##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3755422
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.543148013725222	31.0	31.0	34.0	27.0	34.0
2	30.502181379349643	31.0	31.0	34.0	26.0	34.0
3	30.664218029292048	31.0	31.0	34.0	27.0	34.0
4	34.1415492053889	37.0	35.0	37.0	30.0	37.0
5	33.76707224913738	37.0	35.0	37.0	28.0	37.0
6	33.71319361712212	37.0	35.0	37.0	28.0	37.0
7	33.53470342347678	37.0	35.0	37.0	28.0	37.0
8	33.50293682041592	37.0	35.0	37.0	28.0	37.0
9	34.96412893144898	39.0	35.0	39.0	27.0	39.0
10	34.82589386758665	39.0	35.0	39.0	27.0	39.0
11	34.76729725713914	39.0	35.0	39.0	27.0	39.0
12	34.69354602492077	39.0	35.0	39.0	27.0	39.0
13	34.66050712809373	39.0	35.0	39.0	27.0	39.0
14	35.81706343521447	40.0	36.0	41.0	27.0	41.0
15	35.81593120560086	40.0	36.0	41.0	27.0	41.0
16	35.7583810820728	40.0	36.0	41.0	27.0	41.0
17	35.735242537323366	40.0	36.0	41.0	27.0	41.0
18	35.72698114885624	40.0	36.0	41.0	27.0	41.0
19	35.764881549929676	40.0	36.0	41.0	27.0	41.0
20	35.76622174551888	40.0	36.0	41.0	27.0	41.0
21	35.70563973902267	40.0	36.0	41.0	27.0	41.0
22	35.706035433567784	40.0	36.0	41.0	27.0	41.0
23	35.638409212067245	40.0	36.0	41.0	26.0	41.0
24	35.6252631528494	40.0	36.0	41.0	26.0	41.0
25	35.60397925985415	40.0	36.0	41.0	26.0	41.0
26	35.51967182383231	40.0	35.0	41.0	26.0	41.0
27	35.46503988100405	40.0	35.0	41.0	25.0	41.0
28	35.3981126488581	40.0	35.0	41.0	25.0	41.0
29	35.323710624265395	40.0	35.0	41.0	25.0	41.0
30	35.194029592413315	39.0	35.0	41.0	24.0	41.0
31	35.11535880654691	39.0	35.0	41.0	24.0	41.0
32	35.03078082835964	39.0	35.0	41.0	24.0	41.0
33	34.93750076555977	39.0	35.0	41.0	24.0	41.0
34	34.90710604560553	39.0	35.0	41.0	24.0	41.0
35	34.8452493488082	39.0	35.0	41.0	24.0	41.0
36	34.730398341384806	39.0	34.0	41.0	23.0	41.0
37	34.642059667329	39.0	34.0	41.0	22.0	41.0
38	34.5635585028793	39.0	34.0	41.0	22.0	41.0
39	34.471279659116874	39.0	34.0	41.0	21.0	41.0
40	34.37753706507551	39.0	34.0	41.0	19.0	41.0
41	34.315381600256906	39.0	34.0	41.0	19.0	41.0
42	34.198752097633765	39.0	34.0	41.0	18.0	41.0
43	34.29724009711825	39.0	34.0	41.0	18.0	41.0
44	34.22855966653015	39.0	34.0	41.0	16.0	41.0
45	34.157121889364234	39.0	34.0	41.0	15.0	41.0
46	34.00431615940898	39.0	34.0	41.0	9.0	41.0
47	33.855390419505454	39.0	34.0	41.0	7.0	41.0
48	33.69414169699171	39.0	33.0	41.0	2.0	41.0
49	33.537285024159736	39.0	33.0	41.0	2.0	41.0
50	33.38980599250896	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6209896911332962
1101	2	0.7410075090732242
1101	3	0.594318032689614
1101	4	0.633196552745602
1101	5	0.5516360147990298
1101	6	0.5731420307748749
1101	7	0.6686218254813596
1101	8	0.6069594576008228
1101	9	0.62589296077536
1101	10	0.7004176268845228
1101	11	0.7852378203347854
1101	12	0.7878017785042033
1101	13	0.7358353245214815
1101	14	0.8183932225425394
1101	15	0.8095833740081275
1101	16	0.8467567958688775
1101	17	0.7806015807436424
1101	18	0.808218924316435
1101	19	0.7818773807758106
1101	20	0.8760802992097396
1101	21	0.9319410360476255
1101	22	0.8845257811514742
1101	23	0.9307582822552476
1101	24	0.9155479864356053
1101	25	0.8962708228249952
1101	26	1.0067186000731567
1101	27	1.016679852461472
1101	28	0.949669975233256
1101	29	1.0145676667343793
1101	30	1.0768694956321667
1101	31	1.097641608320295
1101	32	1.0892960167064416
1101	33	1.0456327901042926
1101	34	1.0871302080749956
1101	35	1.004456517507002
1101	36	1.048127083753279
1101	37	1.1267853440719122
1101	38	1.1399976505953262
1101	39	1.1303381006060036
1101	40	1.1310496111092192
1101	41	1.029643028993327
1101	42	1.115378590231792
1101	43	1.1465301925324454
1101	44	1.1738793663773066
1101	45	1.1630641406749902
1101	46	1.1396886189751996
1101	47	1.1434132139988264
1101	48	1.2665750013733827
1101	49	1.2142355908356635
1101	50	1.285547235376164
1102	1	0.5788567777512306
1102	2	0.7006009524465568
1102	3	0.4971606115547047
1102	4	0.5511168585336463
1102	5	0.6113380722981532
1102	6	0.5825193487098232
1102	7	0.6130156486376137
1102	8	0.6611699270346705
1102	9	0.7504592146136346
1102	10	0.7991274197749121
1102	11	0.8268642505375396
1102	12	0.7994291272854852
