##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3622187
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.431288610996617	31.0	31.0	34.0	26.0	34.0
2	30.37711332959894	31.0	31.0	34.0	26.0	34.0
3	30.555895374810852	31.0	31.0	34.0	27.0	34.0
4	34.007214978133376	37.0	35.0	37.0	30.0	37.0
5	33.61756253887499	37.0	35.0	37.0	28.0	37.0
6	33.55166202076259	37.0	35.0	37.0	28.0	37.0
7	33.362497297903175	37.0	35.0	37.0	27.0	37.0
8	33.32567700121501	37.0	35.0	37.0	27.0	37.0
9	34.77033598762295	39.0	35.0	39.0	27.0	39.0
10	34.634212148627334	39.0	35.0	39.0	27.0	39.0
11	34.576762602262114	39.0	35.0	39.0	27.0	39.0
12	34.50296933868958	39.0	35.0	39.0	26.0	39.0
13	34.46661174588722	39.0	35.0	39.0	26.0	39.0
14	35.61344762156123	40.0	36.0	41.0	26.0	41.0
15	35.61442852066997	40.0	36.0	41.0	26.0	41.0
16	35.56519472904077	40.0	36.0	41.0	26.0	41.0
17	35.539373036234736	40.0	36.0	41.0	26.0	41.0
18	35.53855612645068	40.0	36.0	41.0	26.0	41.0
19	35.57669993294107	40.0	36.0	41.0	26.0	41.0
20	35.58068454223926	40.0	36.0	41.0	26.0	41.0
21	35.519031458066635	40.0	36.0	41.0	25.0	41.0
22	35.52502479855402	40.0	36.0	41.0	26.0	41.0
23	35.45802936181926	40.0	36.0	41.0	25.0	41.0
24	35.43731038734334	40.0	35.0	41.0	25.0	41.0
25	35.41778847972233	40.0	35.0	41.0	25.0	41.0
26	35.334955097569505	40.0	35.0	41.0	25.0	41.0
27	35.27337848653313	40.0	35.0	41.0	25.0	41.0
28	35.2149367219307	40.0	35.0	41.0	24.0	41.0
29	35.13163759905273	40.0	35.0	41.0	24.0	41.0
30	35.002445483902406	39.0	35.0	41.0	24.0	41.0
31	34.913292162994345	39.0	35.0	41.0	24.0	41.0
32	34.82591870601932	39.0	35.0	41.0	23.0	41.0
33	34.72597687529661	39.0	34.0	41.0	23.0	41.0
34	34.69102948025599	39.0	34.0	41.0	22.0	41.0
35	34.621290121134	39.0	34.0	41.0	22.0	41.0
36	34.5138616531946	39.0	34.0	41.0	20.0	41.0
37	34.41808608997824	39.0	34.0	41.0	19.0	41.0
38	34.334937704762346	39.0	34.0	41.0	18.0	41.0
39	34.23522667382993	39.0	34.0	41.0	18.0	41.0
40	34.14018492143006	39.0	34.0	41.0	16.0	41.0
41	34.07455302556163	39.0	34.0	41.0	16.0	41.0
42	33.95354657283017	39.0	33.0	41.0	15.0	41.0
43	34.04971720123782	39.0	34.0	41.0	15.0	41.0
44	33.97707821269305	39.0	34.0	41.0	9.0	41.0
45	33.896933537666605	39.0	34.0	41.0	9.0	41.0
46	33.73462165260932	39.0	33.0	41.0	2.0	41.0
47	33.578124486670625	39.0	33.0	41.0	2.0	41.0
48	33.41568947158167	39.0	33.0	41.0	2.0	41.0
49	33.253848296623005	39.0	33.0	41.0	2.0	41.0
50	33.10694975162795	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.798482891720969
1101	2	0.9081320779581148
1101	3	0.8221380444152615
1101	4	0.7052110537012837
1101	5	0.8816558993534116
1101	6	0.8810267903374438
1101	7	0.8712111885690774
1101	8	0.8601353859403957
1101	9	0.9305760788593815
1101	10	1.0009380594837936
1101	11	1.0122338900680319
1101	12	1.0712385936302624
1101	13	1.173653057094839
1101	14	1.247063492458345
1101	15	1.2353020829294934
1101	16	1.2980970693973646
1101	17	1.2283647035330745
1101	18	1.2596290328984523
1101	19	1.2372625672690134
1101	20	1.2548594195771159
1101	21	1.2627599550299564
1101	22	1.1873890993390077
1101	23	1.3343930354801259
1101	24	1.2908785026514806
1101	25	1.3036045769940046
1101	26	1.2739978136435397
1101	27	1.1815877770472838
1101	28	1.2555466025734248
1101	29	1.2885100730046375
1101	30	1.3805638320460645
1101	31	1.3864386345680515
1101	32	1.3472215972592991
1101	33	1.3841612515664252
1101	34	1.4680538427827798
1101	35	1.3770895082751764
1101	36	1.3595700153606387
1101	37	1.4666350192438244
1101	38	1.4511477366882986
1101	39	1.464684553694731
1101	40	1.5173012405943282
1101	41	1.5252854681242098
1101	42	1.5940976871974044
1101	43	1.4423873044942965
1101	44	1.579785338845376
1101	45	1.5834634959919853
1101	46	1.5374215119597068
1101	47	1.5381058467088948
1101	48	1.5975522406247222
1101	49	1.6937276948460323
1101	50	1.8121486134521732
1102	1	0.7120519110371148
1102	2	0.8575592365806735
1102	3	0.7349763342650455
1102	4	0.7968123430037224
1102	5	0.8419265780148919
1102	6	0.7872964678714851
1102	7	0.8061804764119245
1102	8	0.8554637697745235
1102	9	0.984501761520697
1102	10	1.0197681438311221
1102	11	1.1172002306665547
1102	12	1.1607131888519788
1102	13	1.1684280139387653
