FastQCFastQC Report
Mon 6 Feb 2017
SRR2077442_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077442_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755422
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38720.10310425832303267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55250.024.612081
GGTATCA23850.023.1567441
TATCAAC73300.018.2807462
TCAACGC73850.017.9348454
ATCAACG74350.017.8731773
CAACGCA75050.017.5587275
AACGCAG76900.017.106116
GTATAGA6600.015.6691721
CGTGCCG3100.015.61415210
GCGATAC2800.014.92936714
ACGCAGA88650.014.7405277
GTACATG101050.014.6980461
CGCAGAG90550.014.3826398
ATAATAC8150.014.3075113
TACATGG102750.014.2831692
AGAGTAC86400.013.87805511
GCACCGT5450.013.7233076
TGCGATA3051.8189894E-1213.70583213
GAGTACT41850.013.40591112
ACCGTGC6300.013.269318
Produced by FastQC (version 0.11.5)