1102	13	0.7363566308076059
1102	14	0.7267542462178653
1102	15	0.848241323001325
1102	16	0.8516831921698582
1102	17	0.9620248935140481
1102	18	0.8995629305461605
1102	19	0.9258584663617739
1102	20	0.9163801424416036
1102	21	0.9504333321189762
1102	22	0.8793511870094335
1102	23	0.9224921459249629
1102	24	0.867592608592993
1102	25	0.9338633245256034
1102	26	0.8237782704409966
1102	27	0.7823048990877624
1102	28	0.8099222651599902
1102	29	0.8758832956343667
1102	30	0.9206261652193461
1102	31	0.8514031419623933
1102	32	0.8166334325244975
1102	33	0.8167729406655369
1102	34	0.8600886056796355
1102	35	0.8010170312674489
1102	36	0.8170183387170766
1102	37	0.7722250298557825
1102	38	0.785166303817789
1102	39	0.8211540733890885
1102	40	0.852559794421424
1102	41	0.8421255704873261
1102	42	0.7982557675904971
1102	43	0.8277596372296685
1102	44	0.8366871599192578
1102	45	0.7754856641634191
1102	46	0.9211084481533263
1102	47	0.9624874321594135
1102	48	1.0342457016117592
1102	49	1.0726577946949618
1102	50	1.0306944032026664
1103	1	0.40137276560128043
1103	2	0.6027816678044431
1103	3	0.4903255948623162
1103	4	0.46713630804786277
1103	5	0.4695341924908405
1103	6	0.4809221304196569
1103	7	0.4844301226334906
1103	8	0.4910476323950377
1103	9	0.5578427076845358
1103	10	0.5634923777822607
1103	11	0.6029880435970227
1103	12	0.7243621667899518
1103	13	0.5868984506682295
1103	14	0.7193590925185944
1103	15	0.7283057778845716
1103	16	0.7075980523795877
1103	17	0.6885453565101969
1103	18	0.7047928614195911
1103	19	0.6258401670007174
1103	20	0.6824270959698566
1103	21	0.6537776009831902
1103	22	0.6960439424188323
1103	23	0.6876158208583334
1103	24	0.7249036722172448
1103	25	0.7007307581529076
1103	26	0.6754097414227971
1103	27	0.6417674553581421
1103	28	0.6411882837703615
1103	29	0.7617546852414065
1103	30	0.7354248284314551
1103	31	0.7491863840743207
1103	32	0.763902654671007
1103	33	0.7280053562293816
1103	34	0.671491426161225
1103	35	0.6576327256389547
1103	36	0.6364017048986099
1103	37	0.7665725354012878
1103	38	0.6859796924698003
1103	39	0.6771648120323377
1103	40	0.6562804403012379
1103	41	0.6431388228270407
1103	42	0.6921683989580956
1103	43	0.7247410252046791
1103	44	0.7238358507485643
1103	45	0.6913879576693276
1103	46	0.7293767390987469
1103	47	0.7070048106832942
1103	48	0.7520360209425618
1103	49	0.7058779030896574
1103	50	0.8196025586804296
1104	1	0.41876104343903364
1104	2	0.5802046531517782
1104	3	0.4669614355668372
1104	4	0.3936470891817194
1104	5	0.39594198094104627
1104	6	0.48771247392043193
1104	7	0.5144762845563662
1104	8	0.5035390180466237
1104	9	0.5148235668337477
1104	10	0.5404826401378671
1104	11	0.572894996945017
1104	12	0.5003423194336918
1104	13	0.6022599887742928
1104	14	0.6579108264385312
1104	15	0.5550230856097116
1104	16	0.6111478380532844
1104	17	0.714750525429892
1104	18	0.678115386499158
1104	19	0.5974175489491174
1104	20	0.6867092986131524
1104	21	0.6449558420878461
1104	22	0.6623629009604031
1104	23	0.756791100218777
1104	24	0.6381686847219186
1104	25	0.5528518069655775
1104	26	0.5905228577171968
1104	27	0.6131126554171047
1104	28	0.7062193245674777
1104	29	0.6787858160930682
1104	30	0.7958651689106659
1104	31	0.6598590617646423
1104	32	0.6767165297582451
1104	33	0.7125289761570457
1104	34	0.657542258494864
1104	35	0.7172794524075314
1104	36	0.7571312914909072
1104	37	0.8252987330347068
1104	38	0.8125220567456353
1104	39	0.8392216540659305
1104	40	0.7932708615012771
1104	41	0.8075200777871245
1104	42	0.8204749292674904
1104	43	0.7556507436308166
1104	44	0.7823732604870557
1104	45	0.6726796377211457
1104	46	0.7657145818740716
1104	47	0.7740954169275014
1104	48	0.8663092857039913
1104	49	0.8709989055620255
1104	50	0.7454126949346929
1105	1	0.4658248648827765
1105	2	0.5586831091097295
1105	3	0.5032639508189796
1105	4	0.46116606166376073
1105	5	0.4955607402576092
1105	6	0.5315850032244
1105	7	0.5336690019889332
1105	8	0.49632256207359404
1105	9	0.5924913089638437
1105	10	0.5551248841067675
1105	11	0.5842532230462254
1105	12	0.6236544032519689
1105	13	0.6562151448400826