1102	14	1.2265118431710604
1102	15	1.255117388951696
1102	16	1.1617622250165454
1102	17	1.24022291769014
1102	18	1.1486376828266387
1102	19	1.2090246919047019
1102	20	1.190180973289479
1102	21	1.2808649237096432
1102	22	1.1586777936162065
1102	23	1.206192585440597
1102	24	1.2272591930551613
1102	25	1.185011207825923
1102	26	1.2207125718525518
1102	27	1.135853562347549
1102	28	1.1868570971023118
1102	29	1.2201428931432048
1102	30	1.298798853729366
1102	31	1.2990252391086798
1102	32	1.3188658321219222
1102	33	1.339287351520106
1102	34	1.2822885650634746
1102	35	1.326235509145313
1102	36	1.3078828349378284
1102	37	1.3025801724303534
1102	38	1.3040072654098935
1102	39	1.2194261084362807
1102	40	1.2285737484185546
1102	41	1.2433441229644728
1102	42	1.2597922310528702
1102	43	1.2300477070972278
1102	44	1.2676319324390946
1102	45	1.2370027621749315
1102	46	1.3400174260498758
1102	47	1.446612155215206
1102	48	1.3917012589116737
1102	49	1.357678686383231
1102	50	1.4308856023041088
1103	1	0.44323955418242633
1103	2	0.5993439420276587
1103	3	0.5099839121780789
1103	4	0.4943413926239941
1103	5	0.4705600603602491
1103	6	0.5549620171982994
1103	7	0.5706818268468083
1103	8	0.5593154237765319
1103	9	0.6982598470716539
1103	10	0.7131184339518271
1103	11	0.655254585938863
1103	12	0.7719405733054359
1103	13	0.7595771116485679
1103	14	0.8548173188229669
1103	15	0.7806067132850885
1103	16	0.8686173646439741
1103	17	0.8904199434290092
1103	18	0.8433499231123562
1103	19	0.8257821290237572
1103	20	0.8500142863816649
1103	21	0.9521698167312493
1103	22	0.895540753621276
1103	23	0.9848244728122779
1103	24	0.8758477271828085
1103	25	0.9086967528298544
1103	26	1.013726830345
1103	27	1.0114842385405751
1103	28	1.0149634227678987
1103	29	1.0191953071251305
1103	30	1.0934550052996386
1103	31	1.03232618660887
1103	32	0.973541586848448
1103	33	0.9993590523814362
1103	34	1.022552180595575
1103	35	0.9788195596088514
1103	36	1.0458730765598858
1103	37	1.0481448939586784
1103	38	1.0000479828705409
1103	39	1.0218665898122126
1103	40	1.0060513898201293
1103	41	0.9872471394101581
1103	42	1.0837263058558477
1103	43	1.0222136970802183
1103	44	1.0663549092579032
1103	45	0.9683627053910087
1103	46	1.071227312998424
1103	47	1.0673937895868733
1103	48	0.98161750634344
1103	49	1.0349595094640307
1103	50	1.143926879507525
1104	1	0.5254869727912137
1104	2	0.6820043804500706
1104	3	0.6083007000433334
1104	4	0.600686974267731
1104	5	0.6059343941468498
1104	6	0.6400223773818823
1104	7	0.6642011692347438
1104	8	0.6701289108544373
1104	9	0.7755809513484522
1104	10	0.7928724070432551
1104	11	0.8085888520769586
1104	12	0.7886017036159672
1104	13	0.7964607124195311
1104	14	0.9140707524932807
1104	15	0.9271160390041757
1104	16	0.9272983606375647
1104	17	0.9392913017977946
1104	18	0.8552715780776055
1104	19	0.8178248942991502
1104	20	0.8839843667036646
1104	21	0.8276032633704773
1104	22	0.8948469540134596
1104	23	0.878880268299767
1104	24	0.8983324229636906
1104	25	0.8893396985911508
1104	26	0.8784514767358829
1104	27	0.8719700449290926
1104	28	0.86988065580762
1104	29	0.9006261601222576
1104	30	1.01931541169251
1104	31	0.9416264933709613
1104	32	0.912865452143933
1104	33	0.9539362217833016
1104	34	0.9752295075107682
1104	35	1.0526184570503077
1104	36	0.9954124051587172
1104	37	0.8737944044551327
1104	38	0.9559445635989192
1104	39	0.9876128103368487
1104	40	0.976941555951818
1104	41	0.9419200915961241
1104	42	1.0315287200129575
1104	43	1.030692141679019
1104	44	1.0109485653931216
1104	45	1.0258260158233554
1104	46	0.9703465284359396
1104	47	1.0683380632603914
1104	48	1.0467976561746823
1104	49	1.0169198160495725
1104	50	1.0328924247440767
1105	1	0.3788618322625119
1105	2	0.5468302963311302
1105	3	0.40473501684963153
1105	4	0.4249552962097525
1105	5	0.4737357556609112
1105	6	0.599513197035229
1105	7	0.562166920581717
1105	8	0.5700315120966835
1105	9	0.5754266062302378
1105	10	0.5640545274783122
1105	11	0.6095506052709254
1105	12	0.5530069758051468
1105	13	0.635590442202961
1105	14	0.6709885887411602
1105	15	0.7053547042016319
1105	16	0.6103864211176102
1105	17	0.6136678075353643
