FastQCFastQC Report
Mon 6 Feb 2017
SRR2077444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807830
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42400.022.2629931
GGTATCA22050.022.1532021
TTACGCG600.00742517118.32875443
ACCGTCG800.001988764216.5005828
CGCACGA2900.015.92904235
CCGTACA1805.2659743E-915.8911472
TATCAAC61150.015.3284242
GCGCGTT1452.2910517E-615.17051636
CGTACAC2509.094947E-1214.9626593
ATCAACG62400.014.9513743
CAACGCA62350.014.9291375
TCAACGC62450.014.9047024
CGTACAA2551.2732926E-1114.66744425
CGCGTTG1503.2588487E-614.66457137
TCGATCG1057.1755965E-414.66326541
GTACATG66250.014.6136581
AACGCAG64500.014.4284146
TACCGAT3100.014.1940487
GTACCGA3200.013.7504846
CAGACGA4650.013.72140320
Produced by FastQC (version 0.11.5)