FastQCFastQC Report
Mon 6 Feb 2017
SRR2077443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3622187
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52490.14491245206280073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.028.1447351
GTATCAA71500.025.450861
TATCAAC101700.017.71962
ATCAACG102400.017.512523
TCAACGC104400.017.2404984
CAACGCA105300.017.0724835
AACGCAG110000.016.3205386
ATACCGT2550.015.5299236
ACCGTCG2152.1645974E-1015.3493428
TAGCGCG1201.1803314E-414.6691754
ACCGTGC5750.014.539618
TCGATCG1851.2724377E-714.26601241
GTACATG101750.013.9701511
ACGCAGA128850.013.8475667
CGCAGAG130350.013.637588
CTAATAC6550.013.4372283
TACATGG103400.013.3850832
GTATAAT9000.013.2024411
AGAGTAC128700.013.0606211
GAGTACT83750.013.00449112
Produced by FastQC (version 0.11.5)