FastQCFastQC Report
Mon 6 Feb 2017
SRR2077442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755422
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49260.13117034517026316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.025.6110121
GTATCAA67000.024.4001541
TATCAAC97900.016.4956262
GTACCGA3100.016.3235936
TCAACGC98450.016.2930344
ATCAACG99000.016.2238793
CAACGCA100200.015.9871545
AACGCAG103000.015.5932026
GCACCGT5800.015.552696
ACCGTGC8150.015.3874588
CGTGCCG4050.015.21122610
CGAATCG2750.015.1964937
GTACATG94950.014.1587471
GTGCCGA4450.013.84429311
TACATGG98100.013.5239542
ACGCAGA121300.013.2224087
GTATACG1505.273724E-513.2016981
CGCTTAT1200.001960097812.83583914
CGCAGAG124100.012.800158
GAGTACT77150.012.74891712
Produced by FastQC (version 0.11.5)