1105	18	0.6089892180309917
1105	19	0.6025930663393453
1105	20	0.6941423889413869
1105	21	0.7237587274108463
1105	22	0.607408345374516
1105	23	0.6575016492391441
1105	24	0.6804535316574061
1105	25	0.6424162922284822
1105	26	0.5952894247725453
1105	27	0.652540898000403
1105	28	0.6886603121099029
1105	29	0.6406794633854105
1105	30	0.7361032790489261
1105	31	0.6892119377478636
1105	32	0.6609507166794728
1105	33	0.7729534891391481
1105	34	0.6766790778307765
1105	35	0.6696603069435341
1105	36	0.7519412996018389
1105	37	0.7799476325564356
1105	38	0.8386301278292265
1105	39	0.738311694395037
1105	40	0.7315140377339517
1105	41	0.7866604225885965
1105	42	0.7727002649869732
1105	43	0.8285509000074853
1105	44	0.8328665996502167
1105	45	0.8390336861150232
1105	46	0.8537396864079625
1105	47	0.8726353190432476
1105	48	0.8931094386209466
1105	49	0.8579496517509213
1105	50	0.9135613229949655
1106	1	0.3770291239822434
1106	2	0.4925358477657973
1106	3	0.4411455887498974
1106	4	0.4303058409254632
1106	5	0.5264607182069625
1106	6	0.4289368547281214
1106	7	0.3989655922807458
1106	8	0.4386261154814193
1106	9	0.5318490164784961
1106	10	0.5282131836980639
1106	11	0.4707193600167088
1106	12	0.479168540511111
1106	13	0.4700717466356039
1106	14	0.49012748199934464
1106	15	0.4888562575855815
1106	16	0.5416462204246102
1106	17	0.5003354536336957
1106	18	0.5548456764646303
1106	19	0.42389981715196967
1106	20	0.4691505663973743
1106	21	0.5151666630962524
1106	22	0.48140366273299406
1106	23	0.557731296688992
1106	24	0.5109942205225764
1106	25	0.5526358100127524
1106	26	0.556286153524006
1106	27	0.5276541115183733
1106	28	0.5824352254049145
1106	29	0.5665310299837003
1106	30	0.6973617662368667
1106	31	0.5842957097627846
1106	32	0.6061030755262067
1106	33	0.6356910645294391
1106	34	0.6284984253855654
1106	35	0.5762150070045635
1106	36	0.6578356151986782
1106	37	0.6675470694896859
1106	38	0.6719236052135358
1106	39	0.6087940325539378
1106	40	0.5974351652727208
1106	41	0.6383990202606924
1106	42	0.7097017828399146
1106	43	0.657443588629917
1106	44	0.5029495000241795
1106	45	0.4548782955546642
1106	46	0.4741027228697021
1106	47	0.48601351726690467
1106	48	0.4746311560543717
1106	49	0.5877518677682971
1106	50	0.6314543951394214
1107	1	0.298004518351938
1107	2	0.46900185459292487
1107	3	0.29969088825481904
1107	4	0.36614215408717854
1107	5	0.2619966628435648
1107	6	0.32465785744116005
1107	7	0.2954668025961169
1107	8	0.31019065291864933
1107	9	0.38289233484248797
1107	10	0.34260231632807603
1107	11	0.48319789290586357
1107	12	0.42364414620467983
1107	13	0.4432613050276757
1107	14	0.46957173181719014
1107	15	0.44499044521420217
1107	16	0.40189895295921474
1107	17	0.4348331651007413
1107	18	0.3913622785331512
1107	19	0.37160515655400417
1107	20	0.3666367928827441
1107	21	0.42045020943331934
1107	22	0.33534769197524383
1107	23	0.4354466390365914
1107	24	0.4004932386337501
1107	25	0.4065094739764632
1107	26	0.3535965580272773
1107	27	0.37704896034997404
1107	28	0.3862014161786078
1107	29	0.4694874076156452
1107	30	0.4894898657052309
1107	31	0.6013780213064877
1107	32	0.48678301130355806
1107	33	0.5540232653944202
1107	34	0.477505157583586
1107	35	0.49592236041723936
1107	36	0.3717077193987137
1107	37	0.44683086692655394
1107	38	0.4444931217109058
1107	39	0.34835760755051837
1107	40	0.3856848348328157
1107	41	0.38633622628611874
1107	42	0.3206342732840781
1107	43	0.3038667618967992
1107	44	0.4238102250510707
1107	45	0.3072895344793878
1107	46	0.3976395111078048
1107	47	0.32399928771360464
1107	48	0.297623942505723
1107	49	0.32204648419250503
1107	50	0.3697717880224616
1108	1	0.16485886558758267
1108	2	0.3275690149980477
1108	3	0.27414842826085106
1108	4	0.19198293073532824
1108	5	0.16237032988057365
1108	6	0.16974068429477995
1108	7	0.16815624656282324
1108	8	0.09289907113853957
1108	9	0.15176875772996823
1108	10	0.0971328343302531
1108	11	0.12082373274026992
1108	12	0.10245567728180305
1108	13	0.14945802615823567
1108	14	0.19163430519395064
1108	15	0.13537387963050662
1108	16	0.1993776725506251
1108	17	0.0899796872454317
1108	18	0.14594116715066008
1108	19	0.16816609292909135