1105	14	0.6767327666466159
1105	15	0.7178679084284667
1105	16	0.7311195961406298
1105	17	0.7172457618852093
1105	18	0.6412265090617666
1105	19	0.7436749299296253
1105	20	0.7815542697427986
1105	21	0.8010027787138441
1105	22	0.723095047722822
1105	23	0.8076629608668711
1105	24	0.7579761696457368
1105	25	0.6964846507891806
1105	26	0.6434351557064559
1105	27	0.6842548126618411
1105	28	0.747558439930728
1105	29	0.7675200643453906
1105	30	0.7802972073307259
1105	31	0.7983905765718546
1105	32	0.843410208697847
1105	33	0.8206320296017537
1105	34	0.8049842727075713
1105	35	0.7820427331449622
1105	36	0.8434082922425148
1105	37	0.8035067415713826
1105	38	0.8637132885555516
1105	39	0.8500842955582968
1105	40	0.9372407355375714
1105	41	0.7992421934545391
1105	42	0.8857156956577299
1105	43	0.8568010328370477
1105	44	0.9003347147381717
1105	45	0.9352082001361524
1105	46	0.9991144142073551
1105	47	0.9881739852498015
1105	48	0.9289798118054549
1105	49	0.9252588216532516
1105	50	1.0095944524438707
1106	1	0.44441348245115364
1106	2	0.5850204043468921
1106	3	0.4800185591279842
1106	4	0.5210713302222061
1106	5	0.5425683932876737
1106	6	0.61047916175756
1106	7	0.5579481329760654
1106	8	0.5408188546599888
1106	9	0.5577330848577091
1106	10	0.6066301428927829
1106	11	0.5772616639780281
1106	12	0.617792780785166
1106	13	0.5690758083877157
1106	14	0.6717813539204798
1106	15	0.7114491050618668
1106	16	0.711895099115111
1106	17	0.7025429962890257
1106	18	0.6497654704973357
1106	19	0.692724408937238
1106	20	0.7046918593742575
1106	21	0.7543602173613451
1106	22	0.7639753904906215
1106	23	0.7695534058786606
1106	24	0.70491281990288
1106	25	0.6736325991907819
1106	26	0.7246073580445085
1106	27	0.6607200655844068
1106	28	0.7148328801540274
1106	29	0.7266467455078214
1106	30	0.7748944307405665
1106	31	0.7282659476186026
1106	32	0.7634126823428389
1106	33	0.8146366470724047
1106	34	0.7597471081765903
1106	35	0.7235275919945039
1106	36	0.8208017728137165
1106	37	0.7768196240701855
1106	38	0.7506645058331998
1106	39	0.7819399568460526
1106	40	0.8954420902399463
1106	41	0.7728145032892613
1106	42	0.7985984998549824
1106	43	0.7547146201719883
1106	44	0.7759268625949574
1106	45	0.7390435714272101
1106	46	0.7742416084220807
1106	47	0.6826536341754519
1106	48	0.7560512604452541
1106	49	0.8718635148601948
1106	50	0.9313477170444813
1107	1	0.36358920181124077
1107	2	0.4386440111604841
1107	3	0.2791755570950869
1107	4	0.35933311720133787
1107	5	0.33862599393201265
1107	6	0.40062040669723586
1107	7	0.4737737477549402
1107	8	0.34385856405890536
1107	9	0.36653834206936864
1107	10	0.4736928213554066
1107	11	0.4494897449588393
1107	12	0.4201785298738514
1107	13	0.42899709044356626
1107	14	0.43428025836841044
1107	15	0.446961666867864
1107	16	0.569101599405684
1107	17	0.5134129402881555
1107	18	0.4990557699961755
1107	19	0.47085417833419996
1107	20	0.42299557172641045
1107	21	0.4352106863774807
1107	22	0.4047074245229254
1107	23	0.4917342264288038
1107	24	0.4189737755958305
1107	25	0.5292203941489788
1107	26	0.44105619025717147
1107	27	0.38618264324874474
1107	28	0.45069877529235214
1107	29	0.3788393403399297
1107	30	0.5628319867448184
1107	31	0.5975527503399718
1107	32	0.4465027968750306
1107	33	0.5149906845544407
1107	34	0.45716568037972394
1107	35	0.4712869651634364
1107	36	0.47123063743823934
1107	37	0.5115559132641749
1107	38	0.5233473626494529
1107	39	0.5151389523989778
1107	40	0.43527729224176426
1107	41	0.48152245300457963
1107	42	0.5301180833818933
1107	43	0.47843725889959643
1107	44	0.5538743321281174
1107	45	0.4309071215972011
1107	46	0.6135927899028815
1107	47	0.6835020296954397
1107	48	0.5971157130654916
1107	49	0.5992352071731446
1107	50	0.6825948186069937
1108	1	0.23836982321407874
1108	2	0.42576727500475187
1108	3	0.2505548763026759
1108	4	0.28090021050142155
1108	5	0.20316284074984026
1108	6	0.1630881222847833
1108	7	0.15683594482240437
1108	8	0.1545295897065202
1108	9	0.20703819234022092
1108	10	0.2419919341579373
1108	11	0.2970852324034112
1108	12	0.19703273552936906
1108	13	0.22587558150853226
1108	14	0.22032224469135997
