.0 63 89400.5 64 73913.0 65 61583.5 66 49254.0 67 40802.5 68 32351.0 69 25719.5 70 19088.0 71 14895.0 72 10702.0 73 8774.5 74 6847.0 75 5086.0 76 3325.0 77 2621.0 78 1917.0 79 1477.0 80 1037.0 81 791.0 82 545.0 83 447.0 84 349.0 85 274.5 86 200.0 87 141.0 88 82.0 89 65.5 90 49.0 91 32.5 92 16.0 93 15.0 94 14.0 95 8.0 96 2.0 97 3.0 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016497632456697043 2 4.989203363920476E-4 3 3.991362691136381E-4 4 0.002328294903162889 5 0.04037928589199638 6 0.02813910697251149 7 0.030101526962320203 8 0.024646664617767152 9 0.030201311029598617 10 0.03173133339453423 11 0.038250559123390315 12 0.03891578623857972 13 0.036354661845100535 14 0.041144297074464195 15 0.0362881391335816 16 0.04244148994908352 17 0.04437064858313277 18 0.047530477380282404 19 0.04979224957192635 20 0.04530196654439793 21 0.046366329928700954 22 0.04413781909281648 23 0.04313997842003238 24 0.04071189944959108 25 0.03898230895009865 26 0.04200909232421041 27 0.04180952418965359 28 0.04137712656478048 29 0.03891578623857972 30 0.036354661845100535 31 0.03495768490320281 32 0.03173133339453423 33 0.023648823944983056 34 0.021320529041820167 35 0.021985756157009564 36 0.023515778521945176 37 0.0185265751580247 38 0.019956813455681906 39 0.014003030774736804 40 0.008415123007145869 41 0.007683373180437533 42 0.0071844528440454854 43 0.002893737951073876 44 0.004490283027528429 45 0.0031598287971496353 46 0.0011308860958219745 47 5.321816921515174E-4 48 8.647952497462158E-4 49 3.991362691136381E-4 50 5.321816921515174E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3006492.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.732271910805586 #Duplication Level Percentage of deduplicated Percentage of total 1 76.34761623997986 44.84068956748333 2 13.077498089354266 15.361423473939908 3 4.5581784760108315 8.031365330131488 4 2.109753983926061 4.956425785954031 5 1.1532348540013362 3.3866051511112336 6 0.6722345160288152 2.368911622993991 7 0.4267856547234069 1.7546263784592429 8 0.28749218302426477 1.3508055252489766 9 0.21631539279340994 1.143422502123174 >10 1.054685131925895 11.025544598826006 >50 0.0677955354392626 2.738588522857668 >100 0.02755685078239621 2.58043403120745 >500 6.256008074983402E-4 0.23859539994969814 >1k 2.2749120269791796E-4 0.22256210971374193 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.326135575946984E-5 7 0.0 0.0 0.0 0.0 3.326135575946984E-5 8 0.0 0.0 0.0 0.0 3.326135575946984E-5 9 0.0 0.0 0.0 0.0 6.652271151893968E-5 10 0.0 0.0 0.0 3.326135575946984E-5 6.652271151893968E-5 11 0.0 0.0 0.0 3.326135575946984E-5 6.652271151893968E-5 12 0.0 0.0 0.0 3.326135575946984E-5 6.652271151893968E-5 13 0.0 3.326135575946984E-5 0.0 3.326135575946984E-5 6.652271151893968E-5 14 0.0 3.326135575946984E-5 0.0 3.326135575946984E-5 6.652271151893968E-5 15 0.0 3.326135575946984E-5 0.0 3.326135575946984E-5 9.978406727840952E-5 16 0.0 3.326135575946984E-5 0.0 6.652271151893968E-5 9.978406727840952E-5 17 0.0 3.326135575946984E-5 0.0 6.652271151893968E-5 9.978406727840952E-5 18 0.0 3.326135575946984E-5 0.0 6.652271151893968E-5 9.978406727840952E-5 19 0.0 3.326135575946984E-5 0.0 6.652271151893968E-5 9.978406727840952E-5 20 0.0 3.326135575946984E-5 0.0 6.652271151893968E-5 9.978406727840952E-5 21 0.0 3.326135575946984E-5 0.0 6.652271151893968E-5 9.978406727840952E-5 22 0.0 3.326135575946984E-5 0.0 6.652271151893968E-5 9.978406727840952E-5 23 0.0 3.326135575946984E-5 0.0 9.978406727840952E-5 9.978406727840952E-5 24 0.0 3.326135575946984E-5 0.0 1.9956813455681904E-4 9.978406727840952E-5 25 0.0 3.326135575946984E-5 0.0 1.9956813455681904E-4 9.978406727840952E-5 26 0.0 3.326135575946984E-5 0.0 3.326135575946984E-4 9.978406727840952E-5 27 0.0 3.326135575946984E-5 0.0 4.989203363920476E-4 9.978406727840952E-5 28 0.0 3.326135575946984E-5 0.0 0.001031102028543565 9.978406727840952E-5 29 0.0 3.326135575946984E-5 0.0 0.0019291586340492506 9.978406727840952E-5 30 0.0 3.326135575946984E-5 0.0 0.003525703710503803 9.978406727840952E-5 31 0.0 3.326135575946984E-5 0.0 0.007616850468918594 9.978406727840952E-5 32 0.0 3.326135575946984E-5 0.0 0.01240648569828225 1.3304542303787937E-4 33 0.0 3.326135575946984E-5 0.0 0.01859309786954364 1.3304542303787937E-4 34 0.0 3.326135575946984E-5 0.0 0.02541167580023496 1.3304542303787937E-4 35 0.0 3.326135575946984E-5 0.0 0.034492025922570224 1.663067787973492E-4 36 0.0 3.326135575946984E-5 0.0 0.04440390993889224 1.663067787973492E-4 37 0.0 3.326135575946984E-5 0.0 0.05850672478090745 1.663067787973492E-4 38 0.0 3.326135575946984E-5 0.0 0.07231018742108743 1.663067787973492E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5000 0.0 22.31155 1 GGTATCA 2480 0.0 21.914778 1 TATCAAC 6825 0.0 16.312405 2 TCAACGC 6985 0.0 15.781252 4 CAACGCA 6985 0.0 15.751063 5 ATCAACG 7055 0.0 15.655856 3 AACGCAG 7165 0.0 15.414989 6 TATGCGA 215 2.1645974E-10 15.349748 12 TACCGTA 260 1.8189894E-12 15.23142 7 ACCGTAT 275 0.0 15.200649 8 CGCACGA 215 3.5543053E-9 14.324763 35 GTACCGT 340 0.0 14.236376 6 CCGTATG 295 0.0 14.170097 9 GCGAATC 235 8.221832E-10 14.043387 15 GTATGCG 235 8.221832E-10 14.043387 11 AGCGTAC 295 9.094947E-12 13.424302 23 GTACATG 7690 0.0 13.305108 1 ACGGTTG 285 8.0035534E-11 13.121622 36 GCACCGT 470 0.0 13.10738 6 CGTACAA 290 1.05501385E-10 12.897103 25 >>END_MODULE