1108	20	0.11214617499121715
1108	21	0.24943656043315343
1108	22	0.09911909496413074
1108	23	0.18422970179619114
1108	24	0.21091405040238698
1108	25	0.14587530390892312
1108	26	0.13617680541054256
1108	27	0.1928273905340845
1108	28	0.1859663007833916
1108	29	0.13322402183843707
1108	30	0.327923588394583
1108	31	0.2830126667226125
1108	32	0.217379626218829
1108	33	0.24263760667199108
1108	34	0.18252552729191507
1108	35	0.12566843662938965
1108	36	0.30739225831396766
1108	37	0.24163513356977973
1108	38	0.19164061875421368
1108	39	0.23796063258878064
1108	40	0.3326684784334759
1108	41	0.26547896861733733
1108	42	0.3247262208221926
1108	43	0.2429921650242477
1108	44	0.24796629092146105
1108	45	0.33392712585636275
1108	46	0.37769839418415074
1108	47	0.32978017321467945
1108	48	0.3812749681676735
1108	49	0.3259319450278113
1108	50	0.41857861014434405
1109	1	0.14324265417240412
1109	2	0.34130815740745035
1109	3	0.2018139758271822
1109	4	0.2083045124055971
1109	5	0.2069644906887831
1109	6	0.18008036367707092
1109	7	0.18248866875282488
1109	8	0.11950669449999651
1109	9	0.04762509392948999
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2211	3	-0.14814449276994068
2211	4	-0.05174517549347968
2211	5	-0.12745179190474687
2211	6	-0.09168611535191928
2211	7	-0.1138994466422929
2211	8	-0.12389117523461834
2211	9	-0.2853692192363795
2211	10	-0.15958148135002403
2211	11	-0.16773378089141033
2211	12	-0.10121897921872147
2211	13	-0.055507724371238965
2211	14	-0.18104197886361817
2211	15	-0.1701306521181607
2211	16	-0.2165558050321721
2211	17	-0.18577784959526156
2211	18	-0.22120055448728948
2211	19	-0.10908096642270237
2211	20	-0.11056323334913287
2211	21	-0.15866957105621537
2211	22	-0.17333152635600158
2211	23	-0.16717676131789716
2211	24	-0.13596034067271034
2211	25	-0.09563870633378002
2211	26	-0.22926295235826188
2211	27	-0.1242927990111582
2211	28	-0.12206492187514328
2211	29	-0.12754506576966662
2211	30	-0.1296738341232171
2211	31	-0.19424380435417987
2211	32	-0.1410701702818784
2211	33	-0.05145074287824514
2211	34	-0.08969180274275601
2211	35	-0.07697444104002216
2211	36	-0.1848343990604704
2211	37	-0.010612955189436946
2211	38	0.027180887648057706
2211	39	-0.007139934741744014
2211	40	0.04928923060338519
2211	41	-0.032854573985211744
2211	42	-0.12529670331883835
2211	43	-0.23888384307512922
2211	44	-0.2018257441414022
2211	45	-0.22276088565402574
2211	46	-0.24633777788373834
2211	47	-0.17749193961933685
2211	48	-0.1656115448150075
2211	49	-0.20603276457596564
2211	50	-0.2180580323188508
2212	1	-1.0422294027547636
2212	2	-2.401470943472866
2212	3	-1.3886286480718617
2212	4	-0.894810828041031
2212	5	-0.7837197445168584
2212	6	-0.605777643778822
2212	7	-0.6661391950178697
2212	8	-0.7960232304332777
2212	9	-0.8209352930820941
2212	10	-0.7721618394225587
2212	11	-0.803016371418785
2212	12	-0.7426287417424504
2212	13	-2.2401639502705564
2212	14	-1.6518511731229921
2212	15	-1.2466244731906073
2212	16	-1.0550904440052022
2212	17	-0.9183843260116547
2212	18	-0.8427385716196909
2212	19	-0.7838550646013047
2212	20	-0.9033845268388703
2212	21	-0.8761654763685911
2212	22	-0.8584474615513145
2212	23	-0.805383158059513
2212	24	-0.7895360250748737
2212	25	-0.8362639574981898
2212	26	-0.8649503747670195
2212	27	-0.9487153726107067
2212	28	-0.9432752844205012
2212	29	-0.9589938766498491
2212	30	-0.918286063578023
2212	31	-0.9148143139967218
2212	32	-0.9243470173200663
2212	33	-0.8874603168293405
2212	34	-0.9016031827882287
2212	35	-0.9599921675161767
2212	36	-0.8625751831286479
2212	37	-0.7917065197428741
2212	38	-0.8704784268733476
2212	39	-0.7839414917055123
2212	40	-0.8441321885810353
2212	41	-0.7589233537190196
2212	42	-0.8274658945812305
2212	43	-0.8360819363405412
2212	44	-0.824472848208508
2212	45	-0.9289386379155218
2212	46	-0.7833980905803273
2212	47	-0.8357345303572927
2212	48	-0.8225050020131661
2212	49	-0.6999861182226184
2212	50	-0.7838951675378425
2213	1	-1.0679819084262014
2213	2	-2.198384332397737
2213	3	-1.342130587218115
2213	4	-1.0095923253254853
2213	5	-0.925559585698025
2213	6	-0.9516422728726326
2213	7	-1.0580400656385507