1108	15	0.34722789185845926
1108	16	0.28243666812269197
1108	17	0.25413535202118
1108	18	0.2039330249738498
1108	19	0.3020446116879967
1108	20	0.2745296827746273
1108	21	0.24740123867778152
1108	22	0.19885836464860063
1108	23	0.2821079874276222
1108	24	0.2041882170164726
1108	25	0.2015951581238511
1108	26	0.2726958409250315
1108	27	0.2871801501539224
1108	28	0.24616156386463928
1108	29	0.2846206784104055
1108	30	0.35521131133189954
1108	31	0.367896278604114
1108	32	0.2874188104815545
1108	33	0.30050921659916696
1108	34	0.24687471571161979
1108	35	0.18842584148357133
1108	36	0.3459675056544782
1108	37	0.2353607549248551
1108	38	0.19599982786058945
1108	39	0.26877916372925625
1108	40	0.19140943284251222
1108	41	0.2402139757051316
1108	42	0.2229677547249338
1108	43	0.21996444235993096
1108	44	0.21688035536393357
1108	45	0.16782796718133142
1108	46	0.13222214362886575
1108	47	-0.0014606216998487298
1108	48	-0.027845569548418325
1108	49	-0.02907131139416208
1108	50	0.05072157852214332
1109	1	0.3149186766437495
1109	2	0.4048914963399035
1109	3	0.26556643592421736
1109	4	0.29622116868004866
1109	5	0.31441247305109243
1109	6	0.26385825179743705
1109	7	0.20358548793087294
1109	8	0.2542599439613724
1109	9	0.2798733770638009
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2211	5	-0.05467057249369134
2211	6	-0.11336109238094849
2211	7	-0.1967140895486068
2211	8	-0.16290055626918587
2211	9	-0.3194367283753863
2211	10	-0.20157231074851012
2211	11	-0.19607666949768543
2211	12	-0.2169143851042108
2211	13	-0.15988240036436707
2211	14	-0.2086034157230614
2211	15	-0.3035724616719335
2211	16	-0.1340224245981645
2211	17	-0.22315696331406798
2211	18	-0.2818994755604862
2211	19	-0.18856748113105937
2211	20	-0.19064381862042978
2211	21	-0.1694032971402777
2211	22	-0.2810406596768331
2211	23	-0.0647567409836256
2211	24	-0.11258685793544032
2211	25	-0.132986122095204
2211	26	-0.17835233047944143
2211	27	-0.052620613517945
2211	28	-0.13004243241520186
2211	29	-0.1153666129806723
2211	30	-0.18479279008280258
2211	31	-0.17040689103848194
2211	32	-0.20030496212677207
2211	33	-0.1552879308957884
2211	34	-0.07247934035645187
2211	35	-0.06083330438237056
2211	36	-0.18573094216224462
2211	37	-0.22643312299162233
2211	38	-0.22803792826120883
2211	39	-0.20354264135534095
2211	40	-0.07661657359266627
2211	41	0.008963795185124468
2211	42	-0.15819375436461058
2211	43	-0.2355759334682901
2211	44	-0.17087316694256316
2211	45	-0.14265807934373242
2211	46	-0.1150885414229208
2211	47	-0.10294001931178798
2211	48	-0.1524387496467341
2211	49	-0.08490600705186324
2211	50	-0.15851642483986694
2212	1	-0.9056840521003515
2212	2	-2.3536131943080463
2212	3	-1.2093273821618418
2212	4	-0.6841387020722394
2212	5	-0.5892016738172856
2212	6	-0.5173640666212904
2212	7	-0.5168038865367635
2212	8	-0.6060689199241978
2212	9	-0.6234565110621162
2212	10	-0.5716729335156927
2212	11	-0.6070941787708151
2212	12	-0.5473305508411883
2212	13	-2.10266758841955
2212	14	-1.4884711702855569
2212	15	-1.1185721482941204
2212	16	-0.8171222439785879
2212	17	-0.7636758599962548
2212	18	-0.8433129804349377
2212	19	-0.6487690685321326
2212	20	-0.7365146385877281
2212	21	-0.5807498531180997
2212	22	-0.659355199837627
2212	23	-0.6118035824201513
2212	24	-0.578168857513802
2212	25	-0.6614727351647645
2212	26	-0.7185054297860205
2212	27	-0.7010634106713312
2212	28	-0.6889489147777326
2212	29	-0.7514705860415702
2212	30	-0.6618860114357972
2212	31	-0.597661074632299
2212	32	-0.6160325399441788
2212	33	-0.6025689510260435
2212	34	-0.6680484629555536
2212	35	-0.6809589973746739
2212	36	-0.6874063739114007
2212	37	-0.7305189999082131
2212	38	-0.8500906099206276
2212	39	-0.7367925971975566
2212	40	-0.7143341575066984
2212	41	-0.8101314545769966
2212	42	-0.7136750172202255
2212	43	-0.8613982653410943
2212	44	-0.8310405676730213
2212	45	-0.8533283090211796
2212	46	-0.8505352757412084
2212	47	-0.8946175592389736
2212	48	-0.8464828618473845
2212	49	-0.8382993189995389
2212	50	-0.8564999810513001
2213	1	-0.8993835029009212
2213	2	-2.0437142767367433
2213	3	-1.1208277878040143
