FastQCFastQC Report
Mon 6 Feb 2017
SRR2077443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3622187
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47520.13119145974517604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.026.6641671
GTATCAA66050.025.0808661
ATCAACG88550.018.4331573
TCAACGC88700.018.352894
TATCAAC90350.018.2619632
CAACGCA91500.017.8393555
AACGCAG93950.017.3973226
ACGCAGA107550.015.0752817
GTACATG107700.014.7278691
CGCAGAG109950.014.7262098
CGTATTA2102.5447662E-914.6682128
TACATGG108550.014.3286072
AGAGTAC103550.014.29892311
GAGTACT54250.013.91037212
CAGAGTA111500.013.61521810
ACATGGG113500.013.4701773
GCAGAGT116200.013.4047749
GTACTTT57600.013.2161214
AGTACTT58600.013.21567713
CCGTTAA2054.4282024E-712.87780236
Produced by FastQC (version 0.11.5)