FastQCFastQC Report
Mon 6 Feb 2017
SRR2077444_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077444_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807830
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40200.023.31541
GGTATCA18350.023.1409151
CGTGCCG2950.019.3918110
ATCAACG52000.018.2765083
TATCAAC51250.018.2015342
TCAACGC52500.017.9770554
CAACGCA53000.017.8470615
AACGCAG54850.017.4045986
GTGCCGA3550.016.73380511
ACGCAGA63200.014.7581077
GCACCGT5300.014.5259016
GTACATG65500.014.4103741
TACATGG64750.014.2707092
CGCAGAG64800.014.1563298
ACCGTGC6100.014.0645038
CGCACGA3150.013.96943235
AGAGTAC59850.013.9326711
GTACCGA3950.013.921756
CGAATCG3500.013.82899737
TACCGAT3750.013.4920647
Produced by FastQC (version 0.11.5)