2213	4	-0.7639513691419353
2213	5	-0.8082180452682053
2213	6	-0.7442490251075782
2213	7	-0.797788763662318
2213	8	-0.7087327142842312
2213	9	-0.7697905453404772
2213	10	-0.7720232608459838
2213	11	-0.8146845074716396
2213	12	-0.8100243923393435
2213	13	-0.8242552434245098
2213	14	-0.943924720046553
2213	15	-0.9462361604291232
2213	16	-1.027305285290204
2213	17	-1.0290794028174872
2213	18	-0.9837447323735731
2213	19	-0.9064301886155732
2213	20	-1.1218839065328226
2213	21	-1.0800673754080918
2213	22	-1.0352550314830538
2213	23	-1.0169098246637347
2213	24	-0.9919969291135686
2213	25	-0.9320637067372886
2213	26	-1.016455993126307
2213	27	-1.0243891131698248
2213	28	-1.0012361268574494
2213	29	-1.0817248136344162
2213	30	-1.007183320724451
2213	31	-1.0177939347841871
2213	32	-0.9679853425918594
2213	33	-1.0647296213315798
2213	34	-1.0979475431321646
2213	35	-0.9738289868808252
2213	36	-1.096635822197399
2213	37	-0.9688875227725546
2213	38	-1.0301842938077783
2213	39	-1.0619233638060948
2213	40	-1.0678337431535283
2213	41	-0.9945302591956846
2213	42	-1.0096519586259802
2213	43	-1.0780898164273296
2213	44	-0.94351442741781
2213	45	-1.0272899724967175
2213	46	-1.03121152218349
2213	47	-0.9729666333469211
2213	48	-1.069658589985245
2213	49	-1.0828431849755944
2213	50	-1.1522792698969795
2214	1	-0.9589779448848255
2214	2	-1.9661566775419743
2214	3	-1.04306561784745
2214	4	-0.5772445386934066
2214	5	-0.7024395058586279
2214	6	-0.6229880326610058
2214	7	-0.6277763839855339
2214	8	-0.6941837715553945
2214	9	-0.8635804796948392
2214	10	-0.7870239484462118
2214	11	-0.7158479580739225
2214	12	-0.737026328105614
2214	13	-0.7684127975568344
2214	14	-0.8315617369951909
2214	15	-0.8925283922185656
2214	16	-0.8365726539324925
2214	17	-0.7733284320543845
2214	18	-0.9678722418091468
2214	19	-0.8451946415427685
2214	20	-0.9178452125808221
2214	21	-0.9694421090833032
2214	22	-0.9200864326841867
2214	23	-0.8645470381106151
2214	24	-0.8430610260083498
2214	25	-0.8082343902848805
2214	26	-0.9699131427623939
2214	27	-0.8220937378680873
2214	28	-0.8987457442831186
2214	29	-0.8739935326850485
2214	30	-0.8890530279659075
2214	31	-0.8821116372853268
2214	32	-0.7949152318740005
2214	33	-0.8446208756399258
2214	34	-0.9107867844983986
2214	35	-0.8673252712684842
2214	36	-0.9613992080012181
2214	37	-0.9650105032108343
2214	38	-0.9863378073156355
2214	39	-1.1093084274860345
2214	40	-1.052115836954954
2214	41	-1.0391175785490887
2214	42	-1.0156062576729283
2214	43	-1.0641300237031004
2214	44	-0.9990253830425004
2214	45	-1.1757849323885239
2214	46	-1.0371811618829838
2214	47	-0.9575906455877288
2214	48	-0.957764272487573
2214	49	-0.9926389414235999
2214	50	-1.051746601647011
2215	1	-0.4950855021345575
2215	2	-0.30988473693631136
2215	3	-0.5950930726206494
2215	4	-0.49798576981785203
2215	5	-0.6109041126898589
2215	6	-0.7000975050390608
2215	7	-0.7969201600831681
2215	8	-0.7460733588044164
2215	9	-0.9063100940792808
2215	10	-1.0422462970676278
2215	11	-0.9027576439474458
2215	12	-0.9182454843153138
2215	13	-0.8911374195946848
2215	14	-0.9474556167331656
2215	15	-0.9770223457505764
2215	16	-1.026952971403496
2215	17	-1.0618461186251764
2215	18	-1.0535673943362198
2215	19	-1.0757905471433418
2215	20	-1.0484246237750838
2215	21	-1.023918754967525
2215	22	-1.1003656211053752
2215	23	-1.133098714448174
2215	24	-1.1119321379827838
2215	25	-1.0914922869852788
2215	26	-1.1386423034493731
2215	27	-1.1512727423659754
2215	28	-1.2360893313554442
2215	29	-1.188967658182385
2215	30	-1.1896707808897489
2215	31	-1.1405788221271749
2215	32	-1.1135229073557014
2215	33	-1.119401452365075
2215	34	-1.1260373553812286
2215	35	-1.1912020547312352
2215	36	-1.1618830583244915
2215	37	-1.1838083302706437
2215	38	-1.0598879774954
2215	39	-1.0964551877018494
2215	40	-1.072339675258668
2215	41	-1.1584550538591287
2215	42	-1.2445499407136893
2215	43	-1.2127682304470184
2215	44	-1.2463667707706279
2215	45	-1.1056994518226304
2215	46	-1.1342001146229563
2215	47	-1.0026839000237615
2215	48	-1.064989716450306
2215	49	-0.9827944250385414
2215	50	-1.0234784249098396