2213	8	-1.0032208686898585
2213	9	-1.1506672399057507
2213	10	-1.124043668115732
2213	11	-1.0772446877177018
2213	12	-1.2096203832147552
2213	13	-1.1935058456661523
2213	14	-1.2598004664125995
2213	15	-1.2713818865817004
2213	16	-1.307145493611344
2213	17	-1.347628594100712
2213	18	-1.2237478061502927
2213	19	-1.2515462711035283
2213	20	-1.4016497812601827
2213	21	-1.2865422132948936
2213	22	-1.3997272424021148
2213	23	-1.3212187045993318
2213	24	-1.2935752893066734
2213	25	-1.354056157979123
2213	26	-1.3722012784543338
2213	27	-1.3933499476286642
2213	28	-1.3010058553751875
2213	29	-1.3306806709679577
2213	30	-1.337362511534728
2213	31	-1.4403042043911896
2213	32	-1.339900213771763
2213	33	-1.3301449192842512
2213	34	-1.4684765391228254
2213	35	-1.372399610323889
2213	36	-1.3420449167953024
2213	37	-1.3757718660950005
2213	38	-1.4888904488996246
2213	39	-1.421332957977235
2213	40	-1.4827822903792551
2213	41	-1.5203070299988255
2213	42	-1.6065984042491195
2213	43	-1.5633141816609424
2213	44	-1.515660234261631
2213	45	-1.485608663830611
2213	46	-1.5485219156940175
2213	47	-1.5463782749480046
2213	48	-1.5768320913512355
2213	49	-1.6739995168602348
2213	50	-1.6536772741887198
2214	1	-0.9622616437028277
2214	2	-1.9981159574128498
2214	3	-1.094732511701853
2214	4	-0.6900937071971356
2214	5	-0.6977945295255239
2214	6	-0.63455157983622
2214	7	-0.6449065449617635
2214	8	-0.6643870389086004
2214	9	-0.6755160156395235
2214	10	-0.6928806609969271
2214	11	-0.5701856623188633
2214	12	-0.7153206190427852
2214	13	-0.6560500405228851
2214	14	-0.7735761940782453
2214	15	-0.745979629422898
2214	16	-0.7347185654943118
2214	17	-0.672829890605307
2214	18	-0.7853508871806056
2214	19	-0.7721821396797779
2214	20	-0.7665804067845627
2214	21	-0.7953674453064465
2214	22	-0.7081505531052414
2214	23	-0.7022050544186413
2214	24	-0.8062847225499965
2214	25	-0.7591483824932652
2214	26	-0.7959545102509367
2214	27	-0.7878868221683959
2214	28	-0.825453931074648
2214	29	-0.702685099307871
2214	30	-0.695142088656354
2214	31	-0.7606104510861158
2214	32	-0.6755813244472861
2214	33	-0.6765434430081143
2214	34	-0.8333212480138172
2214	35	-0.8897288792569924
2214	36	-0.8354160852004568
2214	37	-0.8579269141704557
2214	38	-0.9517555945915177
2214	39	-1.018464062973834
2214	40	-0.93596853345921
2214	41	-0.9429679542346463
2214	42	-0.8597074628055523
2214	43	-0.8327042895849388
2214	44	-0.7681462598326618
2214	45	-0.9030051028395718
2214	46	-0.8597543400330352
2214	47	-0.8326156149152979
2214	48	-0.8338164969021662
2214	49	-0.8471113354961446
2214	50	-0.8937522848443393
2215	1	-0.33126074805450045
2215	2	-0.17124884632457693
2215	3	-0.4176730101910948
2215	4	-0.37858205697142466
2215	5	-0.45229345149858347
2215	6	-0.5694594100883918
2215	7	-0.571517819070479
2215	8	-0.6381086201622423
2215	9	-0.761017595985308
2215	10	-0.7035575863355277
2215	11	-0.7266659901994288
2215	12	-0.681886288130336
2215	13	-0.7441608562080404
2215	14	-0.9172247524577131
2215	15	-0.9010747209152044
2215	16	-0.8527656834629767
2215	17	-0.947955395429986
2215	18	-0.795007218137755
2215	19	-0.8109753255633265
2215	20	-0.8557333612604197
2215	21	-0.7823194252376524
2215	22	-0.8294982860918978
2215	23	-0.818102570313691
2215	24	-0.8671369640787248
2215	25	-0.9101446213232975
2215	26	-0.9431162474722825
2215	27	-0.9202158096756321
2215	28	-0.9764568521278676
2215	29	-1.0520481451850188
2215	30	-0.8625587388670866
2215	31	-0.947578134241823
2215	32	-0.8876551570225715
2215	33	-0.8594642335473708
2215	34	-0.7724229797926085
2215	35	-0.8821059603374763
2215	36	-0.8264079874980581
2215	37	-0.7517373436461554
2215	38	-0.8369831692233092
2215	39	-0.796688333591888
2215	40	-0.7858078029493569
2215	41	-0.9186105018391473
2215	42	-0.9115827135329369
2215	43	-0.8137393336979812
2215	44	-0.8528082418768435
2215	45	-0.7849843681314326
2215	46	-0.6518480257238366
2215	47	-0.568820222745984
2215	48	-0.6390238151808347
2215	49	-0.5397685860654562
2215	50	-0.6278946203458631
2216	1	-1.0751822586579927
2216	2	-0.6853321832132941
2216	3	-0.7989742508855109
2216	4	-2.1966580360613754
2216	5	-1.369041838473791
2216	6	-1.1454836806319264
2216	7	-1.1376133866241105
2216	8	-1.1928755822496768
2216	9	-1.0984110622612846
2216	10	-1.1979129831975186
2216	11	-1.3627278013191741
2216	12	-1.1751282831490144
2216	13	-1.4338267912238933
2216	14	-1.4383265251161461
2216	15	-1.5141636190317342
2216	16	-1.46030023256467
2216	17	-1.3708291520201854
2216	18	-1.3726101060983993
2216	19	-1.4134259070145418
2216	20	-1.5858335984745935
2216	21	-1.5012882938283454
2216	22	-1.5308794578934055
2216	23	-1.4887725551487492
2216	24	-1.690056259923118
2216	25	-1.6013154608691877
2216	26	-1.7269051348343325
2216	27	-1.8189283876047924
2216	28	-1.707214723922796
2216	29	-1.7108645142851984
2216	30	-1.7500868716821856
2216	31	-1.628792916197014
2216	32	-1.6582095566246835
2216	33	-1.6336742195611933
2216	34	-1.728220112934089
2216	35	-1.73667514491396
2216	36	-1.8432509807080706
2216	37	-1.83425404935781
2216	38	-1.9403228554976266
2216	39	-2.018439261440143
2216	40	-1.9793369730980288
2216	41	-2.0608177033580333
2216	42	-2.0350217682050378
2216	43	-1.8823673698512309
2216	44	-1.8746557352131035
2216	45	-1.8511262672055935
2216	46	-1.7551676376824972
2216	47	-1.8346375656378342
2216	48	-1.8304781308294835
2216	49	-1.8145282068418283
2216	50	-1.7795784245004072
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99506.0
3	16035.0
4	27382.0
5	15600.0
6	11416.0
7	8960.0
8	7715.0
9	6621.0
10	6439.0
11	6343.0
12	6468.0
13	6935.0
14	7365.0
15	7913.0
16	8724.0
17	9400.0
18	10205.0
19	11304.0
20	13221.0
21	16396.0
22	20487.0
23	25101.0
24	29577.0
25	32857.0
26	35972.0
27	40647.0
28	47870.0
29	54322.0
30	62658.0
31	74055.0
32	91486.0
33	112628.0
34	142191.0
35	181021.0
36	235329.0
37	317554.0
38	503821.0
39	1310663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67036432138257	18.172801736218013	14.15705207411508	25.999781868284337
2	14.629316996878938	20.148916462944122	38.29657124951514	26.9251952906618
3	17.36996359648808	24.86360416302015	29.772805201732215	27.993627038759556
4	12.034540269695047	16.539534760446383	36.9628758636931	34.46304910616547
5	13.581233337411666	37.84461332852403	34.275308422732124	14.298844911332175
6	32.17763147902425	37.122891413217424	17.21460121699821	13.48487589076012
7	27.989433010189202	32.150834224409905	21.701709541441687	18.15802322395921
8	26.849493388591327	34.20124065395011	19.78311199574961	19.166153961708947
9	26.746247635354386	14.980240537965509	18.888029715137876	39.38548211154223
10	14.361506020552811	28.690093076164047	33.723933054092875	23.224467849190265
11	34.81073750989086	22.86127379158824	23.664474220226943	18.66351447829395
12	23.59586341674662	24.56748102473344	30.348495318393955	21.488160240125982
13	27.99839374599369	21.724198785532778	26.245875619709974	24.031531848763564
14	22.48367839914358	20.473272567281896	27.33914405708127	29.703904976493252
15	23.648299131662462	28.542926795453084	24.04130327132448	23.767470801559977
16	23.56578844521632	27.737706294486596	25.228876988509956	23.467628271787124
17	22.27906446180646	27.49302798720746	26.816362278959506	23.411545272026572
18	22.422498455990482	26.831405208863885	27.919945730413176	22.826150604732458
19	23.33296780910023	26.426076222574977	28.098777721951702	22.142178246373092
20	23.731202955600804	25.770608388417564	27.819266645969805	22.67892201001183
21	23.727584211551182	25.751672077989333	27.816721194201342	22.704022516258135
22	23.463446902477656	25.493135720904668	27.81189624242692	23.231521134190753
23	23.125106854042972	25.67753403931345	27.666379512969286	23.530979593674296
24	22.977801861757953	25.840066904855018	27.74134799583723	23.440783237549798
25	23.19647763390327	25.66268074994449	27.765837335268845	23.375004280883395
26	23.072786524109485	25.933366826592835	27.73821896692536	23.255627682372317
27	23.008048592607977	26.157304537631955	27.720042324090844	23.114604545669224
28	23.165496159255294	25.822835224152996	27.705838616912093	23.305829999679617
29	23.1721904438898	25.95245006477956	27.700213851253096	23.175145640077545
30	23.301998097681093	25.9632284627562	27.558105237342456	23.176668202220256
31	23.26814158216668	25.640005214161754	27.690512987350136	23.40134021632143