2216	1	-1.1676868767403086
2216	2	-0.811936763352147
2216	3	-0.956275650380686
2216	4	-2.2880890780591727
2216	5	-1.6778223354378312
2216	6	-1.4442367913771008
2216	7	-1.3566926148390266
2216	8	-1.3137535391498716
2216	9	-1.3130902691900985
2216	10	-1.3509401476417509
2216	11	-1.5899475100826237
2216	12	-1.3819056106217147
2216	13	-1.481692961289049
2216	14	-1.5451860349121276
2216	15	-1.6806103221330773
2216	16	-1.623228454176406
2216	17	-1.5742925675603203
2216	18	-1.5428239145162337
2216	19	-1.5596377799041363
2216	20	-1.7075313991069265
2216	21	-1.7184747819665063
2216	22	-1.6349154448155403
2216	23	-1.554151346027119
2216	24	-1.6343684258525641
2216	25	-1.7272540513415109
2216	26	-1.7986370101067095
2216	27	-1.8922409152870543
2216	28	-1.9531777776321775
2216	29	-1.8859717338605506
2216	30	-1.8979019287379018
2216	31	-1.7041138552986226
2216	32	-1.7047516135150076
2216	33	-1.7501697649090744
2216	34	-1.8173792177474724
2216	35	-1.8149334503684784
2216	36	-1.854415188963408
2216	37	-1.8850319143137142
2216	38	-1.8374883320808664
2216	39	-2.0110004812499866
2216	40	-1.9480615781028376
2216	41	-2.008408847184924
2216	42	-2.053220553415663
2216	43	-1.919768994116879
2216	44	-1.8060183885912835
2216	45	-1.7674700211309329
2216	46	-1.7449266185658132
2216	47	-1.8548196072402732
2216	48	-1.8416933337797943
2216	49	-1.7344852859992592
2216	50	-1.7045613782428077
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92503.0
3	14898.0
4	25568.0
5	15002.0
6	11482.0
7	8957.0
8	7437.0
9	6713.0
10	6360.0
11	6293.0
12	6538.0
13	7102.0
14	7396.0
15	7942.0
16	8566.0
17	9519.0
18	10135.0
19	11482.0
20	13329.0
21	16525.0
22	20764.0
23	25445.0
24	29546.0
25	33407.0
26	36629.0
27	41523.0
28	48693.0
29	56427.0
30	65122.0
31	77414.0
32	94962.0
33	117626.0
34	147557.0
35	189322.0
36	246386.0
37	331531.0
38	525963.0
39	1373358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22187212788969	18.344434054949037	14.257997832676098	26.175695984485177
2	14.724626396892573	20.17735046888618	38.1850988006835	26.912924333537752
3	17.501005882984188	24.671493662338065	29.656758907889348	28.170741546788403
4	12.167865761074781	16.739682808506515	36.987522281639926	34.10492914877878
5	13.76902686196929	37.573432279275615	34.353959768980516	14.303581089774584
6	32.26908550455197	36.990320741952154	17.257925325349856	13.482668428146024
7	28.079842505548985	31.85421694802028	21.84867928458396	18.217261261846776
8	26.71380587782354	34.26426528004799	19.840524291440286	19.181404550688193
9	26.629988479799692	15.14705236030925	18.963208608852575	39.25975055103848
10	14.406854153459307	28.72223790827389	33.771382604109505	23.099525334157295
11	34.926267077327	22.68743836498796	23.606043121466058	18.780251436218983
12	23.470460636602926	24.549535330319447	30.369420853781758	21.610583179295872
13	28.22823094803387	21.560955156895727	26.007013278025543	24.203800617044866
14	22.417672816025647	20.573291353131587	27.196228494980996	29.81280733586177
15	23.750573717848823	28.53131160890597	23.788426442778853	23.929688230466358
16	23.652170669948678	27.652282556719264	25.24306734917597	23.452479424156085
17	22.535608619275887	27.37922267102074	26.663267377533685	23.42190133216969
18	22.583477705577597	26.865385747628878	27.66261891561903	22.888517631174494
19	23.438035746112924	26.610225529215587	27.655882054398433	22.295856670273054
20	23.677087706809942	25.9411544060135	27.62357979672258	22.758178090453978
21	23.823841264259414	25.873174448405024	27.56054091206982	22.742443375265744
22	23.531054045745236	25.61750315551452	27.50828106110536	23.343161737634883
23	23.3557339124486	25.73105132650571	27.43094146367973	23.482273297365953
24	23.111640106492878	25.87788162923237	27.45332411972084	23.55715414455391
25	23.26351132121411	25.706100759578103	27.586558712132316	23.443829207075474
26	23.147128446687137	26.114211062751508	27.55056046877023	23.188100021791126
27	23.244594371758467	26.14217680713961	27.347136343014938	23.266092478086982
28	23.224468636599273	26.007276826838904	27.494609511235712	23.273645025326108
29	23.402498845199148	26.078306952726955	27.330846315852288	23.188347886221614
30	23.368246194139445	26.097751925739203	27.380326067064765	23.153675813056587
31	23.489428557120316	25.67824945472601	27.324060567766878	23.508261420386802
32	23.271628681049737	26.034859896628546	27.277405634394075	23.416105787927638
33	23.406382672710905	25.899098158496116	27.255935480777936	23.438583688015044
34	23.510935969407733	25.890024007410496	27.3289505293935	23.270089493788277
35	23.47642777051267	25.931183631942428	27.237410876704228	23.35497772084067
36	23.51567951675021	26.03071448737276	27.04020156715265	23.413404428724384
37	23.490358653508633	25.798225508593614	27.301674724869674	23.409741113028083
38	23.582646621179116	25.873811864351254	27.171429507391615	23.372112007078012
39	23.637772691138462	26.13685722921968	26.960493324118794	23.264876755523066
40	23.612339423900664	25.83637781809573	27.252512615338237	23.29877014266537
41	23.355843674429046	26.002652880846984	27.084203801539374	23.557299643184596
42	23.62368488047951	26.0175832158953	26.990508024665715	23.368223878959473
43	23.445294729481365	25.854928792104687	27.015622012589002	23.68415446582495
44	23.563990482746316	26.050193260964218	26.812931641673003	23.57288461461647
45	23.55202423425165	26.063830070518136	26.659993513247514	23.7241521819827
46	23.68799455503494	25.88017348902752	26.616181089823815	23.815650866113717
47	23.599130642714073	26.125906623443782	26.594616098660655	23.68034663518149
48	23.558306549750892	26.27329639107315	26.48642891188978	23.68196814728618
49	23.739555096367386	26.040096713550014	26.491044943042326	23.729303247040278
50	23.75778142406518	26.109956335087066	26.457241244516588	23.675020996331167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	625.0
1	477.0
2	329.0
3	514.0
4	699.0
5	824.0
6	949.0
7	938.5
8	928.0
9	1204.5
10	1481.0
11	2260.0
12	3039.0
13	5682.5
14	8326.0
15	11799.0
16	15272.0
17	17313.5
18	19355.0
19	20752.5
20	22150.0
21	24256.5
22	26363.0
23	30612.0
24	34861.0
25	41052.0
26	47243.0
27	54948.5
28	62654.0
29	71104.0
30	79554.0
31	88581.5
32	97609.0
33	109367.0
34	121125.0
35	133393.0
36	145661.0
37	156293.5
38	166926.0
39	178919.5
40	190913.0
41	201499.5
42	212086.0
43	220287.5
44	228489.0
45	238653.5
46	248818.0
47	256120.5
48	263423.0
49	264899.0
50	266375.0
51	267472.0
52	268569.0
53	261791.5
54	255014.0
55	244185.0
56	233356.0
57	218067.0
58	202778.0
59	184266.0
60	165754.0
61	145357.0
62	124960.0
63	107179.5
64	89399.0
65	74355.5
66	59312.0
67	48475.0
68	37638.0
69	30250.5
70	22863.0
71	17754.0
72	12645.0
73	10487.0
74	8329.0
75	6368.0
76	4407.0
77	3421.0
78	2435.0
79	1858.5
80	1282.0
81	982.0
82	682.0
83	531.5
84	381.0
85	292.0
86	203.0
87	144.5
88	86.0
89	60.0
90	34.0
91	30.0
92	26.0
93	18.0
94	10.0
95	6.5
96	3.0
97	2.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014938401063848484
2	8.787294743440284E-4
3	3.994224883381948E-4
4	0.0023432785982507426
5	0.03869072503702647
6	0.02668142222099141
7	0.030116455620699884
8	0.023139876157726082
9	0.03054250627492729
10	0.03200705539883401
11	0.03909014752536466
12	0.039010263027697015
13	0.03727943224489818
14	0.042312155597959436
15	0.037252804079008967
16	0.043324025901749524
17	0.045161369348105225
18	0.04833012108892157
19	0.0504603743600586
20	0.04617323965189531
21	0.04691882829679328
22	0.04462880603032096
23	0.04393647371720142
24	0.0414334261236154
25	0.03983573617026263
26	0.04260506542274077
27	0.042684949920408416
28	0.042285527432070216
29	0.04068783747871744
30	0.037519085737901095
31	0.03584151128688068
32	0.031794030071720304
33	0.023486042314285853
34	0.019917868085131313
35	0.021089507384256682
36	0.022420915678717332
37	0.017468076823323717
38	0.018506575293003025
39	0.012967916788046723
40	0.007216232955976719
41	0.006896694965306162
42	0.006657041472303246
43	0.002130253271137039
44	0.003648058726822179
45	0.0020237406075801867
46	6.923323131195375E-4
47	1.0651266355685193E-4
48	3.195379906705558E-4
49	5.325633177842597E-5