32	23.155844435594915	26.031708070125823	27.499852252146763	23.3125952421325
33	23.179210640115716	25.93379088364058	27.46635613481736	23.420642341426344
34	23.326346228136764	25.844106462828385	27.67923636858669	23.15031094044816
35	23.452045020070926	25.900319958513364	27.428289612297096	23.21934540911861
36	23.422090484687722	25.921716314007348	27.23274962707195	23.423443574232977
37	23.37557983202095	25.704351456555706	27.570196295867788	23.349872415555556
38	23.430262772037622	25.72245604404404	27.438308132673345	23.408973051244995
39	23.426956705656004	26.080453403591818	27.22978379611196	23.26280609464021
40	23.443103497307078	25.8751412586926	27.431423443103498	23.250331800896824
41	23.302819924873788	25.874891804174876	27.38238578869407	23.439902482257267
42	23.567720807954416	25.928860717345444	27.19080977940809	23.31260869529205
43	23.280494565565498	25.73501803379708	27.312643977727763	23.671843422909657
44	23.36125962338851	26.074264881482375	26.94615043753028	23.618325057598835
45	23.504525166085646	26.030423980495172	26.851361801529894	23.61368905188929
46	23.600435653459485	25.827596631277878	26.7776966410787	23.794271074183943
47	23.43257849220427	26.137445043378634	26.75179967837004	23.678176786047057
48	23.532167675594764	26.270036036498656	26.63432133469495	23.56347495321163
49	23.610940480334914	26.07625074506216	26.565872881559482	23.746935893043446
50	23.671627220764535	26.196739197653564	26.522040894754976	23.609592686826925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	804.0
1	584.0
2	364.0
3	607.0
4	850.0
5	971.5
6	1093.0
7	1045.5
8	998.0
9	1346.5
10	1695.0
11	2448.0
12	3201.0
13	6067.5
14	8934.0
15	12397.0
16	15860.0
17	17900.5
18	19941.0
19	21057.5
20	22174.0
21	24443.0
22	26712.0
23	30966.5
24	35221.0
25	41421.0
26	47621.0
27	55458.0
28	63295.0
29	70868.5
30	78442.0
31	87722.0
32	97002.0
33	107950.0
34	118898.0
35	129511.0
36	140124.0
37	150533.5
38	160943.0
39	170775.5
40	180608.0
41	192086.5
42	203565.0
43	212236.5
44	220908.0
45	229995.5
46	239083.0
47	245959.0
48	252835.0
49	253749.0
50	254663.0
51	255020.0
52	255377.0
53	250037.5
54	244698.0
55	234000.5
56	223303.0
57	207684.5
58	192066.0
59	175453.0
60	158840.0
61	139719.5
62	120599.0
63	103189.5
64	85780.0
65	71407.5
66	57035.0
67	46394.5
68	35754.0
69	29150.0
70	22546.0
71	17609.0
72	12672.0
73	10413.5
74	8155.0
75	6197.5
76	4240.0
77	3278.5
78	2317.0
79	1841.5
80	1366.0
81	1058.5
82	751.0
83	572.5
84	394.0
85	327.0
86	260.0
87	185.0
88	110.0
89	76.5
90	43.0
91	32.0
92	21.0
93	15.0
94	9.0
95	9.5
96	10.0
97	5.5
98	1.0
99	3.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014411183078068582
2	6.073678691906299E-4
3	2.484686737598031E-4
4	0.0026503325201045667
5	0.03936848097571992
6	0.027110693070236294
7	0.030340785829113738
8	0.02404624609386539
9	0.03138987578498846
10	0.032411358110445425
11	0.03925805045404889
12	0.03923044282363114
13	0.03699422475979291
14	0.04210163638707775
15	0.03754637736814803
16	0.04345441027754779
17	0.04527651388511968
18	0.048120099818148535
19	0.05013545683864472
20	0.04613235042807012
21	0.046712110666842985
22	0.045055652841777635
23	0.04400656288590291
24	0.04204642112624224
25	0.03972738017115074
26	0.04301268819086369
27	0.04290225766919267
28	0.042377712691255315
29	0.039920633584075035
30	0.03707704765104618
31	0.0353101593043098
32	0.03183159787167256
33	0.0241014613547009
34	0.02081615333498795
35	0.021533951725849605
36	0.023328447703003737
37	0.01833146659738992
38	0.019905101531202004
39	0.014162714404308777
40	0.00789578229947819
41	0.007785351777807164
42	0.006901907604438976
43	0.0027331554113578346
44	0.004196359823498897
45	0.0030368393459531493
46	0.0011595204775457478
47	3.3129156501307083E-4
48	7.177983908616534E-4
49	1.6564578250653542E-4
50	3.0368393459531494E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3622187.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.90192807609198
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.85214828218122	36.065109558961915
2	14.16246013365706	14.417930542078631
3	5.807078625366182	8.867744955618011