50	1.0651266355685193E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3755422.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.96519445917803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75194717719731	45.25693490424
2	12.968338391248658	15.29361190104802
3	4.432543723244022	7.840974077696782
4	1.9612182007288366	4.625744503314204
5	1.0744088835873398	3.1676364374697923
6	0.6335656711699975	2.2414993801919127
7	0.44395151158517787	1.8324381047746232
8	0.2995570202520054	1.4130750360617146
9	0.22340122081849867	1.185560678518249
>10	1.1207861223875737	11.738549837336775
>50	0.06628328657538356	2.6697152057415336
>100	0.023273139251399004	2.2148645743763575
>500	5.89592216247341E-4	0.23627113905087513
>1k	1.3605973779441674E-4	0.2831242201791021
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4926	0.13117034517026316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.325633177842597E-5
5	0.0	0.0	0.0	0.0	5.325633177842597E-5
6	0.0	0.0	0.0	0.0	5.325633177842597E-5
7	0.0	0.0	0.0	0.0	5.325633177842597E-5
8	0.0	0.0	0.0	0.0	5.325633177842597E-5
9	0.0	0.0	0.0	0.0	5.325633177842597E-5
10	2.6628165889212984E-5	0.0	0.0	0.0	5.325633177842597E-5
11	2.6628165889212984E-5	0.0	0.0	0.0	5.325633177842597E-5
12	7.988449766763895E-5	0.0	0.0	0.0	1.331408294460649E-4
13	7.988449766763895E-5	0.0	0.0	0.0	2.1302532711370387E-4
14	7.988449766763895E-5	0.0	0.0	0.0	2.1302532711370387E-4
15	1.0651266355685193E-4	0.0	0.0	0.0	2.662816588921298E-4
16	1.0651266355685193E-4	0.0	0.0	0.0	2.9290982478134283E-4
17	1.0651266355685193E-4	0.0	0.0	0.0	3.195379906705558E-4
18	1.331408294460649E-4	0.0	0.0	2.6628165889212984E-5	3.461661565597688E-4
19	1.8639716122449088E-4	0.0	0.0	2.6628165889212984E-5	3.7279432244898176E-4
20	1.8639716122449088E-4	2.6628165889212984E-5	0.0	2.6628165889212984E-5	3.7279432244898176E-4
21	1.8639716122449088E-4	2.6628165889212984E-5	0.0	5.325633177842597E-5	3.994224883381947E-4
22	1.8639716122449088E-4	2.6628165889212984E-5	0.0	5.325633177842597E-5	3.994224883381947E-4
23	1.8639716122449088E-4	2.6628165889212984E-5	0.0	7.988449766763895E-5	4.526788201166207E-4
24	2.3965349300291686E-4	2.6628165889212984E-5	0.0	1.0651266355685193E-4	4.526788201166207E-4
25	2.662816588921298E-4	2.6628165889212984E-5	0.0	1.0651266355685193E-4	4.526788201166207E-4
26	2.662816588921298E-4	2.6628165889212984E-5	0.0	1.8639716122449088E-4	4.526788201166207E-4
27	2.662816588921298E-4	2.6628165889212984E-5	0.0	2.662816588921298E-4	4.526788201166207E-4
28	2.662816588921298E-4	2.6628165889212984E-5	0.0	6.923323131195376E-4	4.793069860058337E-4
29	2.662816588921298E-4	2.6628165889212984E-5	0.0	0.0013047801285714362	4.793069860058337E-4
30	2.662816588921298E-4	2.6628165889212984E-5	0.0	0.0019438561099125477	4.793069860058337E-4
31	2.662816588921298E-4	2.6628165889212984E-5	0.0	0.0038078277221574564	5.059351518950467E-4
32	2.662816588921298E-4	2.6628165889212984E-5	0.0	0.006177734486297412	5.591914836734727E-4
33	2.662816588921298E-4	5.325633177842597E-5	0.0	0.008281359591545238	5.591914836734727E-4
34	2.662816588921298E-4	5.325633177842597E-5	0.0	0.010784407185131259	5.591914836734727E-4
35	3.195379906705558E-4	5.325633177842597E-5	0.0	0.013793389930612325	5.591914836734727E-4
36	3.195379906705558E-4	5.325633177842597E-5	0.0	0.01749470498921293	5.591914836734727E-4
37	3.195379906705558E-4	5.325633177842597E-5	0.0	0.022261146683382055	5.591914836734727E-4
38	3.7279432244898176E-4	5.325633177842597E-5	0.0	0.027427010865889372	5.591914836734727E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3110	0.0	25.611012	1
GTATCAA	6700	0.0	24.400154	1
TATCAAC	9790	0.0	16.495626	2
GTACCGA	310	0.0	16.323593	6
TCAACGC	9845	0.0	16.293034	4
ATCAACG	9900	0.0	16.223879	3
CAACGCA	10020	0.0	15.987154	5
AACGCAG	10300	0.0	15.593202	6
GCACCGT	580	0.0	15.55269	6
ACCGTGC	815	0.0	15.387458	8
CGTGCCG	405	0.0	15.211226	10
CGAATCG	275	0.0	15.19649	37
GTACATG	9495	0.0	14.158747	1
GTGCCGA	445	0.0	13.844293	11
TACATGG	9810	0.0	13.523954	2
ACGCAGA	12130	0.0	13.222408	7
GTATACG	150	5.273724E-5	13.201698	1
CGCTTAT	120	0.0019600978	12.835839	14
CGCAGAG	12410	0.0	12.80015	8
GAGTACT	7715	0.0	12.748917	12
>>END_MODULE