4	2.9653076803788236	6.03759513080504
5	1.715882062773672	4.36708526731809
6	1.0951349091185023	3.3446687026540514
7	0.7312562312913778	2.6055646463271454
8	0.4994867508425886	2.033987093308025
9	0.3762999185553723	1.7238952250410344
>10	1.6839383357869016	14.76368481556487
>50	0.0811482600639431	2.8195110436843853
>100	0.029367466379517395	2.537634590691275
>500	3.2756240473830097E-4	0.10351500556445993
>1k	1.0918746676900875E-4	0.16620758141347572
>5k	5.4593733384504375E-5	0.14586584096962615
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5249	0.14491245206280073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.7607630417755903E-5	0.0	0.0	0.0	0.0
3	2.7607630417755903E-5	0.0	0.0	0.0	0.0
4	2.7607630417755903E-5	0.0	0.0	0.0	0.0
5	5.5215260835511805E-5	0.0	0.0	0.0	0.0
6	5.5215260835511805E-5	0.0	0.0	0.0	0.0
7	8.282289125326771E-5	0.0	0.0	0.0	0.0
8	8.282289125326771E-5	0.0	0.0	0.0	0.0
9	8.282289125326771E-5	0.0	0.0	0.0	0.0
10	8.282289125326771E-5	0.0	0.0	0.0	0.0
11	1.1043052167102361E-4	0.0	0.0	0.0	0.0
12	1.3803815208877953E-4	0.0	0.0	0.0	0.0
13	3.0368393459531494E-4	0.0	0.0	0.0	0.0
14	3.588991954308267E-4	2.7607630417755903E-5	0.0	0.0	8.282289125326771E-5
15	3.588991954308267E-4	2.7607630417755903E-5	0.0	2.7607630417755903E-5	1.1043052167102361E-4
16	3.588991954308267E-4	2.7607630417755903E-5	0.0	2.7607630417755903E-5	1.1043052167102361E-4
17	3.8650682584858266E-4	2.7607630417755903E-5	0.0	2.7607630417755903E-5	1.1043052167102361E-4
18	4.6932971710185033E-4	2.7607630417755903E-5	0.0	2.7607630417755903E-5	1.3803815208877953E-4
19	7.454060212794093E-4	2.7607630417755903E-5	0.0	2.7607630417755903E-5	1.3803815208877953E-4
20	7.730136516971653E-4	2.7607630417755903E-5	0.0	2.7607630417755903E-5	1.6564578250653542E-4
21	7.730136516971653E-4	2.7607630417755903E-5	0.0	2.7607630417755903E-5	1.6564578250653542E-4
22	8.282289125326771E-4	2.7607630417755903E-5	0.0	5.5215260835511805E-5	1.9325341292429133E-4
23	8.282289125326771E-4	2.7607630417755903E-5	0.0	1.1043052167102361E-4	2.7607630417755905E-4
24	9.386594342037007E-4	2.7607630417755903E-5	0.0	1.9325341292429133E-4	3.0368393459531494E-4
25	9.386594342037007E-4	2.7607630417755903E-5	0.0	2.484686737598031E-4	3.0368393459531494E-4
26	9.386594342037007E-4	2.7607630417755903E-5	0.0	3.0368393459531494E-4	3.0368393459531494E-4
27	9.386594342037007E-4	2.7607630417755903E-5	0.0	4.4172208668409444E-4	3.0368393459531494E-4
28	0.0010214823254569684	2.7607630417755903E-5	0.0	7.177983908616534E-4	3.0368393459531494E-4
29	0.0012699509992167715	2.7607630417755903E-5	0.0	0.0014632044121410629	3.0368393459531494E-4
30	0.0012975586296345273	5.5215260835511805E-5	0.0	0.0022086104334204724	3.0368393459531494E-4
31	0.0012975586296345273	5.5215260835511805E-5	0.0	0.004141144562663386	3.0368393459531494E-4
32	0.0012975586296345273	5.5215260835511805E-5	0.0	0.005990855800653031	3.0368393459531494E-4
33	0.0012975586296345273	5.5215260835511805E-5	0.0	0.008447934907833306	3.0368393459531494E-4
34	0.0013251662600522833	5.5215260835511805E-5	0.0	0.011512381884204212	3.0368393459531494E-4
35	0.0014632044121410629	5.5215260835511805E-5	0.0	0.015073766208094723	3.0368393459531494E-4
36	0.0015736349338120864	5.5215260835511805E-5	0.0	0.019352948922846887	3.0368393459531494E-4
37	0.0015736349338120864	5.5215260835511805E-5	0.0	0.024432752919713974	3.0368393459531494E-4
38	0.0017944959771541338	5.5215260835511805E-5	0.0	0.03094815369830437	3.3129156501307083E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3010	0.0	28.144735	1
GTATCAA	7150	0.0	25.45086	1
TATCAAC	10170	0.0	17.7196	2
ATCAACG	10240	0.0	17.51252	3
TCAACGC	10440	0.0	17.240498	4
CAACGCA	10530	0.0	17.072483	5
AACGCAG	11000	0.0	16.320538	6
ATACCGT	255	0.0	15.529923	6
ACCGTCG	215	2.1645974E-10	15.349342	8
TAGCGCG	120	1.1803314E-4	14.669175	4
ACCGTGC	575	0.0	14.53961	8
TCGATCG	185	1.2724377E-7	14.266012	41
GTACATG	10175	0.0	13.970151	1
ACGCAGA	12885	0.0	13.847566	7
CGCAGAG	13035	0.0	13.63758	8
CTAATAC	655	0.0	13.437228	3
TACATGG	10340	0.0	13.385083	2
GTATAAT	900	0.0	13.202441	1
AGAGTAC	12870	0.0	13.06062	11
GAGTACT	8375	0.0	13.004491	12
>>END_MODULE